Restriction map for pF1KF0141
##################################
# NO CUT ENZYMES (   80 enzymes) #
##################################

AatII       AccI        AclI        AcsI        AgeI        
Alw44I      ApaLI       ApoI        AscI        AseI        
AsnI        AspI        AvrII       BamHI       BcgI        
BciVI       BclI        BglII       BlnI        BsaBI       
BsiEI       BsiWI       Bsp1407I    BspDI       BsrGI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     ClaI        CpoI        DraI        DrdI        
EagI        EclXI       Eco52I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        HindIII     HpaI        
KspI        MamI        MfeI        MluI        MunI        
NdeI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SbfI        ScaI        SfuI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

Acc65I        852  GGTACC               G^GTACC
AcyI          718  GGCGCC               G[GA]^CG[CT]C
AflII        1377  CTTAAG               C^TTAAG
AflIII       3266  ACATGT               A^C[GA][CT]GT
AhdI         3262  GACAACATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         1891  CCTGAGG              CC^T[ACGT]AGG
Asp700I      1280  GAACATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       852  GGTACC               G^GTACC
AspEI        3262  GACAACATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        3803  TGCGCA               TGC^GCA
BbeI          718  GGCGCC               GGCGC^C
BfrI         1377  CTTAAG               C^TTAAG
BsaHI         718  GGCGCC               G[GA]^CG[CT]C
BsmBI        3742  GAGACG               CGTCTC(1/5)
BspHI        3950  TCATGA               T^CATGA
BspLU11I     3266  ACATGT               A^CATGT
BsrDI         299  GCAATG               GCAATG(2/0)
BssSI        1961  CACGAG               CACGAG(-5/-1)
BstXI         722  CCATGTCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1891  CCTGAGG              CC^T[ACGT]AGG
Eam1105I     3262  GACAACATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1891  CCTGAGG              CC^T[ACGT]AGG
FseI         2475  GGCCGGCC             GGCCGG^CC
FspI         3803  TGCGCA               TGC^GCA
Hin1I         718  GGCGCC               G[GA]^CG[CT]C
HincII       1118  GTCAAC               GT[CT]^[GA]AC
HindII       1118  GTCAAC               GT[CT]^[GA]AC
KasI          718  GGCGCC               G^GCGCC
KpnI          852  GGTACC               GGTAC^C
NarI          718  GGCGCC               GG^CGCC
NheI         2139  GCTAGC               G^CTAGC
RcaI         3950  TCATGA               T^CATGA
SexAI         122  ACCTGGT              A^CC[AT]GGT
SfiI         1339  GGCCGGCGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          718  GGCGCC               GGC^GCC
SgrAI        1777  CACCGGCG             C[GA]^CCGG[CT]G
SmaI         2609  CCCGGG               CCC^GGG
Tsp509I      3503  AATT                 ^AATT
XmaCI        2609  CCCGGG               C^CCGGG
XmaI         2609  CCCGGG               C^CCGGG
XmnI         1280  GAACATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccIII       2492  TCCGGA               T^CCGGA
             3542  TCCGGA              
BalI         1076  TGGCCA               TGG^CCA
             2543  TGGCCA              
BbrPI        2176  CACGTG               CAC^GTG
             3610  CACGTG              
BbsI         2712  GAAGAC               GAAGAC(2/6)
             3564  GAAGAC              
BbvCI        3040  GCTGAGG              CCTCAGC(-5/-2)
             3931  GCTGAGG             
BpuAI        2712  GAAGAC               GAAGAC(2/6)
             3564  GAAGAC              
BsaAI        2176  CACGTG               [CT]AC^GT[GA]
             3610  CACGTG              
BsaWI        2492  TCCGGA               [AT]^CCGG[AT]
             3542  TCCGGA              
BseAI        2492  TCCGGA               T^CCGGA
             3542  TCCGGA              
BsmI         2619  GAATGC               GAATGC(1/-1)
             1130  GCATTC              
BspEI        2492  TCCGGA               T^CCGGA
             3542  TCCGGA              
BstYI        1434  AGATCC               [GA]^GATC[CT]
             2570  AGATCC              
EcoNI        2727  CCTCAAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3396  CCTGGAGGAGG         
HgaI            4  GACGC                GACGC(5/10)
             1521  GACGC               
MaeII        2177  ACGT                 A^CGT
             3611  ACGT                
MflI         1434  AGATCC               [GA]^GATC[CT]
             2570  AGATCC              
MluNI        1076  TGGCCA               TGG^CCA
             2543  TGGCCA              
MroI         2492  TCCGGA               T^CCGGA
             3542  TCCGGA              
MscI         1076  TGGCCA               TGG^CCA
             2543  TGGCCA              
NaeI         1340  GCCGGC               GCC^GGC
             2476  GCCGGC              
NgoMIV       1340  GCCGGC               G^CCGGC
             2476  GCCGGC              
NspI          359  GCATGT               [GA]CATG^[CT]
             3266  ACATGT              
PmaCI        2176  CACGTG               CAC^GTG
             3610  CACGTG              
PmlI         2176  CACGTG               CAC^GTG
             3610  CACGTG              
TfiI          432  GATTC                G^A[AT]TC
              484  GAATC               
XhoII        1434  AGATCC               [GA]^GATC[CT]
             2570  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (  111 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       Aor51HI     ApaI        AspHI       AvaI        
AvaII       BanI        BanII       BbvI        BcnI        
BfaI        BglI        BlpI        BmyI        BpmI        
Bpu1102I    BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrFI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DraIII      
DsaI        EaeI        EarI        Eco47III    EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StuI        StyI        TaqI        Tru9I       
TseI        Tsp45I      TspRI       TthHB8I     Van91I      
XcmI        
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