Restriction map for pF1KB9728
 (Linear) (Six-base) MAPSORT of: KIBB9728.seq  Check: 3391  from: 1  to: 1332



KIBB9728.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:50  ..

Acc65I G'GTAC_C

Cuts at:      0     948    1332 
   Size:        948     384
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     570    1332 
   Size:        570     762
 
AflIII A'CryG_T

Cuts at:      0     824    1332 
   Size:        824     508
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1073    1332 
   Size:       1073     259
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     547     579    1332 
   Size:        547      32     753

  Fragments arranged by size: 

                 753     547      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     387    1332 
   Size:        387     945
 
AvaI C'yCGr_G

Cuts at:      0     276     292     493     837     880    1332 
   Size:        276      16     201     344      43     452

  Fragments arranged by size: 

                 452     344     276     201      43      16
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     940     973    1332 
   Size:        940      33     359

  Fragments arranged by size: 

                 940     359      33
 
BanI G'GyrC_C

Cuts at:      0     244     259     415     684     696     772     867     948 
   Size:        244      15     156     269      12      76      95      81

Cuts at:    948    1030    1332 
   Size:         82     302

  Fragments arranged by size: 

                 302     269     244     156      95      82      81      76
                  15      12
 
BanII G_rGCy'C

Cuts at:      0      88     277     346     562    1006    1024    1332 
   Size:         88     189      69     216     444      18     308

  Fragments arranged by size: 

                 444     308     216     189      88      69      18
 
BbeI G_GCGC'C

Cuts at:      0     248     263     419     688     700     776    1034    1332 
   Size:        248      15     156     269      12      76     258     298

  Fragments arranged by size: 

                 298     269     258     248     156      76      15      12
            
 
BbvCI CC'TCA_GC

Cuts at:      0    1263    1332 
   Size:       1263      69
 
BglI GCCn_nnn'nGGC

Cuts at:      0     208     268     722    1090    1332 
   Size:        208      60     454     368     242

  Fragments arranged by size: 

                 454     368     242     208      60
 
BglII A'GATC_T

Cuts at:      0     374    1332 
   Size:        374     958
 
BlpI GC'TnA_GC

Cuts at:      0     367    1332 
   Size:        367     965
 
Bme1580I G_kGCm'C

Cuts at:      0     872    1332 
   Size:        872     460
 
BmgBI CAC'GTC

Cuts at:      0     816    1332 
   Size:        816     516
 
BmtI G_CTAG'C

Cuts at:      0    1271    1332 
   Size:       1271      61
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     150     576    1182    1332 
   Size:        150     426     606     150

  Fragments arranged by size: 

                 606     426     150     150
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52    1038    1089    1147    1187    1332 
   Size:         52     986      51      58      40     145

  Fragments arranged by size: 

                 986     145      58      52      51      40
 
Bpu10I CC'TnA_GC

Cuts at:      0    1263    1332 
   Size:       1263      69
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     592    1030    1332 
   Size:        592     438     302

  Fragments arranged by size: 

                 592     438     302
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     121    1332 
   Size:        121    1211
 
BsaHI Gr'CG_yC

Cuts at:      0     245     260     416     652     685     697     761     773 
   Size:        245      15     156     236      33      12      64      12

Cuts at:    773     887    1031    1066    1332 
   Size:        114     144      35     266

  Fragments arranged by size: 

                 266     245     236     156     144     114      64      35
                  33      15      12      12
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     547     577    1332 
   Size:        547      30     755

  Fragments arranged by size: 

                 755     547      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     126     129     132     174     568    1332 
   Size:        126       3       3      42     394     764

  Fragments arranged by size: 

                 764     394     126      42       3       3
 
BseYI C'CCAG_C

Cuts at:      0     349     778    1069    1332 
   Size:        349     429     291     263

  Fragments arranged by size: 

                 429     349     291     263
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      66    1084    1332 
   Size:         66    1018     248

  Fragments arranged by size: 

                1018     248      66
 
BsiEI CG_ry'CG

Cuts at:      0     577     899    1332 
   Size:        577     322     433

  Fragments arranged by size: 

                 577     433     322
 
BsiHKAI G_wGCw'C

Cuts at:      0     277     346    1024    1332 
   Size:        277      69     678     308

  Fragments arranged by size: 

