Restriction map for pF1KE0487
 (Linear) (Six-base) MAPSORT of: KIEE0487.seq  Check: 6140  from: 1  to: 1071



KIEE0487.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AatII G_ACGT'C

Cuts at:      0     221     629    1071 
   Size:        221     408     442

  Fragments arranged by size: 

                 442     408     221
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     811    1071 
   Size:        811     260
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     920    1071 
   Size:        920     151
 
AlwNI CAG_nnn'CTG

Cuts at:      0      87     940    1051    1071 
   Size:         87     853     111      20

  Fragments arranged by size: 

                 853     111      87      20
 
BanI G'GyrC_C

Cuts at:      0     421    1071 
   Size:        421     650
 
BbeI G_GCGC'C

Cuts at:      0     425    1071 
   Size:        425     646
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     809    1071 
   Size:        809     262
 
BbvCI CC'TCA_GC

Cuts at:      0     403    1071 
   Size:        403     668
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     867    1071 
   Size:        867     204
 
BclI T'GATC_A

Cuts at:      0     717    1071 
   Size:        717     354
 
BfrBI ATG'CAT

Cuts at:      0     976    1071 
   Size:        976      95
 
BglII A'GATC_T

Cuts at:      0     914    1071 
   Size:        914     157
 
Bme1580I G_kGCm'C

Cuts at:      0     545    1071 
   Size:        545     526
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     382     478     643    1071 
   Size:        217     165      96     165     428

  Fragments arranged by size: 

                 428     217     165     165      96
 
Bpu10I CC'TnA_GC

Cuts at:      0     403    1071 
   Size:        403     668
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     617    1071 
   Size:        617     454
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     700    1071 
   Size:        700     371
 
BsaBI GATnn'nnATC

Cuts at:      0     941    1071 
   Size:        941     130
 
BsaHI Gr'CG_yC

Cuts at:      0     110     218     422     557     626    1071 
   Size:        110     108     204     135      69     445

  Fragments arranged by size: 

                 445     204     135     110     108      69
 
BsaWI w'CCGG_w

Cuts at:      0      68     230     284     374    1071 
   Size:         68     162      54      90     697

  Fragments arranged by size: 

                 697     162      90      68      54
 
BseYI C'CCAG_C

Cuts at:      0     319    1071 
   Size:        319     752
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     823    1071 
   Size:        190     633     248

  Fragments arranged by size: 

                 633     248     190
 
BsiHKAI G_wGCw'C

Cuts at:      0     776    1071 
   Size:        776     295
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     209    1071 
   Size:        209     862
 
Bsp1286I G_dGCh'C

Cuts at:      0     545     776    1071 
   Size:        545     231     295

  Fragments arranged by size: 

                 545     295     231
 
BspEI T'CCGG_A

Cuts at:      0      68     230     284     374    1071 
   Size:         68     162      54      90     697

  Fragments arranged by size: 

                 697     162      90      68      54
 
BspHI T'CATG_A

Cuts at:      0     714    1071 
   Size:        714     357
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     461    1071 
   Size:        461     610
 
BsrFI r'CCGG_y

Cuts at:      0     470    1071 
   Size:        470     601
 
BssSI C'ACGA_G

Cuts at:      0     150    1071 
   Size:        150     921
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     780    1071 
   Size:        780     291
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     966    1071 
   Size:        966     105
 
BstYI r'GATC_y

Cuts at:      0     914    1071 
   Size:        914     157
 
BtgI C'CryG_G

Cuts at:      0     425     488    1071 
   Size:        425      63     583

  Fragments arranged by size: 

                 583     425      63
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     323    1071 
   Size:        323     748
 
BtsI GCAGTG_nn'

Cuts at:      0     128    1071 
   Size:        128     943
 
DraI TTT'AAA

Cuts at:      0      56    1071 
   Size:         56    1015
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     643    1071 
   Size:        643     428
 
EaeI y'GGCC_r

Cuts at:      0     468    1071 
   Size:        468     603
 
EarI CTCTTCn'nnn_

Cuts at:      0    1001    1071 
   Size:       1001      70
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     382     478     643     811    1071 
   Size:        217     165      96     165     168     260

  Fragments arranged by size: 

