Restriction map for pF1KA0698
 (Linear) (Six-base) MAPSORT of: KIAA0698.seq  Check: 8259  from: 1  to: 2673



KIAA0698.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1142    2673 
   Size:       1142    1531
 
Acc65I G'GTAC_C

Cuts at:      0     220    2591    2673 
   Size:        220    2371      82

  Fragments arranged by size: 

                2371     220      82
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1183    2673 
   Size:       1183    1490
 
AflII C'TTAA_G

Cuts at:      0    2403    2673 
   Size:       2403     270
 
AflIII A'CryG_T

Cuts at:      0      47      63    1103    1871    2673 
   Size:         47      16    1040     768     802

  Fragments arranged by size: 

                1040     802     768      47      16
 
AleI CACnn'nnGTG

Cuts at:      0     160     187    2673 
   Size:        160      27    2486

  Fragments arranged by size: 

                2486     160      27
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1207    2104    2673 
   Size:       1207     897     569

  Fragments arranged by size: 

                1207     897     569
 
AlwNI CAG_nnn'CTG

Cuts at:      0     135    1156    2673 
   Size:        135    1021    1517

  Fragments arranged by size: 

                1517    1021     135
 
ApaI G_GGCC'C

Cuts at:      0     865    1814    1895    2673 
   Size:        865     949      81     778

  Fragments arranged by size: 

                 949     865     778      81
 
ApaLI G'TGCA_C

Cuts at:      0      53    2673 
   Size:         53    2620
 
ApoI r'AATT_y

Cuts at:      0      30     411     846     928    1957    2673 
   Size:         30     381     435      82    1029     716

  Fragments arranged by size: 

                1029     716     435     381      82      30
 
AseI AT'TA_AT

Cuts at:      0    1067    2673 
   Size:       1067    1606
 
BamHI G'GATC_C

Cuts at:      0     789    1583    1857    2037    2673 
   Size:        789     794     274     180     636

  Fragments arranged by size: 

                 794     789     636     274     180
 
BanI G'GyrC_C

Cuts at:      0     204     220     886    1130    2591    2673 
   Size:        204      16     666     244    1461      82

  Fragments arranged by size: 

                1461     666     244     204      82      16
 
BanII G_rGCy'C

Cuts at:      0     460     865    1526    1814    1895    1928    2673 
   Size:        460     405     661     288      81      33     745

  Fragments arranged by size: 

                 745     661     460     405     288      81      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     603    2673 
   Size:        603    2070
 
BbvCI CC'TCA_GC

Cuts at:      0     584    2673 
   Size:        584    2089
 
BfrBI ATG'CAT

Cuts at:      0       3     534    1059    2091    2673 
   Size:          3     531     525    1032     582

  Fragments arranged by size: 

                1032     582     531     525       3
 
BglI GCCn_nnn'nGGC

Cuts at:      0     860    1301    1913    2673 
   Size:        860     441     612     760

  Fragments arranged by size: 

                 860     760     612     441
 
BlpI GC'TnA_GC

Cuts at:      0    1754    2673 
   Size:       1754     919
 
Bme1580I G_kGCm'C

Cuts at:      0      57     209     478     865    1150    1814    1895    2450 
   Size:         57     152     269     387     285     664      81     555

Cuts at:   2450    2673 
   Size:        223

  Fragments arranged by size: 

                 664     555     387     285     269     223     152      81
                  57
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     413    1341    2673 
   Size:        413     928    1332

  Fragments arranged by size: 

                1332     928     413
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     444    1942    2673 
   Size:        444    1498     731

  Fragments arranged by size: 

                1498     731     444
 
Bpu10I CC'TnA_GC

Cuts at:      0      38     584     785    1507    2673 
   Size:         38     546     201     722    1166

  Fragments arranged by size: 

                1166     722     546     201      38
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2362    2673 
   Size:       2362     311
 
BsaBI GATnn'nnATC

Cuts at:      0     551    2189    2673 
   Size:        551    1638     484

  Fragments arranged by size: 

                1638     551     484
 
BsaWI w'CCGG_w

Cuts at:      0    1445    2673 
   Size:       1445    1228
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     610     640    2138    2168    2673 
   Size:        610      30    1498      30     505

  Fragments arranged by size: 

                1498     610     505      30      30
 
BseYI C'CCAG_C

Cuts at:      0     629    1451    2484    2673 
   Size:        629     822    1033     189

  Fragments arranged by size: 

                1033     822     629     189
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     897     917    1213    1238    2673 
   Size:        897      20     296      25    1435

  Fragments arranged by size: 

                1435     897     296      25      20
 
BsiHKAI G_wGCw'C

Cuts at:      0      57     460    2673 
   Size:         57     403    2213

  Fragments arranged by size: 

