Restriction map for pF1KB7669
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       BalI        BamHI       BbrPI       
BbvCI       BcgI        BciVI       BclI        BfrI        
BglI        BglII       BsaAI       BsaBI       BsaWI       
BseAI       BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstF5I      BstXI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        DrdI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoRI       EcoRV       EcoT22I     
FbaI        FokI        FseI        FspI        HindIII     
KpnI        KspI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MslI        MunI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacII       SapI        SbfI        ScaI        
SexAI       SfaNI       SfiI        SfuI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TfiI        Tsp509I     
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccI          280  GTCGAC               GT^[AC][GT]AC
AcyI          946  GGCGCC               G[GA]^CG[CT]C
AfaI          897  GTAC                 GT^AC
AlwNI         253  CAGCCACTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         607  GAGCTC               G[AT]GC[AT]^C
AvaII         195  GGACC                G^G[AT]CC
AvrII         424  CCTAGG               C^CTAGG
BanI          946  GGCGCC               G^G[CT][GA]CC
BbeI          946  GGCGCC               GGCGC^C
BbsI           78  GTCTTC               GAAGAC(2/6)
BlnI          424  CCTAGG               C^CTAGG
BlpI          728  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      728  GCTCAGC              GC^T[ACGT]AGC
BpuAI          78  GTCTTC               GAAGAC(2/6)
BsaHI         946  GGCGCC               G[GA]^CG[CT]C
BsgI           42  GTGCAG               GTGCAG(16/14)
BsiHKAI       607  GAGCTC               G[AT]GC[AT]^C
BsmBI         442  GAGACG               CGTCTC(1/5)
BsmFI         243  GTCCC                GGGAC(10/14)
BspMI         919  ACCTGC               ACCTGC(4/8)
BstEII        171  GGTCACC              G^GT[ACGT]ACC
BstPI         171  GGTCACC              G^GT[ACGT]ACC
BstYI         668  AGATCC               [GA]^GATC[CT]
CelII         728  GCTCAGC              GC^T[ACGT]AGC
DraIII        845  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I       171  GGTCACC              G^GT[ACGT]ACC
HaeII         946  GGCGCC               [GA]GCGC^[CT]
Hin1I         946  GGCGCC               G[GA]^CG[CT]C
HpaI          886  GTTAAC               GTT^AAC
KasI          946  GGCGCC               G^GCGCC
MaeII         627  ACGT                 A^CGT
MflI          668  AGATCC               [GA]^GATC[CT]
MseI          887  TTAA                 T^TAA
NaeI          943  GCCGGC               GCC^GGC
NarI          946  GGCGCC               GG^CGCC
NcoI          233  CCATGG               C^CATGG
NgoMIV        943  GCCGGC               G^CCGGC
NspI          293  ACATGC               [GA]CATG^[CT]
PpuMI         194  AGGACCT              [GA]G^G[AT]CC[CT]
PvuII         756  CAGCTG               CAG^CTG
RsaI          897  GTAC                 GT^AC
SacI          607  GAGCTC               GAGCT^C
SalI          280  GTCGAC               G^TCGAC
SfcI          333  CTACAG               C^T[GA][CT]AG
SfoI          946  GGCGCC               GGC^GCC
SgrAI         942  CGCCGGCG             C[GA]^CCGG[CT]G
SmaI          688  CCCGGG               CCC^GGG
TaqI          281  TCGA                 T^CGA
Tru9I         887  TTAA                 T^TAA
TthHB8I       281  TCGA                 T^CGA
XhoII         668  AGATCC               [GA]^GATC[CT]
XmaCI         688  CCCGGG               C^CCGGG
XmaI          688  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AccII         177  CGCG                 CG^CG
              936  CGCG                
AlwI          669  GATCC                GGATC(4/5)
              982  GATCC               
ApaI          685  GGGCCC               GGGCC^C
              696  GGGCCC              
BfaI          425  CTAG                 C^TAG
              499  CTAG                
BsiEI         275  CGGTCG               CG[GA][CT]^CG
              540  CGACCG              
BstUI         177  CGCG                 CG^CG
              936  CGCG                
DdeI          729  CTCAG                C^T[ACGT]AG
              740  CTAAG               
DraII         194  AGGACCT              [GA]G^G[ACGT]CC[CT]
              684  GGGGCCC             
DsaI          167  CCACGG               C^C[GA][CT]GG
              233  CCATGG              
EcoO109I      194  AGGACCT              [GA]G^G[ACGT]CC[CT]
              684  GGGGCCC             
EcoT14I       233  CCATGG               C^C[AT][AT]GG
              424  CCTAGG              
HgaI          444  GACGC                GACGC(5/10)
              811  GACGC               
HincII        280  GTCGAC               GT[CT]^[GA]AC
              886  GTTAAC              
HindII        280  GTCGAC               GT[CT]^[GA]AC
              886  GTTAAC              
MaeI          425  CTAG                 C^TAG
              499  CTAG                
MaeIII        172  GTCAC                ^GT[ACGT]AC
              968  GTGAC               
MvnI          177  CGCG                 CG^CG
              936  CGCG                
StyI          233  CCATGG               C^C[AT][AT]GG
              424  CCTAGG              
Tsp45I        172  GTCAC                ^GT[GC]AC
              968  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AciI        AluI        AvaI        BanII       BbvI        
BcnI        BmyI        BpmI        BsaI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       BsoBI       
Bsp1286I    BsrFI       BsrI        BssKI       BstNI       
Cac8I       CfoI        Cfr10I      Cfr13I      DpnII       
EarI        EcoRII      FauI        Fnu4HI      HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MboI        MboII       
MnlI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
Sau3AI      Sau96I      ScrFI       TseI        TspRI       
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