Restriction map for pF1KF0156
 (Linear) (Six-base) MAPSORT of: FLJ00156.seq  Check: 1597  from: 1  to: 5574



FLJ00156.orf

 With 182 enzymes: SgfI * 

                        September 4, 2009 17:42  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     956    5574 
   Size:        956    4618
 
AatII G_ACGT'C

Cuts at:      0    4109    5150    5574 
   Size:       4109    1041     424

  Fragments arranged by size: 

                4109    1041     424
 
AccI GT'mk_AC

Cuts at:      0    1196    2308    3335    5574 
   Size:       1196    1112    1027    2239

  Fragments arranged by size: 

                2239    1196    1112    1027
 
Acc65I G'GTAC_C

Cuts at:      0     296    5574 
   Size:        296    5278
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     381     569     654    1117    1264    1387    1622    3613 
   Size:        381     188      85     463     147     123     235    1991

Cuts at:   3613    3929    4743    4764    5010    5574 
   Size:        316     814      21     246     564

  Fragments arranged by size: 

                1991     814     564     463     381     316     246     235
                 188     147     123      85      21
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1797    5574 
   Size:       1797    3777
 
AleI CACnn'nnGTG

Cuts at:      0     290     964    4552    4912    5092    5421    5574 
   Size:        290     674    3588     360     180     329     153

  Fragments arranged by size: 

                3588     674     360     329     290     180     153
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     982    5439    5574 
   Size:        982    4457     135

  Fragments arranged by size: 

                4457     982     135
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      27    5574 
   Size:         27    5547
 
AlwNI CAG_nnn'CTG

Cuts at:      0     376     831    1389    1399    1731    1900    1947    2430 
   Size:        376     455     558      10     332     169      47     483

Cuts at:   2430    2454    4602    4787    5214    5574 
   Size:         24    2148     185     427     360

  Fragments arranged by size: 

                2148     558     483     455     427     376     360     332
                 185     169      47      24      10
 
ApaI G_GGCC'C

Cuts at:      0    1256    5242    5281    5574 
   Size:       1256    3986      39     293

  Fragments arranged by size: 

                3986    1256     293      39
 
ApaLI G'TGCA_C

Cuts at:      0    1332    5574 
   Size:       1332    4242
 
ApoI r'AATT_y

Cuts at:      0     350    3874    4246    5574 
   Size:        350    3524     372    1328

  Fragments arranged by size: 

                3524    1328     372     350
 
AvaI C'yCGr_G

Cuts at:      0    2671    4950    5574 
   Size:       2671    2279     624

  Fragments arranged by size: 

                2671    2279     624
 
BamHI G'GATC_C

Cuts at:      0    1045    2831    5574 
   Size:       1045    1786    2743

  Fragments arranged by size: 

                2743    1786    1045
 
BanI G'GyrC_C

Cuts at:      0     296    1298    1342    1408    4470    5155    5574 
   Size:        296    1002      44      66    3062     685     419

  Fragments arranged by size: 

                3062    1002     685     419     296      66      44
 
BanII G_rGCy'C

Cuts at:      0     458     488     765    1256    1497    1545    1700    2294 
   Size:        458      30     277     491     241      48     155     594

Cuts at:   2294    2980    3852    4931    5242    5281    5464    5574 
   Size:        686     872    1079     311      39     183     110

  Fragments arranged by size: 

                1079     872     686     594     491     458     311     277
                 241     183     155     110      48      39      30
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     420    2316    2348    2369    2410    3155    3525    4705 
   Size:        420    1896      32      21      41     745     370    1180

Cuts at:   4705    5350    5574 
   Size:        645     224

  Fragments arranged by size: 

                1896    1180     745     645     420     370     224      41
                  32      21
 
BbvCI CC'TCA_GC

Cuts at:      0      43     658    1450    1666    1883    2065    2652    3068 
   Size:         43     615     792     216     217     182     587     416

Cuts at:   3068    4405    4603    5432    5574 
   Size:       1337     198     829     142

  Fragments arranged by size: 

                1337     829     792     615     587     416     217     216
                 198     182     142      43
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    4941    4975    5574 
   Size:       4941      34     599

  Fragments arranged by size: 

                4941     599      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    5074    5574 
   Size:       5074     500
 
