Restriction map for
pF1KE0369
(Circular) (Six-base) MAPSORT of: pF1KE0369.seq Check: 1811 from: 1 to: 4772
pF1KE0369 4772 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 1810 1810
Size: 4772
Acc65I G'GTAC_C
Cuts at: 1788 1788
Size: 4772
AclI AA'CG_TT
Cuts at: 4612 4612
Size: 4772
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 808 1336 1517 2597 3029 3776 4044 808
Size: 528 181 1080 432 747 268 1536
Fragments arranged by size:
1536 1080 747 528 432 268 181
AflIII A'CryG_T
Cuts at: 961 2124 3228 4205 961
Size: 1163 1104 977 1528
Fragments arranged by size:
1528 1163 1104 977
AgeI A'CCGG_T
Cuts at: 3160 3160
Size: 4772
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 211 211
Size: 4772
AlwNI CAG_nnn'CTG
Cuts at: 261 432 1642 2077 3644 261
Size: 171 1210 435 1567 1389
Fragments arranged by size:
1567 1389 1210 435 171
ApaI G_GGCC'C
Cuts at: 126 126
Size: 4772
ApaLI G'TGCA_C
Cuts at: 3542 3542
Size: 4772
ApoI r'AATT_y
Cuts at: 1345 1594 1776 4125 4381 1345
Size: 249 182 2349 256 1736
Fragments arranged by size:
2349 1736 256 249 182
AseI AT'TA_AT
Cuts at: 20 20
Size: 4772
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4772
AvaI C'yCGr_G
Cuts at: 226 462 1792 226
Size: 236 1330 3206
Fragments arranged by size:
3206 1330 236
BamHI G'GATC_C
Cuts at: 1797 1797
Size: 4772
BanI G'GyrC_C
Cuts at: 1788 2202 2450 2485 1788
Size: 414 248 35 4075
Fragments arranged by size:
4075 414 248 35
BanII G_rGCy'C
Cuts at: 126 251 1786 2816 126
Size: 125 1535 1030 2082
Fragments arranged by size:
2082 1535 1030 125
BbeI G_GCGC'C
Cuts at: 2206 2454 2206
Size: 248 4524
BbvCI CC'TCA_GC
Cuts at: 484 563 484
Size: 79 4693
BciVI GTATCCnnnnn_n'
Cuts at: 2670 3431 4772 2670
Size: 761 1341 2670
Fragments arranged by size:
2670 1341 761
BclI T'GATC_A
Cuts at: 2292 2292
Size: 4772
BglII A'GATC_T
Cuts at: 2287 4324 2287
Size: 2037 2735
BlpI GC'TnA_GC
Cuts at: 1884 1884
Size: 4772
Bme1580I G_kGCm'C
Cuts at: 126 341 456 961 2397 2490 3546 126
Size: 215 115 505 1436 93 1056 1352
Fragments arranged by size:
1436 1352 1056 505 215 115 93
BmgBI CAC'GTC
Cuts at: 375 375
Size: 4772
BmrI ACTGGGnnnn_n'
Cuts at: 393 755 1984 2065 2400 4544 393
Size: 362 1229 81 335 2144 621
Fragments arranged by size:
2144 1229 621 362 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3139 3139
Size: 4772
Bpu10I CC'TnA_GC
Cuts at: 484 563 1422 484
Size: 79 859 3834
Fragments arranged by size:
3834 859 79
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 919 1948 2827 3319 3617 919
Size: 1029 879 492 298 2074
Fragments arranged by size:
2074 1029 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 923 4476 923
Size: 3553 1219
BsaAI yAC'GTr
Cuts at: 2125 2125
Size: 4772
BsaBI GATnn'nnATC
Cuts at: 6 2286 4323 6
Size: 2280 2037 455
Fragments arranged by size:
2280 2037 455
BsaHI Gr'CG_yC
Cuts at: 2203 2451 3153 4649 2203
Size: 248 702 1496 2326
Fragments arranged by size:
2326 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1961 2482 3160 3434 3581 3992 1961
Size: 521 678 274 147 411 2741
Fragments arranged by size:
2741 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 412 1741 412
Size: 1329 3443
BseYI C'CCAG_C
Cuts at: 696 2197 2955 3532 4028 696
Size: 1501 758 577 496 1440
Fragments arranged by size:
1501 1440 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 1006 397
Size: 609 4163
BsiEI CG_ry'CG
Cuts at: 104 306 2314 2360 3568 104
Size: 202 2008 46 1208 1308
Fragments arranged by size:
2008 1308 1208 202 46
BsiHKAI G_wGCw'C
Cuts at: 251 1786 2564 2754 3546 251
Size: 1535 778 190 792 1477
Fragments arranged by size:
