(Circular) (Six-base) MAPSORT of: pF1KB4372.seq Check: 9213 from: 1 to: 5294 pF1KB4372 5294 bp With 182 enzymes: SgfI * November 16, 2012 10:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1396 1396 Size: 5294 AccI GT'mk_AC Cuts at: 758 2332 758 Size: 1574 3720 Acc65I G'GTAC_C Cuts at: 2310 2310 Size: 5294 AclI AA'CG_TT Cuts at: 5134 5134 Size: 5294 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1193 1958 3119 3551 4298 4566 1193 Size: 765 1161 432 747 268 1921 Fragments arranged by size: 1921 1161 765 747 432 268 AflIII A'CryG_T Cuts at: 2646 3750 4727 2646 Size: 1104 977 3213 Fragments arranged by size: 3213 1104 977 AgeI A'CCGG_T Cuts at: 3682 3682 Size: 5294 AleI CACnn'nnGTG Cuts at: 1750 1750 Size: 5294 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 255 1047 255 Size: 792 4502 AlwNI CAG_nnn'CTG Cuts at: 291 2599 4166 291 Size: 2308 1567 1419 Fragments arranged by size: 2308 1567 1419 ApaLI G'TGCA_C Cuts at: 4064 4064 Size: 5294 ApoI r'AATT_y Cuts at: 313 940 1693 2105 2155 2298 4647 4903 313 Size: 627 753 412 50 143 2349 256 704 Fragments arranged by size: 2349 753 704 627 412 256 143 50 AseI AT'TA_AT Cuts at: 20 20 Size: 5294 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5294 AvaI C'yCGr_G Cuts at: 2314 2314 Size: 5294 BamHI G'GATC_C Cuts at: 2319 2319 Size: 5294 BanI G'GyrC_C Cuts at: 2310 2724 2972 3007 2310 Size: 414 248 35 4597 Fragments arranged by size: 4597 414 248 35 BanII G_rGCy'C Cuts at: 708 1537 2308 3338 708 Size: 829 771 1030 2664 Fragments arranged by size: 2664 1030 829 771 BbeI G_GCGC'C Cuts at: 2728 2976 2728 Size: 248 5046 BbsI GAAGACnn'nnnn_ Cuts at: 877 877 Size: 5294 BbvCI CC'TCA_GC Cuts at: 691 691 Size: 5294 BciVI GTATCCnnnnn_n' Cuts at: 3192 3953 5294 3192 Size: 761 1341 3192 Fragments arranged by size: 3192 1341 761 BclI T'GATC_A Cuts at: 2814 2814 Size: 5294 BglI GCCn_nnn'nGGC Cuts at: 1639 1639 Size: 5294 BglII A'GATC_T Cuts at: 2809 4846 2809 Size: 2037 3257 BlpI GC'TnA_GC Cuts at: 284 2406 284 Size: 2122 3172 Bme1580I G_kGCm'C Cuts at: 2919 3012 4068 2919 Size: 93 1056 4145 Fragments arranged by size: 4145 1056 93 BmgBI CAC'GTC Cuts at: 948 1768 948 Size: 820 4474 BmrI ACTGGGnnnn_n' Cuts at: 636 2506 2587 2922 5066 636 Size: 1870 81 335 2144 864 Fragments arranged by size: 2144 1870 864 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3661 3661 Size: 5294 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 712 712 Size: 5294 Bpu10I CC'TnA_GC Cuts at: 691 691 Size: 5294 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 850 2470 3349 3841 4139 850 Size: 1620 879 492 298 2005 Fragments arranged by size: 2005 1620 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4998 4998 Size: 5294 BsaAI yAC'GTr Cuts at: 2647 2647 Size: 5294 BsaBI GATnn'nnATC Cuts at: 6 1156 2808 4845 6 Size: 1150 1652 2037 455 Fragments arranged by size: 2037 1652 1150 455 BsaHI Gr'CG_yC Cuts at: 2725 2973 3675 5171 2725 Size: 248 702 1496 2848 Fragments arranged by size: 2848 1496 702 248 BsaWI w'CCGG_w Cuts at: 2483 3004 3682 3956 4103 4514 2483 Size: 521 678 274 147 411 3263 Fragments arranged by size: 3263 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 740 770 1161 1191 2273 2303 740 Size: 30 391 30 1082 30 3731 Fragments arranged by size: 3731 1082 391 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 164 350 353 164 Size: 186 3 5105 Fragments arranged by size: 5105 186 3 BseYI C'CCAG_C Cuts at: 1104 2719 3477 4054 4550 1104 Size: 1615 758 577 496 1848 Fragments arranged by size: 1848 1615 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1579 1728 1579 Size: 149 5145 BsiEI CG_ry'CG Cuts at: 