Restriction map for pF1KE0421
 (Linear) (Six-base) MAPSORT of: KIEE0421.seq  Check: 9032  from: 1  to: 567



KIEE0421.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     212     340     457     567 
   Size:        212     128     117     110

  Fragments arranged by size: 

                 212     128     117     110
 
AflIII A'CryG_T

Cuts at:      0      84     567 
   Size:         84     483
 
ApaLI G'TGCA_C

Cuts at:      0     285     567 
   Size:        285     282
 
ApoI r'AATT_y

Cuts at:      0     147     172     567 
   Size:        147      25     395

  Fragments arranged by size: 

                 395     147      25
 
BbvCI CC'TCA_GC

Cuts at:      0     485     567 
   Size:        485      82
 
BglI GCCn_nnn'nGGC

Cuts at:      0     195     567 
   Size:        195     372
 
Bme1580I G_kGCm'C

Cuts at:      0     289     567 
   Size:        289     278
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     383     536     567 
   Size:        383     153      31

  Fragments arranged by size: 

                 383     153      31
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     121     567 
   Size:        121     446
 
Bpu10I CC'TnA_GC

Cuts at:      0     485     567 
   Size:        485      82
 
BsaAI yAC'GTr

Cuts at:      0      19      85     567 
   Size:         19      66     482

  Fragments arranged by size: 

                 482      66      19
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     481     511     567 
   Size:        481      30      56

  Fragments arranged by size: 

                 481      56      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     502     567 
   Size:        502      65
 
BseYI C'CCAG_C

Cuts at:      0     254     567 
   Size:        254     313
 
BsiHKAI G_wGCw'C

Cuts at:      0     289     567 
   Size:        289     278
 
Bsp1286I G_dGCh'C

Cuts at:      0     289     567 
   Size:        289     278
 
BssHII G'CGCG_C

Cuts at:      0      49     567 
   Size:         49     518
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     310     567 
   Size:        310     257
 
BstYI r'GATC_y

Cuts at:      0      75     440     567 
   Size:         75     365     127

  Fragments arranged by size: 

                 365     127      75
 
BtgI C'CryG_G

Cuts at:      0      59     190     567 
   Size:         59     131     377

  Fragments arranged by size: 

                 377     131      59
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     314     349     567 
   Size:        314      35     218

  Fragments arranged by size: 

                 314     218      35
 
EaeI y'GGCC_r

Cuts at:      0     116     567 
   Size:        116     451
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     212     340     457     567 
   Size:        212     128     117     110

  Fragments arranged by size: 

                 212     128     117     110
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     335     367     481     513     567 
   Size:        335      32     114      32      54

  Fragments arranged by size: 

                 335     114      54      32      32
 
HincII GTy'rAC

Cuts at:      0     390     567 
   Size:        390     177
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     291     567 
   Size:        291     276
 
MslI CAynn'nnrTG

Cuts at:      0     112     567 
   Size:        112     455
 
MspA1I CmG'CkG

Cuts at:      0      61     258     331     435     567 
   Size:         61     197      73     104     132

  Fragments arranged by size: 

                 197     132     104      73      61
 
NcoI C'CATG_G

Cuts at:      0     190     567 
   Size:        190     377
 
NruI TCG'CGA

Cuts at:      0      96     567 
   Size:         96     471
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      36     567 
   Size:         36     531
 
PmlI CAC'GTG

Cuts at:      0      19     567 
   Size:         19     548
 
PvuII CAG'CTG

Cuts at:      0     258     331     435     567 
   Size:        258      73     104     132

  Fragments arranged by size: 

                 258     132     104      73
 
SacII CC_GC'GG

Cuts at:      0      62     567 
   Size:         62     505
 
StyI C'CwwG_G

Cuts at:      0     190     567 
   Size:        190     377
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      17      49     567 
   Size:         17      32     518

  Fragments arranged by size: 

                 518      32      17
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     262     567 
   Size:        262     305
 

 Enzymes that do cut:

     AcuI   AflIII    ApaLI     ApoI    BbvCI     BglI Bme1580I     BmrI
     BplI   Bpu10I    BsaAI    BsaXI    BseRI    BseYI  BsiHKAI Bsp1286I
   BssHII    BstXI    BstYI     BtgI    CspCI     EaeI  Eco57MI    Hin4I
   HincII     MmeI     MslI   MspA1I     NcoI     NruI    PflMI     PmlI
    PvuII    SacII     StyI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI
     BmtI     BpmI    BpuEI     BsaI    BsaBI    BsaHI    BsaWI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI     EagI
     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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