Restriction map for pF1KB7742
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       BamHI       
BbeI        BbrPI       BcgI        BciVI       BclI        
BfrI        BglII       BlpI        Bpu1102I    BsaAI       
BsaBI       BsiEI       BsiWI       BsmI        Bsp1407I    
BspDI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstZ17I     CelII       
ClaI        CpoI        DraI        DrdI        EagI        
Eam1105I    EclXI       Eco52I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MseI        MunI        MvnI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tru9I       Tth111I     Van91I      XbaI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccIII       1350  TCCGGA               T^CCGGA
AcsI          721  AAATTT               [GA]^AATT[CT]
AcyI          675  GACGCC               G[GA]^CG[CT]C
AocI          826  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       622  AGCGCT               AGC^GCT
ApaI          226  GGGCCC               GGGCC^C
ApoI          721  AAATTT               [GA]^AATT[CT]
AspHI        1198  GTGCTC               G[AT]GC[AT]^C
AvaII         606  GGACC                G^G[AT]CC
AvrII        1036  CCTAGG               C^CTAGG
BanI          700  GGTGCC               G^G[CT][GA]CC
BbsI          669  GAAGAC               GAAGAC(2/6)
BglI          984  GCCCATTAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         1036  CCTAGG               C^CTAGG
BpuAI         669  GAAGAC               GAAGAC(2/6)
BsaHI         675  GACGCC               G[GA]^CG[CT]C
BsaWI        1350  TCCGGA               [AT]^CCGG[AT]
BseAI        1350  TCCGGA               T^CCGGA
BsgI          290  GTGCAG               GTGCAG(16/14)
BsiHKAI      1198  GTGCTC               G[AT]GC[AT]^C
BsmBI         538  GAGACG               CGTCTC(1/5)
BspEI        1350  TCCGGA               T^CCGGA
BspMI         777  GCAGGT               ACCTGC(4/8)
BstXI         240  CCATGGCTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        826  CCTGAGG              CC^T[ACGT]AGG
Cac8I         861  GCCAGC               GC[ACGT]^[ACGT]GC
CfoI          623  GCGC                 GCG^C
DraIII        967  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III      622  AGCGCT               AGC^GCT
Eco81I        826  CCTGAGG              CC^T[ACGT]AGG
FauI         1398  GCGGG                CCCGC(4/6)
HaeII         622  AGCGCT               [GA]GCGC^[CT]
HhaI          623  GCGC                 GCG^C
Hin1I         675  GACGCC               G[GA]^CG[CT]C
HinP1I        623  GCGC                 G^CGC
HindIII      1447  AAGCTT               A^AGCTT
HphI           52  GGTGA                GGTGA(8/7)
MaeIII        811  GTGAC                ^GT[ACGT]AC
MroI         1350  TCCGGA               T^CCGGA
PaeR7I       1201  CTCGAG               C^TCGAG
SfcI           11  CTGTAG               C^T[GA][CT]AG
SmlI         1201  CTCGAG               C^T[CT][GA]AG
Tsp45I        811  GTGAC                ^GT[GC]AC
XcmI         1410  CCATGGCCACTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1201  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AciI          689  CCGC                 CCGC(-3/-1)
             1398  GCGG                
BbvCI         168  GCTGAGG              CCTCAGC(-5/-2)
              736  GCTGAGG             
BcnI         1002  CCCGG                CC^[GC]GG
             1254  CCCGG               
BfaI          695  CTAG                 C^TAG
             1037  CTAG                
BsaI           43  GAGACC               GGTCTC(1/5)
              272  GAGACC              
BsrFI         476  GCCGGT               [GA]^CCGG[CT]
              870  ACCGGC              
BssSI         550  CACGAG               CACGAG(-5/-1)
              461  CTCGTG              
BstYI          69  AGATCC               [GA]^GATC[CT]
             1289  GGATCT              
Cfr10I        476  GCCGGT               [GA]^CCGG[CT]
              870  ACCGGC              
DraII         225  AGGGCCC              [GA]G^G[ACGT]CC[CT]
              647  AGGCCCC             
EcoO109I      225  AGGGCCC              [GA]G^G[ACGT]CC[CT]
              647  AGGCCCC             
HgaI          140  GACGC                GACGC(5/10)
              675  GACGC               
MaeI          695  CTAG                 C^TAG
             1037  CTAG                
MflI           69  AGATCC               [GA]^GATC[CT]
             1289  GGATCT              
MspA1I        166  CAGCTG               C[AC]G^C[GT]G
             1295  CAGCTG              
NciI         1002  CCCGG                CC^[GC]GG
             1254  CCCGG               
PvuII         166  CAGCTG               CAG^CTG
             1295  CAGCTG              
SapI          856  GAAGAGC              GCTCTTC(1/4)
             1066  GAAGAGC             
SfaNI         343  GATGC                GCATC(5/9)
              235  GCATC               
StuI          902  AGGCCT               AGG^CCT
             1050  AGGCCT              
TaqI          128  TCGA                 T^CGA
             1202  TCGA                
TfiI          596  GAATC                G^A[AT]TC
             1213  GATTC               
Tsp509I       722  AATT                 ^AATT
             1438  AATT                
TthHB8I       128  TCGA                 T^CGA
             1202  TCGA                
XhoII          69  AGATCC               [GA]^GATC[CT]
             1289  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AfaI        AluI        AlwI        AlwNI       AvaI        
BalI        BanII       BbvI        BmyI        BpmI        
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cfr13I      DdeI        DpnII       
DsaI        EaeI        EarI        EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       ItaI        Ksp632I     MaeII       MboI        
MboII       MluNI       MnlI        MscI        MslI        
MspI        MvaI        MwoI        NcoI        NdeII       
NlaIII      NlaIV       PleI        RsaI        Sau3AI      
Sau96I      ScrFI       StyI        TseI        TspRI       
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