Restriction map for pF1KB7292
 (Linear) (Six-base) MAPSORT of: KIBB7292.seq  Check: 9197  from: 1  to: 3582



KIBB7292.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1690    3230    3582 
   Size:       1690    1540     352

  Fragments arranged by size: 

                1690    1540     352
 
AccI GT'mk_AC

Cuts at:      0    2225    3582 
   Size:       2225    1357
 
AclI AA'CG_TT

Cuts at:      0     660    3582 
   Size:        660    2922
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1481    3105    3582 
   Size:       1481    1624     477

  Fragments arranged by size: 

                1624    1481     477
 
AfeI AGC'GCT

Cuts at:      0     106    3582 
   Size:        106    3476
 
AflIII A'CryG_T

Cuts at:      0     251    3326    3582 
   Size:        251    3075     256

  Fragments arranged by size: 

                3075     256     251
 
AgeI A'CCGG_T

Cuts at:      0     413    3582 
   Size:        413    3169
 
AhdI GACnn_n'nnGTC

Cuts at:      0     432    3582 
   Size:        432    3150
 
AleI CACnn'nnGTG

Cuts at:      0    1861    3582 
   Size:       1861    1721
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     901    1363    3496    3582 
   Size:        901     462    2133      86

  Fragments arranged by size: 

                2133     901     462      86
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     229     261    3008    3040    3582 
   Size:        229      32    2747      32     542

  Fragments arranged by size: 

                2747     542     229      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     519    3222    3231    3582 
   Size:        519    2703       9     351

  Fragments arranged by size: 

                2703     519     351       9
 
ApaLI G'TGCA_C

Cuts at:      0    1483    2743    2932    3582 
   Size:       1483    1260     189     650

  Fragments arranged by size: 

                1483    1260     650     189
 
AvaI C'yCGr_G

Cuts at:      0     187    3172    3468    3582 
   Size:        187    2985     296     114

  Fragments arranged by size: 

                2985     296     187     114
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2196    2229    3108    3141    3582 
   Size:       2196      33     879      33     441

  Fragments arranged by size: 

                2196     879     441      33      33
 
BanI G'GyrC_C

Cuts at:      0     911    2706    2729    3480    3582 
   Size:        911    1795      23     751     102

  Fragments arranged by size: 

                1795     911     751     102      23
 
BanII G_rGCy'C

Cuts at:      0      11     147    1739    1889    2115    3582 
   Size:         11     136    1592     150     226    1467

  Fragments arranged by size: 

                1592    1467     226     150     136      11
 
BbeI G_GCGC'C

Cuts at:      0     915    3582 
   Size:        915    2667
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2758    3368    3582 
   Size:       2758     610     214

  Fragments arranged by size: 

                2758     610     214
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      77     111    1005    1039    3582 
   Size:         77      34     894      34    2543

  Fragments arranged by size: 

                2543     894      77      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    3540    3582 
   Size:       3540      42
 
BclI T'GATC_A

Cuts at:      0    2120    2171    3582 
   Size:       2120      51    1411

  Fragments arranged by size: 

                2120    1411      51
 
BfrBI ATG'CAT

Cuts at:      0    2574    3582 
   Size:       2574    1008
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1307    3582 
   Size:       1307    2275
 
BglII A'GATC_T

Cuts at:      0    1719    2075    3582 
   Size:       1719     356    1507

  Fragments arranged by size: 

                1719    1507     356
 
BlpI GC'TnA_GC

Cuts at:      0    3244    3582 
   Size:       3244     338
 
Bme1580I G_kGCm'C

Cuts at:      0     188    1487    1933    2333    2408    2711    2747    2936 
   Size:        188    1299     446     400      75     303      36     189

Cuts at:   2936    3485    3582 
   Size:        549      97

  Fragments arranged by size: 

                1299     549     446     400     303     189     188      97
                  75      36
 
BmtI G_CTAG'C

Cuts at:      0    2398    3582 
   Size:       2398    1184
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3285    3582 
   Size:       3285     297
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     474     508    1016    1409    1860    1960    3034    3085 
   Size:        474      34     508     393     451     100    1074      51

Cuts at:   3085    3301    3582 
   Size:        216     281

  Fragments arranged by size: 

                1074     508     474     451     393     281     216     100
                  51      34
 
Bpu10I CC'TnA_GC

Cuts at:      0     898    2114    3582 
   Size:        898    1216    1468

  Fragments arranged by size: 

                1468    1216     898
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1777    3582 
   Size:       1777    1805
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2989    3273    3582 
   Size:       2989     284     309

  Fragments arranged by size: 

                2989     309     284
 
BsaAI yAC'GTr

Cuts at:      0    1305    2748    3327    3582 
   Size:       1305    1443     579     255

