Restriction map for pF1KB6490
 (Linear) (Six-base) MAPSORT of: KIBB6490.seq  Check: 7500  from: 1  to: 1071



KIBB6490.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     361    1071 
   Size:        361     710
 
AccI GT'mk_AC

Cuts at:      0     377     695    1071 
   Size:        377     318     376

  Fragments arranged by size: 

                 377     376     318
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142    1071 
   Size:        142     929
 
AleI CACnn'nnGTG

Cuts at:      0     292    1071 
   Size:        292     779
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0       2      34    1071 
   Size:          2      32    1037

  Fragments arranged by size: 

                1037      32       2
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1044    1071 
   Size:       1044      27
 
BamHI G'GATC_C

Cuts at:      0      35    1071 
   Size:         35    1036
 
BanII G_rGCy'C

Cuts at:      0     346    1071 
   Size:        346     725
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     954    1071 
   Size:        954     117
 
BbvCI CC'TCA_GC

Cuts at:      0     886    1071 
   Size:        886     185
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     261    1071 
   Size:        261     810
 
BglI GCCn_nnn'nGGC

Cuts at:      0     350    1071 
   Size:        350     721
 
Bme1580I G_kGCm'C

Cuts at:      0     299     367     799    1001    1071 
   Size:        299      68     432     202      70

  Fragments arranged by size: 

                 432     299     202      70      68
 
BmgBI CAC'GTC

Cuts at:      0     921    1071 
   Size:        921     150
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     697    1071 
   Size:        697     374
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     226    1071 
   Size:        226     845
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     569     733     813    1071 
   Size:         40     529     164      80     258

  Fragments arranged by size: 

                 529     258     164      80      40
 
Bpu10I CC'TnA_GC

Cuts at:      0     886    1071 
   Size:        886     185
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     601     664     769    1071 
   Size:        601      63     105     302

  Fragments arranged by size: 

                 601     302     105      63
 
BsaAI yAC'GTr

Cuts at:      0     384    1071 
   Size:        384     687
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      38      58      68      88    1071 
   Size:         38      20      10      20     983

  Fragments arranged by size: 

                 983      38      20      20      10
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     772    1071 
   Size:        772     299
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     505     901    1071 
   Size:        505     396     170

  Fragments arranged by size: 

                 505     396     170
 
BsmI GAATG_Cn'

Cuts at:      0     126    1071 
   Size:        126     945
 
Bsp1286I G_dGCh'C

Cuts at:      0     299     346     367     799    1001    1071 
   Size:        299      47      21     432     202      70

  Fragments arranged by size: 

                 432     299     202      70      47      21
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     361    1071 
   Size:        361     710
 
BsrDI GCAATG_nn'

Cuts at:      0     563    1071 
   Size:        563     508
 
BsrGI T'GTAC_A

Cuts at:      0     836    1071 
   Size:        836     235
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      31     257    1071 
   Size:         31     226     814

  Fragments arranged by size: 

                 814     226      31
 
BstYI r'GATC_y

Cuts at:      0      35    1071 
   Size:         35    1036
 
BstZ17I GTA'TAC

Cuts at:      0     378    1071 
   Size:        378     693
 
Bsu36I CC'TnA_GG

Cuts at:      0     115    1071 
   Size:        115     956
 
BtgI C'CryG_G

Cuts at:      0     359    1071 
   Size:        359     712
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     753     788    1071 
   Size:        753      35     283

  Fragments arranged by size: 

                 753     283      35
 
EaeI y'GGCC_r

Cuts at:      0     351    1071 
   Size:        351     720
 
EarI CTCTTCn'nnn_

Cuts at:      0     142    1071 
   Size:        142     929
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     871    1071 
   Size:        871     200
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     142     569     733     813    1071 
   Size:         40     102     427     164      80     258

  Fragments arranged by size: 

                 427     258     164     102      80      40
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1036    1071 
   Size:       1036      35
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1041    1071 
   Size:       1041      30
 
HincII GTy'rAC

Cuts at:      0      44    1071 
   Size:         44    1027
 
MscI TGG'CCA

Cuts at:      0     353    1071 
   Size:        353     718
 
MslI CAynn'nnrTG

Cuts at:      0      94     255     292    1071 
   Size:         94     161      37     779

  Fragments arranged by size: 

                 779     161      94      37
 
MspA1I CmG'CkG

Cuts at:      0     489    1071 
   Size:        489     582
 
NcoI C'CATG_G

Cuts at:      0     359    1071 
   Size:        359     712
 
NspI r_CATG'y

Cuts at:      0     837    1071 
   Size:        837     234
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     360    1044    1071 
   Size:        360     684      27

  Fragments arranged by size: 

                 684     360      27
 
PpuMI rG'GwC_Cy

Cuts at:      0    1041    1071 
   Size:       1041      30
 
PvuII CAG'CTG

Cuts at:      0     489    1071 
   Size:        489     582
 
SfcI C'TryA_G

Cuts at:      0     279     435    1071 
   Size:        279     156     636

  Fragments arranged by size: 

                 636     279     156
 
SmlI C'TyrA_G

Cuts at:      0     616     679     784    1071 
   Size:        616      63     105     287

  Fragments arranged by size: 

                 616     287     105      63
 
SspI AAT'ATT

Cuts at:      0     625    1071 
   Size:        625     446
 
StyI C'CwwG_G

Cuts at:      0     359    1071 
   Size:        359     712
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     679    1071 
   Size:        679     392
 
TatI w'GTAC_w

Cuts at:      0     836    1071 
   Size:        836     235
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      38      70    1071 
   Size:         38      32    1001

  Fragments arranged by size: 

                1001      38      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     245    1071 
   Size:        245     826
 
XmnI GAAnn'nnTTC

Cuts at:      0     124    1071 
   Size:        124     947
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AleI     AloI    AlwNI    BamHI    BanII
     BbsI    BbvCI    BciVI     BglI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaXI    BseRI     BsgI     BsmI
 Bsp1286I    BspMI    BsrDI    BsrGI    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    CspCI     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I
   HincII     MscI     MslI   MspA1I     NcoI     NspI    PflMI    PpuMI
    PvuII     SfcI     SmlI     SspI     StyI    TaqII     TatI     TstI
  Tth111I     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI     BanI     BbeI     BcgI     BclI    BfrBI    BglII
     BlpI     BmtI     BsaI    BsaBI    BsaHI    BsaWI    BseYI    BsiEI
  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrFI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BtgZI     BtsI     ClaI     DraI
   DraIII     DrdI     EagI  EcoICRI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI     XbaI     XcmI     XhoI
     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com