Restriction map for pF1KB7677
 (Linear) (Six-base) MAPSORT of: KIBB7677.seq  Check: 9204  from: 1  to: 1023



KIBB7677.orf

 With 182 enzymes: SgfI * 

                        October 16, 2009 16:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     482    1023 
   Size:        482     541
 
AatII G_ACGT'C

Cuts at:      0     314    1023 
   Size:        314     709
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     372     415    1023 
   Size:        372      43     608

  Fragments arranged by size: 

                 608     372      43
 
AflIII A'CryG_T

Cuts at:      0     686    1023 
   Size:        686     337
 
AleI CACnn'nnGTG

Cuts at:      0      67    1023 
   Size:         67     956
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      85    1023 
   Size:         85     938
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     220     252    1023 
   Size:        220      32     771

  Fragments arranged by size: 

                 771     220      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     791    1023 
   Size:        791     232
 
ApaI G_GGCC'C

Cuts at:      0     371    1023 
   Size:        371     652
 
AvrII C'CTAG_G

Cuts at:      0      57    1023 
   Size:         57     966
 
BamHI G'GATC_C

Cuts at:      0     768    1023 
   Size:        768     255
 
BanI G'GyrC_C

Cuts at:      0      61     287    1023 
   Size:         61     226     736

  Fragments arranged by size: 

                 736     226      61
 
BanII G_rGCy'C

Cuts at:      0     357     371     593     722     782    1023 
   Size:        357      14     222     129      60     241

  Fragments arranged by size: 

                 357     241     222     129      60      14
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     141     403     743    1023 
   Size:        141     262     340     280

  Fragments arranged by size: 

                 340     280     262     141
 
BglII A'GATC_T

Cuts at:      0     752    1023 
   Size:        752     271
 
Bme1580I G_kGCm'C

Cuts at:      0     371     846    1023 
   Size:        371     475     177

  Fragments arranged by size: 

                 475     371     177
 
BmgBI CAC'GTC

Cuts at:      0     174    1023 
   Size:        174     849
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     313     401     976     985    1023 
   Size:        313      88     575       9      38

  Fragments arranged by size: 

                 575     313      88      38       9
 
Bpu10I CC'TnA_GC

Cuts at:      0     303    1023 
   Size:        303     720
 
BsaAI yAC'GTr

Cuts at:      0      69     687    1023 
   Size:         69     618     336

  Fragments arranged by size: 

                 618     336      69
 
BsaHI Gr'CG_yC

Cuts at:      0     311     400     746    1023 
   Size:        311      89     346     277

  Fragments arranged by size: 

                 346     311     277      89
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     220     250    1023 
   Size:        220      30     773

  Fragments arranged by size: 

                 773     220      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     574    1023 
   Size:        574     449
 
BseYI C'CCAG_C

Cuts at:      0     102     191     298     344     407    1023 
   Size:        102      89     107      46      63     616

  Fragments arranged by size: 

                 616     107     102      89      63      46
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     605    1023 
   Size:        163     442     418

  Fragments arranged by size: 

                 442     418     163
 
BsiHKAI G_wGCw'C

Cuts at:      0     233     593    1023 
   Size:        233     360     430

  Fragments arranged by size: 

                 430     360     233
 
Bsp1286I G_dGCh'C

Cuts at:      0     233     357     371     593     722     782     846    1023 
   Size:        233     124      14     222     129      60      64     177

  Fragments arranged by size: 

                 233     222     177     129     124      64      60      14
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     107     482    1023 
   Size:        107     375     541

  Fragments arranged by size: 

                 541     375     107
 
BssSI C'ACGA_G

Cuts at:      0     839    1023 
   Size:        839     184
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     186    1023 
   Size:        186     837
 
BstYI r'GATC_y

Cuts at:      0     116     383     752     768    1023 
   Size:        116     267     369      16     255

  Fragments arranged by size: 

                 369     267     255     116      16
 
BtgI C'CryG_G

Cuts at:      0     224    1023 
   Size:        224     799
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     643    1023 
   Size:        643     380
 
