Restriction map for pF1KE0471
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AviII       
AvrII       BbrPI       BbvCI       BcgI        BciVI       
BclI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrDI       
BsrGI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     CelII       ClaI        
CpoI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoRV       
EcoT22I     FbaI        FseI        FspI        HgaI        
HindIII     HpaI        KpnI        KspI        MamI        
MfeI        MluI        MroI        MunI        NaeI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tth111I     XbaI        XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AcyI          991  GGCGCC               G[GA]^CG[CT]C
AfaI          156  GTAC                 GT^AC
Asp700I       330  GAATGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         237  GAGCAC               G[AT]GC[AT]^C
BalI         1017  TGGCCA               TGG^CCA
BamHI         816  GGATCC               G^GATCC
BanI          991  GGCGCC               G^G[CT][GA]CC
BbeI          991  GGCGCC               GGCGC^C
BbsI          664  GAAGAC               GAAGAC(2/6)
BfaI         1253  CTAG                 C^TAG
BpuAI         664  GAAGAC               GAAGAC(2/6)
BsaHI         991  GGCGCC               G[GA]^CG[CT]C
BsiHKAI       237  GAGCAC               G[AT]GC[AT]^C
BsmI          654  GAATGC               GAATGC(1/-1)
BsrBI        1073  CCGCTC               CCGCTC(-3/-3)
BsrFI        1155  GCCGGT               [GA]^CCGG[CT]
BssHII        161  GCGCGC               G^CGCGC
Cfr10I       1155  GCCGGT               [GA]^CCGG[CT]
DraI          318  TTTAAA               TTT^AAA
EaeI         1017  TGGCCA               [CT]^GGCC[GA]
EarI          187  GAAGAG               CTCTTC(1/4)
FauI         1072  CCCGC                CCCGC(4/6)
HaeII         991  GGCGCC               [GA]GCGC^[CT]
Hin1I         991  GGCGCC               G[GA]^CG[CT]C
HincII       1129  GTTGAC               GT[CT]^[GA]AC
HindII       1129  GTTGAC               GT[CT]^[GA]AC
KasI          991  GGCGCC               G^GCGCC
Ksp632I       187  GAAGAG               CTCTTC(1/4)
MaeI         1253  CTAG                 C^TAG
MaeII        1121  ACGT                 A^CGT
MaeIII         79  GTCAC                ^GT[ACGT]AC
MluNI        1017  TGGCCA               TGG^CCA
MscI         1017  TGGCCA               TGG^CCA
MslI           12  CATGGCGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        536  CCGCTG               C[AC]G^C[GT]G
NarI          991  GGCGCC               GG^CGCC
PflMI         644  CCAACAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI          156  GTAC                 GT^AC
SfcI          288  CTGTAG               C^T[GA][CT]AG
SfiI          525  GGCCCAGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          991  GGCGCC               GGC^GCC
SmaI          965  CCCGGG               CCC^GGG
SmlI         1182  CTCAAG               C^T[CT][GA]AG
TaqI          545  TCGA                 T^CGA
Tsp45I         79  GTCAC                ^GT[GC]AC
TthHB8I       545  TCGA                 T^CGA
Van91I        644  CCAACAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         965  CCCGGG               C^CCGGG
XmaI          965  CCCGGG               C^CCGGG
XmnI          330  GAATGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AccII         160  CGCG                 CG^CG
              162  CGCG                
AocI          820  CCTGAGG              CC^T[ACGT]AGG
              955  CCTCAGG             
AvaI          965  CCCGGG               C^[CT]CG[GA]G
             1214  CTCGGG              
BsgI           39  GTGCAG               GTGCAG(16/14)
              472  GTGCAG              
BsmFI         405  GTCCC                GGGAC(10/14)
              941  GTCCC               
BsoBI         965  CCCGGG               C^[CT]CG[GA]G
             1214  CTCGGG              
BstUI         160  CGCG                 CG^CG
              162  CGCG                
BstYI         500  AGATCC               [GA]^GATC[CT]
              816  GGATCC              
Bsu36I        820  CCTGAGG              CC^T[ACGT]AGG
              955  CCTCAGG             
Eco81I        820  CCTGAGG              CC^T[ACGT]AGG
              955  CCTCAGG             
EcoNI          24  CCTTCTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              207  CCTCATTGAGG         
EcoT14I        11  CCATGG               C^C[AT][AT]GG
              917  CCATGG              
MflI          500  AGATCC               [GA]^GATC[CT]
              816  GGATCC              
MvnI          160  CGCG                 CG^CG
              162  CGCG                
NcoI           11  CCATGG               C^CATGG
              917  CCATGG              
PleI         1132  GACTC                GAGTC(4/5)
             1000  GAGTC               
StyI           11  CCATGG               C^C[AT][AT]GG
              917  CCATGG              
TfiI          231  GAATC                G^A[AT]TC
             1303  GAATC               
XhoII         500  AGATCC               [GA]^GATC[CT]
              816  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        ApaI        
ApoI        AvaII       BanII       BbvI        BcnI        
BglI        BmyI        BpmI        BsaI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstXI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EcoO109I    EcoRI       EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MboI        MboII       MnlI        MseI        MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PpuMI       Sau3AI      Sau96I      ScrFI       
SfaNI       Tru9I       TseI        Tsp509I     TspRI       
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