                 260     217     168     165     165      96
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     302     343     457    1071 
   Size:        302      41     114     614

  Fragments arranged by size: 

                 614     302     114      41
 
EcoO109I rG'GnC_Cy

Cuts at:      0     255     522     732    1071 
   Size:        255     267     210     339

  Fragments arranged by size: 

                 339     267     255     210
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     339    1071 
   Size:        339     732
 
HaeII r_GCGC'y

Cuts at:      0     425    1071 
   Size:        425     646
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     282     314    1071 
   Size:        282      32     757

  Fragments arranged by size: 

                 757     282      32
 
KasI G'GCGC_C

Cuts at:      0     421    1071 
   Size:        421     650
 
MslI CAynn'nnrTG

Cuts at:      0     784    1071 
   Size:        784     287
 
MspA1I CmG'CkG

Cuts at:      0     195    1071 
   Size:        195     876
 
NaeI GCC'GGC

Cuts at:      0     472    1071 
   Size:        472     599
 
NarI GG'CG_CC

Cuts at:      0     422    1071 
   Size:        422     649
 
NcoI C'CATG_G

Cuts at:      0     425     488    1071 
   Size:        425      63     583

  Fragments arranged by size: 

                 583     425      63
 
NgoMIV G'CCGG_C

Cuts at:      0     470    1071 
   Size:        470     601
 
NsiI A_TGCA'T

Cuts at:      0     978    1071 
   Size:        978      93
 
NspI r_CATG'y

Cuts at:      0     783     980    1071 
   Size:        783     197      91

  Fragments arranged by size: 

                 783     197      91
 
PfoI T'CCnGG_A

Cuts at:      0     329     885    1071 
   Size:        329     556     186

  Fragments arranged by size: 

                 556     329     186
 
PpuMI rG'GwC_Cy

Cuts at:      0     255     522    1071 
   Size:        255     267     549

  Fragments arranged by size: 

                 549     267     255
 
PshAI GACnn'nnGTC

Cuts at:      0     109     520    1071 
   Size:        109     411     551

  Fragments arranged by size: 

                 551     411     109
 
PstI C_TGCA'G

Cuts at:      0     189     395    1071 
   Size:        189     206     676

  Fragments arranged by size: 

                 676     206     189
 
PvuII CAG'CTG

Cuts at:      0     195    1071 
   Size:        195     876
 
SfcI C'TryA_G

Cuts at:      0     185     391    1071 
   Size:        185     206     680

  Fragments arranged by size: 

                 680     206     185
 
SfoI GGC'GCC

Cuts at:      0     423    1071 
   Size:        423     648
 
SmlI C'TyrA_G

Cuts at:      0     632    1071 
   Size:        632     439
 
SphI G_CATG'C

Cuts at:      0     980    1071 
   Size:        980      91
 
StuI AGG'CCT

Cuts at:      0     463    1071 
   Size:        463     608
 
StyI C'CwwG_G

Cuts at:      0     425     488     518    1071 
   Size:        425      63      30     553

  Fragments arranged by size: 

                 553     425      63      30
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     495    1071 
   Size:        495     576
 
ZraI GAC'GTC

Cuts at:      0     219     627    1071 
   Size:        219     408     444

  Fragments arranged by size: 

                 444     408     219
 

 Enzymes that do cut:

    AatII     AcuI     AlfI    AlwNI     BanI     BbeI     BbsI    BbvCI
    BciVI     BclI    BfrBI    BglII Bme1580I     BpmI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI    BseYI     BsgI  BsiHKAI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrFI    BssSI   BstAPI    BstXI
    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EarI
  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I     KasI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NsiI     NspI     PfoI
    PpuMI    PshAI     PstI    PvuII     SfcI     SfoI     SmlI     SphI
     StuI     StyI     XcmI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BcgI     BglI
     BlpI    BmgBI     BmrI     BmtI     BplI    BsaAI    BsaXI    BseRI
    BsiEI    BsiWI     BsmI    BsrBI    BsrDI    BsrGI   BssHII    BstBI
   BstEII  BstZ17I   Bsu36I     ClaI    CspCI   DraIII     EagI     EciI
  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     NdeI     NheI
     NotI     NruI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
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