                2213     403      57
 
Bsp1286I G_dGCh'C

Cuts at:      0      57     209     460     478     865    1150    1526    1814 
   Size:         57     152     251      18     387     285     376     288

Cuts at:   1814    1895    1928    2450    2673 
   Size:         81      33     522     223

  Fragments arranged by size: 

                 522     387     376     288     285     251     223     152
                  81      57      33      18
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     326    1097    1142    2673 
   Size:        326     771      45    1531

  Fragments arranged by size: 

                1531     771     326      45
 
BsrDI GCAATG_nn'

Cuts at:      0      99     984    2478    2673 
   Size:         99     885    1494     195

  Fragments arranged by size: 

                1494     885     195      99
 
BsrFI r'CCGG_y

Cuts at:      0     772    2673 
   Size:        772    1901
 
BsrGI T'GTAC_A

Cuts at:      0     689    2673 
   Size:        689    1984
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     606    1354    2673 
   Size:        606     748    1319

  Fragments arranged by size: 

                1319     748     606
 
BstYI r'GATC_y

Cuts at:      0     789     828    1583    1838    1857    2037    2190    2265 
   Size:        789      39     755     255      19     180     153      75

Cuts at:   2265    2495    2673 
   Size:        230     178

  Fragments arranged by size: 

                 789     755     255     230     180     178     153      75
                  39      19
 
Bsu36I CC'TnA_GG

Cuts at:      0    2122    2673 
   Size:       2122     551
 
BtgI C'CryG_G

Cuts at:      0     123     144     299     651    1929    2025    2174    2673 
   Size:        123      21     155     352    1278      96     149     499

  Fragments arranged by size: 

                1278     499     352     155     149     123      96      21
            
 
BtsI GCAGTG_nn'

Cuts at:      0     779    1859    2331    2450    2673 
   Size:        779    1080     472     119     223

  Fragments arranged by size: 

                1080     779     472     223     119
 
EaeI y'GGCC_r

Cuts at:      0    1250    1356    1591    1741    2171    2303    2673 
   Size:       1250     106     235     150     430     132     370

  Fragments arranged by size: 

                1250     430     370     235     150     132     106
 
EarI CTCTTCn'nnn_

Cuts at:      0     448     946    2380    2673 
   Size:        448     498    1434     293

  Fragments arranged by size: 

                1434     498     448     293
 
EcoICRI GAG'CTC

Cuts at:      0     458    2673 
   Size:        458    2215
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     444    1183    1942    2673 
   Size:        444     739     759     731

  Fragments arranged by size: 

                 759     739     731     444
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1891    1892    2030    2673 
   Size:       1891       1     138     643

  Fragments arranged by size: 

                1891     643     138       1
 
EcoRI G'AATT_C

Cuts at:      0     928    2673 
   Size:        928    1745
 
EcoRV GAT'ATC

Cuts at:      0    1659    2673 
   Size:       1659    1014
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     688     720    2673 
   Size:        688      32    1953

  Fragments arranged by size: 

                1953     688      32
 
HindIII A'AGCT_T

Cuts at:      0    2653    2673 
   Size:       2653      20
 
KpnI G_GTAC'C

Cuts at:      0     224    2595    2673 
   Size:        224    2371      78

  Fragments arranged by size: 

                2371     224      78
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     932     983    1035    2673 
   Size:        932      51      52    1638

  Fragments arranged by size: 

                1638     932      52      51
 
MscI TGG'CCA

Cuts at:      0    1252    1358    1743    2173    2305    2673 
   Size:       1252     106     385     430     132     368

  Fragments arranged by size: 

                1252     430     385     368     132     106
 
MslI CAynn'nnrTG

Cuts at:      0     158     160     187     604     808    1712    2210    2336 
   Size:        158       2      27     417     204     904     498     126

Cuts at:   2336    2354    2673 
   Size:         18     319

  Fragments arranged by size: 

                 904     498     417     319     204     158     126      27
                  18       2
 
MspA1I CmG'CkG

Cuts at:      0     752     867     994    2673 
   Size:        752     115     127    1679

  Fragments arranged by size: 

                1679     752     127     115
 
NcoI C'CATG_G

Cuts at:      0     123     299     651    1929    2025    2174    2673 
   Size:        123     176     352    1278      96     149     499

  Fragments arranged by size: 

                1278     499     352     176     149     123      96
 
NsiI A_TGCA'T

Cuts at:      0       5     536    1061    2093    2673 
   Size:          5     531     525    1032     580

  Fragments arranged by size: 

                1032     580     531     525       5
 
NspI r_CATG'y

Cuts at:      0       7      51     270     645    1063    1107    1711    1875 
   Size:          7      44     219     375     418      44     604     164

Cuts at:   1875    2095    2169    2493    2673 
   Size:        220      74     324     180

  Fragments arranged by size: 

                 604     418     375     324     220     219     180     164
                  74      44      44       7
 