                 678     308     277      69
 
BsmI GAATG_Cn'

Cuts at:      0     988    1332 
   Size:        988     344
 
Bsp1286I G_dGCh'C

Cuts at:      0      88     277     346     562     872    1006    1024    1332 
   Size:         88     189      69     216     310     134      18     308

  Fragments arranged by size: 

                 310     308     216     189     134      88      69      18
            
 
BsrBI CCG'CTC

Cuts at:      0      33     254     635    1332 
   Size:         33     221     381     697

  Fragments arranged by size: 

                 697     381     221      33
 
BsrFI r'CCGG_y

Cuts at:      0      24     572     763    1332 
   Size:         24     548     191     569

  Fragments arranged by size: 

                 569     548     191      24
 
BssHII G'CGCG_C

Cuts at:      0      62     394    1332 
   Size:         62     332     938

  Fragments arranged by size: 

                 938     332      62
 
BssSI C'ACGA_G

Cuts at:      0     999    1332 
   Size:        999     333
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     852    1332 
   Size:        852     480
 
BstYI r'GATC_y

Cuts at:      0     374    1332 
   Size:        374     958
 
Bsu36I CC'TnA_GG

Cuts at:      0     485    1332 
   Size:        485     847
 
BtgI C'CryG_G

Cuts at:      0     411     749     872     965    1332 
   Size:        411     338     123      93     367

  Fragments arranged by size: 

                 411     367     338     123      93
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     300    1332 
   Size:        300    1032
 
DraIII CAC_nnn'GTG

Cuts at:      0     627    1332 
   Size:        627     705
 
EaeI y'GGCC_r

Cuts at:      0     194     332     533     574     854     896     962    1332 
   Size:        194     138     201      41     280      42      66     370

  Fragments arranged by size: 

                 370     280     201     194     138      66      42      41
            
 
EagI C'GGCC_G

Cuts at:      0     574     896    1332 
   Size:        574     322     436

  Fragments arranged by size: 

                 574     436     322
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      25     147     556     606    1332 
   Size:         25     122     409      50     726

  Fragments arranged by size: 

                 726     409     122      50      25
 
EcoICRI GAG'CTC

Cuts at:      0     275     344    1022    1332 
   Size:        275      69     678     310

  Fragments arranged by size: 

                 678     310     275      69
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     570    1038    1089    1147    1187    1332 
   Size:         52     518     468      51      58      40     145

  Fragments arranged by size: 

                 518     468     145      58      52      51      40
 
EcoO109I rG'GnC_Cy

Cuts at:      0     489     877    1161    1332 
   Size:        489     388     284     171

  Fragments arranged by size: 

                 489     388     284     171
 
HaeII r_GCGC'y

Cuts at:      0     203     248     263     360     419     607     688     700 
   Size:        203      45      15      97      59     188      81      12

Cuts at:    700     776     977    1034    1332 
   Size:         76     201      57     298

  Fragments arranged by size: 

                 298     203     201     188      97      81      76      59
                  57      45      15      12
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1290    1322    1332 
   Size:       1290      32      10

  Fragments arranged by size: 

                1290      32      10
 
KasI G'GCGC_C

Cuts at:      0     244     259     415     684     696     772    1030    1332 
   Size:        244      15     156     269      12      76     258     302

  Fragments arranged by size: 

                 302     269     258     244     156      76      15      12
            
 
KpnI G_GTAC'C

Cuts at:      0     952    1332 
   Size:        952     380
 
MscI TGG'CCA

Cuts at:      0     334     856    1332 
   Size:        334     522     476

  Fragments arranged by size: 

                 522     476     334
 
MslI CAynn'nnrTG

Cuts at:      0     328     850    1332 
   Size:        328     522     482

  Fragments arranged by size: 

                 522     482     328
 
MspA1I CmG'CkG

Cuts at:      0     413     751     860     874    1332 
   Size:        413     338     109      14     458

  Fragments arranged by size: 

                 458     413     338     109      14
 
NaeI GCC'GGC

Cuts at:      0      26     574     765    1332 
   Size:         26     548     191     567

  Fragments arranged by size: 

                 567     548     191      26
 
NarI GG'CG_CC

Cuts at:      0     245     260     416     685     697     773    1031    1332 
   Size:        245      15     156     269      12      76     258     301

