Restriction map for pF1KB7669
 (Linear) (Six-base) MAPSORT of: KIBB7669.seq  Check: 2964  from: 1  to: 1014



KIBB7669.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     928    1014 
   Size:        928      86
 
AccI GT'mk_AC

Cuts at:      0     281    1014 
   Size:        281     733
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     738    1014 
   Size:        738     276
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     528    1014 
   Size:        528     486
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     982    1014 
   Size:        982      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     258    1014 
   Size:        258     756
 
ApaI G_GGCC'C

Cuts at:      0     689     700    1014 
   Size:        689      11     314

  Fragments arranged by size: 

                 689     314      11
 
AvaI C'yCGr_G

Cuts at:      0     139     413     434     688    1014 
   Size:        139     274      21     254     326

  Fragments arranged by size: 

                 326     274     254     139      21
 
AvrII C'CTAG_G

Cuts at:      0     424    1014 
   Size:        424     590
 
BanI G'GyrC_C

Cuts at:      0     946    1014 
   Size:        946      68
 
BanII G_rGCy'C

Cuts at:      0     420     611     689     700     730    1014 
   Size:        420     191      78      11      30     284

  Fragments arranged by size: 

                 420     284     191      78      30      11
 
BbeI G_GCGC'C

Cuts at:      0     950    1014 
   Size:        950      64
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      71    1014 
   Size:         71     943
 
BlpI GC'TnA_GC

Cuts at:      0     729    1014 
   Size:        729     285
 
Bme1580I G_kGCm'C

Cuts at:      0     167     689     700    1014 
   Size:        167     522      11     314

  Fragments arranged by size: 

                 522     314     167      11
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     234    1014 
   Size:        234     780
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184     532     610     652    1014 
   Size:        184     348      78      42     362

  Fragments arranged by size: 

                 362     348     184      78      42
 
Bpu10I CC'TnA_GC

Cuts at:      0     740    1014 
   Size:        740     274
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     142     423     936    1014 
   Size:        142     281     513      78

  Fragments arranged by size: 

                 513     281     142      78
 
BsaHI Gr'CG_yC

Cuts at:      0     947    1014 
   Size:        947      67
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      13      19      43      49     982    1012    1014 
   Size:         13       6      24       6     933      30       2

  Fragments arranged by size: 

                 933      30      24      13       6       6       2
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      39     454     478     994    1014 
   Size:         39     415      24     516      20

  Fragments arranged by size: 

                 516     415      39      24      20
 
BseYI C'CCAG_C

Cuts at:      0     266     408    1014 
   Size:        266     142     606

  Fragments arranged by size: 

                 606     266     142
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      63    1014 
   Size:         63     951
 
BsiEI CG_ry'CG

Cuts at:      0     278     543    1014 
   Size:        278     265     471

  Fragments arranged by size: 

                 471     278     265
 
BsiHKAI G_wGCw'C

Cuts at:      0     611    1014 
   Size:        611     403
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     436    1014 
   Size:        436     578
 
Bsp1286I G_dGCh'C

Cuts at:      0     167     420     611     689     700     730    1014 
   Size:        167     253     191      78      11      30     284

  Fragments arranged by size: 

                 284     253     191     167      78      30      11
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     928    1014 
   Size:        928      86
 
BsrFI r'CCGG_y

Cuts at:      0     133     521     735     943    1014 
   Size:        133     388     214     208      71

  Fragments arranged by size: 

                 388     214     208     133      71
 
BstEII G'GTnAC_C

Cuts at:      0     171    1014 
   Size:        171     843
 
BstYI r'GATC_y

Cuts at:      0     668    1014 
   Size:        668     346
 
BtgI C'CryG_G

Cuts at:      0     167     233    1014 
   Size:        167      66     781

  Fragments arranged by size: 

                 781     167      66
 
BtsI GCAGTG_nn'

Cuts at:      0     345     363     972    1014 
   Size:        345      18     609      42

  Fragments arranged by size: 

                 609     345      42      18
 
DraIII CAC_nnn'GTG

Cuts at:      0     850    1014 
   Size:        850     164
 
EarI CTCTTCn'nnn_

Cuts at:      0     234     455     674    1014 
   Size:        234     221     219     340

  Fragments arranged by size: 

                 340     234     221     219
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     359     540     568     721    1014 
   Size:        359     181      28     153     293

  Fragments arranged by size: 

