Restriction map for pF1KF0007
##################################
# NO CUT ENZYMES (  100 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AgeI        AhdI        Alw44I      ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BbeI        
BcgI        BciVI       BfrI        BglII       BlnI        
BsaBI       BsaI        BseAI       BsiEI       BsiWI       
BspDI       BspEI       BspHI       BspLU11I    BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     ClaI        CpoI        DraI        DraIII      
EagI        Eam1105I    EclXI       Eco52I      EcoO65I     
EcoRI       EcoT22I     FseI        FspI        HincII      
HindII      HpaI        KasI        KpnI        MamI        
MfeI        MluI        MroI        MslI        MunI        
NarI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SalI        SbfI        
SexAI       SfoI        SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    SspI        StuI        SwaI        
TfiI        Tth111I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccII         805  CGCG                 CG^CG
AcsI         1771  GAATTT               [GA]^AATT[CT]
AcyI          306  GGCGTC               G[GA]^CG[CT]C
AocI         1702  CCTGAGG              CC^T[ACGT]AGG
ApoI         1771  GAATTT               [GA]^AATT[CT]
AvaI         1730  CCCGGG               C^[CT]CG[GA]G
BamHI        2043  GGATCC               G^GATCC
BbrPI        1420  CACGTG               CAC^GTG
BbsI         1792  GTCTTC               GAAGAC(2/6)
BclI          918  TGATCA               T^GATCA
BfaI          328  CTAG                 C^TAG
BpuAI        1792  GTCTTC               GAAGAC(2/6)
BsaAI        1420  CACGTG               [CT]AC^GT[GA]
BsaHI         306  GGCGTC               G[GA]^CG[CT]C
BsmBI        1351  GAGACG               CGTCTC(1/5)
BsmI         1642  GCATTC               GAATGC(1/-1)
BsoBI        1730  CCCGGG               C^[CT]CG[GA]G
Bsp1407I     1661  TGTACA               T^GTACA
BsrDI         623  GCAATG               GCAATG(2/0)
BsrGI        1661  TGTACA               T^GTACA
BssSI        1395  CACGAG               CACGAG(-5/-1)
BstUI         805  CGCG                 CG^CG
BstXI        1026  CCAGAACCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1702  CCTGAGG              CC^T[ACGT]AGG
DrdI         1510  GACGAATGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1702  CCTGAGG              CC^T[ACGT]AGG
EcoRV        1565  GATATC               GAT^ATC
FbaI          918  TGATCA               T^GATCA
Hin1I         306  GGCGTC               G[GA]^CG[CT]C
HindIII        43  AAGCTT               A^AGCTT
KspI          804  CCGCGG               CCGC^GG
MaeI          328  CTAG                 C^TAG
MseI          638  TTAA                 T^TAA
MvnI          805  CGCG                 CG^CG
NspI          795  GCATGC               [GA]CATG^[CT]
PmaCI        1420  CACGTG               CAC^GTG
PmlI         1420  CACGTG               CAC^GTG
SacI         1360  GAGCTC               GAGCT^C
SacII         804  CCGCGG               CCGC^GG
SapI         1813  GAAGAGC              GCTCTTC(1/4)
ScaI          596  AGTACT               AGT^ACT
SfiI         1392  GGCCACGAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI         1730  CCCGGG               CCC^GGG
SphI          795  GCATGC               GCATG^C
SspBI        1661  TGTACA               T^GTACA
Tru9I         638  TTAA                 T^TAA
Tsp509I      1772  AATT                 ^AATT
XmaCI        1730  CCCGGG               C^CCGGG
XmaI         1730  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AflIII        465  ACGTGT               A^C[GA][CT]GT
             1419  ACACGT              
Aor51HI       989  AGCGCT               AGC^GCT
             1211  AGCGCT              
BalI         1391  TGGCCA               TGG^CCA
             2049  TGGCCA              
BsaWI         209  ACCGGA               [AT]^CCGG[AT]
             1672  ACCGGA              
BsmAI        1351  GAGAC                GTCTC(1/5)
              554  GTCTC               
BsrFI          27  GCCGGC               [GA]^CCGG[CT]
             2028  GCCGGC              
Cfr10I         27  GCCGGC               [GA]^CCGG[CT]
             2028  GCCGGC              
EaeI         1391  TGGCCA               [CT]^GGCC[GA]
             2049  TGGCCA              
EarI          195  GAAGAG               CTCTTC(1/4)
             1813  GAAGAG              
Eco47III      989  AGCGCT               AGC^GCT
             1211  AGCGCT              
EcoNI        1182  CCTGCTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1647  CCTGGCACAGG         
HgaI          307  GCGTC                GACGC(5/10)
             2010  GCGTC               
HinfI         299  GACTC                G^A[ACGT]TC
             1997  GACTC               
Ksp632I       195  GAAGAG               CTCTTC(1/4)
             1813  GAAGAG              
MaeII         465  ACGT                 A^CGT
             1421  ACGT                
MluNI        1391  TGGCCA               TGG^CCA
             2049  TGGCCA              
MscI         1391  TGGCCA               TGG^CCA
             2049  TGGCCA              
NaeI           27  GCCGGC               GCC^GGC
             2028  GCCGGC              
NcoI          528  CCATGG               C^CATGG
             1032  CCATGG              
NgoMIV         27  GCCGGC               G^CCGGC
             2028  GCCGGC              
PflMI         171  CCAGAAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1383  CCAAGCCCTGG         
PleI          299  GACTC                GAGTC(4/5)
             1997  GACTC               
PpuMI         737  GGGACCC              [GA]G^G[AT]CC[CT]
             1295  GGGACCT             
PstI          412  CTGCAG               CTGCA^G
             1186  CTGCAG              
PvuII          61  CAGCTG               CAG^CTG
              607  CAGCTG              
SmlI          700  CTCAAG               C^T[CT][GA]AG
              732  CTTGAG              
TaqI          665  TCGA                 T^CGA
             1995  TCGA                
TthHB8I       665  TCGA                 T^CGA
             1995  TCGA                
Van91I        171  CCAGAAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1383  CCAAGCCCTGG         
XcmI          171  CCAGAAACTGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1637  CCACAGCATTCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
ApaI        AspHI       AvaII       BanI        BanII       
BbvCI       BbvI        BcnI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmFI       
Bsp1286I    BspMI       BsrBI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       CelII       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HpaII       HphI        ItaI        
MaeIII      MboI        MboII       MflI        MnlI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TseI        Tsp45I      TspRI       XhoII       
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