  Fragments arranged by size: 

                 301     269     258     245     156      76      15      12
            
 
NcoI C'CATG_G

Cuts at:      0     965    1332 
   Size:        965     367
 
NgoMIV G'CCGG_C

Cuts at:      0      24     572     763    1332 
   Size:         24     548     191     569

  Fragments arranged by size: 

                 569     548     191      24
 
NheI G'CTAG_C

Cuts at:      0    1267    1332 
   Size:       1267      65
 
NruI TCG'CGA

Cuts at:      0    1157    1332 
   Size:       1157     175
 
NspI r_CATG'y

Cuts at:      0     828    1113    1332 
   Size:        828     285     219

  Fragments arranged by size: 

                 828     285     219
 
PasI CC'CwG_GG

Cuts at:      0     666    1332 
   Size:        666     666
 
PciI A'CATG_T

Cuts at:      0     824    1332 
   Size:        824     508
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     627    1332 
   Size:        627     705
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     532     547     564     579    1332 
   Size:        532      15      17      15     753

  Fragments arranged by size: 

                 753     532      17      15      15
 
PpuMI rG'GwC_Cy

Cuts at:      0     877    1161    1332 
   Size:        877     284     171

  Fragments arranged by size: 

                 877     284     171
 
PstI C_TGCA'G

Cuts at:      0     392    1332 
   Size:        392     940
 
PvuII CAG'CTG

Cuts at:      0     860    1332 
   Size:        860     472
 
SacI G_AGCT'C

Cuts at:      0     277     346    1024    1332 
   Size:        277      69     678     308

  Fragments arranged by size: 

                 678     308     277      69
 
SacII CC_GC'GG

Cuts at:      0     414     752     875    1332 
   Size:        414     338     123     457

  Fragments arranged by size: 

                 457     414     338     123
 
SanDI GG'GwC_CC

Cuts at:      0     877    1332 
   Size:        877     455
 
SbfI CC_TGCA'GG

Cuts at:      0     392    1332 
   Size:        392     940
 
SfcI C'TryA_G

Cuts at:      0     388    1332 
   Size:        388     944
 
SfoI GGC'GCC

Cuts at:      0     246     261     417     686     698     774    1032    1332 
   Size:        246      15     156     269      12      76     258     300

  Fragments arranged by size: 

                 300     269     258     246     156      76      15      12
            
 
SgrAI Cr'CCGG_yG

Cuts at:      0     763    1332 
   Size:        763     569
 
SmaI CCC'GGG

Cuts at:      0     839     882    1332 
   Size:        839      43     450

  Fragments arranged by size: 

                 839     450      43
 
SmlI C'TyrA_G

Cuts at:      0     607    1045    1332 
   Size:        607     438     287

  Fragments arranged by size: 

                 607     438     287
 
StuI AGG'CCT

Cuts at:      0    1097    1332 
   Size:       1097     235
 
StyI C'CwwG_G

Cuts at:      0     965    1332 
   Size:        965     367
 
XmaI C'CCGG_G

Cuts at:      0     837     880    1332 
   Size:        837      43     452

  Fragments arranged by size: 

                 837     452      43
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     AlfI     AloI    AlwNI     AvaI     BaeI
     BanI    BanII     BbeI    BbvCI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BsrBI    BsrFI   BssHII    BssSI    BstXI    BstYI   Bsu36I     BtgI
    BtgZI   DraIII     EaeI     EagI     EciI  EcoICRI  Eco57MI EcoO109I
    HaeII    Hin4I     KasI     KpnI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NheI     NruI     NspI     PasI     PciI
    PflMI     PpiI    PpuMI     PstI    PvuII     SacI    SacII    SanDI
     SbfI     SfcI     SfoI    SgrAI     SmaI     SmlI     StuI     StyI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
     AleI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
    BamHI     BbsI     BcgI    BciVI     BclI    BfrBI     BmrI    BsaAI
    BsaBI    BsaWI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrDI
    BsrGI   BstAPI    BstBI   BstEII  BstZ17I     BtsI     ClaI    CspCI
     DraI     DrdI     EarI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     MfeI     MluI     MmeI
     NdeI     NotI     NsiI     PacI     PfoI     PmeI     PmlI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SalI     SapI
     ScaI    SexAI     SfiI     SgfI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmnI     ZraI
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