Restriction map for pF1KB7327
 (Linear) (Six-base) MAPSORT of: KIBB7327.seq  Check: 6004  from: 1  to: 2514



KIBB7327.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AccI GT'mk_AC

Cuts at:      0     178     198     841    1518    2514 
   Size:        178      20     643     677     996

  Fragments arranged by size: 

                 996     677     643     178      20
 
Acc65I G'GTAC_C

Cuts at:      0     191     674    2514 
   Size:        191     483    1840

  Fragments arranged by size: 

                1840     483     191
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     502    1292    1375    1682    1693    1908    2089    2420 
   Size:        502     790      83     307      11     215     181     331

Cuts at:   2420    2507    2514 
   Size:         87       7

  Fragments arranged by size: 

                 790     502     331     307     215     181      87      83
                  11       7
 
AflIII A'CryG_T

Cuts at:      0    1469    2514 
   Size:       1469    1045
 
AleI CACnn'nnGTG

Cuts at:      0      70    2514 
   Size:         70    2444
 
ApoI r'AATT_y

Cuts at:      0     185     238     339     437     586     865    1759    1788 
   Size:        185      53     101      98     149     279     894      29

Cuts at:   1788    2289    2481    2514 
   Size:        501     192      33

  Fragments arranged by size: 

                 894     501     279     192     185     149     101      98
                  53      33      29
 
AseI AT'TA_AT

Cuts at:      0     725    2042    2311    2514 
   Size:        725    1317     269     203

  Fragments arranged by size: 

                1317     725     269     203
 
BamHI G'GATC_C

Cuts at:      0    1477    1989    2514 
   Size:       1477     512     525

  Fragments arranged by size: 

                1477     525     512
 
BanI G'GyrC_C

Cuts at:      0     191     674    2514 
   Size:        191     483    1840

  Fragments arranged by size: 

                1840     483     191
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1356    2514 
   Size:       1356    1158
 
BclI T'GATC_A

Cuts at:      0    1592    2234    2421    2514 
   Size:       1592     642     187      93

  Fragments arranged by size: 

                1592     642     187      93
 
BglII A'GATC_T

Cuts at:      0     513    1430    2385    2514 
   Size:        513     917     955     129

  Fragments arranged by size: 

                 955     917     513     129
 
BmgBI CAC'GTC

Cuts at:      0     992    2514 
   Size:        992    1522
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     595    2088    2514 
   Size:        595    1493     426

  Fragments arranged by size: 

                1493     595     426
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      26     677    2514 
   Size:         26     651    1837

  Fragments arranged by size: 

                1837     651      26
 
Bpu10I CC'TnA_GC

Cuts at:      0     700    2514 
   Size:        700    1814
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1007    2514 
   Size:       1007    1507
 
BsaBI GATnn'nnATC

Cuts at:      0    2384    2514 
   Size:       2384     130
 
BsaWI w'CCGG_w

Cuts at:      0     605    2514 
   Size:        605    1909
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1134    1164    2514 
   Size:       1134      30    1350

  Fragments arranged by size: 

                1350    1134      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     477     480     674     818    2514 
   Size:        477       3     194     144    1696

  Fragments arranged by size: 

                1696     477     194     144       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1303    2514 
   Size:       1303    1211
 
BsmI GAATG_Cn'

Cuts at:      0    1823    2436    2514 
   Size:       1823     613      78

  Fragments arranged by size: 

                1823     613      78
 
BspEI T'CCGG_A

Cuts at:      0     605    2514 
   Size:        605    1909
 
BsrBI CCG'CTC

Cuts at:      0     135    2514 
   Size:        135    2379
 
BsrDI GCAATG_nn'

Cuts at:      0      53     773    1032    1183    2514 
   Size:         53     720     259     151    1331

  Fragments arranged by size: 

                1331     720     259     151      53
 
BssSI C'ACGA_G

Cuts at:      0    2021    2514 
   Size:       2021     493
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1317    2514 
   Size:       1317    1197
 
BstBI TT'CG_AA

Cuts at:      0     535    2514 
   Size:        535    1979
 
BstYI r'GATC_y

Cuts at:      0     513    1430    1477    1989    2100    2298    2385    2514 
   Size:        513     917      47     512     111     198      87     129

  Fragments arranged by size: 

                 917     513     512     198     129     111      87      47
            
 
Bsu36I CC'TnA_GG

Cuts at:      0    2303    2514 
   Size:       2303     211
 
BtgI C'CryG_G

Cuts at:      0      17    2514 
   Size:         17    2497
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     684    2514 
   Size:        684    1830
 
BtsI GCAGTG_nn'

Cuts at:      0      65    2514 
   Size:         65    2449
 
ClaI AT'CG_AT

Cuts at:      0    1769    2183    2514 
   Size:       1769     414     331

  Fragments arranged by size: 

                1769     414     331
 
DraI TTT'AAA

Cuts at:      0     320    2514 
   Size:        320    2194
 
DraIII CAC_nnn'GTG

Cuts at:      0    1851    2514 
   Size:       1851     663
 
EaeI y'GGCC_r

Cuts at:      0      83    2514 
   Size:         83    2431
 
EarI CTCTTCn'nnn_

Cuts at:      0      89     353     384    2514 
   Size:         89     264      31    2130

  Fragments arranged by size: 

                2130     264      89      31
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      26     502     677    1292    1375    1682    1693    1908 
   Size:         26     476     175     615      83     307      11     215

Cuts at:   1908    2089    2420    2507    2514 
   Size:        181     331      87       7

  Fragments arranged by size: 

                 615     476     331     307     215     181     175      87
                  83      26      11       7
 
