Restriction map for pF1KE0471
 (Linear) (Six-base) MAPSORT of: KIEE0471.seq  Check: 107  from: 1  to: 1317



KIEE0471.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

ApaI G_GGCC'C

Cuts at:      0     154     528    1111    1317 
   Size:        154     374     583     206

  Fragments arranged by size: 

                 583     374     206     154
 
ApoI r'AATT_y

Cuts at:      0     253     334     541    1317 
   Size:        253      81     207     776

  Fragments arranged by size: 

                 776     253     207      81
 
AvaI C'yCGr_G

Cuts at:      0     965    1214    1317 
   Size:        965     249     103

  Fragments arranged by size: 

                 965     249     103
 
BamHI G'GATC_C

Cuts at:      0     816    1317 
   Size:        816     501
 
BanI G'GyrC_C

Cuts at:      0     991    1317 
   Size:        991     326
 
BanII G_rGCy'C

Cuts at:      0     154     528     985    1111    1317 
   Size:        154     374     457     126     206

  Fragments arranged by size: 

                 457     374     206     154     126
 
BbeI G_GCGC'C

Cuts at:      0     995    1317 
   Size:        995     322
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     671    1317 
   Size:        671     646
 
BglI GCCn_nnn'nGGC

Cuts at:      0     532     631     989    1317 
   Size:        532      99     358     328

  Fragments arranged by size: 

                 532     358     328      99
 
Bme1580I G_kGCm'C

Cuts at:      0     154     528     767    1111    1317 
   Size:        154     374     239     344     206

  Fragments arranged by size: 

                 374     344     239     206     154
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     157     444    1317 
   Size:        157     287     873

  Fragments arranged by size: 

                 873     287     157
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     297     367     705     725     732    1317 
   Size:         49     248      70     338      20       7     585

  Fragments arranged by size: 

                 585     338     248      70      49      20       7
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1167    1317 
   Size:       1167     150
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     115     287     631    1317 
   Size:        115     172     344     686

  Fragments arranged by size: 

                 686     344     172     115
 
BsaHI Gr'CG_yC

Cuts at:      0     992    1317 
   Size:        992     325
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      22      52     455     485    1317 
   Size:         22      30     403      30     832

  Fragments arranged by size: 

                 832     403      30      30      22
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     133     562     910    1274    1317 
   Size:        133     429     348     364      43

  Fragments arranged by size: 

                 429     364     348     133      43
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      60     493    1317 
   Size:         60     433     824

  Fragments arranged by size: 

                 824     433      60
 
BsiHKAI G_wGCw'C

Cuts at:      0     241    1317 
   Size:        241    1076
 
BsmI GAATG_Cn'

Cuts at:      0     660    1317 
   Size:        660     657
 
Bsp1286I G_dGCh'C

Cuts at:      0     154     241     528     767     985    1111    1317 
   Size:        154      87     287     239     218     126     206

  Fragments arranged by size: 

                 287     239     218     206     154     126      87
 
BsrBI CCG'CTC

Cuts at:      0    1075    1317 
   Size:       1075     242
 
BsrFI r'CCGG_y

Cuts at:      0    1155    1317 
   Size:       1155     162
 
BssHII G'CGCG_C

Cuts at:      0     161    1317 
   Size:        161    1156
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      12     434     690    1015    1317 
   Size:         12     422     256     325     302

  Fragments arranged by size: 

                 422     325     302     256      12
 
BstYI r'GATC_y

Cuts at:      0     500     816    1317 
   Size:        500     316     501

  Fragments arranged by size: 

                 501     500     316
 
Bsu36I CC'TnA_GG

Cuts at:      0     821     956    1317 
   Size:        821     135     361

  Fragments arranged by size: 

                 821     361     135
 
BtgI C'CryG_G

Cuts at:      0      11     659     917    1046    1317 
   Size:         11     648     258     129     271

  Fragments arranged by size: 

                 648     271     258     129      11
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      31    1317 
   Size:         31    1286
 
BtsI GCAGTG_nn'

Cuts at:      0     473     767    1317 
   Size:        473     294     550

  Fragments arranged by size: 

                 550     473     294
 
DraI TTT'AAA

Cuts at:      0     320    1317 
   Size:        320     997
 
EaeI y'GGCC_r

Cuts at:      0    1017    1317 
   Size:       1017     300
 
EarI CTCTTCn'nnn_

Cuts at:      0     182    1317 
   Size:        182    1135
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     364    1317 
   Size:        364     953
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     297     367     705     725     732    1317 
   Size:         49     248      70     338      20       7     585

