Restriction map for pF1KB6490
##################################
# NO CUT ENZYMES (  146 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AvaI        AviII       AvrII       
BanI        BbeI        BbrPI       BcgI        BclI        
BcnI        BfaI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsoBI       BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrFI       BssHII      BssSI       
BstAPI      BstBI       BstEII      BstPI       BstUI       
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HapII       HhaI        Hin1I       
HinP1I      HindIII     HpaI        HpaII       KasI        
KpnI        KspI        MaeI        MamI        MfeI        
MluI        MroI        MspI        MunI        MvnI        
NaeI        NarI        NciI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    StuI        
SwaI        XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AfaI          837  GTAC                 GT^AC
AlwNI        1039  CAGGTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          114  CCTCAGG              CC^T[ACGT]AGG
Asp700I       120  GAATGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          242  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          351  TGGCCA               TGG^CCA
BamHI          35  GGATCC               G^GATCC
BanII         342  GAGCCC               G[GA]GC[CT]^C
BbsI          961  GTCTTC               GAAGAC(2/6)
BbvCI         885  CCTCAGC              CCTCAGC(-5/-2)
BciVI         267  GGATAC               GTATCC(6/5)
BglI          344  GCCCCTCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         961  GTCTTC               GAAGAC(2/6)
BsaAI         382  TACGTG               [CT]AC^GT[GA]
BseRI         781  CTCCTC               GAGGAG(10/8)
BsmFI         684  GGGAC                GGGAC(10/14)
BsmI          120  GAATGC               GAATGC(1/-1)
Bsp1407I      836  TGTACA               T^GTACA
BspMI         370  GCAGGT               ACCTGC(4/8)
BsrDI         564  CATTGC               GCAATG(2/0)
BsrGI         836  TGTACA               T^GTACA
Bst1107I      376  GTATAC               GTA^TAC
BstYI          35  GGATCC               [GA]^GATC[CT]
BstZ17I       376  GTATAC               GTA^TAC
Bsu36I        114  CCTCAGG              CC^T[ACGT]AGG
DraII        1040  AGGTCCT              [GA]G^G[ACGT]CC[CT]
DsaI          359  CCATGG               C^C[GA][CT]GG
EaeI          351  TGGCCA               [CT]^GGCC[GA]
EarI          136  CTCTTC               CTCTTC(1/4)
Eco81I        114  CCTCAGG              CC^T[ACGT]AGG
EcoNI        1032  CCTCGCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1040  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       359  CCATGG               C^C[AT][AT]GG
HgaI          246  GCGTC                GACGC(5/10)
HincII         42  GTCAAC               GT[CT]^[GA]AC
HindII         42  GTCAAC               GT[CT]^[GA]AC
HphI           60  GGTGA                GGTGA(8/7)
Ksp632I       136  CTCTTC               CTCTTC(1/4)
MflI           35  GGATCC               [GA]^GATC[CT]
MluNI         351  TGGCCA               TGG^CCA
MscI          351  TGGCCA               TGG^CCA
MspA1I        487  CAGCTG               C[AC]G^C[GT]G
MwoI          344  GCCCCTCTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI          359  CCATGG               C^CATGG
NspI          833  GCATGT               [GA]CATG^[CT]
PflFI         242  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        1040  AGGTCCT              [GA]G^G[AT]CC[CT]
PvuII         487  CAGCTG               CAG^CTG
RsaI          837  GTAC                 GT^AC
SspBI         836  TGTACA               T^GTACA
SspI          623  AATATT               AAT^ATT
StyI          359  CCATGG               C^C[AT][AT]GG
TaqI          953  TCGA                 T^CGA
Tsp509I        64  AATT                 ^AATT
TspRI        1014  CAGTG                CA[GC]TG[ACGT][ACGT]^
Tth111I       242  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       953  TCGA                 T^CGA
XhoII          35  GGATCC               [GA]^GATC[CT]
XmnI          120  GAATGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AccI          376  GTATAC               GT^[AC][GT]AC
              694  GTAGAC              
AlwI           36  GATCC                GGATC(4/5)
               35  GGATC               
BsgI          916  CTGCAC               GTGCAG(16/14)
              484  GTGCAG              
BsmAI         203  GAGAC                GTCTC(1/5)
              328  GAGAC               
BstXI          24  CCATGACTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              250  CCATACTGGTGG        
DpnII          36  GATC                 ^GATC
              105  GATC                
MboI           36  GATC                 ^GATC
              105  GATC                
MseI          176  TTAA                 T^TAA
              224  TTAA                
NdeII          36  GATC                 ^GATC
              105  GATC                
PflMI         354  CCATGCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1038  CCAGGTCCTGG         
Sau3AI         36  GATC                 ^GATC
              105  GATC                
SfaNI         599  GCATC                GCATC(5/9)
             1006  GCATC               
SfcI          279  CTACAG               C^T[GA][CT]AG
              435  CTACAG              
Tru9I         176  TTAA                 T^TAA
              224  TTAA                
Tsp45I        391  GTCAC                ^GT[GC]AC
              504  GTCAC               
Van91I        354  CCATGCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1038  CCAGGTCCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   37 enzymes) #
#########################################

AciI        AluI        AvaII       BbvI        BmyI        
BpmI        BsaJI       BsiYI       BslI        Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        EcoRII      Fnu4HI      FokI        
HaeIII      HinfI       ItaI        MaeII       MaeIII      
MboII       MnlI        MslI        MvaI        NlaIII      
NlaIV       PleI        Sau96I      ScrFI       SmlI        
TfiI        TseI        
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