104 1160 2836 2882 4090 104 Size: 1056 1676 46 1208 1308 Fragments arranged by size: 1676 1308 1208 1056 46 BsiHKAI G_wGCw'C Cuts at: 159 2308 3086 3276 4068 159 Size: 2149 778 190 792 1385 Fragments arranged by size: 2149 1385 792 778 190 Bsp1286I G_dGCh'C Cuts at: 159 708 1537 2308 2919 3012 3086 3276 3338 Size: 549 829 771 611 93 74 190 62 Cuts at: 3338 4068 159 Size: 730 1385 Fragments arranged by size: 1385 829 771 730 611 549 190 93 74 62 BspEI T'CCGG_A Cuts at: 4514 4514 Size: 5294 BspHI T'CATG_A Cuts at: 485 531 4470 485 Size: 46 3939 1309 Fragments arranged by size: 3939 1309 46 BspMI ACCTGCnnnn'nnnn_ Cuts at: 554 1396 2344 2558 2860 3241 554 Size: 842 948 214 302 381 2607 Fragments arranged by size: 2607 948 842 381 302 214 BsrBI CCG'CTC Cuts at: 50 905 3586 3640 5124 5289 50 Size: 855 2681 54 1484 165 55 Fragments arranged by size: 2681 1484 855 165 55 54 BsrDI GCAATG_nn' Cuts at: 577 800 3206 577 Size: 223 2406 2665 Fragments arranged by size: 2665 2406 223 BsrFI r'CCGG_y Cuts at: 2204 3292 3473 3682 2204 Size: 1088 181 209 3816 Fragments arranged by size: 3816 1088 209 181 BssSI C'ACGA_G Cuts at: 3565 3923 3565 Size: 358 4936 BstAPI GCAn_nnn'nTGC Cuts at: 2671 2671 Size: 5294 BstBI TT'CG_AA Cuts at: 3655 3655 Size: 5294 BstXI CCAn_nnnn'nTGG Cuts at: 846 846 Size: 5294 BstYI r'GATC_y Cuts at: 1 2319 2786 2809 3144 3390 4402 4488 4500 Size: 2318 467 23 335 246 1012 86 12 Cuts at: 4500 4846 1 Size: 346 449 Fragments arranged by size: 2318 1012 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 453 974 107 Size: 346 521 4427 Fragments arranged by size: 4427 521 346 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3244 3425 3588 3244 Size: 181 163 4950 Fragments arranged by size: 4950 181 163 BtsI GCAGTG_nn' Cuts at: 131 1745 2537 131 Size: 1614 792 2888 Fragments arranged by size: 2888 1614 792 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 2246 2281 2246 Size: 35 5259 DraI TTT'AAA Cuts at: 1478 2293 1478 Size: 815 4479 DrdI GACnn_nn'nnGTC Cuts at: 3000 3858 3000 Size: 858 4436 EaeI y'GGCC_r Cuts at: 1631 2879 3053 3444 3471 4816 1631 Size: 1248 174 391 27 1345 2109 Fragments arranged by size: 2109 1345 1248 391 174 27 EagI C'GGCC_G Cuts at: 2879 2879 Size: 5294 EarI CTCTTCn'nnn_ Cuts at: 1272 1990 2228 2244 3317 3527 1272 Size: 718 238 16 1073 210 3039 Fragments arranged by size: 3039 1073 718 238 210 16 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3502 3812 3958 5104 3502 Size: 310 146 1146 3692 Fragments arranged by size: 3692 1146 310 146 EcoICRI GAG'CTC Cuts at: 2306 2306 Size: 5294 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 712 1193 1958 3119 3551 4298 4566 712 Size: 481 765 1161 432 747 268 1440 Fragments arranged by size: 1440 1161 765 747 481 432 268 EcoO109I rG'GnC_Cy Cuts at: 979 2433 979 Size: 1454 3840 EcoRI G'AATT_C Cuts at: 2298 2298 Size: 5294 EcoRV GAT'ATC Cuts at: 321 469 1221 1377 321 Size: 148 752 156 4238 Fragments arranged by size: 4238 752 156 148 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 544 3313 544 Size: 2769 2525 FspI TGC'GCA Cuts at: 1000 3075 1000 Size: 2075 3219 HaeII r_GCGC'y Cuts at: 2728 2976 3998 4977 2728 Size: 248 1022 979 3045 Fragments arranged by size: 3045 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 400 403 432 435 740 772 1271 1303 1318 Size: 3 29 3 305 32 499 32 15 Cuts at: 1318 1333 1350 1365 1410 1442 1752 1784 3136 Size: 15 17 15 45 32 310 32 1352 Cuts at: 3136 3166 3168 3198 400 Size: 30 2 30 2496 Fragments arranged by size: 2496 1352 499 310 305 45 32 32 32 32 30 30 29 17 15 15 15 3 3 2 HincII GTy'rAC Cuts at: 258 1943 2333 258 Size: 1685 