BclI T'GATC_A

Cuts at:      0    3012    5574 
   Size:       3012    2562
 
BfrBI ATG'CAT

Cuts at:      0    2238    2761    5574 
   Size:       2238     523    2813

  Fragments arranged by size: 

                2813    2238     523
 
BglI GCCn_nnn'nGGC

Cuts at:      0     562    2676    4565    4955    5574 
   Size:        562    2114    1889     390     619

  Fragments arranged by size: 

                2114    1889     619     562     390
 
BglII A'GATC_T

Cuts at:      0     623     746    3497    5574 
   Size:        623     123    2751    2077

  Fragments arranged by size: 

                2751    2077     623     123
 
BlpI GC'TnA_GC

Cuts at:      0     487    4930    5574 
   Size:        487    4443     644

  Fragments arranged by size: 

                4443     644     487
 
Bme1580I G_kGCm'C

Cuts at:      0    1256    1336    1419    4475    4873    5242    5281    5574 
   Size:       1256      80      83    3056     398     369      39     293

  Fragments arranged by size: 

                3056    1256     398     369     293      83      80      39
            
 
BmgBI CAC'GTC

Cuts at:      0    1179    2346    4038    4209    5574 
   Size:       1179    1167    1692     171    1365

  Fragments arranged by size: 

                1692    1365    1179    1167     171
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     919    2190    3751    5460    5574 
   Size:        919    1271    1561    1709     114

  Fragments arranged by size: 

                1709    1561    1271     919     114
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2058    4101    5574 
   Size:       2058    2043    1473

  Fragments arranged by size: 

                2058    2043    1473
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     114     502     853    1303    1492    1612    1698    1745 
   Size:        114     388     351     450     189     120      86      47

Cuts at:   1745    1759    1771    2032    2476    2513    2934    3514    4071 
   Size:         14      12     261     444      37     421     580     557

Cuts at:   4071    5574 
   Size:       1503

  Fragments arranged by size: 

                1503     580     557     450     444     421     388     351
                 261     189     120     114      86      47      37      14
                  12
 
Bpu10I CC'TnA_GC

Cuts at:      0      43     195     658     688    1118    1450    1666    1883 
   Size:         43     152     463      30     430     332     216     217

Cuts at:   1883    2065    2652    2974    2979    3068    4405    4603    5432 
   Size:        182     587     322       5      89    1337     198     829

Cuts at:   5432    5458    5574 
   Size:         26     116

  Fragments arranged by size: 

                1337     829     587     463     430     332     322     217
                 216     198     182     152     116      89      43      30
                  26       5
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     703    1710    2533    5492    5574 
   Size:        703    1007     823    2959      82

  Fragments arranged by size: 

                2959    1007     823     703      82
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      77    1630    5574 
   Size:         77    1553    3944

  Fragments arranged by size: 

                3944    1553      77
 
BsaAI yAC'GTr

Cuts at:      0    4073    5003    5094    5574 
   Size:       4073     930      91     480

  Fragments arranged by size: 

                4073     930     480      91
 
BsaBI GATnn'nnATC

Cuts at:      0    5305    5574 
   Size:       5305     269
 
BsaHI Gr'CG_yC

Cuts at:      0    4106    5147    5574 
   Size:       4106    1041     427

  Fragments arranged by size: 

                4106    1041     427
 
BsaWI w'CCGG_w

Cuts at:      0    3983    5574 
   Size:       3983    1591
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1945    1975    3303    3333    5574 
   Size:       1945      30    1328      30    2241

  Fragments arranged by size: 

                2241    1945    1328      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1342    1675    1990    2551    3942    4730    5574 
   Size:       1342     333     315     561    1391     788     844

  Fragments arranged by size: 

                1391    1342     844     788     561     333     315
 
BseYI C'CCAG_C

Cuts at:      0     390     863    1324    1482    2120    2856    3104    3120 
   Size:        390     473     461     158     638     736     248      16

Cuts at:   3120    3586    3724    4500    4763    5280    5574 
   Size:        466     138     776     263     517     294

  Fragments arranged by size: 

                 776     736     638     517     473     466     461     390
                 294     263     248     158     138      16
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     223    1061    1913    2431    3084    3124    3262    3411 
   Size:        223     838     852     518     653      40     138     149

