Restriction map for pF1KB6517
 (Linear) (Six-base) MAPSORT of: KIBB6517.seq  Check: 6221  from: 1  to: 1086



KIBB6517.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AccI GT'mk_AC

Cuts at:      0      60    1086 
   Size:         60    1026
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1044    1086 
   Size:       1044      42
 
AhdI GACnn_n'nnGTC

Cuts at:      0     806    1086 
   Size:        806     280
 
ApaLI G'TGCA_C

Cuts at:      0      14    1086 
   Size:         14    1072
 
ApoI r'AATT_y

Cuts at:      0     747    1007    1050    1086 
   Size:        747     260      43      36

  Fragments arranged by size: 

                 747     260      43      36
 
BamHI G'GATC_C

Cuts at:      0     586    1086 
   Size:        586     500
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      31     202     607    1086 
   Size:         31     171     405     479

  Fragments arranged by size: 

                 479     405     171      31
 
BglII A'GATC_T

Cuts at:      0     592     969    1086 
   Size:        592     377     117

  Fragments arranged by size: 

                 592     377     117
 
Bme1580I G_kGCm'C

Cuts at:      0      18     332    1086 
   Size:         18     314     754

  Fragments arranged by size: 

                 754     314      18
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     257     812    1086 
   Size:        257     555     274

  Fragments arranged by size: 

                 555     274     257
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     786    1009    1086 
   Size:        786     223      77

  Fragments arranged by size: 

                 786     223      77
 
BsaBI GATnn'nnATC

Cuts at:      0     591    1086 
   Size:        591     495
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     157     187    1086 
   Size:        157      30     899

  Fragments arranged by size: 

                 899     157      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     282     420     480    1086 
   Size:        282     138      60     606

  Fragments arranged by size: 

                 606     282     138      60
 
BsiHKAI G_wGCw'C

Cuts at:      0      18    1086 
   Size:         18    1068
 
BsmI GAATG_Cn'

Cuts at:      0     553    1086 
   Size:        553     533
 
Bsp1286I G_dGCh'C

Cuts at:      0      18     332    1086 
   Size:         18     314     754

  Fragments arranged by size: 

                 754     314      18
 
BspHI T'CATG_A

Cuts at:      0     240     708     797    1086 
   Size:        240     468      89     289

  Fragments arranged by size: 

                 468     289     240      89
 
BstBI TT'CG_AA

Cuts at:      0     828    1086 
   Size:        828     258
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     183    1086 
   Size:        183     903
 
BstYI r'GATC_y

Cuts at:      0     586     592     969    1086 
   Size:        586       6     377     117

  Fragments arranged by size: 

                 586     377     117       6
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      89     124    1086 
   Size:         89      35     962

  Fragments arranged by size: 

                 962      89      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     572    1086 
   Size:        572     514
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     301    1086 
   Size:        301     785
 
EaeI y'GGCC_r

Cuts at:      0     246    1086 
   Size:        246     840
 
EarI CTCTTCn'nnn_

Cuts at:      0     646    1086 
   Size:        646     440
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     257     812    1044    1086 
   Size:        257     555     232      42

  Fragments arranged by size: 

                 555     257     232      42
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1003    1086 
   Size:       1003      83
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     157     189     571     603     792     824     954     986 
   Size:        157      32     382      32     189      32     130      32

Cuts at:    986    1086 
   Size:        100

  Fragments arranged by size: 

                 382     189     157     130     100      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     762    1086 
   Size:        762     324
 
HindIII A'AGCT_T

Cuts at:      0    1077    1086 
   Size:       1077       9
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     742    1086 
   Size:        742     344
 
MscI TGG'CCA

Cuts at:      0     248    1086 
   Size:        248     838
 
MslI CAynn'nnrTG

Cuts at:      0     242    1086 
   Size:        242     844
 
NspI r_CATG'y

Cuts at:      0     677    1086 
   Size:        677     409
 
PasI CC'CwG_GG

Cuts at:      0      33    1086 
   Size:         33    1053
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1080    1086 
   Size:       1080       6
 
SfcI C'TryA_G

Cuts at:      0     206    1086 
   Size:        206     880
 
SmlI C'TyrA_G

Cuts at:      0     765    1024    1086 
   Size:        765     259      62

  Fragments arranged by size: 

                 765     259      62
 
SpeI A'CTAG_T

Cuts at:      0    1036    1086 
   Size:       1036      50
 
StuI AGG'CCT

Cuts at:      0     233    1086 
   Size:        233     853
 
StyI C'CwwG_G

Cuts at:      0     176     699    1086 
   Size:        176     523     387

  Fragments arranged by size: 

                 523     387     176
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     636    1086 
   Size:        636     450
 
TatI w'GTAC_w

Cuts at:      0     350     663     991    1086 
   Size:        350     313     328      95

  Fragments arranged by size: 

                 350     328     313      95
 
Tth111I GACn'n_nGTC

Cuts at:      0     877    1086 
   Size:        877     209
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     597     641    1086 
   Size:        597      44     445

  Fragments arranged by size: 

                 597     445      44
 

 Enzymes that do cut:

     AccI     AcuI     AhdI    ApaLI     ApoI    BamHI     BbsI    BglII
 Bme1580I     BpmI    BpuEI    BsaBI    BsaXI     BsgI  BsiHKAI     BsmI
 Bsp1286I    BspHI    BstBI    BstXI    BstYI    CspCI   DraIII     DrdI
     EaeI     EarI  Eco57MI     FalI    Hin4I   HincII  HindIII     MmeI
     MscI     MslI     NspI     PasI    PflMI     SfcI     SmlI     SpeI
     StuI     StyI    TaqII     TatI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI    AlwNI     ApaI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI     BanI    BanII     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI
     BplI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI    BseRI    BseYI
    BsiEI    BsiWI    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI   BstEII  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI     DraI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII     HpaI
     KasI     KpnI     MfeI     MluI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI     SspI     SwaI
     TstI     XbaI     XhoI     XmaI     XmnI     ZraI
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