1535 1477 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 126 251 341 456 961 1786 2397 2490 2564
Size: 125 90 115 505 825 611 93 74
Cuts at: 2564 2754 2816 3546 126
Size: 190 62 730 1352
Fragments arranged by size:
1352 825 730 611 505 190 125 115
93 90 74 62
BspEI T'CCGG_A
Cuts at: 3992 3992
Size: 4772
BspHI T'CATG_A
Cuts at: 908 3948 908
Size: 3040 1732
BspMI ACCTGCnnnn'nnnn_
Cuts at: 164 1088 1822 2036 2338 2719 164
Size: 924 734 214 302 381 2217
Fragments arranged by size:
2217 924 734 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3064 3118 4602 4767 50
Size: 3014 54 1484 165 55
Fragments arranged by size:
3014 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2684 2684
Size: 4772
BsrFI r'CCGG_y
Cuts at: 2770 2951 3160 2770
Size: 181 209 4382
Fragments arranged by size:
4382 209 181
BssHII G'CGCG_C
Cuts at: 419 419
Size: 4772
BssSI C'ACGA_G
Cuts at: 3043 3401 3043
Size: 358 4414
BstAPI GCAn_nnn'nTGC
Cuts at: 2149 2149
Size: 4772
BstBI TT'CG_AA
Cuts at: 3133 3133
Size: 4772
BstXI CCAn_nnnn'nTGG
Cuts at: 1159 1159
Size: 4772
BstYI r'GATC_y
Cuts at: 1 1187 1311 1797 2264 2287 2622 2868 3880
Size: 1186 124 486 467 23 335 246 1012
Cuts at: 3880 3966 3978 4324 1
Size: 86 12 346 449
Fragments arranged by size:
1186 1012 486 467 449 346 335 246
124 86 23 12
BtgI C'CryG_G
Cuts at: 107 494 569 1121 1235 107
Size: 387 75 552 114 3644
Fragments arranged by size:
3644 552 387 114 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1539 2722 2903 3066 1539
Size: 1183 181 163 3245
Fragments arranged by size:
3245 1183 181 163
BtsI GCAGTG_nn'
Cuts at: 551 643 2015 551
Size: 92 1372 3308
Fragments arranged by size:
3308 1372 92
DraI TTT'AAA
Cuts at: 1470 1771 1470
Size: 301 4471
DraIII CAC_nnn'GTG
Cuts at: 506 506
Size: 4772
DrdI GACnn_nn'nnGTC
Cuts at: 2478 3336 2478
Size: 858 3914
EaeI y'GGCC_r
Cuts at: 392 437 497 554 1118 2357 2531 2922 2949
Size: 45 60 57 564 1239 174 391 27
Cuts at: 2949 4294 392
Size: 1345 870
Fragments arranged by size:
1345 1239 870 564 391 174 60 57
45 27
EagI C'GGCC_G
Cuts at: 2357 2357
Size: 4772
EarI CTCTTCn'nnn_
Cuts at: 319 524 652 1399 2795 3005 319
Size: 205 128 747 1396 210 2086
Fragments arranged by size:
2086 1396 747 210 205 128
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 241 2980 3290 3436 4582 241
Size: 2739 310 146 1146 431
Fragments arranged by size:
2739 1146 431 310 146
EcoICRI GAG'CTC
Cuts at: 249 1784 249
Size: 1535 3237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 808 1336 1517 2597 3029 3776 4044 808
Size: 528 181 1080 432 747 268 1536
Fragments arranged by size:
1536 1080 747 528 432 268 181
EcoNI CCTnn'n_nnAGG
Cuts at: 487 487
Size: 4772
EcoO109I rG'GnC_Cy
Cuts at: 122 123 403 1911 122
Size: 1 280 1508 2983
Fragments arranged by size:
2983 1508 280 1
EcoRI G'AATT_C
Cuts at: 1776 1776
Size: 4772
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 849 1635 2791 849
Size: 786 1156 2830
Fragments arranged by size:
2830 1156 786
FspI TGC'GCA
Cuts at: 2553 2553
Size: 4772
HaeII r_GCGC'y
Cuts at: 617 2206 2454 3476 4455 617
Size: 1589 248 1022 979 934
Fragments arranged by size:
1589 1022 979 934 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1558 1590 2614 2644 2646 2676 1558
Size: 32 1024 30 2 30 3654
Fragments arranged by size:
3654 1024 32 30 30 2
HincII GTy'rAC
Cuts at: 1811 1811
Size: 4772
KasI G'GCGC_C
Cuts at: 2202 2450 2202
Size: 248 4524
KpnI G_GTAC'C
Cuts at: 1792 1792
Size: 4772
MluI A'CGCG_T
Cuts at: 961 4205 961
Size: 3244 1528
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3443 3627 3443
Size: 184 4588
MscI TGG'CCA
Cuts at: 439 499 556 2533 439
