(Linear) (Six-base) MAPSORT of: KIBB4372.seq Check: 6310 from: 1 to: 2181 KIBB4372.orf With 182 enzymes: SgfI * November 16, 2012 10:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1288 2181 Size: 1288 893 AccI GT'mk_AC Cuts at: 0 650 2181 Size: 650 1531 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1085 1850 2181 Size: 1085 765 331 Fragments arranged by size: 1085 765 331 AleI CACnn'nnGTG Cuts at: 0 1642 2181 Size: 1642 539 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 147 939 2181 Size: 147 792 1242 Fragments arranged by size: 1242 792 147 AlwNI CAG_nnn'CTG Cuts at: 0 183 2181 Size: 183 1998 ApoI r'AATT_y Cuts at: 0 205 832 1585 1997 2047 2181 Size: 205 627 753 412 50 134 Fragments arranged by size: 753 627 412 205 134 50 BanII G_rGCy'C Cuts at: 0 600 1429 2181 Size: 600 829 752 Fragments arranged by size: 829 752 600 BbsI GAAGACnn'nnnn_ Cuts at: 0 769 2181 Size: 769 1412 BbvCI CC'TCA_GC Cuts at: 0 583 2181 Size: 583 1598 BglI GCCn_nnn'nGGC Cuts at: 0 1531 2181 Size: 1531 650 BlpI GC'TnA_GC Cuts at: 0 176 2181 Size: 176 2005 BmgBI CAC'GTC Cuts at: 0 840 1660 2181 Size: 840 820 521 Fragments arranged by size: 840 820 521 BmrI ACTGGGnnnn_n' Cuts at: 0 528 2181 Size: 528 1653 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 604 2181 Size: 604 1577 Bpu10I CC'TnA_GC Cuts at: 0 583 2181 Size: 583 1598 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 742 2181 Size: 742 1439 BsaBI GATnn'nnATC Cuts at: 0 1048 2181 Size: 1048 1133 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 632 662 1053 1083 2181 Size: 632 30 391 30 1098 Fragments arranged by size: 1098 632 391 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 56 242 245 2181 Size: 56 186 3 1936 Fragments arranged by size: 1936 186 56 3 BseYI C'CCAG_C Cuts at: 0 996 2181 Size: 996 1185 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1471 1620 2181 Size: 1471 149 561 Fragments arranged by size: 1471 561 149 BsiEI CG_ry'CG Cuts at: 0 1052 2181 Size: 1052 1129 BsiHKAI G_wGCw'C Cuts at: 0 51 2181 Size: 51 2130 Bsp1286I G_dGCh'C Cuts at: 0 51 600 1429 2181 Size: 51 549 829 752 Fragments arranged by size: 829 752 549 51 BspHI T'CATG_A Cuts at: 0 377 423 2181 Size: 377 46 1758 Fragments arranged by size: 1758 377 46 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 446 1288 2181 Size: 446 842 893 Fragments arranged by size: 893 842 446 BsrBI CCG'CTC Cuts at: 0 797 2181 Size: 797 1384 BsrDI GCAATG_nn' Cuts at: 0 469 692 2181 Size: 469 223 1489 Fragments arranged by size: 1489 469 223 BsrFI r'CCGG_y Cuts at: 0 2096 2181 Size: 2096 85 BstXI CCAn_nnnn'nTGG Cuts at: 0 738 2181 Size: 738 1443 BtgI C'CryG_G Cuts at: 0 345 866 2181 Size: 345 521 1315 Fragments arranged by size: 1315 521 345 BtsI GCAGTG_nn' Cuts at: 0 23 1637 2181 Size: 23 1614 544 Fragments arranged by size: 1614 544 23 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2138 2173 2181 Size: 2138 35 8 Fragments arranged by size: 2138 35 8 DraI TTT'AAA Cuts at: 0 1370 2181 Size: 1370 811 EaeI y'GGCC_r Cuts at: 0 1523 2181 Size: 1523 658 EarI CTCTTCn'nnn_ Cuts at: 0 1164 1882 2120 2136 2181 Size: 1164 718 238 16 45 Fragments arranged by size: 1164 718 238 45 16 