Restriction map for pF1KE0152
##################################
# NO CUT ENZYMES (  202 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BfaI        BfrI        BglI        
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaHI       BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmFI       BsmI        Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BsrI        BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstF5I      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FauI        FokI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MaeI        MaeII       MaeIII      MfeI        
MluI        MluNI       MroI        MscI        MslI        
MspA1I      MunI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NlaIII      
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SalI        Sau96I      SbfI        ScaI        SexAI       
SfaNI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StyI        SwaI        
TaqI        Tsp45I      TspRI       Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


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# ONE CUT ENZYMES (   37 enzymes) #
###################################

AlwNI          52  CAGAAACTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaI          325  CCCGAG               C^[CT]CG[GA]G
BclI           59  TGATCA               T^GATCA
BcnI           80  CCCGG                CC^[GC]GG
BglII          93  AGATCT               A^GATCT
BsaBI          25  GATGACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          185  GAGACC               GGTCTC(1/5)
BsaJI         383  CCCAGG               C^C[ACGT][ACGT]GG
BseRI         137  CTCCTC               GAGGAG(10/8)
BsiYI         378  CCAAGCCCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          378  CCAAGCCCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         185  GAGAC                GTCTC(1/5)
BsoBI         325  CCCGAG               C^[CT]CG[GA]G
BstAPI        294  GCAGAAATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI         384  CCAGG                CC^[AT]GG
BstYI          93  AGATCT               [GA]^GATC[CT]
EaeI            2  TGGCCG               [CT]^GGCC[GA]
EarI          307  GAAGAG               CTCTTC(1/4)
EcoRII        384  CCAGG                ^CC[AT]GG
FbaI           59  TGATCA               T^GATCA
HapII          81  CCGG                 C^CGG
HpaII          81  CCGG                 C^CGG
Ksp632I       307  GAAGAG               CTCTTC(1/4)
MamI           25  GATGACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI           93  AGATCT               [GA]^GATC[CT]
MseI          253  TTAA                 T^TAA
MspI           81  CCGG                 C^CGG
MvaI          384  CCAGG                CC^[AT]GG
NciI           80  CCCGG                CC^[GC]GG
NlaIV          83  GGAACC               GG[ACGT]^[ACGT]CC
PleI          388  GAGTC                GAGTC(4/5)
SapI          307  GAAGAGC              GCTCTTC(1/4)
SmlI          286  CTTGAG               C^T[CT][GA]AG
StuI           46  AGGCCT               AGG^CCT
TfiI          117  GAATC                G^A[AT]TC
Tru9I         253  TTAA                 T^TAA
XhoII          93  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AluI          248  AGCT                 AG^CT
              278  AGCT                
BbvI          261  GCAGC                GCAGC(8/12)
              276  GCAGC               
BssKI          80  CCCGG                ^CC[ACGT]GG
              384  CCAGG               
DpnII          60  GATC                 ^GATC
               94  GATC                
Fnu4HI        261  GCAGC                GC^[ACGT]GC
              276  GCAGC               
HinfI         117  GAATC                G^A[ACGT]TC
              388  GAGTC               
HphI          165  GGTGA                GGTGA(8/7)
              339  GGTGA               
ItaI          261  GCAGC                GC^[ACGT]GC
              276  GCAGC               
MboI           60  GATC                 ^GATC
               94  GATC                
MwoI          294  GCAGAAATTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              303  GCAAGAAGAGC         
NdeII          60  GATC                 ^GATC
               94  GATC                
Sau3AI         60  GATC                 ^GATC
               94  GATC                
ScrFI          80  CCCGG                CC^[ACGT]GG
              384  CCAGG               
TseI          261  GCAGC                G^C[AT]GC
              276  GCAGC               
Tsp509I       155  AATT                 ^AATT
              299  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    4 enzymes) #
#########################################

DdeI        HaeIII      MboII       MnlI        
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