EcoO109I rG'GnC_Cy

Cuts at:      0     814    2514 
   Size:        814    1700
 
EcoRI G'AATT_C

Cuts at:      0     865    2514 
   Size:        865    1649
 
EcoRV GAT'ATC

Cuts at:      0     888    2514 
   Size:        888    1626
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     124    2310    2514 
   Size:        124    2186     204

  Fragments arranged by size: 

                2186     204     124
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     160     192     409     441     469     501     544     576 
   Size:        160      32     217      32      28      32      43      32

Cuts at:    576    1134    1166    1382    1414    1719    1751    1797    1829 
   Size:        558      32     216      32     305      32      46      32

Cuts at:   1829    2354    2386    2514 
   Size:        525      32     128

  Fragments arranged by size: 

                 558     525     305     217     216     160     128      46
                  43      32      32      32      32      32      32      32
                  32      32      28
 
HincII GTy'rAC

Cuts at:      0     179    1519    2514 
   Size:        179    1340     995

  Fragments arranged by size: 

                1340     995     179
 
HindIII A'AGCT_T

Cuts at:      0    1227    2514 
   Size:       1227    1287
 
KpnI G_GTAC'C

Cuts at:      0     195     678    2514 
   Size:        195     483    1836

  Fragments arranged by size: 

                1836     483     195
 
MfeI C'AATT_G

Cuts at:      0     344    1460    1496    2514 
   Size:        344    1116      36    1018

  Fragments arranged by size: 

                1116    1018     344      36
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1862    2078    2514 
   Size:       1862     216     436

  Fragments arranged by size: 

                1862     436     216
 
MslI CAynn'nnrTG

Cuts at:      0      70    1891    2514 
   Size:         70    1821     623

  Fragments arranged by size: 

                1821     623      70
 
MspA1I CmG'CkG

Cuts at:      0       7      19      26    2209    2514 
   Size:          7      12       7    2183     305

  Fragments arranged by size: 

                2183     305      12       7       7
 
NdeI CA'TA_TG

Cuts at:      0    1313    2514 
   Size:       1313    1201
 
NspI r_CATG'y

Cuts at:      0     162    1473    1881    1974    2514 
   Size:        162    1311     408      93     540

  Fragments arranged by size: 

                1311     540     408     162      93
 
PciI A'CATG_T

Cuts at:      0    1469    2514 
   Size:       1469    1045
 
PpuMI rG'GwC_Cy

Cuts at:      0     814    2514 
   Size:        814    1700
 
PshAI GACnn'nnGTC

Cuts at:      0    1356    2514 
   Size:       1356    1158
 
PstI C_TGCA'G

Cuts at:      0    1682    2055    2514 
   Size:       1682     373     459

  Fragments arranged by size: 

                1682     459     373
 
PvuII CAG'CTG

Cuts at:      0    2209    2514 
   Size:       2209     305
 
SacII CC_GC'GG

Cuts at:      0      20    2514 
   Size:         20    2494
 
SalI G'TCGA_C

Cuts at:      0     177    1517    2514 
   Size:        177    1340     997

  Fragments arranged by size: 

                1340     997     177
 
SfcI C'TryA_G

Cuts at:      0    1652    1678    1739    2051    2514 
   Size:       1652      26      61     312     463

  Fragments arranged by size: 

                1652     463     312      61      26
 
SpeI A'CTAG_T

Cuts at:      0    1252    2514 
   Size:       1252    1262
 
SspI AAT'ATT

Cuts at:      0     411     945    2514 
   Size:        411     534    1569

  Fragments arranged by size: 

                1569     534     411
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1031    1634    2514 
   Size:       1031     603     880

  Fragments arranged by size: 

                1031     880     603
 
TatI w'GTAC_w

Cuts at:      0    2017    2514 
   Size:       2017     497
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     872     904    2514 
   Size:        872      32    1610

  Fragments arranged by size: 

                1610     872      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     601    2514 
   Size:        601    1913
 
XbaI T'CTAG_A

Cuts at:      0    2329    2388    2514 
   Size:       2329      59     126

  Fragments arranged by size: 

                2329     126      59
 
XmnI GAAnn'nnTTC

Cuts at:      0     515    2514 
   Size:        515    1999
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI   AflIII     AleI     ApoI     AseI    BamHI
     BanI     BbsI     BclI    BglII    BmgBI     BmrI     BpmI   Bpu10I
     BsaI    BsaBI    BsaWI    BsaXI    BseRI     BsgI     BsmI    BspEI
    BsrBI    BsrDI    BssSI   BstAPI    BstBI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     ClaI     DraI   DraIII     EaeI     EarI  Eco57MI
 EcoO109I    EcoRI    EcoRV     FalI    Hin4I   HincII  HindIII     KpnI
     MfeI     MmeI     MslI   MspA1I     NdeI     NspI     PciI    PpuMI
    PshAI     PstI    PvuII    SacII     SalI     SfcI     SpeI     SspI
    TaqII     TatI     TstI  Tth111I     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII     AgeI     AhdI     AlfI
     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII
     BaeI    BanII     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI
     BlpI Bme1580I     BmtI     BplI    BpuEI    BsaAI    BsaHI    BseYI
    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspHI    BspMI    BsrFI
    BsrGI   BssHII   BstEII    BstXI  BstZ17I    CspCI     DrdI     EagI
     EciI  EcoICRI    EcoNI     FseI     FspI    FspAI    HaeII     HpaI
     KasI     MluI     MscI     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI
     PmlI     PpiI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SphI     SrfI     StuI
     StyI     SwaI     XcmI     XhoI     XmaI     ZraI
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