Restriction map for pF1KB7677
##################################
# NO CUT ENZYMES (  139 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AviII       BalI        BbeI        BbvCI       
BcgI        BciVI       BclI        BfrI        BglI        
BlpI        Bpu1102I    BsaBI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       HincII      
HindII      HpaI        KasI        KpnI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MunI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SalI        SapI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TfiI        Tth111I     XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AatII         310  GACGTC               GACGT^C
AflIII        686  ACGTGT               A^C[GA][CT]GT
AlwNI         786  CAGACTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          367  GGGCCC               GGGCC^C
AvrII          57  CCTAGG               C^CTAGG
BamHI         768  GGATCC               G^GATCC
BbrPI          67  CACGTG               CAC^GTG
BfaI           58  CTAG                 C^TAG
BglII         752  AGATCT               A^GATCT
BlnI           57  CCTAGG               C^CTAGG
BseRI         559  GAGGAG               GAGGAG(10/8)
BsmAI         837  GTCTC                GTCTC(1/5)
BssSI         839  CTCGTG               CACGAG(-5/-1)
BstAPI        180  GCACTGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DsaI          224  CCGCGG               C^C[GA][CT]GG
EaeI          513  CGGCCA               [CT]^GGCC[GA]
EarI          430  GAAGAG               CTCTTC(1/4)
HindIII       862  AAGCTT               A^AGCTT
Ksp632I       430  GAAGAG               CTCTTC(1/4)
KspI          224  CCGCGG               CCGC^GG
MaeI           58  CTAG                 C^TAG
PflMI         785  CCAGACTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI          67  CACGTG               CAC^GTG
PmlI           67  CACGTG               CAC^GTG
PpuMI         601  AGGACCC              [GA]G^G[AT]CC[CT]
PvuII         694  CAGCTG               CAG^CTG
SacI          589  GAGCTC               GAGCT^C
SacII         224  CCGCGG               CCGC^GG
ScaI          206  AGTACT               AGT^ACT
TaqI          239  TCGA                 T^CGA
TthHB8I       239  TCGA                 T^CGA
Van91I        785  CCAGACTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AfaI          207  GTAC                 GT^AC
              333  GTAC                
AspHI         229  GAGCAC               G[AT]GC[AT]^C
              589  GAGCTC              
BanI           61  GGCACC               G^G[CT][GA]CC
              287  GGCACC              
BsaAI          67  CACGTG               [CT]AC^GT[GA]
              685  TACGTG              
BsgI          178  CTGCAC               GTGCAG(16/14)
              584  GTGCAG              
BsiHKAI       229  GAGCAC               G[AT]GC[AT]^C
              589  GAGCTC              
BsmFI         421  GTCCC                GGGAC(10/14)
              564  GTCCC               
BspMI          98  ACCTGC               ACCTGC(4/8)
              473  ACCTGC              
DdeI          303  CTCAG                C^T[ACGT]AG
              449  CTCAG               
EcoNI          52  CCTCCCCTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              267  CCTCACCAAGG         
EcoT14I        57  CCTAGG               C^C[AT][AT]GG
              272  CCAAGG              
FauI          151  CCCGC                CCCGC(4/6)
              625  CCCGC               
MslI           63  CACCCACGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              517  CACCTGGATG          
PstI          478  CTGCAG               CTGCA^G
              597  CTGCAG              
RsaI          207  GTAC                 GT^AC
              333  GTAC                
SfcI          478  CTGCAG               C^T[GA][CT]AG
              597  CTGCAG              
StyI           57  CCTAGG               C^C[AT][AT]GG
              272  CCAAGG              
Tsp509I        14  AATT                 ^AATT
               93  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AlwI        
AvaII       BanII       BbsI        BbvI        BcnI        
BmyI        BpmI        BpuAI       BsaHI       BsaJI       
BsiYI       BslI        Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr13I      DpnII       DraII       EcoO109I    
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
Sau3AI      Sau96I      ScrFI       SfaNI       Tru9I       
TseI        Tsp45I      TspRI       XhoII       
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