Restriction map for pF1KE0487
##################################
# NO CUT ENZYMES (  122 enzymes) #
##################################

Acc65I      AccI        AclI        AcsI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanII       BbrPI       BcgI        BfaI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BsaAI       BseRI       BsiEI       BsiWI       BsmI        
BsoBI       Bsp1407I    BspDI       BspLU11I    BsrBI       
BsrDI       BsrGI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraIII      EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRV       FseI        FspI        HincII      
HindII      HindIII     HpaI        KpnI        KspI        
MaeI        MfeI        MluI        MluNI       MscI        
MunI        NdeI        NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        SwaI        
Tth111I     Van91I      XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AccII         946  CGCG                 CG^CG
AfaI          552  GTAC                 GT^AC
AspHI         772  GAGCAC               G[AT]GC[AT]^C
BanI          421  GGCGCC               G^G[CT][GA]CC
BbeI          421  GGCGCC               GGCGC^C
BbsI          802  GAAGAC               GAAGAC(2/6)
BbvCI         402  CCTCAGC              CCTCAGC(-5/-2)
BciVI         856  GTATCC               GTATCC(6/5)
BclI          717  TGATCA               T^GATCA
BglII         914  AGATCT               A^GATCT
BpuAI         802  GAAGAC               GAAGAC(2/6)
BsaBI         937  GATGCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          706  GAGACC               GGTCTC(1/5)
BsiHKAI       772  GAGCAC               G[AT]GC[AT]^C
BsmBI         215  GAGACG               CGTCTC(1/5)
BspHI         714  TCATGA               T^CATGA
BspMI         452  ACCTGC               ACCTGC(4/8)
BsrFI         470  GCCGGC               [GA]^CCGG[CT]
BsrI          106  ACTGG                ACTGG(1/-1)
BssSI         150  CTCGTG               CACGAG(-5/-1)
BstAPI        774  GCACTACATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         946  CGCG                 CG^CG
BstXI         959  CCACATTATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         914  AGATCT               [GA]^GATC[CT]
Cfr10I        470  GCCGGC               [GA]^CCGG[CT]
DraI           54  TTTAAA               TTT^AAA
DrdI          637  GACGCAGAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          468  TGGCCG               [CT]^GGCC[GA]
EarI         1006  GAAGAG               CTCTTC(1/4)
EcoT22I       974  ATGCAT               ATGCA^T
FauI          593  GCGGG                CCCGC(4/6)
FbaI          717  TGATCA               T^GATCA
HaeII         421  GGCGCC               [GA]GCGC^[CT]
HphI          295  TCACC                GGTGA(8/7)
KasI          421  GGCGCC               G^GCGCC
Ksp632I      1006  GAAGAG               CTCTTC(1/4)
MamI          937  GATGCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          914  AGATCT               [GA]^GATC[CT]
MslI          780  CATGCTTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        193  CAGCTG               C[AC]G^C[GT]G
MvnI          946  CGCG                 CG^CG
NaeI          470  GCCGGC               GCC^GGC
NarI          421  GGCGCC               GG^CGCC
NgoMIV        470  GCCGGC               G^CCGGC
NsiI          974  ATGCAT               ATGCA^T
PvuII         193  CAGCTG               CAG^CTG
RcaI          714  TCATGA               T^CATGA
RsaI          552  GTAC                 GT^AC
SfoI          421  GGCGCC               GGC^GCC
SmlI          632  CTCAAG               C^T[CT][GA]AG
SphI          976  GCATGC               GCATG^C
StuI          461  AGGCCT               AGG^CCT
Tsp509I      1017  AATT                 ^AATT
XcmI          488  CCATGGAGTCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         914  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AatII         217  GACGTC               GACGT^C
              625  GACGTC              
AlwI          282  GATCC                GGATC(4/5)
              399  GATCC               
BmyI          541  GGGCAC               G[AGT]GC[ACT]^C
              772  GAGCAC              
BsgI          205  CTGCAC               GTGCAG(16/14)
              838  CTGCAC              
BsmAI         215  GAGAC                GTCTC(1/5)
              706  GAGAC               
Bsp1286I      541  GGGCAC               G[AGT]GC[ACT]^C
              772  GAGCAC              
BstF5I         66  CATCC                GGATG(2/0)
              372  CATCC               
Cac8I         470  GCCGGC               GC[ACGT]^[ACGT]GC
              976  GCATGC              
CfoI          116  GCGC                 GCG^C
              422  GCGC                
DdeI          403  CTCAG                C^T[ACGT]AG
              911  CTCAG               
DsaI          425  CCATGG               C^C[GA][CT]GG
              488  CCATGG              
FokI           66  CATCC                GGATG(9/13)
              372  CATCC               
HhaI          116  GCGC                 GCG^C
              422  GCGC                
HinP1I        116  GCGC                 G^CGC
              422  GCGC                
MaeII         218  ACGT                 A^CGT
              626  ACGT                
MseI           55  TTAA                 T^TAA
              815  TTAA                
NcoI          425  CCATGG               C^CATGG
              488  CCATGG              
NspI          779  ACATGC               [GA]CATG^[CT]
              976  GCATGC              
PleI          378  GAGTC                GAGTC(4/5)
              493  GAGTC               
PpuMI         254  GGGACCT              [GA]G^G[AT]CC[CT]
              521  AGGTCCT             
PshAI         105  GACTGGCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
              516  GACCAAGGTC          
PstI          185  CTGCAG               CTGCA^G
              391  CTGCAG              
SfcI          185  CTGCAG               C^T[GA][CT]AG
              391  CTGCAG              
Tru9I          55  TTAA                 T^TAA
              815  TTAA                
TspRI           8  CAGTG                CA[GC]TG[ACGT][ACGT]^
              122  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AccIII      AciI        AcyI        AluI        AlwNI       
AvaII       BbvI        BcnI        BpmI        BsaHI       
BsaJI       BsaWI       BseAI       BsiYI       BslI        
BsmFI       BspEI       BssKI       BstNI       Cfr13I      
DpnII       DraII       EcoNI       EcoO109I    EcoRII      
EcoT14I     Fnu4HI      HaeIII      HapII       HgaI        
Hin1I       HinfI       HpaII       ItaI        MaeIII      
MboI        MboII       MnlI        MroI        MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       Sau3AI      Sau96I      ScrFI       SfaNI       
StyI        TaqI        TfiI        TseI        Tsp45I      
TthHB8I     
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