  Fragments arranged by size: 

                1443    1305     579     255
 
BsaBI GATnn'nnATC

Cuts at:      0    2506    3582 
   Size:       2506    1076
 
BsaHI Gr'CG_yC

Cuts at:      0     912     920    3436    3582 
   Size:        912       8    2516     146

  Fragments arranged by size: 

                2516     912     146       8
 
BsaWI w'CCGG_w

Cuts at:      0     413    3582 
   Size:        413    3169
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     229     259    1382    1387    1412    1417    2690    2720 
   Size:        229      30    1123       5      25       5    1273      30

Cuts at:   2720    3008    3038    3313    3343    3582 
   Size:        288      30     275      30     239

  Fragments arranged by size: 

                1273    1123     288     275     239     229      30      30
                  30      30      25       5       5
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1408    1989    2275    2887    2890    2893    3007    3035 
   Size:       1408     581     286     612       3       3     114      28

Cuts at:   3035    3274    3304    3391    3394    3397    3582 
   Size:        239      30      87       3       3     185

  Fragments arranged by size: 

                1408     612     581     286     239     185     114      87
                  30      28       3       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0      27     793    2888    3484    3582 
   Size:         27     766    2095     596      98

  Fragments arranged by size: 

                2095     766     596      98      27
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     532    1164    1590    2064    3498    3582 
   Size:        532     632     426     474    1434      84

  Fragments arranged by size: 

                1434     632     532     474     426      84
 
BsiEI CG_ry'CG

Cuts at:      0     176    3582 
   Size:        176    3406
 
BsiHKAI G_wGCw'C

Cuts at:      0    1487    1889    2747    2936    3250    3338    3512    3582 
   Size:       1487     402     858     189     314      88     174      70

  Fragments arranged by size: 

                1487     858     402     314     189     174      88      70
            
 
BsmI GAATG_Cn'

Cuts at:      0    2658    3582 
   Size:       2658     924
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     104    1412    2755    3582 
   Size:        104    1308    1343     827

  Fragments arranged by size: 

                1343    1308     827     104
 
Bsp1286I G_dGCh'C

Cuts at:      0      11     147     188    1487    1739    1889    1933    2115 
   Size:         11     136      41    1299     252     150      44     182

Cuts at:   2115    2333    2408    2711    2747    2936    3250    3338    3485 
   Size:        218      75     303      36     189     314      88     147

Cuts at:   3485    3512    3582 
   Size:         27      70

  Fragments arranged by size: 

                1299     314     303     252     218     189     182     150
                 147     136      88      75      70      44      41      36
                  27      11
 
BspHI T'CATG_A

Cuts at:      0    1442    2099    2291    3582 
   Size:       1442     657     192    1291

  Fragments arranged by size: 

                1442    1291     657     192
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     527    1690    3230    3398    3582 
   Size:        527    1163    1540     168     184

  Fragments arranged by size: 

                1540    1163     527     184     168
 
BsrBI CCG'CTC

Cuts at:      0     716    2603    3342    3582 
   Size:        716    1887     739     240

  Fragments arranged by size: 

                1887     739     716     240
 
BsrDI GCAATG_nn'

Cuts at:      0     464    1194    1943    2123    3582 
   Size:        464     730     749     180    1459

  Fragments arranged by size: 

                1459     749     730     464     180
 
BsrFI r'CCGG_y

Cuts at:      0     413    3582 
   Size:        413    3169
 
BsrGI T'GTAC_A

Cuts at:      0     590    2372    2504    3582 
   Size:        590    1782     132    1078

  Fragments arranged by size: 

                1782    1078     590     132
 
BssSI C'ACGA_G

Cuts at:      0    1086    3337    3582 
   Size:       1086    2251     245

  Fragments arranged by size: 

                2251    1086     245
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     519    1278    1646    2653    3222    3231    3582 
   Size:        519     759     368    1007     569       9     351

  Fragments arranged by size: 

                1007     759     569     519     368     351       9
 
BstEII G'GTnAC_C

Cuts at:      0    2163    3582 
   Size:       2163    1419
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2250    2818    3582 
   Size:       2250     568     764

  Fragments arranged by size: 

                2250     764     568
 
BstYI r'GATC_y

Cuts at:      0     311    1719    2075    3582 
   Size:        311    1408     356    1507

  Fragments arranged by size: 

                1507    1408     356     311
 
Bsu36I CC'TnA_GG

Cuts at:      0     604    3582 
   Size:        604    2978
 
BtgI C'CryG_G

Cuts at:      0     338    1256    2924    3278    3582 
   Size:        338     918    1668     354     304

  Fragments arranged by size: 

                1668     918     354     338     304
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1331    1773    2020    2597    3582 
   Size:       1331     442     247     577     985

