Restriction map for pF1KB7292
##################################
# NO CUT ENZYMES (   58 enzymes) #
##################################

AatII       Acc65I      AccIII      AcsI        AflII       
ApaI        ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AvrII       BamHI       BbvCI       
BfrI        BlnI        BseAI       BsiWI       BspDI       
BspEI       BspLU11I    BssHII      Bst1107I    BstBI       
BstZ17I     ClaI        CpoI        EcoRI       FseI        
HincII      HindII      HpaI        KpnI        MluI        
MroI        NaeI        NgoMIV      NotI        NruI        
NspV        PacI        PaeR7I      PmeI        PshBI       
PvuI        RsrII       SalI        ScaI        SfuI        
SgrAI       SnaBI       SpeI        SspI        SwaI        
XbaI        XhoI        XmnI        


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# ONE CUT ENZYMES (   63 enzymes) #
###################################

AccI         2224  GTCTAC               GT^[AC][GT]AC
AclI          659  AACGTT               AA^CGTT
AgeI          413  ACCGGT               A^CCGGT
AhdI          427  GACGGCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          603  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       104  AGCGCT               AGC^GCT
AspEI         427  GACGGCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         821  TGCGCA               TGC^GCA
BbeI          911  GGCGCC               GGCGC^C
BciVI        3529  GTATCC               GTATCC(6/5)
BglI         1301  GCCACGTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         3243  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     3243  GCTGAGC              GC^T[ACGT]AGC
BsaBI        2502  GATGTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         413  ACCGGT               [AT]^CCGG[AT]
BsiEI         173  CGGCCG               CG[GA][CT]^CG
BsmI         2652  GAATGC               GAATGC(1/-1)
BsrFI         413  ACCGGT               [GA]^CCGG[CT]
BstEII       2163  GGTAACC              G^GT[ACGT]ACC
BstPI        2163  GGTAACC              G^GT[ACGT]ACC
Bsu36I        603  CCTGAGG              CC^T[ACGT]AGG
CelII        3243  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        413  ACCGGT               [GA]^CCGG[CT]
DraI         1168  TTTAAA               TTT^AAA
DraIII       3145  CACGCGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          173  CGGCCG               C^GGCCG
Eam1105I      427  GACGGCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         173  CGGCCG               C^GGCCG
Eco47III      104  AGCGCT               AGC^GCT
Eco52I        173  CGGCCG               C^GGCCG
Eco81I        603  CCTGAGG              CC^T[ACGT]AGG
EcoNI        3024  CCTCCCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      2163  GGTAACC              G^GT[ACGT]ACC
EcoRV        1438  GATATC               GAT^ATC
EcoT22I      2572  ATGCAT               ATGCA^T
FspI          821  TGCGCA               TGC^GCA
KasI          911  GGCGCC               G^GCGCC
KspI         3278  CCGCGG               CCGC^GG
MamI         2502  GATGTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          666  CAATTG               C^AATTG
MunI          666  CAATTG               C^AATTG
NarI          911  GGCGCC               GG^CGCC
NdeI         2722  CATATG               CA^TATG
NheI         2394  GCTAGC               G^CTAGC
NsiI         2572  ATGCAT               ATGCA^T
PinAI         413  ACCGGT               A^CCGGT
PpuMI        3386  AGGACCT              [GA]G^G[AT]CC[CT]
PshAI         672  GACCTATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I      659  AACGTT               AA^CGTT
PvuII        3241  CAGCTG               CAG^CTG
SacI         1885  GAGCTC               GAGCT^C
SacII        3278  CCGCGG               CCGC^GG
SbfI         3390  CCTGCAGG             CCTGCA^GG
SexAI        2691  ACCAGGT              A^CC[AT]GGT
SfiI         1300  GGCCACGTGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          911  GGCGCC               GGC^GCC
SmaI         3172  CCCGGG               CCC^GGG
SmlI         1756  CTTGAG               C^T[CT][GA]AG
SphI         2570  GCATGC               GCATG^C
Sse8387I     3390  CCTGCAGG             CCTGCA^GG
StuI          753  AGGCCT               AGG^CCT
XmaCI        3172  CCCGGG               C^CCGGG
XmaI         3172  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AflIII        251  ACACGT               A^C[GA][CT]GT
             3326  ACGTGT              
AspI          274  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              649  GACATAGTC           
BalI          113  TGGCCA               TGG^CCA
             1299  TGGCCA              
BbrPI        1303  CACGTG               CAC^GTG
             2746  CACGTG              
BbsI         2751  GAAGAC               GAAGAC(2/6)
             3361  GAAGAC              
BcgI         1016  CGATAAAGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
               88  GCAGGAGCGTCG        
BclI         2120  TGATCA               T^GATCA
             2171  TGATCA              
BglII        1719  AGATCT               A^GATCT
             2075  AGATCT              
BpuAI        2751  GAAGAC               GAAGAC(2/6)
             3361  GAAGAC              
BsaI         2995  GAGACC               GGTCTC(1/5)
             3267  GGTCTC              
BssSI        1086  CACGAG               CACGAG(-5/-1)
             3337  CACGAG              
BstXI        2243  CCAGCAACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2811  CCAAGGCTCTGG        
DraII        3159  AGGCCCC              [GA]G^G[ACGT]CC[CT]
             3386  AGGACCT             
DrdI          124  GACGGAGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3435  GACGCCTCCGTC        
EcoO109I     3159  AGGCCCC              [GA]G^G[ACGT]CC[CT]
             3386  AGGACCT             
FbaI         2120  TGATCA               T^GATCA
             2171  TGATCA              
HindIII      1194  AAGCTT               A^AGCTT
             1688  AAGCTT              
MluNI         113  TGGCCA               TGG^CCA
             1299  TGGCCA              
MscI          113  TGGCCA               TGG^CCA
             1299  TGGCCA              
NcoI         1256  CCATGG               C^CATGG
             2924  CCATGG              
PflFI         274  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              649  GACATAGTC           
PmaCI        1303  CACGTG               CAC^GTG
             2746  CACGTG              
PmlI         1303  CACGTG               CAC^GTG
             2746  CACGTG              
Tth111I       274  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              649  GACATAGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  113 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
Alw44I      AlwI        AlwNI       ApaLI       AspHI       
AvaI        AvaII       BanI        BanII       BbvI        
BcnI        BfaI        BmyI        BpmI        BsaAI       
BsaHI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsoBI       Bsp1286I    Bsp1407I    BspHI       BspMI       
BsrBI       BsrDI       BsrGI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DsaI        EaeI        EarI        EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PstI        RcaI        RsaI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SspBI       StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XhoII       
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