Cuts at:   3411    4231    4243    5040    5574 
   Size:        820      12     797     534

  Fragments arranged by size: 

                 852     838     820     797     653     534     518     223
                 149     138      40      12
 
BsiHKAI G_wGCw'C

Cuts at:      0     458     488    1336    1700    1748    2018    2180    2980 
   Size:        458      30     848     364      48     270     162     800

Cuts at:   2980    4072    4931    5181    5464    5574 
   Size:       1092     859     250     283     110

  Fragments arranged by size: 

                1092     859     848     800     458     364     283     270
                 250     162     110      48      30
 
BsmI GAATG_Cn'

Cuts at:      0     549    2143    5574 
   Size:        549    1594    3431

  Fragments arranged by size: 

                3431    1594     549
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1212    1862    2533    4199    5154    5574 
   Size:       1212     650     671    1666     955     420

  Fragments arranged by size: 

                1666    1212     955     671     650     420
 
Bsp1286I G_dGCh'C

Cuts at:      0     458     488     765    1256    1336    1419    1497    1545 
   Size:        458      30     277     491      80      83      78      48

Cuts at:   1545    1700    1748    2018    2180    2294    2980    3852    4072 
   Size:        155      48     270     162     114     686     872     220

Cuts at:   4072    4475    4873    4931    5181    5242    5281    5464    5574 
   Size:        403     398      58     250      61      39     183     110

  Fragments arranged by size: 

                 872     686     491     458     403     398     277     270
                 250     220     183     162     155     114     110      83
                  80      78      61      58      48      48      39      30
            
 
BspEI T'CCGG_A

Cuts at:      0    3983    5574 
   Size:       3983    1591
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     956    1384    3742    4145    5574 
   Size:        956     428    2358     403    1429

  Fragments arranged by size: 

                2358    1429     956     428     403
 
BsrBI CCG'CTC

Cuts at:      0    2529    5042    5574 
   Size:       2529    2513     532

  Fragments arranged by size: 

                2529    2513     532
 
BsrDI GCAATG_nn'

Cuts at:      0    2238    3744    5574 
   Size:       2238    1506    1830

  Fragments arranged by size: 

                2238    1830    1506
 
BsrGI T'GTAC_A

Cuts at:      0     710    1961    5574 
   Size:        710    1251    3613

  Fragments arranged by size: 

                3613    1251     710
 
BssSI C'ACGA_G

Cuts at:      0    3420    5574 
   Size:       3420    2154
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1731    1900    4357    5574 
   Size:       1731     169    2457    1217

  Fragments arranged by size: 

                2457    1731    1217     169
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     150     180     799     969    3966    4911    5574 
   Size:        150      30     619     170    2997     945     663

  Fragments arranged by size: 

                2997     945     663     619     170     150      30
 
BstYI r'GATC_y

Cuts at:      0     623     746    1045    2831    3497    3828    5073    5574 
   Size:        623     123     299    1786     666     331    1245     501

  Fragments arranged by size: 

                1786    1245     666     623     501     331     299     123
            
 
Bsu36I CC'TnA_GG

Cuts at:      0     319    1589    5574 
   Size:        319    1270    3985

  Fragments arranged by size: 

                3985    1270     319
 
BtgI C'CryG_G

Cuts at:      0    2899    3323    3719    3842    5554    5574 
   Size:       2899     424     396     123    1712      20

  Fragments arranged by size: 

                2899    1712     424     396     123      20
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1873    3471    5574 
   Size:       1873    1598    2103

  Fragments arranged by size: 

                2103    1873    1598
 
BtsI GCAGTG_nn'

Cuts at:      0    1111    1732    3348    4358    5574 
   Size:       1111     621    1616    1010    1216

  Fragments arranged by size: 

                1616    1216    1111    1010     621
 
ClaI AT'CG_AT

Cuts at:      0    2474    5574 
   Size:       2474    3100
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     618     653    5574 
   Size:        618      35    4921

  Fragments arranged by size: 

                4921     618      35
 
DraI TTT'AAA

Cuts at:      0    2087    3564    3891    5574 
   Size:       2087    1477     327    1683

  Fragments arranged by size: 

