Restriction map for pF1KE0507
 (Linear) (Six-base) MAPSORT of: KIEE0507.seq  Check: 5476  from: 1  to: 777



KIEE0507.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     305     777 
   Size:        305     472
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     629     777 
   Size:        629     148
 
AlwNI CAG_nnn'CTG

Cuts at:      0     423     467     777 
   Size:        423      44     310

  Fragments arranged by size: 

                 423     310      44
 
ApaI G_GGCC'C

Cuts at:      0      88     186     613     738     777 
   Size:         88      98     427     125      39

  Fragments arranged by size: 

                 427     125      98      88      39
 
AvaI C'yCGr_G

Cuts at:      0     244     350     777 
   Size:        244     106     427

  Fragments arranged by size: 

                 427     244     106
 
AvrII C'CTAG_G

Cuts at:      0     627     777 
   Size:        627     150
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     373     406     777 
   Size:        373      33     371

  Fragments arranged by size: 

                 373     371      33
 
BanII G_rGCy'C

Cuts at:      0      88     186     613     646     738     777 
   Size:         88      98     427      33      92      39

  Fragments arranged by size: 

                 427      98      92      88      39      33
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     235     269     777 
   Size:        235      34     508

  Fragments arranged by size: 

                 508     235      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     328     545     777 
   Size:        328     217     232

  Fragments arranged by size: 

                 328     232     217
 
Bme1580I G_kGCm'C

Cuts at:      0      88     186     245     613     738     777 
   Size:         88      98      59     368     125      39

  Fragments arranged by size: 

                 368     125      98      88      59      39
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     101     777 
   Size:        101     676
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     302     526     777 
   Size:        302     224     251

  Fragments arranged by size: 

                 302     251     224
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     777 
   Size:        124     653
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     652     777 
   Size:        652     125
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     385     415     508     538     777 
   Size:        385      30      93      30     239

  Fragments arranged by size: 

                 385     239      93      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      30     262     613     777 
   Size:         30     232     351     164

  Fragments arranged by size: 

                 351     232     164      30
 
BseYI C'CCAG_C

Cuts at:      0      48     777 
   Size:         48     729
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     198     777 
   Size:        198     579
 
Bsp1286I G_dGCh'C

Cuts at:      0      88     186     245     613     646     738     777 
   Size:         88      98      59     368      33      92      39

  Fragments arranged by size: 

                 368      98      92      88      59      39      33
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     158     305     560     777 
   Size:        158     147     255     217

  Fragments arranged by size: 

                 255     217     158     147
 
BsrBI CCG'CTC

Cuts at:      0     440     777 
   Size:        440     337
 
BsrFI r'CCGG_y

Cuts at:      0     315     777 
   Size:        315     462
 
BtsI GCAGTG_nn'

Cuts at:      0     516     724     777 
   Size:        516     208      53

  Fragments arranged by size: 

                 516     208      53
 
EaeI y'GGCC_r

Cuts at:      0     117     227     513     693     777 
   Size:        117     110     286     180      84

  Fragments arranged by size: 

                 286     180     117     110      84
 
EarI CTCTTCn'nnn_

Cuts at:      0     724     777 
   Size:        724      53
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     302     526     777 
   Size:        302     224     251

  Fragments arranged by size: 

                 302     251     224
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     526     631     777 
   Size:        526     105     146

  Fragments arranged by size: 

                 526     146     105
 
EcoO109I rG'GnC_Cy

Cuts at:      0      84      92     183     609     610     734     777 
   Size:         84       8      91     426       1     124      43

  Fragments arranged by size: 

                 426     124      91      84      43       8       1
 
FspI TGC'GCA

Cuts at:      0      73     777 
   Size:         73     704
 
HindIII A'AGCT_T

Cuts at:      0     357     777 
   Size:        357     420
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     371     777 
   Size:        371     406
 
MscI TGG'CCA

Cuts at:      0     515     695     777 
   Size:        515     180      82

  Fragments arranged by size: 

                 515     180      82
 
MspA1I CmG'CkG

Cuts at:      0      52     467     777 
   Size:         52     415     310

  Fragments arranged by size: 

                 415     310      52
 
NaeI GCC'GGC

Cuts at:      0     317     777 
   Size:        317     460
 
NgoMIV G'CCGG_C

Cuts at:      0     315     777 
   Size:        315     462
 
NruI TCG'CGA

Cuts at:      0     173     777 
   Size:        173     604
 
NspI r_CATG'y

Cuts at:      0     372     777 
   Size:        372     405
 
PasI CC'CwG_GG

Cuts at:      0      88     738     777 
   Size:         88     650      39

  Fragments arranged by size: 

                 650      88      39
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     423     777 
   Size:        423     354
 
PfoI T'CCnGG_A

Cuts at:      0     445     777 
   Size:        445     332
 
PpuMI rG'GwC_Cy

Cuts at:      0      92     777 
   Size:         92     685
 
PspOMI G'GGCC_C

Cuts at:      0      84     182     609     734     777 
   Size:         84      98     427     125      43

  Fragments arranged by size: 

                 427     125      98      84      43
 
PstI C_TGCA'G

Cuts at:      0     309     579     608     777 
   Size:        309     270      29     169

  Fragments arranged by size: 

                 309     270     169      29
 
SanDI GG'GwC_CC

Cuts at:      0      92     777 
   Size:         92     685
 
SfcI C'TryA_G

Cuts at:      0     305     575     604     777 
   Size:        305     270      29     173

  Fragments arranged by size: 

                 305     270     173      29
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     328     777 
   Size:        328     449
 
SmaI CCC'GGG

Cuts at:      0     352     777 
   Size:        352     425
 
SmlI C'TyrA_G

Cuts at:      0     139     777 
   Size:        139     638
 
StuI AGG'CCT

Cuts at:      0     331     777 
   Size:        331     446
 
StyI C'CwwG_G

Cuts at:      0     527     627     777 
   Size:        527     100     150

  Fragments arranged by size: 

                 527     150     100
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     324     777 
   Size:        324     453
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     508     540     777 
   Size:        508      32     237

  Fragments arranged by size: 

                 508     237      32
 
XmaI C'CCGG_G

Cuts at:      0     350     777 
   Size:        350     427
 

 Enzymes that do cut:

     AarI     AlfI    AlwNI     ApaI     AvaI    AvrII     BaeI    BanII
     BcgI     BglI Bme1580I     BmrI     BpmI    BpuEI     BsaI    BsaXI
    BseRI    BseYI     BsgI Bsp1286I    BspMI    BsrBI    BsrFI     BtsI
     EaeI     EarI  Eco57MI    EcoNI EcoO109I     FspI  HindIII     MmeI
     MscI   MspA1I     NaeI   NgoMIV     NruI     NspI     PasI    PflMI
     PfoI    PpuMI   PspOMI     PstI    SanDI     SfcI     SfiI     SmaI
     SmlI     StuI     StyI    TaqII     TstI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    ApaLI     ApoI     AscI     AseI
    AsiSI    BamHI     BanI     BbeI     BbsI    BbvCI    BciVI     BclI
    BfrBI    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I    BsaAI
    BsaBI    BsaHI    BsaWI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoRI
    EcoRV     FalI     FseI    FspAI    HaeII    Hin4I   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MslI     NarI     NcoI     NdeI
     NheI     NotI     NsiI     PacI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI
  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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