Restriction map for pF1KF0007
 (Linear) (Six-base) MAPSORT of: FLJ00007.seq  Check: 4506  from: 1  to: 2070



FLJ00007.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     351    1908    2070 
   Size:        351    1557     162

  Fragments arranged by size: 

                1557     351     162
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     214     844     868    1064    1240    2070 
   Size:         58     156     630      24     196     176     830

  Fragments arranged by size: 

                 830     630     196     176     156      58      24
 
AfeI AGC'GCT

Cuts at:      0     991    1213    2070 
   Size:        991     222     857

  Fragments arranged by size: 

                 991     857     222
 
AflIII A'CryG_T

Cuts at:      0     465    1419    2070 
   Size:        465     954     651

  Fragments arranged by size: 

                 954     651     465
 
AlwNI CAG_nnn'CTG

Cuts at:      0     177     564     609     916    1659    1866    2070 
   Size:        177     387      45     307     743     207     204

  Fragments arranged by size: 

                 743     387     307     207     204     177      45
 
ApaI G_GGCC'C

Cuts at:      0    1404    1737    1928    2070 
   Size:       1404     333     191     142

  Fragments arranged by size: 

                1404     333     191     142
 
ApoI r'AATT_y

Cuts at:      0    1771    2070 
   Size:       1771     299
 
AvaI C'yCGr_G

Cuts at:      0    1730    2070 
   Size:       1730     340
 
BamHI G'GATC_C

Cuts at:      0    2043    2070 
   Size:       2043      27
 
BanI G'GyrC_C

Cuts at:      0     206     250    1989    2070 
   Size:        206      44    1739      81

  Fragments arranged by size: 

                1739     206      81      44
 
BanII G_rGCy'C

Cuts at:      0    1364    1404    1637    1737    1928    2070 
   Size:       1364      40     233     100     191     142

  Fragments arranged by size: 

                1364     233     191     142     100      40
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1785    2070 
   Size:       1785     285
 
BbvCI CC'TCA_GC

Cuts at:      0     404     893    1313    2070 
   Size:        404     489     420     757

  Fragments arranged by size: 

                 757     489     420     404
 
BclI T'GATC_A

Cuts at:      0     918    2070 
   Size:        918    1152
 
BglI GCCn_nnn'nGGC

Cuts at:      0     280    1177    1399    2070 
   Size:        280     897     222     671

  Fragments arranged by size: 

                 897     671     280     222
 
BlpI GC'TnA_GC

Cuts at:      0      64     430    1322    1693    2070 
   Size:         64     366     892     371     377

  Fragments arranged by size: 

                 892     377     371     366      64
 
Bme1580I G_kGCm'C

Cuts at:      0     209    1146    1404    1713    1737    1928    2070 
   Size:        209     937     258     309      24     191     142

  Fragments arranged by size: 

                 937     309     258     209     191     142      24
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     579     588    2070 
   Size:        579       9    1482

  Fragments arranged by size: 

                1482     579       9
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     154     286     373     541     811     955     994 
   Size:        127      27     132      87     168     270     144      39

Cuts at:    994    1078    1504    1782    2070 
   Size:         84     426     278     288

  Fragments arranged by size: 

                 426     288     278     270     168     144     132     127
                  87      84      39      27
 
Bpu10I CC'TnA_GC

Cuts at:      0     367     404     754     893    1313    1559    2070 
   Size:        367      37     350     139     420     246     511

  Fragments arranged by size: 

                 511     420     367     350     246     139      37
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     685     753    2070 
   Size:        685      68    1317

  Fragments arranged by size: 

                1317     685      68
 
BsaAI yAC'GTr

Cuts at:      0    1422    2070 
   Size:       1422     648
 
BsaHI Gr'CG_yC

Cuts at:      0     307    2070 
   Size:        307    1763
 
BsaWI w'CCGG_w

Cuts at:      0     209    1672    2070 
   Size:        209    1463     398

  Fragments arranged by size: 

                1463     398     209
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1462    1492    2070 
   Size:       1462      30     578

  Fragments arranged by size: 

                1462     578      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1363    1384    1510    1702    1871    2070 
   Size:       1363      21     126     192     169     199

  Fragments arranged by size: 

                1363     199     192     169     126      21
 
BseYI C'CCAG_C

Cuts at:      0     283     602     833    1885    2070 
   Size:        283     319     231    1052     185

  Fragments arranged by size: 

                1052     319     283     231     185
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1400    1703    1886    1916    2070 
   Size:       1400     303     183      30     154

  Fragments arranged by size: 

                1400     303     183     154      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     571     731    1364    2070 
   Size:        571     160     633     706

  Fragments arranged by size: 

                 706     633     571     160
 
BsmI GAATG_Cn'

