Restriction map for pF1KB7788
 (Linear) (Six-base) MAPSORT of: KIBB7788.seq  Check: 2772  from: 1  to: 1542



KIBB7788.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

AccI GT'mk_AC

Cuts at:      0    1002    1542 
   Size:       1002     540
 
AclI AA'CG_TT

Cuts at:      0    1485    1542 
   Size:       1485      57
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     740     853    1493    1542 
   Size:        169     571     113     640      49

  Fragments arranged by size: 

                 640     571     169     113      49
 
AleI CACnn'nnGTG

Cuts at:      0     660    1542 
   Size:        660     882
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     476     508    1542 
   Size:        476      32    1034

  Fragments arranged by size: 

                1034     476      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     559    1273    1542 
   Size:        559     714     269

  Fragments arranged by size: 

                 714     559     269
 
ApoI r'AATT_y

Cuts at:      0     117    1542 
   Size:        117    1425
 
AseI AT'TA_AT

Cuts at:      0    1028    1542 
   Size:       1028     514
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      22    1542 
   Size:         22    1520
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1376    1542 
   Size:       1376     166
 
BglII A'GATC_T

Cuts at:      0     741    1177    1542 
   Size:        741     436     365

  Fragments arranged by size: 

                 741     436     365
 
Bpu10I CC'TnA_GC

Cuts at:      0    1266    1542 
   Size:       1266     276
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      32      62    1542 
   Size:         32      30    1480

  Fragments arranged by size: 

                1480      32      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     385    1542 
   Size:        385    1157
 
BsmI GAATG_Cn'

Cuts at:      0     755     965    1320    1542 
   Size:        755     210     355     222

  Fragments arranged by size: 

                 755     355     222     210
 
Bsp1286I G_dGCh'C

Cuts at:      0     385    1542 
   Size:        385    1157
 
BsrGI T'GTAC_A

Cuts at:      0     408    1445    1542 
   Size:        408    1037      97

  Fragments arranged by size: 

                1037     408      97
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     569    1542 
   Size:        569     973
 
BstBI TT'CG_AA

Cuts at:      0     475    1542 
   Size:        475    1067
 
BstYI r'GATC_y

Cuts at:      0     741    1177    1542 
   Size:        741     436     365

  Fragments arranged by size: 

                 741     436     365
 
Bsu36I CC'TnA_GG

Cuts at:      0      75    1542 
   Size:         75    1467
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1007    1542 
   Size:       1007     535
 
BtsI GCAGTG_nn'

Cuts at:      0     112     385    1542 
   Size:        112     273    1157

  Fragments arranged by size: 

                1157     273     112
 
DraI TTT'AAA

Cuts at:      0    1062    1490    1542 
   Size:       1062     428      52

  Fragments arranged by size: 

                1062     428      52
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1160    1542 
   Size:       1160     382
 
EarI CTCTTCn'nnn_

Cuts at:      0     472     474    1230    1427    1501    1542 
   Size:        472       2     756     197      74      41

  Fragments arranged by size: 

                 756     472     197      74      41       2
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     740     853    1493    1542 
   Size:        169     571     113     640      49

  Fragments arranged by size: 

                 640     571     169     113      49
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1153    1542 
   Size:       1153     389
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     284    1542 
   Size:        284    1258
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     541     573     831     863    1542 
   Size:        541      32     258      32     679

  Fragments arranged by size: 

                 679     541     258      32      32
 
HincII GTy'rAC

Cuts at:      0     839    1542 
   Size:        839     703
 
HindIII A'AGCT_T

Cuts at:      0     942    1542 
   Size:        942     600
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1483    1542 
   Size:       1483      59
 
MslI CAynn'nnrTG

Cuts at:      0     443     660    1019    1081    1393    1542 
   Size:        443     217     359      62     312     149

  Fragments arranged by size: 

                 443     359     312     217     149      62
 
MspA1I CmG'CkG

Cuts at:      0     228    1542 
   Size:        228    1314
 
NruI TCG'CGA

Cuts at:      0     352    1542 
   Size:        352    1190
 
NspI r_CATG'y

Cuts at:      0     222     576    1542 
   Size:        222     354     966

  Fragments arranged by size: 

                 966     354     222
 
PfoI T'CCnGG_A

Cuts at:      0    1008    1542 
   Size:       1008     534
 
PpuMI rG'GwC_Cy

Cuts at:      0    1153    1542 
   Size:       1153     389
 
PstI C_TGCA'G

Cuts at:      0     107    1098    1542 
   Size:        107     991     444

  Fragments arranged by size: 

                 991     444     107
 
PvuII CAG'CTG

Cuts at:      0     228    1542 
   Size:        228    1314
 
SexAI A'CCwGG_T

Cuts at:      0    1155    1542 
   Size:       1155     387
 
SfcI C'TryA_G

Cuts at:      0     103    1094    1542 
   Size:        103     991     448

  Fragments arranged by size: 

                 991     448     103
 
StyI C'CwwG_G

Cuts at:      0    1401    1542 
   Size:       1401     141
 
TatI w'GTAC_w

Cuts at:      0      39     408     828    1445    1542 
   Size:         39     369     420     617      97

  Fragments arranged by size: 

                 617     420     369      97      39
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1263    1295    1542 
   Size:       1263      32     247

  Fragments arranged by size: 

                1263     247      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     266    1542 
   Size:        266    1276
 
XmnI GAAnn'nnTTC

Cuts at:      0     743    1318    1521    1542 
   Size:        743     575     203      21

  Fragments arranged by size: 

                 743     575     203      21
 

 Enzymes that do cut:

     AccI     AclI     AcuI     AleI     AloI    AlwNI     ApoI     AseI
     BbsI    BciVI    BglII   Bpu10I    BsaXI  BsiHKAI     BsmI Bsp1286I
    BsrGI   BstAPI    BstBI    BstYI   Bsu36I    BtgZI     BtsI     DraI
     DrdI     EarI  Eco57MI EcoO109I     FalI    Hin4I   HincII  HindIII
     MmeI     MslI   MspA1I     NruI     NspI     PfoI    PpuMI     PstI
    PvuII    SexAI     SfcI     StyI     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI    BanII     BbeI    BbvCI     BcgI     BclI    BfrBI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI
     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstEII    BstXI  BstZ17I     BtgI
     ClaI    CspCI   DraIII     EaeI     EagI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NsiI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII  Tth111I
     XbaI     XhoI     XmaI     ZraI
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