  Fragments arranged by size: 

                 585     338     248      70      49      20       7
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      28     211    1317 
   Size:         28     183    1106

  Fragments arranged by size: 

                1106     183      28
 
EcoO109I rG'GnC_Cy

Cuts at:      0      68     135     524     940    1108    1209    1317 
   Size:         68      67     389     416     168     101     108

  Fragments arranged by size: 

                 416     389     168     108     101      68      67
 
EcoRI G'AATT_C

Cuts at:      0     253     334     541    1317 
   Size:        253      81     207     776

  Fragments arranged by size: 

                 776     253     207      81
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1119    1317 
   Size:       1119     198
 
HaeII r_GCGC'y

Cuts at:      0     995    1317 
   Size:        995     322
 
HincII GTy'rAC

Cuts at:      0    1131    1317 
   Size:       1131     186
 
KasI G'GCGC_C

Cuts at:      0     991    1317 
   Size:        991     326
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     818    1317 
   Size:        818     499
 
MscI TGG'CCA

Cuts at:      0    1019    1317 
   Size:       1019     298
 
MslI CAynn'nnrTG

Cuts at:      0      16    1317 
   Size:         16    1301
 
MspA1I CmG'CkG

Cuts at:      0     538    1317 
   Size:        538     779
 
NarI GG'CG_CC

Cuts at:      0     992    1317 
   Size:        992     325
 
NcoI C'CATG_G

Cuts at:      0      11     917    1317 
   Size:         11     906     400

  Fragments arranged by size: 

                 906     400      11
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     650    1317 
   Size:        650     667
 
PfoI T'CCnGG_A

Cuts at:      0     338     721    1317 
   Size:        338     383     596

  Fragments arranged by size: 

                 596     383     338
 
PpuMI rG'GwC_Cy

Cuts at:      0      68     135     940    1317 
   Size:         68      67     805     377

  Fragments arranged by size: 

                 805     377      68      67
 
PspOMI G'GGCC_C

Cuts at:      0     150     524    1107    1317 
   Size:        150     374     583     210

  Fragments arranged by size: 

                 583     374     210     150
 
SanDI GG'GwC_CC

Cuts at:      0     940    1317 
   Size:        940     377
 
SfcI C'TryA_G

Cuts at:      0     288    1317 
   Size:        288    1029
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     532    1317 
   Size:        532     785
 
SfoI GGC'GCC

Cuts at:      0     993    1317 
   Size:        993     324
 
SmaI CCC'GGG

Cuts at:      0     967    1317 
   Size:        967     350
 
SmlI C'TyrA_G

Cuts at:      0    1182    1317 
   Size:       1182     135
 
SrfI GCCC'GGGC

Cuts at:      0     967    1317 
   Size:        967     350
 
StyI C'CwwG_G

Cuts at:      0      11     917    1317 
   Size:         11     906     400

  Fragments arranged by size: 

                 906     400      11
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     817    1044    1317 
   Size:        817     227     273

  Fragments arranged by size: 

                 817     273     227
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      22      38      54      70     455     487    1317 
   Size:         22      16      16      16     385      32     830

  Fragments arranged by size: 

                 830     385      32      22      16      16      16
 
XmaI C'CCGG_G

Cuts at:      0     965    1317 
   Size:        965     352
 
XmnI GAAnn'nnTTC

Cuts at:      0     334    1317 
   Size:        334     983
 

 Enzymes that do cut:

     ApaI     ApoI     AvaI    BamHI     BanI    BanII     BbeI     BbsI
     BglI Bme1580I     BmrI     BpmI    BpuEI     BsaI    BsaHI    BsaXI
    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I    BsrBI    BsrFI   BssHII
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI     EaeI
     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII
   HincII     KasI     MmeI     MscI     MslI   MspA1I     NarI     NcoI
    PflMI     PfoI    PpuMI   PspOMI    SanDI     SfcI     SfiI     SfoI
     SmaI     SmlI     SrfI     StyI    TaqII     TstI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I
    BsaAI    BsaBI    BsaWI    BseYI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BspMI    BsrDI    BsrGI    BssSI   BstAPI    BstBI   BstEII
  BstZ17I     ClaI    CspCI   DraIII     DrdI     EagI  EcoICRI    EcoRV
     FseI     FspI    FspAI    Hin4I  HindIII     HpaI     KpnI     MfeI
     MluI     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI    PshAI
     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI     SapI     SbfI     ScaI    SexAI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SspI     StuI     SwaI     TatI  Tth111I
     XbaI     XcmI     XhoI     ZraI
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