Restriction map for pF1KB6310
 (Linear) (Six-base) MAPSORT of: KIBB6310.seq  Check: 722  from: 1  to: 840



KIBB6310.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     565     607     840 
   Size:        565      42     233

  Fragments arranged by size: 

                 565     233      42
 
AfeI AGC'GCT

Cuts at:      0     492     840 
   Size:        492     348
 
AhdI GACnn_n'nnGTC

Cuts at:      0     213     840 
   Size:        213     627
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     814     840 
   Size:        814      26
 
ApaI G_GGCC'C

Cuts at:      0     750     840 
   Size:        750      90
 
AvaI C'yCGr_G

Cuts at:      0      93     317     526     742     757     777     840 
   Size:         93     224     209     216      15      20      63

  Fragments arranged by size: 

                 224     216     209      93      63      20      15
 
BamHI G'GATC_C

Cuts at:      0     162     840 
   Size:        162     678
 
BanII G_rGCy'C

Cuts at:      0       7     680     750     784     840 
   Size:          7     673      70      34      56

  Fragments arranged by size: 

                 673      70      56      34       7
 
BbvCI CC'TCA_GC

Cuts at:      0     392     840 
   Size:        392     448
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     448     482     840 
   Size:        448      34     358

  Fragments arranged by size: 

                 448     358      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     367     542     840 
   Size:        367     175     298

  Fragments arranged by size: 

                 367     298     175
 
BglII A'GATC_T

Cuts at:      0      84     561     840 
   Size:         84     477     279

  Fragments arranged by size: 

                 477     279      84
 
Bme1580I G_kGCm'C

Cuts at:      0      39     363     750     840 
   Size:         39     324     387      90

  Fragments arranged by size: 

                 387     324      90      39
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     590     840 
   Size:        590     250
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     101     195     375     586     840 
   Size:        101      94     180     211     254

  Fragments arranged by size: 

                 254     211     180     101      94
 
Bpu10I CC'TnA_GC

Cuts at:      0     392     840 
   Size:        392     448
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      14     840 
   Size:         14     826
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      99     129     790     820     840 
   Size:         99      30     661      30      20

  Fragments arranged by size: 

                 661      99      30      30      20
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     529     624     688     832     840 
   Size:        529      95      64     144       8

  Fragments arranged by size: 

                 529     144      95      64       8
 
BseYI C'CCAG_C

Cuts at:      0     362     374     387     640     682     709     840 
   Size:        362      12      13     253      42      27     131

  Fragments arranged by size: 

                 362     253     131      42      27      13      12
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     505     840 
   Size:        295     210     335

  Fragments arranged by size: 

                 335     295     210
 
Bsp1286I G_dGCh'C

Cuts at:      0       7      39     363     680     750     784     840 
   Size:          7      32     324     317      70      34      56

  Fragments arranged by size: 

                 324     317      70      56      34      32       7
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     312     840 
   Size:        312     528
 
BstYI r'GATC_y

Cuts at:      0      84     162     561     840 
   Size:         84      78     399     279

  Fragments arranged by size: 

                 399     279      84      78
 
Bsu36I CC'TnA_GG

Cuts at:      0     671     840 
   Size:        671     169
 
BtgI C'CryG_G

Cuts at:      0     110     305     311     794     840 
   Size:        110     195       6     483      46

  Fragments arranged by size: 

                 483     195     110      46       6
 
EaeI y'GGCC_r

Cuts at:      0     248     308     840 
   Size:        248      60     532

  Fragments arranged by size: 

                 532     248      60
 
EarI CTCTTCn'nnn_

Cuts at:      0     506     840 
   Size:        506     334
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     101     195     375     565     586     607     840 
   Size:        101      94     180     190      21      21     233

  Fragments arranged by size: 

                 233     190     180     101      94      21      21
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     814     840 
   Size:        814      26
 
EcoO109I rG'GnC_Cy

Cuts at:      0     215     746     754     767     840 
   Size:        215     531       8      13      73

  Fragments arranged by size: 