  Fragments arranged by size: 

                1331     985     577     442     247
 
BtsI GCAGTG_nn'

Cuts at:      0     312    1578    2459    2547    3582 
   Size:        312    1266     881      88    1035

  Fragments arranged by size: 

                1266    1035     881     312      88
 
DraI TTT'AAA

Cuts at:      0    1170    3582 
   Size:       1170    2412
 
DraIII CAC_nnn'GTG

Cuts at:      0    3150    3582 
   Size:       3150     432
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     130    3441    3582 
   Size:        130    3311     141

  Fragments arranged by size: 

                3311     141     130
 
EaeI y'GGCC_r

Cuts at:      0     113     173     429    1299    3011    3398    3582 
   Size:        113      60     256     870    1712     387     184

  Fragments arranged by size: 

                1712     870     387     256     184     113      60
 
EagI C'GGCC_G

Cuts at:      0     173    3582 
   Size:        173    3409
 
EarI CTCTTCn'nnn_

Cuts at:      0     154     339     535     796    3010    3368    3576    3582 
   Size:        154     185     196     261    2214     358     208       6

  Fragments arranged by size: 

                2214     358     261     208     196     185     154       6
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2954    3582 
   Size:       2954     628
 
EcoICRI GAG'CTC

Cuts at:      0    1887    3582 
   Size:       1887    1695
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     474     508    1016    1409    1481    1860    1960    3034 
   Size:        474      34     508     393      72     379     100    1074

Cuts at:   3034    3085    3105    3301    3582 
   Size:         51      20     196     281

  Fragments arranged by size: 

                1074     508     474     393     379     281     196     100
                  72      51      34      20
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    3028    3582 
   Size:       3028     554
 
EcoO109I rG'GnC_Cy

Cuts at:      0    3160    3387    3582 
   Size:       3160     227     195

  Fragments arranged by size: 

                3160     227     195
 
EcoRV GAT'ATC

Cuts at:      0    1440    3582 
   Size:       1440    2142
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2245    3582 
   Size:       2245    1337
 
FspI TGC'GCA

Cuts at:      0     823    3582 
   Size:        823    2759
 
HaeII r_GCGC'y

Cuts at:      0     108     905     915    1983    3582 
   Size:        108     797      10    1068    1599

  Fragments arranged by size: 

                1599    1068     797     108      10
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     119     151     418     450     845     877     959     991 
   Size:        119      32     267      32     395      32      82      32

Cuts at:    991    1027    1059    2273    2305    3582 
   Size:         36      32    1214      32    1277

  Fragments arranged by size: 

                1277    1214     395     267     119      82      36      32
                  32      32      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1194    1688    3582 
   Size:       1194     494    1894

  Fragments arranged by size: 

                1894    1194     494
 
KasI G'GCGC_C

Cuts at:      0     911    3582 
   Size:        911    2671
 
MfeI C'AATT_G

Cuts at:      0     666    3582 
   Size:        666    2916
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     236    1470    2630    3582 
   Size:        236    1234    1160     952

  Fragments arranged by size: 

                1234    1160     952     236
 
MscI TGG'CCA

Cuts at:      0     115    1301    3582 
   Size:        115    1186    2281

  Fragments arranged by size: 

                2281    1186     115
 
MslI CAynn'nnrTG

Cuts at:      0     508     883    1744    1861    2929    3582 
   Size:        508     375     861     117    1068     653

  Fragments arranged by size: 

                1068     861     653     508     375     117
 
MspA1I CmG'CkG

Cuts at:      0    3237    3243    3280    3582 
   Size:       3237       6      37     302

  Fragments arranged by size: 

                3237     302      37       6
 
NarI GG'CG_CC

Cuts at:      0     912    3582 
   Size:        912    2670
 
NcoI C'CATG_G

Cuts at:      0    1256    2924    3582 
   Size:       1256    1668     658

  Fragments arranged by size: 

                1668    1256     658
 
NdeI CA'TA_TG

Cuts at:      0    2723    3582 
   Size:       2723     859
 
NheI G'CTAG_C

Cuts at:      0    2394    3582 
   Size:       2394    1188
 
NsiI A_TGCA'T

Cuts at:      0    2576    3582 
   Size:       2576    1006
 
NspI r_CATG'y

Cuts at:      0    1281    2490    2574    2578    3582 
   Size:       1281    1209      84       4    1004

  Fragments arranged by size: 

                1281    1209    1004      84       4
 
PasI CC'CwG_GG

Cuts at:      0     300    3582 
   Size:        300    3282
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     234     255    3108    3582 
   Size:        234      21    2853     474

  Fragments arranged by size: 

                2853     474     234      21
 
PfoI T'CCnGG_A

Cuts at:      0     314    1140    1214    1425    3134    3317    3582 
   Size:        314     826      74     211    1709     183     265