PasI CC'CwG_GG

Cuts at:      0     209    1807    2275    2314    2673 
   Size:        209    1598     468      39     359

  Fragments arranged by size: 

                1598     468     359     209      39
 
PciI A'CATG_T

Cuts at:      0      47    1103    1871    2673 
   Size:         47    1056     768     802

  Fragments arranged by size: 

                1056     802     768      47
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1600    2187    2673 
   Size:       1600     587     486

  Fragments arranged by size: 

                1600     587     486
 
PfoI T'CCnGG_A

Cuts at:      0    1834    1919    2636    2673 
   Size:       1834      85     717      37

  Fragments arranged by size: 

                1834     717      85      37
 
PpuMI rG'GwC_Cy

Cuts at:      0    2030    2673 
   Size:       2030     643
 
PspOMI G'GGCC_C

Cuts at:      0     861    1810    1891    2673 
   Size:        861     949      81     782

  Fragments arranged by size: 

                 949     861     782      81
 
PstI C_TGCA'G

Cuts at:      0     828    1854    2673 
   Size:        828    1026     819

  Fragments arranged by size: 

                1026     828     819
 
PvuII CAG'CTG

Cuts at:      0     994    2673 
   Size:        994    1679
 
SacI G_AGCT'C

Cuts at:      0     460    2673 
   Size:        460    2213
 
SanDI GG'GwC_CC

Cuts at:      0    2030    2673 
   Size:       2030     643
 
SapI GCTCTTCn'nnn_

Cuts at:      0     448    2673 
   Size:        448    2225
 
ScaI AGT'ACT

Cuts at:      0     343     402    2673 
   Size:        343      59    2271

  Fragments arranged by size: 

                2271     343      59
 
SexAI A'CCwGG_T

Cuts at:      0     216     223    2673 
   Size:        216       7    2450

  Fragments arranged by size: 

                2450     216       7
 
SfcI C'TryA_G

Cuts at:      0     824    1850    2056    2673 
   Size:        824    1026     206     617

  Fragments arranged by size: 

                1026     824     617     206
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     860    2673 
   Size:        860    1813
 
SmlI C'TyrA_G

Cuts at:      0    2403    2673 
   Size:       2403     270
 
SphI G_CATG'C

Cuts at:      0       7     270     645    1063    1711    2095    2493    2673 
   Size:          7     263     375     418     648     384     398     180

  Fragments arranged by size: 

                 648     418     398     384     375     263     180       7
            
 
StyI C'CwwG_G

Cuts at:      0     123     299     651     890    1272    1594    1895    1929 
   Size:        123     176     352     239     382     322     301      34

Cuts at:   1929    2025    2142    2174    2673 
   Size:         96     117      32     499

  Fragments arranged by size: 

                 499     382     352     322     301     239     176     123
                 117      96      34      32
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     580     883    2673 
   Size:        580     303    1790

  Fragments arranged by size: 

                1790     580     303
 
TatI w'GTAC_w

Cuts at:      0     341     400     689     812    2673 
   Size:        341      59     289     123    1861

  Fragments arranged by size: 

                1861     341     289     123      59
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2138    2170    2673 
   Size:       2138      32     503

  Fragments arranged by size: 

                2138     503      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2350    2673 
   Size:       2350     323
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2356    2673 
   Size:       2356     317
 
XmnI GAAnn'nnTTC

Cuts at:      0     503     932    2673 
   Size:        503     429    1741

  Fragments arranged by size: 

                1741     503     429
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI    AflII   AflIII     AleI     AlfI    AlwNI
     ApaI    ApaLI     ApoI     AseI    BamHI     BanI    BanII     BbsI
    BbvCI    BfrBI     BglI     BlpI Bme1580I     BmrI     BpmI   Bpu10I
     BsaI    BsaBI    BsaWI    BsaXI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspMI    BsrDI    BsrFI    BsrGI    BstXI    BstYI   Bsu36I     BtgI
     BtsI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV
    Hin4I  HindIII     KpnI     MmeI     MscI     MslI   MspA1I     NcoI
     NsiI     NspI     PasI     PciI    PflMI     PfoI    PpuMI   PspOMI
     PstI    PvuII     SacI    SanDI     SapI     ScaI    SexAI     SfcI
     SfiI     SmlI     SphI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI     AgeI     AhdI     AloI     AscI
    AsiSI     AvaI    AvrII     BaeI     BbeI     BcgI    BciVI     BclI
    BglII    BmgBI     BmtI     BplI    BpuEI    BsaAI    BsaHI    BseRI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI    EcoNI     FalI     FseI
     FspI    FspAI    HaeII   HincII     HpaI     KasI     MfeI     MluI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     PacI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI     SbfI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     SspI     StuI     SwaI     XbaI     XhoI
     XmaI     ZraI
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