                 531     215      73      13       8
 
HaeII r_GCGC'y

Cuts at:      0     494     840 
   Size:        494     346
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      76     108     199     231     790     822     840 
   Size:         76      32      91      32     559      32      18

  Fragments arranged by size: 

                 559      91      76      32      32      32      18
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     230     840 
   Size:        230     610
 
MscI TGG'CCA

Cuts at:      0     250     840 
   Size:        250     590
 
MslI CAynn'nnrTG

Cuts at:      0     310     840 
   Size:        310     530
 
MspA1I CmG'CkG

Cuts at:      0     366     391     659     691     803     840 
   Size:        366      25     268      32     112      37

  Fragments arranged by size: 

                 366     268     112      37      32      25
 
NcoI C'CATG_G

Cuts at:      0     311     794     840 
   Size:        311     483      46

  Fragments arranged by size: 

                 483     311      46
 
NruI TCG'CGA

Cuts at:      0      18     840 
   Size:         18     822
 
PasI CC'CwG_GG

Cuts at:      0      60     540     840 
   Size:         60     480     300

  Fragments arranged by size: 

                 480     300      60
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     564     840 
   Size:        564     276
 
PfoI T'CCnGG_A

Cuts at:      0     416     840 
   Size:        416     424
 
PpuMI rG'GwC_Cy

Cuts at:      0     215     754     767     840 
   Size:        215     539      13      73

  Fragments arranged by size: 

                 539     215      73      13
 
PshAI GACnn'nnGTC

Cuts at:      0     659     840 
   Size:        659     181
 
PspOMI G'GGCC_C

Cuts at:      0     746     840 
   Size:        746      94
 
PstI C_TGCA'G

Cuts at:      0     450     725     840 
   Size:        450     275     115

  Fragments arranged by size: 

                 450     275     115
 
PvuII CAG'CTG

Cuts at:      0     391     659     840 
   Size:        391     268     181

  Fragments arranged by size: 

                 391     268     181
 
SanDI GG'GwC_CC

Cuts at:      0     767     840 
   Size:        767      73
 
SfcI C'TryA_G

Cuts at:      0     446     721     840 
   Size:        446     275     119

  Fragments arranged by size: 

                 446     275     119
 
SmaI CCC'GGG

Cuts at:      0     744     779     840 
   Size:        744      35      61

  Fragments arranged by size: 

                 744      61      35
 
StuI AGG'CCT

Cuts at:      0     628     840 
   Size:        628     212
 
StyI C'CwwG_G

Cuts at:      0     166     311     750     794     840 
   Size:        166     145     439      44      46

  Fragments arranged by size: 

                 439     166     145      46      44
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      99     131     840 
   Size:         99      32     709

  Fragments arranged by size: 

                 709      99      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     124     840 
   Size:        124     716
 
XmaI C'CCGG_G

Cuts at:      0     742     777     840 
   Size:        742      35      63

  Fragments arranged by size: 

                 742      63      35
 

 Enzymes that do cut:

     AcuI     AfeI     AhdI     AloI     ApaI     AvaI    BamHI    BanII
    BbvCI     BcgI     BglI    BglII Bme1580I     BmrI     BpmI   Bpu10I
     BsaI    BsaXI    BseRI    BseYI     BsgI Bsp1286I    BstXI    BstYI
   Bsu36I     BtgI     EaeI     EarI  Eco57MI    EcoNI EcoO109I    HaeII
    Hin4I     MmeI     MscI     MslI   MspA1I     NcoI     NruI     PasI
    PflMI     PfoI    PpuMI    PshAI   PspOMI     PstI    PvuII    SanDI
     SfcI     SmaI     StuI     StyI     TstI  Tth111I     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AleI     AlfI    AlwNI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI     BanI     BbeI     BbsI    BciVI     BclI    BfrBI
     BlpI    BmgBI     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaHI
    BsaWI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NsiI     NspI     PacI     PciI     PmeI     PmlI     PpiI     PsiI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII     TatI
     XbaI     XcmI     XhoI     XmnI     ZraI
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