                2087    1683    1477     327
 
DraIII CAC_nnn'GTG

Cuts at:      0    2633    3255    4062    5574 
   Size:       2633     622     807    1512

  Fragments arranged by size: 

                2633    1512     807     622
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    3922    5329    5574 
   Size:       3922    1407     245

  Fragments arranged by size: 

                3922    1407     245
 
EaeI y'GGCC_r

Cuts at:      0      57    1430    1517    3279    3956    4566    4677    5216 
   Size:         57    1373      87    1762     677     610     111     539

Cuts at:   5216    5557    5574 
   Size:        341      17

  Fragments arranged by size: 

                1762    1373     677     610     539     341     111      87
                  57      17
 
EarI CTCTTCn'nnn_

Cuts at:      0     887    1093    2008    2049    2510    2736    4060    5386 
   Size:        887     206     915      41     461     226    1324    1326

Cuts at:   5386    5574 
   Size:        188

  Fragments arranged by size: 

                1326    1324     915     887     461     226     206     188
                  41
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    4040    5574 
   Size:       4040    1534
 
EcoICRI GAG'CTC

Cuts at:      0     456     486    1698    2978    4929    5462    5574 
   Size:        456      30    1212    1280    1951     533     112

  Fragments arranged by size: 

                1951    1280    1212     533     456     112      30
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     114     381     502     569     654     853    1117    1264 
   Size:        114     267     121      67      85     199     264     147

Cuts at:   1264    1303    1387    1492    1612    1622    1698    1745    1759 
   Size:         39      84     105     120      10      76      47      14

Cuts at:   1759    1771    2032    2476    2513    2934    3514    3613    3929 
   Size:         12     261     444      37     421     580      99     316

Cuts at:   3929    4071    4743    4764    5010    5574 
   Size:        142     672      21     246     564

  Fragments arranged by size: 

                 672     580     564     444     421     316     267     264
                 261     246     199     147     142     121     120     114
                 105      99      85      84      76      67      47      39
                  37      21      14      12      10
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2826    3478    4969    5272    5574 
   Size:       2826     652    1491     303     302

  Fragments arranged by size: 

                2826    1491     652     303     302
 
EcoO109I rG'GnC_Cy

Cuts at:      0     535     913    1479    1806    2688    2970    3267    3771 
   Size:        535     378     566     327     882     282     297     504

Cuts at:   3771    4236    4269    4607    4754    4815    5238    5277    5454 
   Size:        465      33     338     147      61     423      39     177

Cuts at:   5454    5487    5516    5574 
   Size:         33      29      58

  Fragments arranged by size: 

                 882     566     535     504     465     423     378     338
                 327     297     282     177     147      61      58      39
                  33      33      29
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     603    1282    1809    2116    2344    3214    5574 
   Size:        603     679     527     307     228     870    2360

  Fragments arranged by size: 

                2360     870     679     603     527     307     228
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1783    1815    2569    2601    2701    2733    3403    3435 
   Size:       1783      32     754      32     100      32     670      32

Cuts at:   3435    3772    3804    5574 
   Size:        337      32    1770

  Fragments arranged by size: 

                1783    1770     754     670     337     100      32      32
                  32      32      32
 
HindIII A'AGCT_T

Cuts at:      0    3567    4029    5574 
   Size:       3567     462    1545

  Fragments arranged by size: 

                3567    1545     462
 
KpnI G_GTAC'C

Cuts at:      0     300    5574 
   Size:        300    5274
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2234    2412    3383    3606    4832    5574 
   Size:       2234     178     971     223    1226     742

  Fragments arranged by size: 

                2234    1226     971     742     223     178
 
MscI TGG'CCA

Cuts at:      0      59    1519    3281    4568    4679    5218    5574 
   Size:         59    1460    1762    1287     111     539     356

  Fragments arranged by size: 

                1762    1460    1287     539     356     111      59
 
MslI CAynn'nnrTG

Cuts at:      0      31     290     950     964    2023    2183    3964    4552 
   Size:         31     259     660      14    1059     160    1781     588

Cuts at:   4552    4912    5092    5421    5574 
   Size:        360     180     329     153

  Fragments arranged by size: 

                1781    1059     660     588     360     329     259     180
                 160     153      31      14
 
