Restriction map for pF1KE0369
 (Linear) (Six-base) MAPSORT of: KIEE0369.seq  Check: 9753  from: 1  to: 1659



KIEE0369.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     700    1228    1409    1659 
   Size:        700     528     181     250

  Fragments arranged by size: 

                 700     528     250     181
 
AflIII A'CryG_T

Cuts at:      0     853    1659 
   Size:        853     806
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     103    1659 
   Size:        103    1556
 
AlwNI CAG_nnn'CTG

Cuts at:      0     153     324    1534    1659 
   Size:        153     171    1210     125

  Fragments arranged by size: 

                1210     171     153     125
 
ApaI G_GGCC'C

Cuts at:      0      18    1659 
   Size:         18    1641
 
ApoI r'AATT_y

Cuts at:      0    1237    1486    1659 
   Size:       1237     249     173

  Fragments arranged by size: 

                1237     249     173
 
AvaI C'yCGr_G

Cuts at:      0     118     354    1659 
   Size:        118     236    1305

  Fragments arranged by size: 

                1305     236     118
 
BanII G_rGCy'C

Cuts at:      0      18     143    1659 
   Size:         18     125    1516

  Fragments arranged by size: 

                1516     125      18
 
BbvCI CC'TCA_GC

Cuts at:      0     376     455    1659 
   Size:        376      79    1204

  Fragments arranged by size: 

                1204     376      79
 
Bme1580I G_kGCm'C

Cuts at:      0      18     233     348     853    1659 
   Size:         18     215     115     505     806

  Fragments arranged by size: 

                 806     505     215     115      18
 
BmgBI CAC'GTC

Cuts at:      0     267    1659 
   Size:        267    1392
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     285     647    1659 
   Size:        285     362    1012

  Fragments arranged by size: 

                1012     362     285
 
Bpu10I CC'TnA_GC

Cuts at:      0     376     455    1314    1659 
   Size:        376      79     859     345

  Fragments arranged by size: 

                 859     376     345      79
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     811    1659 
   Size:        811     848
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     815    1659 
   Size:        815     844
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     304    1633    1659 
   Size:        304    1329      26

  Fragments arranged by size: 

                1329     304      26
 
BseYI C'CCAG_C

Cuts at:      0     588    1659 
   Size:        588    1071
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     289     898    1659 
   Size:        289     609     761

  Fragments arranged by size: 

                 761     609     289
 
BsiEI CG_ry'CG

Cuts at:      0     198    1659 
   Size:        198    1461
 
BsiHKAI G_wGCw'C

Cuts at:      0     143    1659 
   Size:        143    1516
 
Bsp1286I G_dGCh'C

Cuts at:      0      18     143     233     348     853    1659 
   Size:         18     125      90     115     505     806

  Fragments arranged by size: 

                 806     505     125     115      90      18
 
BspHI T'CATG_A

Cuts at:      0     800    1659 
   Size:        800     859
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      56     980    1659 
   Size:         56     924     679

  Fragments arranged by size: 

                 924     679      56
 
BssHII G'CGCG_C

Cuts at:      0     311    1659 
   Size:        311    1348
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1051    1659 
   Size:       1051     608
 
BstYI r'GATC_y

Cuts at:      0    1079    1203    1659 
   Size:       1079     124     456

  Fragments arranged by size: 

                1079     456     124
 
BtgI C'CryG_G

Cuts at:      0     386     461    1013    1127    1659 
   Size:        386      75     552     114     532

  Fragments arranged by size: 

                 552     532     386     114      75
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1431    1659 
   Size:       1431     228
 
BtsI GCAGTG_nn'

Cuts at:      0     443     535    1659 
   Size:        443      92    1124

  Fragments arranged by size: 

                1124     443      92
 
DraI TTT'AAA

Cuts at:      0    1362    1659 
   Size:       1362     297
 
DraIII CAC_nnn'GTG

Cuts at:      0     398    1659 
   Size:        398    1261
 
EaeI y'GGCC_r

Cuts at:      0     284     329     389     446    1010    1659 
   Size:        284      45      60      57     564     649

  Fragments arranged by size: 

                 649     564     284      60      57      45
 
EarI CTCTTCn'nnn_

Cuts at:      0     211     416     544    1291    1659 
   Size:        211     205     128     747     368

  Fragments arranged by size: 

                 747     368     211     205     128
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     133    1659 
   Size:        133    1526
 
EcoICRI GAG'CTC

Cuts at:      0     141    1659 
   Size:        141    1518
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     700    1228    1409    1659 
   Size:        700     528     181     250

  Fragments arranged by size: 

                 700     528     250     181
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     379    1659 
   Size:        379    1280
 
