Restriction map for pF1KB7788
##################################
# NO CUT ENZYMES (  149 enzymes) #
##################################

AatII       Acc65I      AccIII      AciI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbvCI       BcgI        BclI        BcnI        
BfrI        BglI        BlnI        BlpI        BpmI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiWI       BsmAI       BsmBI       BsoBI       BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BssHII      BssSI       Bst1107I    
BstEII      BstPI       BstXI       BstZ17I     CelII       
CfoI        Cfr10I      ClaI        CpoI        DraIII      
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HapII       HhaI        Hin1I       
HinP1I      HpaI        HpaII       KasI        KpnI        
KspI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MspI        MunI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NsiI        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SfiI        SfoI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        SwaI        Tth111I     Van91I      
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccI         1001  GTCTAC               GT^[AC][GT]AC
AccII         351  CGCG                 CG^CG
AclI         1484  AACGTT               AA^CGTT
AcsI          117  GAATTT               [GA]^AATT[CT]
AocI           74  CCTCAGG              CC^T[ACGT]AGG
ApoI          117  GAATTT               [GA]^AATT[CT]
AseI         1027  ATTAAT               AT^TAAT
AsnI         1027  ATTAAT               AT^TAAT
AspHI         381  GTGCTC               G[AT]GC[AT]^C
BbsI           15  GAAGAC               GAAGAC(2/6)
BbvI          102  GCTGC                GCAGC(8/12)
BciVI        1365  GTATCC               GTATCC(6/5)
BmyI          381  GTGCTC               G[AGT]GC[ACT]^C
BpuAI          15  GAAGAC               GAAGAC(2/6)
BsiHKAI       381  GTGCTC               G[AT]GC[AT]^C
BsiYI         504  CCATTGGAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          504  CCATTGGAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI        1270  GGGAC                GGGAC(10/14)
Bsp1286I      381  GTGCTC               G[AGT]GC[ACT]^C
BstAPI        563  GCATTTTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         474  TTCGAA               TT^CGAA
BstF5I        604  GGATG                GGATG(2/0)
BstUI         351  CGCG                 CG^CG
Bsu36I         74  CCTCAGG              CC^T[ACGT]AGG
DraII        1152  AGGACCT              [GA]G^G[ACGT]CC[CT]
DrdI         1154  GACCTGGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I         74  CCTCAGG              CC^T[ACGT]AGG
EcoO109I     1152  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoT14I      1401  CCTTGG               C^C[AT][AT]GG
Fnu4HI        102  GCTGC                GC^[ACGT]GC
FokI          604  GGATG                GGATG(9/13)
HaeIII        620  GGCC                 GG^CC
HgaI         1128  GCGTC                GACGC(5/10)
HincII        837  GTTGAC               GT[CT]^[GA]AC
HindII        837  GTTGAC               GT[CT]^[GA]AC
HindIII       942  AAGCTT               A^AGCTT
ItaI          102  GCTGC                GC^[ACGT]GC
MaeII        1485  ACGT                 A^CGT
MaeIII       1406  GTGAC                ^GT[ACGT]AC
MspA1I        226  CAGCTG               C[AC]G^C[GT]G
MvnI          351  CGCG                 CG^CG
NlaIV         272  GGAACC               GG[ACGT]^[ACGT]CC
NruI          350  TCGCGA               TCG^CGA
NspV          474  TTCGAA               TT^CGAA
PpuMI        1152  AGGACCT              [GA]G^G[AT]CC[CT]
PshBI        1027  ATTAAT               AT^TAAT
Psp1406I     1484  AACGTT               AA^CGTT
PvuII         226  CAGCTG               CAG^CTG
SexAI        1155  ACCTGGT              A^CC[AT]GGT
SfuI          474  TTCGAA               TT^CGAA
StyI         1401  CCTTGG               C^C[AT][AT]GG
TaqI          475  TCGA                 T^CGA
TseI          102  GCTGC                G^C[AT]GC
Tsp45I       1406  GTGAC                ^GT[GC]AC
TthHB8I       475  TCGA                 T^CGA
XcmI          259  CCAAGATCATCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AlwNI         554  CAGAATCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1268  CAGGGACTG           
AvaII         894  GGACC                G^G[AT]CC
             1153  GGACC               
BfaI          425  CTAG                 C^TAG
              713  CTAG                
BglII         741  AGATCT               A^GATCT
             1177  AGATCT              
BsaJI        1369  CCAGGG               C^C[ACGT][ACGT]GG
             1401  CCTTGG              
Bsp1407I      408  TGTACA               T^GTACA
             1445  TGTACA              
BsrGI         408  TGTACA               T^GTACA
             1445  TGTACA              
BstYI         741  AGATCT               [GA]^GATC[CT]
             1177  AGATCT              
Cfr13I        894  GGACC                G^G[ACGT]CC
             1153  GGACC               
DraI         1060  TTTAAA               TTT^AAA
             1488  TTTAAA              
MaeI          425  CTAG                 C^TAG
              713  CTAG                
MflI          741  AGATCT               [GA]^GATC[CT]
             1177  AGATCT              
MwoI          343  GCAAATATCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              563  GCATTTTCTGC         
NspI          218  GCATGT               [GA]CATG^[CT]
              572  GCATGT              
PstI          103  CTGCAG               CTGCA^G
             1094  CTGCAG              
Sau96I        894  GGACC                G^G[ACGT]CC
             1153  GGACC               
SfcI          103  CTGCAG               C^T[GA][CT]AG
             1094  CTGCAG              
SspBI         408  TGTACA               T^GTACA
             1445  TGTACA              
XhoII         741  AGATCT               [GA]^GATC[CT]
             1177  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AfaI        AluI        AlwI        Asp700I     BsmI        
BsrI        BssKI       BstNI       Cac8I       DdeI        
DpnII       EarI        EcoRII      HinfI       HphI        
Ksp632I     MboI        MboII       MnlI        MseI        
MslI        MvaI        NdeII       NlaIII      PleI        
RsaI        Sau3AI      ScrFI       SfaNI       TfiI        
Tru9I       Tsp509I     TspRI       XmnI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com