390 3219 Fragments arranged by size: 3219 1685 390 HpaI GTT'AAC Cuts at: 258 1943 258 Size: 1685 3609 KasI G'GCGC_C Cuts at: 2724 2972 2724 Size: 248 5046 KpnI G_GTAC'C Cuts at: 2314 2314 Size: 5294 MfeI C'AATT_G Cuts at: 666 752 666 Size: 86 5208 MluI A'CGCG_T Cuts at: 4727 4727 Size: 5294 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 126 473 1204 1653 3965 4149 126 Size: 347 731 449 2312 184 1271 Fragments arranged by size: 2312 1271 731 449 347 184 MscI TGG'CCA Cuts at: 1633 3055 1633 Size: 1422 3872 MslI CAynn'nnrTG Cuts at: 458 536 779 1750 3283 3410 458 Size: 78 243 971 1533 127 2342 Fragments arranged by size: 2342 1533 971 243 127 78 MspA1I CmG'CkG Cuts at: 2166 2405 2719 3079 4092 4337 2166 Size: 239 314 360 1013 245 3123 Fragments arranged by size: 3123 1013 360 314 245 239 NaeI GCC'GGC Cuts at: 2206 3475 2206 Size: 1269 4025 NarI GG'CG_CC Cuts at: 2725 2973 2725 Size: 248 5046 NcoI C'CATG_G Cuts at: 107 453 107 Size: 346 4948 NgoMIV G'CCGG_C Cuts at: 2204 3473 2204 Size: 1269 4025 NspI r_CATG'y Cuts at: 901 2347 2847 3754 901 Size: 1446 500 907 2441 Fragments arranged by size: 2441 1446 907 500 PciI A'CATG_T Cuts at: 3750 3750 Size: 5294 PflMI CCAn_nnn'nTGG Cuts at: 1045 1045 Size: 5294 PmeI GTTT'AAAC Cuts at: 2293 2293 Size: 5294 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4433 4465 4433 Size: 32 5262 PpuMI rG'GwC_Cy Cuts at: 979 979 Size: 5294 PsiI TTA'TAA Cuts at: 1865 1865 Size: 5294 PstI C_TGCA'G Cuts at: 476 1341 2232 2341 3026 476 Size: 865 891 109 685 2744 Fragments arranged by size: 2744 891 865 685 109 PvuI CG_AT'CG Cuts at: 104 1160 104 Size: 1056 4238 PvuII CAG'CTG Cuts at: 2166 2719 3079 2166 Size: 553 360 4381 Fragments arranged by size: 4381 553 360 RsrII CG'GwC_CG Cuts at: 3489 3489 Size: 5294 SacI G_AGCT'C Cuts at: 2308 2308 Size: 5294 SalI G'TCGA_C Cuts at: 2331 2331 Size: 5294 SapI GCTCTTCn'nnn_ Cuts at: 3317 3527 3317 Size: 210 5084 SbfI CC_TGCA'GG Cuts at: 2341 2341 Size: 5294 ScaI AGT'ACT Cuts at: 4811 4811 Size: 5294 SfcI C'TryA_G Cuts at: 33 472 1337 1400 2228 2337 3022 4015 4206 Size: 439 865 63 828 109 685 993 191 Cuts at: 4206 33 Size: 1121 Fragments arranged by size: 1121 993 865 828 685 439 191 109 63 SfoI GGC'GCC Cuts at: 2726 2974 2726 Size: 248 5046 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5294 SmaI CCC'GGG Cuts at: 2316 2316 Size: 5294 SmlI C'TyrA_G Cuts at: 865 2449 3364 3856 4118 865 Size: 1584 915 492 262 2041 Fragments arranged by size: 2041 1584 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 5294 SphI G_CATG'C Cuts at: 2347 2847 2347 Size: 500 4794 StuI AGG'CCT Cuts at: 1621 1621 Size: 5294 StyI C'CwwG_G Cuts at: 107 453 2428 2782 107 Size: 346 1975 354 2619 Fragments arranged by size: 2619 1975 354 346 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1418 2877 3010 3469 3678 1418 Size: 1459 133 459 209 3034 Fragments arranged by size: 3034 1459 459 209 133 TatI w'GTAC_w Cuts at: 4809 4809 Size: 5294 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4811 4843 4811 Size: 32 5262 Tth111I GACn'n_nGTC Cuts at: 3091 3091 Size: 5294 XbaI T'CTAG_A Cuts at: 2325 2325 Size: 5294 XcmI CCAnnnn_n'nnnnTGG Cuts at: 489 602 1042 2789 489 Size: 113 440 1747 2994 Fragments arranged by size: 2994 1747 440 113 XmaI C'CCGG_G Cuts at: 2314 2314 Size: 5294 XmnI GAAnn'nnTTC Cuts at: 527 527 Size: 5294 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AhdI AloI ApaI AscI AvrII BaeI BcgI BfrBI BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI DraIII EcoNI FseI FspAI HindIII NdeI NheI NotI NruI NsiI PacI PasI PfoI PmlI PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI