Restriction map for pF1KB7742
 (Linear) (Six-base) MAPSORT of: KIBB7742.seq  Check: 4908  from: 1  to: 1458



KIBB7742.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     768    1458 
   Size:        768     690
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     733    1229    1458 
   Size:        733     496     229

  Fragments arranged by size: 

                 733     496     229
 
AfeI AGC'GCT

Cuts at:      0     624    1458 
   Size:        624     834
 
AlwNI CAG_nnn'CTG

Cuts at:      0      73     940    1090    1458 
   Size:         73     867     150     368

  Fragments arranged by size: 

                 867     368     150      73
 
ApaI G_GGCC'C

Cuts at:      0     230    1458 
   Size:        230    1228
 
ApoI r'AATT_y

Cuts at:      0     721    1458 
   Size:        721     737
 
AvaI C'yCGr_G

Cuts at:      0    1102    1114    1201    1458 
   Size:       1102      12      87     257

  Fragments arranged by size: 

                1102     257      87      12
 
AvrII C'CTAG_G

Cuts at:      0    1036    1458 
   Size:       1036     422
 
BanI G'GyrC_C

Cuts at:      0     700    1458 
   Size:        700     758
 
BanII G_rGCy'C

Cuts at:      0     230     826    1022    1103    1115    1458 
   Size:        230     596     196      81      12     343

  Fragments arranged by size: 

                 596     343     230     196      81      12
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     676    1458 
   Size:        676     782
 
BbvCI CC'TCA_GC

Cuts at:      0     169     737    1458 
   Size:        169     568     721

  Fragments arranged by size: 

                 721     568     169
 
BglI GCCn_nnn'nGGC

Cuts at:      0     990    1458 
   Size:        990     468
 
Bme1580I G_kGCm'C

Cuts at:      0     230     705     977    1458 
   Size:        230     475     272     481

  Fragments arranged by size: 

                 481     475     272     230
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     964    1458 
   Size:        964     494
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     345    1458 
   Size:        345    1113
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     896    1039    1458 
   Size:        896     143     419

  Fragments arranged by size: 

                 896     419     143
 
Bpu10I CC'TnA_GC

Cuts at:      0     169     737    1091    1097    1109    1458 
   Size:        169     568     354       6      12     349

  Fragments arranged by size: 

                 568     354     349     169      12       6
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      37     266    1458 
   Size:         37     229    1192

  Fragments arranged by size: 

                1192     229      37
 
BsaHI Gr'CG_yC

Cuts at:      0     676    1458 
   Size:        676     782
 
BsaWI w'CCGG_w

Cuts at:      0    1350    1458 
   Size:       1350     108
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     202     754     778     834     885     914    1030    1159 
   Size:        202     552      24      56      51      29     116     129

Cuts at:   1159    1207    1458 
   Size:         48     251

  Fragments arranged by size: 

                 552     251     202     129     116      56      51      48
                  29      24
 
BseYI C'CCAG_C

Cuts at:      0    1023    1258    1264    1270    1458 
   Size:       1023     235       6       6     188

  Fragments arranged by size: 

                1023     235     188       6       6
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     311    1458 
   Size:        311    1147
 
BsiHKAI G_wGCw'C

Cuts at:      0    1202    1458 
   Size:       1202     256
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     532    1458 
   Size:        532     926
 
Bsp1286I G_dGCh'C

Cuts at:      0     230     705     826     977    1022    1103    1115    1202 
   Size:        230     475     121     151      45      81      12      87

Cuts at:   1202    1458 
   Size:        256

  Fragments arranged by size: 

                 475     256     230     151     121      87      81      45
                  12
 
BspEI T'CCGG_A

Cuts at:      0    1350    1458 
   Size:       1350     108
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     768    1458 
   Size:        768     690
 
BsrFI r'CCGG_y

Cuts at:      0     476     870    1458 
   Size:        476     394     588

  Fragments arranged by size: 

                 588     476     394
 
BssSI C'ACGA_G

Cuts at:      0     461     550    1458 
   Size:        461      89     908

  Fragments arranged by size: 

                 908     461      89
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     247    1458 
   Size:        247    1211
 
BstYI r'GATC_y

Cuts at:      0      69    1289    1458 
   Size:         69    1220     169

  Fragments arranged by size: 

                1220     169      69
 
Bsu36I CC'TnA_GG

Cuts at:      0     827    1458 
   Size:        827     631
 
BtgI C'CryG_G

Cuts at:      0      38     240     705     731    1410    1458 
   Size:         38     202     465      26     679      48

  Fragments arranged by size: 

                 679     465     202      48      38      26
 
BtsI GCAGTG_nn'

Cuts at:      0     299     877    1458 
   Size:        299     578     581

  Fragments arranged by size: 