  Fragments arranged by size: 

                1709     826     314     265     211     183      74
 
PmlI CAC'GTG

Cuts at:      0    1305    2748    3582 
   Size:       1305    1443     834

  Fragments arranged by size: 

                1443    1305     834
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     229     261    3008    3040    3582 
   Size:        229      32    2747      32     542

  Fragments arranged by size: 

                2747     542     229      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    3387    3582 
   Size:       3387     195
 
PshAI GACnn'nnGTC

Cuts at:      0     676    3582 
   Size:        676    2906
 
PstI C_TGCA'G

Cuts at:      0     687    1028    1274    1454    3227    3242    3395    3582 
   Size:        687     341     246     180    1773      15     153     187

  Fragments arranged by size: 

                1773     687     341     246     187     180     153      15
            
 
PvuII CAG'CTG

Cuts at:      0    3243    3582 
   Size:       3243     339
 
SacI G_AGCT'C

Cuts at:      0    1889    3582 
   Size:       1889    1693
 
SacII CC_GC'GG

Cuts at:      0    3281    3582 
   Size:       3281     301
 
SapI GCTCTTCn'nnn_

Cuts at:      0     535     796    3576    3582 
   Size:        535     261    2780       6

  Fragments arranged by size: 

                2780     535     261       6
 
SbfI CC_TGCA'GG

Cuts at:      0    3395    3582 
   Size:       3395     187
 
SexAI A'CCwGG_T

Cuts at:      0    2691    3582 
   Size:       2691     891
 
SfcI C'TryA_G

Cuts at:      0     497     683     783    1024    1270    1450    3223    3238 
   Size:        497     186     100     241     246     180    1773      15

Cuts at:   3238    3391    3582 
   Size:        153     191

  Fragments arranged by size: 

                1773     497     246     241     191     186     180     153
                 100      15
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1307    3582 
   Size:       1307    2275
 
SfoI GGC'GCC

Cuts at:      0     913    3582 
   Size:        913    2669
 
SmaI CCC'GGG

Cuts at:      0    3174    3582 
   Size:       3174     408
 
SmlI C'TyrA_G

Cuts at:      0    1756    3582 
   Size:       1756    1826
 
SphI G_CATG'C

Cuts at:      0    2574    3582 
   Size:       2574    1008
 
StuI AGG'CCT

Cuts at:      0     755    3582 
   Size:        755    2827
 
StyI C'CwwG_G

Cuts at:      0     845     915    1256    1917    2711    2811    2924    3029 
   Size:        845      70     341     661     794     100     113     105

Cuts at:   3029    3582 
   Size:        553

  Fragments arranged by size: 

                 845     794     661     553     341     113     105     100
                  70
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     469     827    2242    3582 
   Size:        469     358    1415    1340

  Fragments arranged by size: 

                1415    1340     469     358
 
TatI w'GTAC_w

Cuts at:      0     590    1454    1842    2372    2504    3582 
   Size:        590     864     388     530     132    1078

  Fragments arranged by size: 

                1078     864     590     530     388     132
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1077    1109    1382    1414    2690    2722    3582 
   Size:       1077      32     273      32    1276      32     860

  Fragments arranged by size: 

                1276    1077     860     273      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     277     652    3582 
   Size:        277     375    2930

  Fragments arranged by size: 

                2930     375     277
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     297     852    1254    3582 
   Size:        297     555     402    2328

  Fragments arranged by size: 

                2328     555     402     297
 
XmaI C'CCGG_G

Cuts at:      0    3172    3582 
   Size:       3172     410
 

 Enzymes that do cut:

     AarI     AccI     AclI     AcuI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI    ApaLI     AvaI     BaeI     BanI
    BanII     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI   BstEII    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRV     FalI     FspI    HaeII    Hin4I  HindIII     KasI     MfeI
     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NdeI     NheI
     NsiI     NspI     PasI    PflMI     PfoI     PmlI     PpiI    PpuMI
    PshAI     PstI    PvuII     SacI    SacII     SapI     SbfI    SexAI
     SfcI     SfiI     SfoI     SmaI     SmlI     SphI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I    AflII     ApaI     ApoI     AscI     AseI    AsiSI
    AvrII    BamHI    BbvCI    BmgBI     BmrI    BsiWI    BspEI   BssHII
    BstBI  BstZ17I     ClaI    CspCI    EcoRI     FseI    FspAI   HincII
     HpaI     KpnI     MluI     NaeI   NgoMIV     NotI     NruI     PacI
     PciI     PmeI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SalI    SanDI     ScaI     SgfI    SgrAI    SnaBI     SpeI     SrfI
     SspI     SwaI     XbaI     XhoI     XmnI     ZraI
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