MspA1I CmG'CkG

Cuts at:      0     360     657    1600    2430    3108    3721    4763    4784 
   Size:        360     297     943     830     678     613    1042      21

Cuts at:   4784    4853    5574 
   Size:         69     721

  Fragments arranged by size: 

                1042     943     830     721     678     613     360     297
                  69      21
 
NcoI C'CATG_G

Cuts at:      0    2899    3842    5554    5574 
   Size:       2899     943    1712      20

  Fragments arranged by size: 

                2899    1712     943      20
 
NsiI A_TGCA'T

Cuts at:      0    2240    2763    5574 
   Size:       2240     523    2811

  Fragments arranged by size: 

                2811    2240     523
 
NspI r_CATG'y

Cuts at:      0     955    3450    3750    4185    5574 
   Size:        955    2495     300     435    1389

  Fragments arranged by size: 

                2495    1389     955     435     300
 
PasI CC'CwG_GG

Cuts at:      0    1536    3767    4223    5484    5574 
   Size:       1536    2231     456    1261      90

  Fragments arranged by size: 

                2231    1536    1261     456      90
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     306     507     831    1389    1515    1727    1947    2822 
   Size:        306     201     324     558     126     212     220     875

Cuts at:   2822    5234    5574 
   Size:       2412     340

  Fragments arranged by size: 

                2412     875     558     340     324     306     220     212
                 201     126
 
PfoI T'CCnGG_A

Cuts at:      0    1355    1748    2009    2834    3824    5574 
   Size:       1355     393     261     825     990    1750

  Fragments arranged by size: 

                1750    1355     990     825     393     261
 
PmlI CAC'GTG

Cuts at:      0    4073    5003    5094    5574 
   Size:       4073     930      91     480

  Fragments arranged by size: 

                4073     930     480      91
 
PpuMI rG'GwC_Cy

Cuts at:      0     535    1479    1806    2688    3267    3771    4236    4754 
   Size:        535     944     327     882     579     504     465     518

Cuts at:   4754    4815    5574 
   Size:         61     759

  Fragments arranged by size: 

                 944     882     759     579     535     518     504     465
                 327      61
 
PspOMI G'GGCC_C

Cuts at:      0    1252    5238    5277    5574 
   Size:       1252    3986      39     297

  Fragments arranged by size: 

                3986    1252     297      39
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    3745    3777    5574 
   Size:       3745      32    1797

  Fragments arranged by size: 

                3745    1797      32
 
PstI C_TGCA'G

Cuts at:      0     474     941    1226    1295    1445    2174    3482    3665 
   Size:        474     467     285      69     150     729    1308     183

Cuts at:   3665    4022    4515    4598    5381    5541    5574 
   Size:        357     493      83     783     160      33

  Fragments arranged by size: 

                1308     783     729     493     474     467     357     285
                 183     160     150      83      69      33
 
PvuII CAG'CTG

Cuts at:      0     360     657    1600    2430    3108    4763    4784    5574 
   Size:        360     297     943     830     678    1655      21     790

  Fragments arranged by size: 

                1655     943     830     790     678     360     297      21
            
 
SacI G_AGCT'C

Cuts at:      0     458     488    1700    2980    4931    5464    5574 
   Size:        458      30    1212    1280    1951     533     110

  Fragments arranged by size: 

                1951    1280    1212     533     458     110      30
 
SacII CC_GC'GG

Cuts at:      0    3722    5574 
   Size:       3722    1852
 
SanDI GG'GwC_CC

Cuts at:      0    4236    5574 
   Size:       4236    1338
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1093    4060    5574 
   Size:       1093    2967    1514

  Fragments arranged by size: 

                2967    1514    1093
 
SbfI CC_TGCA'GG

Cuts at:      0     941    3482    5574 
   Size:        941    2541    2092

  Fragments arranged by size: 

                2541    2092     941
 
SexAI A'CCwGG_T

Cuts at:      0    3251    3977    5574 
   Size:       3251     726    1597

  Fragments arranged by size: 

                3251    1597     726
 
SfcI C'TryA_G

Cuts at:      0     470     937    1222    1291    1441    2170    2733    3478 
   Size:        470     467     285      69     150     729     563     745