EaeI y'GGCC_r

Cuts at:      0     513    1023 
   Size:        513     510
 
EarI CTCTTCn'nnn_

Cuts at:      0     425    1023 
   Size:        425     598
 
EcoICRI GAG'CTC

Cuts at:      0     591    1023 
   Size:        591     432
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     313     372     401     415     976     985    1023 
   Size:        313      59      29      14     561       9      38

  Fragments arranged by size: 

                 561     313      59      38      29      14       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      56     271    1023 
   Size:         56     215     752

  Fragments arranged by size: 

                 752     215      56
 
EcoO109I rG'GnC_Cy

Cuts at:      0     368     602     639    1023 
   Size:        368     234      37     384

  Fragments arranged by size: 

                 384     368     234      37
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     297    1023 
   Size:        297     726
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     128     160     242     274     820     852    1023 
   Size:        128      32      82      32     546      32     171

  Fragments arranged by size: 

                 546     171     128      82      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0     862    1023 
   Size:        862     161
 
MslI CAynn'nnrTG

Cuts at:      0      67     521    1023 
   Size:         67     454     502

  Fragments arranged by size: 

                 502     454      67
 
MspA1I CmG'CkG

Cuts at:      0     226     348     696     963    1023 
   Size:        226     122     348     267      60

  Fragments arranged by size: 

                 348     267     226     122      60
 
PasI CC'CwG_GG

Cuts at:      0     493     606    1023 
   Size:        493     113     417

  Fragments arranged by size: 

                 493     417     113
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     791    1023 
   Size:        791     232
 
PfoI T'CCnGG_A

Cuts at:      0     921     935    1023 
   Size:        921      14      88

  Fragments arranged by size: 

                 921      88      14
 
PmlI CAC'GTG

Cuts at:      0      69    1023 
   Size:         69     954
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     220     252    1023 
   Size:        220      32     771

  Fragments arranged by size: 

                 771     220      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     602    1023 
   Size:        602     421
 
PsiI TTA'TAA

Cuts at:      0     860    1023 
   Size:        860     163
 
PspOMI G'GGCC_C

Cuts at:      0     367    1023 
   Size:        367     656
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     808     840    1023 
   Size:        808      32     183

  Fragments arranged by size: 

                 808     183      32
 
PstI C_TGCA'G

Cuts at:      0     482     601    1023 
   Size:        482     119     422

  Fragments arranged by size: 

                 482     422     119
 
PvuII CAG'CTG

Cuts at:      0     696    1023 
   Size:        696     327
 
SacI G_AGCT'C

Cuts at:      0     593    1023 
   Size:        593     430
 
SacII CC_GC'GG

Cuts at:      0     227    1023 
   Size:        227     796
 
ScaI AGT'ACT

Cuts at:      0     208    1023 
   Size:        208     815
 
SfcI C'TryA_G

Cuts at:      0     478     597    1023 
   Size:        478     119     426

  Fragments arranged by size: 

                 478     426     119
 
StyI C'CwwG_G

Cuts at:      0      57     272    1023 
   Size:         57     215     751

  Fragments arranged by size: 

                 751     215      57
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      79      94     421    1023 
   Size:         79      15     327     602

  Fragments arranged by size: 

                 602     327      79      15
 
TatI w'GTAC_w

Cuts at:      0     206     332    1023 
   Size:        206     126     691

  Fragments arranged by size: 

                 691     206     126
 
ZraI GAC'GTC

Cuts at:      0     312    1023 
   Size:        312     711
 

 Enzymes that do cut:

     AarI    AatII     AcuI   AflIII     AleI     AlfI     AloI    AlwNI
     ApaI    AvrII    BamHI     BanI    BanII     BbsI    BglII Bme1580I
    BmgBI     BpmI   Bpu10I    BsaAI    BsaHI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI Bsp1286I    BspMI    BssSI   BstAPI    BstYI     BtgI
    BtgZI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI
    Hin4I  HindIII     MslI   MspA1I     PasI    PflMI     PfoI     PmlI
     PpiI    PpuMI     PsiI   PspOMI     PsrI     PstI    PvuII     SacI
    SacII     ScaI     SfcI     StyI    TaqII     TatI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI     BaeI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI     BlpI     BmrI     BmtI
     BplI    BpuEI     BsaI    BsaBI    BsaWI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI     PmeI
    PshAI    PspXI     PvuI    RsrII     SalI    SanDI     SapI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI
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