Restriction map for pF1KB6365
 (Linear) (Six-base) MAPSORT of: KIBB6365.seq  Check: 3944  from: 1  to: 804



KIBB6365.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      15     804 
   Size:         15     789
 
AfeI AGC'GCT

Cuts at:      0     156     804 
   Size:        156     648
 
AlwNI CAG_nnn'CTG

Cuts at:      0      53     804 
   Size:         53     751
 
ApoI r'AATT_y

Cuts at:      0     418     804 
   Size:        418     386
 
AvaI C'yCGr_G

Cuts at:      0     249     611     804 
   Size:        249     362     193

  Fragments arranged by size: 

                 362     249     193
 
BamHI G'GATC_C

Cuts at:      0     331     804 
   Size:        331     473
 
BanI G'GyrC_C

Cuts at:      0     254     804 
   Size:        254     550
 
BanII G_rGCy'C

Cuts at:      0      60     247     804 
   Size:         60     187     557

  Fragments arranged by size: 

                 557     187      60
 
BbeI G_GCGC'C

Cuts at:      0     258     804 
   Size:        258     546
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     155     804 
   Size:        155     649
 
BbvCI CC'TCA_GC

Cuts at:      0      46     525     804 
   Size:         46     479     279

  Fragments arranged by size: 

                 479     279      46
 
BglI GCCn_nnn'nGGC

Cuts at:      0     263     804 
   Size:        263     541
 
BglII A'GATC_T

Cuts at:      0     305     804 
   Size:        305     499
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     114     804 
   Size:        114     690
 
Bpu10I CC'TnA_GC

Cuts at:      0      46     525     804 
   Size:         46     479     279

  Fragments arranged by size: 

                 479     279      46
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     772     804 
   Size:        772      32
 
BsaHI Gr'CG_yC

Cuts at:      0     255     804 
   Size:        255     549
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0       7      10      13     168     171     416     804 
   Size:          7       3       3     155       3     245     388

  Fragments arranged by size: 

                 388     245     155       7       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     623     804 
   Size:        623     181
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     624     734     804 
   Size:        624     110      70

  Fragments arranged by size: 

                 624     110      70
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      79     804 
   Size:         79     725
 
Bsp1286I G_dGCh'C

Cuts at:      0      60     247     804 
   Size:         60     187     557

  Fragments arranged by size: 

                 557     187      60
 
BsrFI r'CCGG_y

Cuts at:      0     262     804 
   Size:        262     542
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     214     804 
   Size:        214     590
 
BstYI r'GATC_y

Cuts at:      0     305     331     804 
   Size:        305      26     473

  Fragments arranged by size: 

                 473     305      26
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     442     804 
   Size:        442     362
 
EarI CTCTTCn'nnn_

Cuts at:      0      31     174     804 
   Size:         31     143     630

  Fragments arranged by size: 

                 630     143      31
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      15     114     804 
   Size:         15      99     690

  Fragments arranged by size: 

                 690      99      15
 
HaeII r_GCGC'y

Cuts at:      0      53     158     258     804 
   Size:         53     105     100     546

  Fragments arranged by size: 

                 546     105     100      53
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     272     304     804 
   Size:        272      32     500

  Fragments arranged by size: 

                 500     272      32
 
KasI G'GCGC_C

Cuts at:      0     254     804 
   Size:        254     550
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     644     804 
   Size:        644     160
 
MslI CAynn'nnrTG

Cuts at:      0     455     804 
   Size:        455     349
 
MspA1I CmG'CkG

Cuts at:      0     103     804 
   Size:        103     701
 
NarI GG'CG_CC

Cuts at:      0     255     804 
   Size:        255     549
 
PstI C_TGCA'G

Cuts at:      0     280     305     804 
   Size:        280      25     499

  Fragments arranged by size: 

                 499     280      25
 
RsrII CG'GwC_CG

Cuts at:      0     260     804 
   Size:        260     544
 
SfcI C'TryA_G

Cuts at:      0     276     301     804 
   Size:        276      25     503

  Fragments arranged by size: 

                 503     276      25
 
SfoI GGC'GCC

Cuts at:      0     256     804 
   Size:        256     548
 
SmaI CCC'GGG

Cuts at:      0     613     804 
   Size:        613     191
 
SmlI C'TyrA_G

Cuts at:      0     787     804 
   Size:        787      17
 
SspI AAT'ATT

Cuts at:      0     368     804 
   Size:        368     436
 
StyI C'CwwG_G

Cuts at:      0     207     804 
   Size:        207     597
 
XmaI C'CCGG_G

Cuts at:      0     611     804 
   Size:        611     193
 

 Enzymes that do cut:

     AcuI     AfeI    AlwNI     ApoI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BglI    BglII     BpmI   Bpu10I    BpuEI
    BsaHI    BseRI    BseYI     BsgI    BsmBI Bsp1286I    BsrFI    BstXI
    BstYI    BtgZI     EarI  Eco57MI    HaeII    Hin4I     KasI     MmeI
     MslI   MspA1I     NarI     PstI    RsrII     SfcI     SfoI     SmaI
     SmlI     SspI     StyI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI     BcgI    BciVI     BclI    BfrBI     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BsaI    BsaAI    BsaBI
    BsaWI    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MscI     NaeI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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