Restriction map for pF1KE0110
 (Linear) (Six-base) MAPSORT of: KIEE0110.seq  Check: 3449  from: 1  to: 777



KIEE0110.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     224     777 
   Size:        224     553
 
AleI CACnn'nnGTG

Cuts at:      0     526     777 
   Size:        526     251
 
ApoI r'AATT_y

Cuts at:      0     190     374     777 
   Size:        190     184     403

  Fragments arranged by size: 

                 403     190     184
 
AseI AT'TA_AT

Cuts at:      0     707     777 
   Size:        707      70
 
BanI G'GyrC_C

Cuts at:      0     351     777 
   Size:        351     426
 
BanII G_rGCy'C

Cuts at:      0     639     777 
   Size:        639     138
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     550     777 
   Size:        550     227
 
BglII A'GATC_T

Cuts at:      0      36     777 
   Size:         36     741
 
BlpI GC'TnA_GC

Cuts at:      0     462     777 
   Size:        462     315
 
Bme1580I G_kGCm'C

Cuts at:      0     294     574     777 
   Size:        294     280     203

  Fragments arranged by size: 

                 294     280     203
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     683     777 
   Size:        683      94
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     758     777 
   Size:        758      19
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     623     777 
   Size:        623     154
 
BsaAI yAC'GTr

Cuts at:      0     106     777 
   Size:        106     671
 
BsaBI GATnn'nnATC

Cuts at:      0     424     777 
   Size:        424     353
 
BsaWI w'CCGG_w

Cuts at:      0     392     777 
   Size:        392     385
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     604     777 
   Size:        604     173
 
BseYI C'CCAG_C

Cuts at:      0     654     777 
   Size:        654     123
 
BsiHKAI G_wGCw'C

Cuts at:      0     414     777 
   Size:        414     363
 
BsmI GAATG_Cn'

Cuts at:      0     388     777 
   Size:        388     389
 
Bsp1286I G_dGCh'C

Cuts at:      0     294     414     574     639     777 
   Size:        294     120     160      65     138

  Fragments arranged by size: 

                 294     160     138     120      65
 
BspHI T'CATG_A

Cuts at:      0     599     777 
   Size:        599     178
 
BsrBI CCG'CTC

Cuts at:      0     252     777 
   Size:        252     525
 
BsrDI GCAATG_nn'

Cuts at:      0     562     777 
   Size:        562     215
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      13     777 
   Size:         13     764
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     528     777 
   Size:        528     249
 
BstYI r'GATC_y

Cuts at:      0      36     777 
   Size:         36     741
 
Bsu36I CC'TnA_GG

Cuts at:      0     587     777 
   Size:        587     190
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     224     777 
   Size:        224     553
 
EcoO109I rG'GnC_Cy

Cuts at:      0     472     777 
   Size:        472     305
 
EcoRV GAT'ATC

Cuts at:      0     198     777 
   Size:        198     579
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     567     777 
   Size:        567     210
 
MslI CAynn'nnrTG

Cuts at:      0     526     777 
   Size:        526     251
 
MspA1I CmG'CkG

Cuts at:      0      70     777 
   Size:         70     707
 
NspI r_CATG'y

Cuts at:      0      11     654     777 
   Size:         11     643     123

  Fragments arranged by size: 

                 643     123      11
 
PasI CC'CwG_GG

Cuts at:      0     468     777 
   Size:        468     309
 
PfoI T'CCnGG_A

Cuts at:      0     401     777 
   Size:        401     376
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     532     564     777 
   Size:        532      32     213

  Fragments arranged by size: 

                 532     213      32
 
PstI C_TGCA'G

Cuts at:      0     234     767     777 
   Size:        234     533      10

  Fragments arranged by size: 

                 533     234      10
 
PvuII CAG'CTG

Cuts at:      0      70     777 
   Size:         70     707
 
SfcI C'TryA_G

Cuts at:      0     230     763     777 
   Size:        230     533      14

  Fragments arranged by size: 

                 533     230      14
 
SmlI C'TyrA_G

Cuts at:      0     638     777 
   Size:        638     139
 
SphI G_CATG'C

Cuts at:      0      11     654     777 
   Size:         11     643     123

  Fragments arranged by size: 

                 643     123      11
 
SspI AAT'ATT

Cuts at:      0     215     777 
   Size:        215     562
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     470     502     777 
   Size:        470      32     275

  Fragments arranged by size: 

                 470     275      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     362     547     777 
   Size:        362     185     230

  Fragments arranged by size: 

                 362     230     185
 

 Enzymes that do cut:

     AcuI     AleI     ApoI     AseI     BanI    BanII     BbsI    BglII
     BlpI Bme1580I     BmrI     BplI    BpuEI    BsaAI    BsaBI    BsaWI
    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I    BspHI    BsrBI    BsrDI
   BstAPI    BstXI    BstYI   Bsu36I  Eco57MI EcoO109I    EcoRV     FalI
     MslI   MspA1I     NspI     PasI     PfoI     PpiI     PstI    PvuII
     SfcI     SmlI     SphI     SspI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BmgBI     BmtI     BpmI   Bpu10I
     BsaI    BsaHI    BsaXI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BspMI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI
    PflMI     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     StuI     StyI     SwaI    TaqII     TatI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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