Cuts at:      0    1642    2070 
   Size:       1642     428
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1345    2070 
   Size:       1345     725
 
Bsp1286I G_dGCh'C

Cuts at:      0     209     571     731    1146    1364    1404    1637    1713 
   Size:        209     362     160     415     218      40     233      76

Cuts at:   1713    1737    1928    2070 
   Size:         24     191     142

  Fragments arranged by size: 

                 415     362     233     218     209     191     160     142
                  76      40      24
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     351     641    1908    2070 
   Size:        351     290    1267     162

  Fragments arranged by size: 

                1267     351     290     162
 
BsrBI CCG'CTC

Cuts at:      0     215    1086    1263    2070 
   Size:        215     871     177     807

  Fragments arranged by size: 

                 871     807     215     177
 
BsrDI GCAATG_nn'

Cuts at:      0     630    2070 
   Size:        630    1440
 
BsrFI r'CCGG_y

Cuts at:      0      27    2028    2070 
   Size:         27    2001      42

  Fragments arranged by size: 

                2001      42      27
 
BsrGI T'GTAC_A

Cuts at:      0    1661    2070 
   Size:       1661     409
 
BssSI C'ACGA_G

Cuts at:      0    1395    2070 
   Size:       1395     675
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1033    2070 
   Size:       1033    1037
 
BstYI r'GATC_y

Cuts at:      0      99     118     533    1022    2043    2058    2070 
   Size:         99      19     415     489    1021      15      12

  Fragments arranged by size: 

                1021     489     415      99      19      15      12
 
Bsu36I CC'TnA_GG

Cuts at:      0    1703    2070 
   Size:       1703     367
 
BtgI C'CryG_G

Cuts at:      0     278     528     804    1032    2070 
   Size:        278     250     276     228    1038

  Fragments arranged by size: 

                1038     278     276     250     228
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1516    2070 
   Size:       1516     554
 
EaeI y'GGCC_r

Cuts at:      0    1391    2049    2070 
   Size:       1391     658      21

  Fragments arranged by size: 

                1391     658      21
 
EarI CTCTTCn'nnn_

Cuts at:      0     190    1808    2070 
   Size:        190    1618     262

  Fragments arranged by size: 

                1618     262     190
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     112     360     570    1126    2070 
   Size:        112     248     210     556     944

  Fragments arranged by size: 

                 944     556     248     210     112
 
EcoICRI GAG'CTC

Cuts at:      0    1362    2070 
   Size:       1362     708
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     127     154     214     286     373     541     811 
   Size:         58      69      27      60      72      87     168     270

Cuts at:    811     844     868     955     994    1064    1078    1240    1504 
   Size:         33      24      87      39      70      14     162     264

Cuts at:   1504    1782    2070 
   Size:        278     288

  Fragments arranged by size: 

                 288     278     270     264     168     162      87      87
                  72      70      69      60      58      39      33      27
                  24      14
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1186    1651    2070 
   Size:       1186     465     419

  Fragments arranged by size: 

                1186     465     419
 
EcoO109I rG'GnC_Cy

Cuts at:      0     705     738    1296    1400    1401    1656    1925    2070 
   Size:        705      33     558     104       1     255     269     145

  Fragments arranged by size: 

                 705     558     269     255     145     104      33       1
            
 
EcoRV GAT'ATC

Cuts at:      0    1567    2070 
   Size:       1567     503
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      66    1837    2070 
   Size:         66    1771     233

  Fragments arranged by size: 

                1771     233      66
 
HaeII r_GCGC'y

Cuts at:      0     261     353     993    1215    2070 
   Size:        261      92     640     222     855

  Fragments arranged by size: 

                 855     640     261     222      92
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     103     135     423     455    1118    1150    1977    2009 
   Size:        103      32     288      32     663      32     827      32

Cuts at:   2009    2070 
   Size:         61

  Fragments arranged by size: 

                 827     663     288     103      61      32      32      32
                  32
 
HindIII A'AGCT_T

Cuts at:      0      43    2070 
   Size:         43    2027
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     421    1330    1898    2070 
   Size:        421     909     568     172

  Fragments arranged by size: 

                 909     568     421     172
 
MscI TGG'CCA

Cuts at:      0    1393    2051    2070 
   Size:       1393     658      19

  Fragments arranged by size: 

                1393     658      19
 
MspA1I CmG'CkG

Cuts at:      0      63     459     609     806    1312    2070 
   Size:         63     396     150     197     506     758

  Fragments arranged by size: 

                 758     506     396     197     150      63
 
NaeI GCC'GGC

Cuts at:      0      29    2030    2070 
   Size:         29    2001      40

  Fragments arranged by size: 

                2001      40      29
 
NcoI C'CATG_G

Cuts at:      0     528    1032    2070 
   Size:        528     504    1038

  Fragments arranged by size: 