Cuts at:   3478    3661    4018    4511    4587    4594    4712    5377    5469 
   Size:        183     357     493      76       7     118     665      92

Cuts at:   5469    5537    5574 
   Size:         68      37

  Fragments arranged by size: 

                 745     729     665     563     493     470     467     357
                 285     183     150     118      92      76      69      68
                  37       7
 
SmaI CCC'GGG

Cuts at:      0    2673    5574 
   Size:       2673    2901
 
SmlI C'TyrA_G

Cuts at:      0     718    1689    2548    5507    5574 
   Size:        718     971     859    2959      67

  Fragments arranged by size: 

                2959     971     859     718      67
 
SphI G_CATG'C

Cuts at:      0     955    4185    5574 
   Size:        955    3230    1389

  Fragments arranged by size: 

                3230    1389     955
 
SrfI GCCC'GGGC

Cuts at:      0    2673    5574 
   Size:       2673    2901
 
StuI AGG'CCT

Cuts at:      0     331    2554    3559    4006    4975    5574 
   Size:        331    2223    1005     447     969     599

  Fragments arranged by size: 

                2223    1005     969     599     447     331
 
StyI C'CwwG_G

Cuts at:      0     526    1514    1640    2684    2899    3842    3857    5554 
   Size:        526     988     126    1044     215     943      15    1697

Cuts at:   5554    5574 
   Size:         20

  Fragments arranged by size: 

                1697    1044     988     943     526     215     126      20
                  15
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     234     691    1041    1954    3118    3958    4015    4514 
   Size:        234     457     350     913    1164     840      57     499

Cuts at:   4514    4586    5104    5574 
   Size:         72     518     470

  Fragments arranged by size: 

                1164     913     840     518     499     470     457     350
                 234      72      57
 
TatI w'GTAC_w

Cuts at:      0     710     989    1961    5574 
   Size:        710     279     972    3613

  Fragments arranged by size: 

                3613     972     710     279
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1037    1069    1409    1441    1471    1503    1569    1601 
   Size:       1037      32     340      32      30      32      66      32

Cuts at:   1601    2680    2712    3303    3335    3340    3372    5574 
   Size:       1079      32     591      32       5      32    2202

  Fragments arranged by size: 

                2202    1079    1037     591     340      66      32      32
                  32      32      32      32      32      30       5
 
Tth111I GACn'n_nGTC

Cuts at:      0    2059    4285    4813    5574 
   Size:       2059    2226     528     761

  Fragments arranged by size: 

                2226    2059     761     528
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1552    1840    3764    5434    5574 
   Size:       1552     288    1924    1670     140

  Fragments arranged by size: 

                1924    1670    1552     288     140
 
XmaI C'CCGG_G

Cuts at:      0    2671    5574 
   Size:       2671    2903
 
XmnI GAAnn'nnTTC

Cuts at:      0     515    3820    5574 
   Size:        515    3305    1754

  Fragments arranged by size: 

                3305    1754     515
 
ZraI GAC'GTC

Cuts at:      0    4107    5148    5574 
   Size:       4107    1041     426

  Fragments arranged by size: 

                4107    1041     426
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI     AhdI     AleI     AlfI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI    BamHI     BanI
    BanII     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspMI
    BsrBI    BsrDI    BsrGI    BssSI   BstAPI    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI
    Hin4I  HindIII     KpnI     MmeI     MscI     MslI   MspA1I     NcoI
     NsiI     NspI     PasI    PflMI     PfoI     PmlI    PpuMI   PspOMI
     PsrI     PstI    PvuII     SacI    SacII    SanDI     SapI     SbfI
    SexAI     SfcI     SmaI     SmlI     SphI     SrfI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AclI     AfeI    AflII   AflIII     AgeI     AscI     AseI    AsiSI
    AvrII     BaeI     BbeI     BmtI    BsiEI    BsiWI    BspHI    BsrFI
   BssHII    BstBI   BstEII  BstZ17I     EagI    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII   HincII     HpaI     KasI     MfeI     MluI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     PacI
     PciI     PmeI     PpiI    PshAI     PsiI    PspXI     PvuI    RsrII
     SalI     ScaI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SspI     SwaI     XbaI     XhoI
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