Restriction map for pF1KE0369
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AviII       AvrII       
BamHI       BanI        BbeI        BbrPI       BbsI        
BcgI        BciVI       BclI        BfrI        BglI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        Hin1I       HincII      
HindII      HindIII     HinfI       HpaI        KasI        
KpnI        KspI        MamI        MfeI        MroI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RsrII       SacII       
SalI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        SspBI       
SspI        StuI        SwaI        TfiI        XbaI        
XhoI        


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# ONE CUT ENZYMES (   37 enzymes) #
###################################

AfaI          567  GTAC                 GT^AC
AflIII        853  ACGCGT               A^C[GA][CT]GT
ApaI           14  GGGCCC               GGGCC^C
Asp700I       534  GAAACTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         139  GAGCTC               G[AT]GC[AT]^C
AspI         1228  GACGAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII         295  GGACC                G^G[AT]CC
BsaI          821  GAGACC               GGTCTC(1/5)
BsiEI         195  CGGTCG               CG[GA][CT]^CG
BsiHKAI       139  GAGCTC               G[AT]GC[AT]^C
BspHI         800  TCATGA               T^CATGA
BssHII        311  GCGCGC               G^CGCGC
BstXI        1044  CCAGAGGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI         1360  TTTAAA               TTT^AAA
DraIII        393  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI         375  CCTCAGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI          363  CCCGC                CCCGC(4/6)
HaeII         505  AGCGCC               [GA]GCGC^[CT]
HgaI          441  GACGC                GACGC(5/10)
MluI          853  ACGCGT               A^CGCGT
MslI          916  CACCTCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I         91  CAGCGG               C[AC]G^C[GT]G
PflFI        1228  GACGAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         381  CCAGGCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         294  GGGACCC              [GA]G^G[AT]CC[CT]
RcaI          800  TCATGA               T^CATGA
RsaI          567  GTAC                 GT^AC
SacI          139  GAGCTC               GAGCT^C
SbfI           48  CCTGCAGG             CCTGCA^GG
ScaI          566  AGTACT               AGT^ACT
SmlI          826  CTCAAG               C^T[CT][GA]AG
Sse8387I       48  CCTGCAGG             CCTGCA^GG
TaqI          542  TCGA                 T^CGA
Tth111I      1228  GACGAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       542  TCGA                 T^CGA
Van91I        381  CCAGGCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          534  GAAACTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcsI         1237  AAATTC               [GA]^AATT[CT]
             1486  GAATTT              
ApoI         1237  AAATTC               [GA]^AATT[CT]
             1486  GAATTT              
AvaI          118  CCCGGG               C^[CT]CG[GA]G
              354  CCCGGG              
BanII          14  GGGCCC               G[GA]GC[CT]^C
              139  GAGCTC              
BbvCI         375  CCTCAGC              CCTCAGC(-5/-2)
              454  GCTGAGG             
BfaI          220  CTAG                 C^TAG
              259  CTAG                
BseRI         289  GAGGAG               GAGGAG(10/8)
             1618  GAGGAG              
BsgI          913  CTGCAC               GTGCAG(16/14)
              268  GTGCAG              
BsmAI         821  GAGAC                GTCTC(1/5)
              754  GTCTC               
BsoBI         118  CCCGGG               C^[CT]CG[GA]G
              354  CCCGGG              
BspMI          47  ACCTGC               ACCTGC(4/8)
              989  GCAGGT              
BstYI        1079  AGATCC               [GA]^GATC[CT]
             1203  GGATCT              
MaeI          220  CTAG                 C^TAG
              259  CTAG                
MflI         1079  AGATCC               [GA]^GATC[CT]
             1203  GGATCT              
MseI          488  TTAA                 T^TAA
             1361  TTAA                
NcoI          461  CCATGG               C^CATGG
             1127  CCATGG              
SapI          421  GAAGAGC              GCTCTTC(1/4)
             1284  GCTCTTC             
SmaI          118  CCCGGG               CCC^GGG
              354  CCCGGG              
Tru9I         488  TTAA                 T^TAA
             1361  TTAA                
XcmI          894  CCAGATAGCCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1058  CCAATGGTTACATGG     
XhoII        1079  AGATCC               [GA]^GATC[CT]
             1203  GGATCT              
XmaCI         118  CCCGGG               C^CCGGG
              354  CCCGGG              
XmaI          118  CCCGGG               C^CCGGG
              354  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        AlwNI       
BalI        BbvI        BcnI        BmyI        BsaJI       
BsiYI       BslI        BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MluNI       MnlI        MscI        MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PstI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TseI        Tsp45I      
Tsp509I     TspRI       
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