Size: 60 57 1977 2678
Fragments arranged by size:
2678 1977 60 57
MslI CAynn'nnrTG
Cuts at: 1028 2761 2888 1028
Size: 1733 127 2912
Fragments arranged by size:
2912 1733 127
MspA1I CmG'CkG
Cuts at: 201 1883 2197 2557 3570 3815 201
Size: 1682 314 360 1013 245 1158
Fragments arranged by size:
1682 1158 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2953 2953
Size: 4772
NarI GG'CG_CC
Cuts at: 2203 2451 2203
Size: 248 4524
NcoI C'CATG_G
Cuts at: 107 569 1235 107
Size: 462 666 3644
Fragments arranged by size:
3644 666 462
NgoMIV G'CCGG_C
Cuts at: 2951 2951
Size: 4772
NspI r_CATG'y
Cuts at: 1825 2325 3232 1825
Size: 500 907 3365
Fragments arranged by size:
3365 907 500
PciI A'CATG_T
Cuts at: 3228 3228
Size: 4772
PflMI CCAn_nnn'nTGG
Cuts at: 495 495
Size: 4772
PmeI GTTT'AAAC
Cuts at: 1771 1771
Size: 4772
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3911 3943 3911
Size: 32 4740
PpuMI rG'GwC_Cy
Cuts at: 403 403
Size: 4772
PspOMI G'GGCC_C
Cuts at: 122 122
Size: 4772
PstI C_TGCA'G
Cuts at: 161 200 236 428 546 638 1099 1819 2504
Size: 39 36 192 118 92 461 720 685
Cuts at: 2504 161
Size: 2429
Fragments arranged by size:
2429 720 685 461 192 118 92 39
36
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4772
PvuII CAG'CTG
Cuts at: 2197 2557 2197
Size: 360 4412
RsrII CG'GwC_CG
Cuts at: 2967 2967
Size: 4772
SacI G_AGCT'C
Cuts at: 251 1786 251
Size: 1535 3237
SalI G'TCGA_C
Cuts at: 1809 1809
Size: 4772
SanDI GG'GwC_CC
Cuts at: 403 403
Size: 4772
SapI GCTCTTCn'nnn_
Cuts at: 524 1399 2795 3005 524
Size: 875 1396 210 2291
Fragments arranged by size:
2291 1396 875 210
SbfI CC_TGCA'GG
Cuts at: 161 1819 161
Size: 1658 3114
ScaI AGT'ACT
Cuts at: 676 4289 676
Size: 3613 1159
SfcI C'TryA_G
Cuts at: 33 157 196 232 424 542 634 1095 1649
Size: 124 39 36 192 118 92 461 554
Cuts at: 1649 1815 2500 3493 3684 33
Size: 166 685 993 191 1121
Fragments arranged by size:
1121 993 685 554 461 192 191 166
124 118 92 39 36
SfoI GGC'GCC
Cuts at: 2204 2452 2204
Size: 248 4524
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4772
SmaI CCC'GGG
Cuts at: 228 464 1794 228
Size: 236 1330 3206
Fragments arranged by size:
3206 1330 236
SmlI C'TyrA_G
Cuts at: 934 1927 2842 3334 3596 934
Size: 993 915 492 262 2110
Fragments arranged by size:
2110 993 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4772
SphI G_CATG'C
Cuts at: 1825 2325 1825
Size: 500 4272
SrfI GCCC'GGGC
Cuts at: 228 228
Size: 4772
StyI C'CwwG_G
Cuts at: 107 557 569 1235 1906 2260 107
Size: 450 12 666 671 354 2619
Fragments arranged by size:
2619 671 666 450 354 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 292 1165 2355 2488 2947 3156 292
Size: 873 1190 133 459 209 1908
Fragments arranged by size:
1908 1190 873 459 209 133
TatI w'GTAC_w
Cuts at: 674 4287 674
Size: 3613 1159
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4289 4321 4289
Size: 32 4740
Tth111I GACn'n_nGTC
Cuts at: 1339 2569 1339
Size: 1230 3542
XbaI T'CTAG_A
Cuts at: 1803 1803
Size: 4772
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1009 1173 2267 114
Size: 895 164 1094 2619
Fragments arranged by size:
2619 1094 895 164
XmaI C'CCGG_G
Cuts at: 226 462 1792 226
Size: 236 1330 3206
Fragments arranged by size:
3206 1330 236
XmnI GAAnn'nnTTC
Cuts at: 646 646
Size: 4772
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SrfI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbsI BcgI BfrBI BglI BmtI BpmI
BsaXI BsiWI BsmI BsmBI BsrGI BstEII BstZ17I Bsu36I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PshAI PsiI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SspI StuI SwaI XhoI ZraI