Restriction map for pF1KA0698
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# NO CUT ENZYMES (  109 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AgeI        AhdI        Aor51HI     AscI        
AspEI       AvaI        AviII       AvrII       BbeI        
BbrPI       BcgI        BciVI       BclI        BglII       
BlnI        BsaAI       BsaHI       BseAI       BseRI       
BsiEI       BsiWI       BsmBI       BsmI        BsoBI       
BspDI       BspEI       BspHI       BsrBI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstZ17I     CfoI        ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     FbaI        FseI        FspI        HaeII       
HhaI        Hin1I       HinP1I      HincII      HindII      
HpaI        KasI        KspI        MfeI        MluI        
MroI        MunI        MvnI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspI        StuI        SwaI        
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AflII        2403  CTTAAG               C^TTAAG
Alw44I         53  GTGCAC               G^TGCAC
AocI         2121  CCTTAGG              CC^T[ACGT]AGG
ApaLI          53  GTGCAC               G^TGCAC
AseI         1066  ATTAAT               AT^TAAT
AsnI         1066  ATTAAT               AT^TAAT
AspI         2347  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbsI          610  GTCTTC               GAAGAC(2/6)
BbvCI         583  GCTGAGG              CCTCAGC(-5/-2)
BfaI         1723  CTAG                 C^TAG
BfrI         2403  CTTAAG               C^TTAAG
BlpI         1753  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1753  GCTGAGC              GC^T[ACGT]AGC
BpuAI         610  GTCTTC               GAAGAC(2/6)
BsaI         2368  GAGACC               GGTCTC(1/5)
BsaWI        1445  ACCGGA               [AT]^CCGG[AT]
Bsp1407I      689  TGTACA               T^GTACA
BsrFI         772  GCCGGT               [GA]^CCGG[CT]
BsrGI         689  TGTACA               T^GTACA
Bsu36I       2121  CCTTAGG              CC^T[ACGT]AGG
CelII        1753  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        772  GCCGGT               [GA]^CCGG[CT]
Eco81I       2121  CCTTAGG              CC^T[ACGT]AGG
EcoRI         928  GAATTC               G^AATTC
EcoRV        1657  GATATC               GAT^ATC
FauI          864  CCCGC                CCCGC(4/6)
HgaI         2618  GACGC                GACGC(5/10)
HindIII      2653  AAGCTT               A^AGCTT
MaeI         1723  CTAG                 C^TAG
PflFI        2347  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        2029  GGGTCCC              [GA]G^G[AT]CC[CT]
PshBI        1066  ATTAAT               AT^TAAT
PvuII         992  CAGCTG               CAG^CTG
SacI          456  GAGCTC               GAGCT^C
SapI          453  GAAGAGC              GCTCTTC(1/4)
SfiI          853  GGCCTGGTGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI         2403  CTTAAG               C^T[CT][GA]AG
SspBI         689  TGTACA               T^GTACA
Tth111I      2347  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         2349  CCATGTCCATGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

Acc65I        220  GGTACC               G^GTACC
             2591  GGTACC              
AlwNI         130  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1151  CAGAGACTG           
Asp700I       499  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              928  GAATTCTTTC          
Asp718I       220  GGTACC               G^GTACC
             2591  GGTACC              
AspHI          53  GTGCAC               G[AT]GC[AT]^C
              456  GAGCTC              
BcnI          579  CCGGG                CC^[GC]GG
             2250  CCGGG               
BpmI          459  CTCCAG               CTGGAG(16/14)
             1921  CTGGAG              
BsaBI         547  GATAGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2185  GATGTGGATC          
BsiHKAI        53  GTGCAC               G[AT]GC[AT]^C
              456  GAGCTC              
BstXI         599  CCATTCAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1347  CCAGTGTCCTGG        
KpnI          220  GGTACC               GGTAC^C
             2591  GGTACC              
MaeII          63  ACGT                 A^CGT
             1576  ACGT                
MamI          547  GATAGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2185  GATGTGGATC          
NciI          579  CCGGG                CC^[GC]GG
             2250  CCGGG               
PflMI        1594  CCAAGGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2181  CCAAGATGTGG         
PstI          824  CTGCAG               CTGCA^G
             1850  CTGCAG              
ScaI          341  AGTACT               AGT^ACT
              400  AGTACT              
SexAI         216  ACCTGGT              A^CC[AT]GGT
              223  ACCTGGT             
Van91I       1594  CCAAGGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2181  CCAAGATGTGG         
XmnI          499  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              928  GAATTCTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   89 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        ApaI        ApoI        AvaII       BalI        
BamHI       BanI        BanII       BbvI        BglI        
BmyI        BsaJI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspLU11I    BspMI       
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     EcoT22I     Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NcoI        NdeII       NlaIII      NlaIV       NsiI        
NspI        PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SphI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       
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