Restriction map for pF1KB6365
##################################
# NO CUT ENZYMES (  154 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AviII       AvrII       
BalI        BbrPI       BcgI        BciVI       BclI        
BfrI        BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BsiEI       
BsiHKAI     BsiWI       BsmFI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstZ17I     Bsu36I      Cac8I       CelII       
ClaI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
HphI        KpnI        KspI        MaeII       MamI        
MfeI        MluI        MluNI       MroI        MscI        
MunI        MvnI        NaeI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsaI        SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        SwaI        
TaqI        Tsp45I      Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AcsI          418  AAATTT               [GA]^AATT[CT]
AcyI          254  GGCGCC               G[GA]^CG[CT]C
AlwNI          48  CAGCGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       154  AGCGCT               AGC^GCT
ApoI          418  AAATTT               [GA]^AATT[CT]
BamHI         331  GGATCC               G^GATCC
BanI          254  GGCGCC               G^G[CT][GA]CC
BbeI          254  GGCGCC               GGCGC^C
BbsI          162  GTCTTC               GAAGAC(2/6)
BglI          257  GCCGGACCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         305  AGATCT               A^GATCT
BpmI          129  CTCCAG               CTGGAG(16/14)
BpuAI         162  GTCTTC               GAAGAC(2/6)
BsaHI         254  GGCGCC               G[GA]^CG[CT]C
BsmBI          73  CGTCTC               CGTCTC(1/5)
BsrFI         262  ACCGGC               [GA]^CCGG[CT]
BsrI          237  CCAGT                ACTGG(1/-1)
BstF5I        315  GGATG                GGATG(2/0)
BstNI          53  CCTGG                CC^[AT]GG
BstXI         207  CCAAGGAAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        262  ACCGGC               [GA]^CCGG[CT]
CpoI          259  CGGACCG              CG^G[AT]CCG
Eco47III      154  AGCGCT               AGC^GCT
EcoRII         53  CCTGG                ^CC[AT]GG
EcoT14I       207  CCAAGG               C^C[AT][AT]GG
FokI          315  GGATG                GGATG(9/13)
HaeIII        570  GGCC                 GG^CC
HgaI           72  GCGTC                GACGC(5/10)
Hin1I         254  GGCGCC               G[GA]^CG[CT]C
KasI          254  GGCGCC               G^GCGCC
MaeIII        691  GTTAC                ^GT[ACGT]AC
MslI          451  CATGCAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        101  CCGCTG               C[AC]G^C[GT]G
MvaI           53  CCTGG                CC^[AT]GG
NarI          254  GGCGCC               GG^CGCC
NlaIII        451  CATG                 CATG^
PleI          785  GACTC                GAGTC(4/5)
RsrII         259  CGGACCG              CG^G[AT]CCG
SfaNI         287  GCATC                GCATC(5/9)
SfoI          254  GGCGCC               GGC^GCC
SmaI          611  CCCGGG               CCC^GGG
SmlI          787  CTCAAG               C^T[CT][GA]AG
SspI          366  AATATT               AAT^ATT
StyI          207  CCAAGG               C^C[AT][AT]GG
Tsp509I       419  AATT                 ^AATT
XmaCI         611  CCCGGG               C^CCGGG
XmaI          611  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AvaI          249  CTCGGG               C^[CT]CG[GA]G
              611  CCCGGG              
AvaII          97  GGACC                G^G[AT]CC
              260  GGACC               
BanII          56  GGGCTC               G[GA]GC[CT]^C
              243  GAGCCC              
BbvCI          45  CCTCAGC              CCTCAGC(-5/-2)
              524  CCTCAGC             
BfaI          122  CTAG                 C^TAG
              719  CTAG                
BmyI           56  GGGCTC               G[AGT]GC[ACT]^C
              243  GAGCCC              
BsgI          749  CTGCAC               GTGCAG(16/14)
              603  GTGCAG              
BsmAI          74  GTCTC                GTCTC(1/5)
              378  GTCTC               
BsoBI         249  CTCGGG               C^[CT]CG[GA]G
              611  CCCGGG              
Bsp1286I       56  GGGCTC               G[AGT]GC[ACT]^C
              243  GAGCCC              
BstYI         305  AGATCT               [GA]^GATC[CT]
              331  GGATCC              
EarI           25  CTCTTC               CTCTTC(1/4)
              168  CTCTTC              
Ksp632I        25  CTCTTC               CTCTTC(1/4)
              168  CTCTTC              
MaeI          122  CTAG                 C^TAG
              719  CTAG                
MflI          305  AGATCT               [GA]^GATC[CT]
              331  GGATCC              
MseI          352  TTAA                 T^TAA
              617  TTAA                
PstI          276  CTGCAG               CTGCA^G
              301  CTGCAG              
SfcI          276  CTGCAG               C^T[GA][CT]AG
              301  CTGCAG              
TfiI          653  GATTC                G^A[AT]TC
              738  GATTC               
Tru9I         352  TTAA                 T^TAA
              617  TTAA                
XhoII         305  AGATCT               [GA]^GATC[CT]
              331  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   36 enzymes) #
#########################################

AciI        AluI        AlwI        BbvI        BcnI        
BsaJI       BseRI       BsiYI       BslI        BssKI       
CfoI        Cfr13I      DdeI        DpnII       FauI        
Fnu4HI      HaeII       HapII       HhaI        HinP1I      
HinfI       HpaII       ItaI        MboI        MboII       
MnlI        MspI        MwoI        NciI        NdeII       
NlaIV       Sau3AI      Sau96I      ScrFI       TseI        
TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com