EcoO109I rG'GnC_Cy

Cuts at:      0      14      15     295    1659 
   Size:         14       1     280    1364

  Fragments arranged by size: 

                1364     280      14       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     741    1527    1659 
   Size:        741     786     132

  Fragments arranged by size: 

                 786     741     132
 
HaeII r_GCGC'y

Cuts at:      0     509    1659 
   Size:        509    1150
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1450    1482    1659 
   Size:       1450      32     177

  Fragments arranged by size: 

                1450     177      32
 
MluI A'CGCG_T

Cuts at:      0     853    1659 
   Size:        853     806
 
MscI TGG'CCA

Cuts at:      0     331     391     448    1659 
   Size:        331      60      57    1211

  Fragments arranged by size: 

                1211     331      60      57
 
MslI CAynn'nnrTG

Cuts at:      0     920    1659 
   Size:        920     739
 
MspA1I CmG'CkG

Cuts at:      0      93    1659 
   Size:         93    1566
 
NcoI C'CATG_G

Cuts at:      0     461    1127    1659 
   Size:        461     666     532

  Fragments arranged by size: 

                 666     532     461
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     387    1659 
   Size:        387    1272
 
PpuMI rG'GwC_Cy

Cuts at:      0     295    1659 
   Size:        295    1364
 
PspOMI G'GGCC_C

Cuts at:      0      14    1659 
   Size:         14    1645
 
PstI C_TGCA'G

Cuts at:      0      53      92     128     320     438     530     991    1659 
   Size:         53      39      36     192     118      92     461     668

  Fragments arranged by size: 

                 668     461     192     118      92      53      39      36
            
 
SacI G_AGCT'C

Cuts at:      0     143    1659 
   Size:        143    1516
 
SanDI GG'GwC_CC

Cuts at:      0     295    1659 
   Size:        295    1364
 
SapI GCTCTTCn'nnn_

Cuts at:      0     416    1291    1659 
   Size:        416     875     368

  Fragments arranged by size: 

                 875     416     368
 
SbfI CC_TGCA'GG

Cuts at:      0      53    1659 
   Size:         53    1606
 
ScaI AGT'ACT

Cuts at:      0     568    1659 
   Size:        568    1091
 
SfcI C'TryA_G

Cuts at:      0      49      88     124     316     434     526     987    1541 
   Size:         49      39      36     192     118      92     461     554

Cuts at:   1541    1659 
   Size:        118

  Fragments arranged by size: 

                 554     461     192     118     118      92      49      39
                  36
 
SmaI CCC'GGG

Cuts at:      0     120     356    1659 
   Size:        120     236    1303

  Fragments arranged by size: 

                1303     236     120
 
SmlI C'TyrA_G

Cuts at:      0     826    1659 
   Size:        826     833
 
SrfI GCCC'GGGC

Cuts at:      0     120    1659 
   Size:        120    1539
 
StyI C'CwwG_G

Cuts at:      0     449     461    1127    1659 
   Size:        449      12     666     532

  Fragments arranged by size: 

                 666     532     449      12
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     184    1057    1659 
   Size:        184     873     602

  Fragments arranged by size: 

                 873     602     184
 
TatI w'GTAC_w

Cuts at:      0     566    1659 
   Size:        566    1093
 
Tth111I GACn'n_nGTC

Cuts at:      0    1231    1659 
   Size:       1231     428
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     901    1065    1659 
   Size:        901     164     594

  Fragments arranged by size: 

                 901     594     164
 
XmaI C'CCGG_G

Cuts at:      0     118     354    1659 
   Size:        118     236    1305

  Fragments arranged by size: 

                1305     236     118
 
XmnI GAAnn'nnTTC

Cuts at:      0     538    1659 
   Size:        538    1121
 

 Enzymes that do cut:

     AcuI   AflIII     AlfI    AlwNI     ApaI     ApoI     AvaI    BanII
    BbvCI Bme1580I    BmgBI     BmrI   Bpu10I    BpuEI     BsaI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspHI    BspMI   BssHII
    BstXI    BstYI     BtgI    BtgZI     BtsI     DraI   DraIII     EaeI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    HaeII
    Hin4I     MluI     MscI     MslI   MspA1I     NcoI    PflMI    PpuMI
   PspOMI     PstI     SacI    SanDI     SapI     SbfI     ScaI     SfcI
     SmaI     SmlI     SrfI     StyI    TaqII     TatI  Tth111I     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BanI     BbeI     BbsI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI     BmtI     BplI     BpmI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BsiWI     BsmI    BsmBI    BspEI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I     ClaI    CspCI     DrdI     EagI    EcoRI    EcoRV
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII
    RsrII    SacII     SalI    SexAI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SspI     StuI     SwaI     TstI     XbaI
     XhoI     ZraI
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