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 604 1085 1850 2181 Size: 604 481 765 331 Fragments arranged by size: 765 604 481 331 EcoO109I rG'GnC_Cy Cuts at: 0 871 2181 Size: 871 1310 EcoRV GAT'ATC Cuts at: 0 213 361 1113 1269 2181 Size: 213 148 752 156 912 Fragments arranged by size: 912 752 213 156 148 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 436 2181 Size: 436 1745 FspI TGC'GCA Cuts at: 0 892 2181 Size: 892 1289 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 292 295 324 327 632 664 1163 1195 Size: 292 3 29 3 305 32 499 32 Cuts at: 1195 1210 1225 1242 1257 1302 1334 1644 1676 Size: 15 15 17 15 45 32 310 32 Cuts at: 1676 2181 Size: 505 Fragments arranged by size: 505 499 310 305 292 45 32 32 32 32 29 17 15 15 15 3 3 HincII GTy'rAC Cuts at: 0 150 1835 2181 Size: 150 1685 346 Fragments arranged by size: 1685 346 150 HpaI GTT'AAC Cuts at: 0 150 1835 2181 Size: 150 1685 346 Fragments arranged by size: 1685 346 150 MfeI C'AATT_G Cuts at: 0 558 644 2181 Size: 558 86 1537 Fragments arranged by size: 1537 558 86 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 18 365 1096 1545 2181 Size: 18 347 731 449 636 Fragments arranged by size: 731 636 449 347 18 MscI TGG'CCA Cuts at: 0 1525 2181 Size: 1525 656 MslI CAynn'nnrTG Cuts at: 0 350 428 671 1642 2181 Size: 350 78 243 971 539 Fragments arranged by size: 971 539 350 243 78 MspA1I CmG'CkG Cuts at: 0 2058 2181 Size: 2058 123 NaeI GCC'GGC Cuts at: 0 2098 2181 Size: 2098 83 NcoI C'CATG_G Cuts at: 0 345 2181 Size: 345 1836 NgoMIV G'CCGG_C Cuts at: 0 2096 2181 Size: 2096 85 NspI r_CATG'y Cuts at: 0 793 2181 Size: 793 1388 PflMI CCAn_nnn'nTGG Cuts at: 0 937 2181 Size: 937 1244 PpuMI rG'GwC_Cy Cuts at: 0 871 2181 Size: 871 1310 PsiI TTA'TAA Cuts at: 0 1757 2181 Size: 1757 424 PstI C_TGCA'G Cuts at: 0 368 1233 2124 2181 Size: 368 865 891 57 Fragments arranged by size: 891 865 368 57 PvuI CG_AT'CG Cuts at: 0 1052 2181 Size: 1052 1129 PvuII CAG'CTG Cuts at: 0 2058 2181 Size: 2058 123 SfcI C'TryA_G Cuts at: 0 364 1229 1292 2120 2181 Size: 364 865 63 828 61 Fragments arranged by size: 865 828 364 63 61 SmlI C'TyrA_G Cuts at: 0 757 2181 Size: 757 1424 StuI AGG'CCT Cuts at: 0 1513 2181 Size: 1513 668 StyI C'CwwG_G Cuts at: 0 345 2181 Size: 345 1836 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1310 2181 Size: 1310 871 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 381 494 934 2181 Size: 381 113 440 1247 Fragments arranged by size: 1247 440 381 113 XmnI GAAnn'nnTTC Cuts at: 0 419 2181 Size: 419 1762 Enzymes that do cut: AarI AccI AcuI AleI AlfI AlwNI ApoI BanII BbsI BbvCI BglI BlpI BmgBI BmrI BpmI Bpu10I BpuEI BsaBI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BstXI BtgI BtsI CspCI DraI EaeI EarI Eco57MI EcoO109I EcoRV FalI FspI Hin4I HincII HpaI MfeI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PflMI PpuMI PsiI PstI PvuI PvuII SfcI SmlI StuI StyI TaqII XcmI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI BfrBI BglII Bme1580I BmtI BplI BsaI BsaAI BsaHI BsaWI BsiWI BsmI BsmBI BspEI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI FseI FspAI HaeII HindIII KasI KpnI MluI NarI NdeI NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI Tth111I XbaI XhoI XmaI ZraI