                1038     528     504
 
NgoMIV G'CCGG_C

Cuts at:      0      27    2028    2070 
   Size:         27    2001      42

  Fragments arranged by size: 

                2001      42      27
 
NspI r_CATG'y

Cuts at:      0     799    2070 
   Size:        799    1271
 
PasI CC'CwG_GG

Cuts at:      0    1831    1969    2070 
   Size:       1831     138     101

  Fragments arranged by size: 

                1831     138     101
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     177    1389    2070 
   Size:        177    1212     681

  Fragments arranged by size: 

                1212     681     177
 
PfoI T'CCnGG_A

Cuts at:      0      53     422     788     932    1577    1976    2070 
   Size:         53     369     366     144     645     399      94

  Fragments arranged by size: 

                 645     399     369     366     144      94      53
 
PmlI CAC'GTG

Cuts at:      0    1422    2070 
   Size:       1422     648
 
PpuMI rG'GwC_Cy

Cuts at:      0     738    1296    2070 
   Size:        738     558     774

  Fragments arranged by size: 

                 774     738     558
 
PspOMI G'GGCC_C

Cuts at:      0    1400    1733    1924    2070 
   Size:       1400     333     191     146

  Fragments arranged by size: 

                1400     333     191     146
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1097    1129    2070 
   Size:       1097      32     941

  Fragments arranged by size: 

                1097     941      32
 
PstI C_TGCA'G

Cuts at:      0     416    1190    2070 
   Size:        416     774     880

  Fragments arranged by size: 

                 880     774     416
 
PvuII CAG'CTG

Cuts at:      0      63     609    2070 
   Size:         63     546    1461

  Fragments arranged by size: 

                1461     546      63
 
SacI G_AGCT'C

Cuts at:      0    1364    2070 
   Size:       1364     706
 
SacII CC_GC'GG

Cuts at:      0     807    2070 
   Size:        807    1263
 
SanDI GG'GwC_CC

Cuts at:      0     738    2070 
   Size:        738    1332
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1808    2070 
   Size:       1808     262
 
ScaI AGT'ACT

Cuts at:      0     598    2070 
   Size:        598    1472
 
SfcI C'TryA_G

Cuts at:      0     123     412    1186    2070 
   Size:        123     289     774     884

  Fragments arranged by size: 

                 884     774     289     123
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1399    2070 
   Size:       1399     671
 
SmaI CCC'GGG

Cuts at:      0    1732    2070 
   Size:       1732     338
 
SmlI C'TyrA_G

Cuts at:      0     700     732    2070 
   Size:        700      32    1338

  Fragments arranged by size: 

                1338     700      32
 
SphI G_CATG'C

Cuts at:      0     799    2070 
   Size:        799    1271
 
StyI C'CwwG_G

Cuts at:      0     353     476     528    1032    2070 
   Size:        353     123      52     504    1038

  Fragments arranged by size: 

                1038     504     353     123      52
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     329    2069    2070 
   Size:        329    1740       1

  Fragments arranged by size: 

                1740     329       1
 
TatI w'GTAC_w

Cuts at:      0     596    1661    2070 
   Size:        596    1065     409

  Fragments arranged by size: 

                1065     596     409
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     454     486    1462    1494    2070 
   Size:        454      32     976      32     576

  Fragments arranged by size: 

                 976     576     454      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     178    1644    2070 
   Size:        178    1466     426

  Fragments arranged by size: 

                1466     426     178
 
XmaI C'CCGG_G

Cuts at:      0    1730    2070 
   Size:       1730     340
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII    AlwNI     ApaI     ApoI     AvaI
    BamHI     BanI    BanII     BbsI    BbvCI     BclI     BglI     BlpI
 Bme1580I     BmrI     BpmI   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI    BstXI    BstYI
   Bsu36I     BtgI     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRV     FalI    HaeII    Hin4I  HindIII     MmeI
     MscI   MspA1I     NaeI     NcoI   NgoMIV     NspI     PasI    PflMI
     PfoI     PmlI    PpuMI   PspOMI     PsrI     PstI    PvuII     SacI
    SacII    SanDI     SapI     ScaI     SfcI     SfiI     SmaI     SmlI
     SphI     StyI    TaqII     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
     BbeI     BcgI    BciVI    BfrBI    BglII    BmgBI     BmtI     BplI
     BsaI    BsaBI    BsiEI    BsiWI    BspEI    BspHI   BssHII   BstAPI
    BstBI   BstEII  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     EagI    EcoRI     FseI     FspI    FspAI   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MslI     NarI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PciI     PmeI     PpiI    PshAI
     PsiI    PspXI     PvuI    RsrII     SalI     SbfI    SexAI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI
  Tth111I     XbaI     XhoI     XmnI     ZraI
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