                 359     293     181     153      28
 
EcoICRI GAG'CTC

Cuts at:      0     609    1014 
   Size:        609     405
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184     532     610     652     738    1014 
   Size:        184     348      78      42      86     276

  Fragments arranged by size: 

                 348     276     184      86      78      42
 
EcoO109I rG'GnC_Cy

Cuts at:      0     195     685    1014 
   Size:        195     490     329

  Fragments arranged by size: 

                 490     329     195
 
HaeII r_GCGC'y

Cuts at:      0     950    1014 
   Size:        950      64
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      13      19      45      51     577     609     819     851 
   Size:         13       6      26       6     526      32     210      32

Cuts at:    851    1014 
   Size:        163

  Fragments arranged by size: 

                 526     210     163      32      32      26      13       6
                   6
 
HincII GTy'rAC

Cuts at:      0     282     888    1014 
   Size:        282     606     126

  Fragments arranged by size: 

                 606     282     126
 
HpaI GTT'AAC

Cuts at:      0     888    1014 
   Size:        888     126
 
KasI G'GCGC_C

Cuts at:      0     946    1014 
   Size:        946      68
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     548     575    1014 
   Size:        548      27     439

  Fragments arranged by size: 

                 548     439      27
 
MspA1I CmG'CkG

Cuts at:      0     377     702     758     962    1014 
   Size:        377     325      56     204      52

  Fragments arranged by size: 

                 377     325     204      56      52
 
NaeI GCC'GGC

Cuts at:      0     945    1014 
   Size:        945      69
 
NarI GG'CG_CC

Cuts at:      0     947    1014 
   Size:        947      67
 
NcoI C'CATG_G

Cuts at:      0     233    1014 
   Size:        233     781
 
NgoMIV G'CCGG_C

Cuts at:      0     943    1014 
   Size:        943      71
 
NspI r_CATG'y

Cuts at:      0     297    1014 
   Size:        297     717
 
PasI CC'CwG_GG

Cuts at:      0     246    1014 
   Size:        246     768
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     982    1014 
   Size:        982      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     195    1014 
   Size:        195     819
 
PspOMI G'GGCC_C

Cuts at:      0     685     696    1014 
   Size:        685      11     318

  Fragments arranged by size: 

                 685     318      11
 
PvuII CAG'CTG

Cuts at:      0     758    1014 
   Size:        758     256
 
SacI G_AGCT'C

Cuts at:      0     611    1014 
   Size:        611     403
 
SalI G'TCGA_C

Cuts at:      0     280    1014 
   Size:        280     734
 
SfcI C'TryA_G

Cuts at:      0     333    1014 
   Size:        333     681
 
SfoI GGC'GCC

Cuts at:      0     948    1014 
   Size:        948      66
 
SgrAI Cr'CCGG_yG

Cuts at:      0     943    1014 
   Size:        943      71
 
SmaI CCC'GGG

Cuts at:      0     690    1014 
   Size:        690     324
 
SrfI GCCC'GGGC

Cuts at:      0     690    1014 
   Size:        690     324
 
StyI C'CwwG_G

Cuts at:      0     233     424    1014 
   Size:        233     191     590

  Fragments arranged by size: 

                 590     233     191
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     120     553     557    1014 
   Size:        120     433       4     457

  Fragments arranged by size: 

                 457     433     120       4
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     187     219    1014 
   Size:        187      32     795

  Fragments arranged by size: 

                 795     187      32
 
XmaI C'CCGG_G

Cuts at:      0     688    1014 
   Size:        688     326
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AlfI     AloI    AlwNI     ApaI     AvaI
    AvrII     BanI    BanII     BbeI     BbsI     BlpI Bme1580I     BmrI
     BpmI   Bpu10I     BsaI    BsaHI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrFI   BstEII    BstYI
     BtgI     BtsI   DraIII     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    HaeII    Hin4I   HincII     HpaI     KasI     MmeI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PasI     PpiI    PpuMI   PspOMI
    PvuII     SacI     SalI     SfcI     SfoI    SgrAI     SmaI     SrfI
     StyI    TaqII     TstI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI    ApaLI     ApoI     AscI     AseI    AsiSI     BaeI    BamHI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII    BmgBI
     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaWI    BsiWI     BsmI
    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
    BstBI    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
     DrdI     EaeI     EagI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI  HindIII     KpnI     MfeI     MluI     MscI     MslI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PciI    PflMI
     PfoI     PmeI     PmlI    PshAI     PsiI    PspXI     PsrI     PstI
     PvuI    RsrII    SacII    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SgfI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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