                 581     578     299
 
DraIII CAC_nnn'GTG

Cuts at:      0     972    1458 
   Size:        972     486
 
EaeI y'GGCC_r

Cuts at:      0     474     585     782    1413    1458 
   Size:        474     111     197     631      45

  Fragments arranged by size: 

                 631     474     197     111      45
 
EarI CTCTTCn'nnn_

Cuts at:      0     336     739     755     851    1061    1432    1458 
   Size:        336     403      16      96     210     371      26

  Fragments arranged by size: 

                 403     371     336     210      96      26      16
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     733     896    1039    1229    1458 
   Size:        733     163     143     190     229

  Fragments arranged by size: 

                 733     229     190     163     143
 
EcoO109I rG'GnC_Cy

Cuts at:      0     226     648    1458 
   Size:        226     422     810

  Fragments arranged by size: 

                 810     422     226
 
HaeII r_GCGC'y

Cuts at:      0     626    1458 
   Size:        626     832
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     205     237    1458 
   Size:        205      32    1221

  Fragments arranged by size: 

                1221     205      32
 
HindIII A'AGCT_T

Cuts at:      0    1447    1458 
   Size:       1447      11
 
MscI TGG'CCA

Cuts at:      0     587     784    1415    1458 
   Size:        587     197     631      43

  Fragments arranged by size: 

                 631     587     197      43
 
MslI CAynn'nnrTG

Cuts at:      0     245     305    1375    1458 
   Size:        245      60    1070      83

  Fragments arranged by size: 

                1070     245      83      60
 
MspA1I CmG'CkG

Cuts at:      0     168    1297    1458 
   Size:        168    1129     161

  Fragments arranged by size: 

                1129     168     161
 
NcoI C'CATG_G

Cuts at:      0     240     731    1410    1458 
   Size:        240     491     679      48

  Fragments arranged by size: 

                 679     491     240      48
 
PasI CC'CwG_GG

Cuts at:      0      48    1458 
   Size:         48    1410
 
PfoI T'CCnGG_A

Cuts at:      0    1001    1458 
   Size:       1001     457
 
PsiI TTA'TAA

Cuts at:      0     419     665    1458 
   Size:        419     246     793

  Fragments arranged by size: 

                 793     419     246
 
PspOMI G'GGCC_C

Cuts at:      0     226    1458 
   Size:        226    1232
 
PspXI vC'TCGA_Gb

Cuts at:      0    1201    1458 
   Size:       1201     257
 
PvuII CAG'CTG

Cuts at:      0     168    1297    1458 
   Size:        168    1129     161

  Fragments arranged by size: 

                1129     168     161
 
SapI GCTCTTCn'nnn_

Cuts at:      0     851    1061    1458 
   Size:        851     210     397

  Fragments arranged by size: 

                 851     397     210
 
SfcI C'TryA_G

Cuts at:      0      11    1458 
   Size:         11    1447
 
SmlI C'TyrA_G

Cuts at:      0    1201    1458 
   Size:       1201     257
 
StuI AGG'CCT

Cuts at:      0     904    1052    1458 
   Size:        904     148     406

  Fragments arranged by size: 

                 904     406     148
 
StyI C'CwwG_G

Cuts at:      0     240     572     731    1036    1317    1410    1458 
   Size:        240     332     159     305     281      93      48

  Fragments arranged by size: 

                 332     305     281     240     159      93      48
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     290     623     983    1458 
   Size:        290     333     360     475

  Fragments arranged by size: 

                 475     360     333     290
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1417    1458 
   Size:       1417      41
 
XhoI C'TCGA_G

Cuts at:      0    1201    1458 
   Size:       1201     257
 

 Enzymes that do cut:

     AarI     AcuI     AfeI    AlwNI     ApaI     ApoI     AvaI    AvrII
     BanI    BanII     BbsI    BbvCI     BglI Bme1580I     BmrI     BplI
     BpmI   Bpu10I     BsaI    BsaHI    BsaWI    BseRI    BseYI     BsgI
  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI    BsrFI    BssSI    BstXI
    BstYI   Bsu36I     BtgI     BtsI   DraIII     EaeI     EarI  Eco57MI
 EcoO109I    HaeII    Hin4I  HindIII     MscI     MslI   MspA1I     NcoI
     PasI     PfoI     PsiI   PspOMI    PspXI    PvuII     SapI     SfcI
     SmlI     StuI     StyI    TaqII     XcmI     XhoI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI     BaeI
    BamHI     BbeI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
    BmgBI     BmtI    BpuEI    BsaAI    BsaBI    BsaXI    BsiEI    BsiWI
     BsmI    BspHI    BsrBI    BsrDI    BsrGI   BssHII   BstAPI    BstBI
   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI     DrdI     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI   HincII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI
     TstI  Tth111I     XbaI     XmaI     XmnI     ZraI
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