Restriction map for pF1KB7738
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      ApaLI       
AscI        AseI        AsnI        Asp700I     AspEI       
AspHI       AvrII       BamHI       BbeI        BbrPI       
BbvCI       BcgI        BfrI        BglI        BlnI        
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmI        
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     ClaI        CpoI        
DraI        EagI        Eam1105I    EclXI       Eco52I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FseI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KspI        MamI        MfeI        MluI        
MroI        MunI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        SwaI        TfiI        XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccII          85  CGCG                 CG^CG
AflIII       1247  ACGTGT               A^C[GA][CT]GT
AocI         1120  CCTGAGG              CC^T[ACGT]AGG
Aor51HI        90  AGCGCT               AGC^GCT
ApaI          859  GGGCCC               GGGCC^C
AspI         1354  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         823  TGCGCA               TGC^GCA
BalI          519  TGGCCA               TGG^CCA
BbsI          550  GTCTTC               GAAGAC(2/6)
BciVI          22  GGATAC               GTATCC(6/5)
BclI          773  TGATCA               T^GATCA
BfaI          256  CTAG                 C^TAG
BglII         153  AGATCT               A^GATCT
BlpI         1182  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1182  GCTGAGC              GC^T[ACGT]AGC
BpuAI         550  GTCTTC               GAAGAC(2/6)
BsaAI        1246  TACGTG               [CT]AC^GT[GA]
BsmBI         184  GAGACG               CGTCTC(1/5)
Bsp1407I     1250  TGTACA               T^GTACA
BsrDI        1077  CATTGC               GCAATG(2/0)
BsrGI        1250  TGTACA               T^GTACA
BsrI          380  CCAGT                ACTGG(1/-1)
BstUI          85  CGCG                 CG^CG
BstXI        1419  CCAGCCATTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1120  CCTGAGG              CC^T[ACGT]AGG
CelII        1182  GCTGAGC              GC^T[ACGT]AGC
DraIII        816  CACCGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III       90  AGCGCT               AGC^GCT
Eco81I       1120  CCTGAGG              CC^T[ACGT]AGG
EcoNI         326  CCTGCAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          773  TGATCA               T^GATCA
FspI          823  TGCGCA               TGC^GCA
HgaI         1308  GCGTC                GACGC(5/10)
MaeI          256  CTAG                 C^TAG
MluNI         519  TGGCCA               TGG^CCA
MscI          519  TGGCCA               TGG^CCA
MseI          674  TTAA                 T^TAA
MslI         1372  CACTTGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI           85  CGCG                 CG^CG
PflFI        1354  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1133  CCAGGCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SmlI         1318  CTCAAG               C^T[CT][GA]AG
SspBI        1250  TGTACA               T^GTACA
Tru9I         674  TTAA                 T^TAA
Tth111I      1354  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1133  CCAGGCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AcsI          480  GAATTT               [GA]^AATT[CT]
              946  AAATTT              
AlwNI         559  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1134  CAGGCTCTG           
ApoI          480  GAATTT               [GA]^AATT[CT]
              946  AAATTT              
BpmI          378  CTCCAG               CTGGAG(16/14)
              866  CTCCAG              
BseRI        1123  GAGGAG               GAGGAG(10/8)
             1348  GAGGAG              
BsmAI         184  GAGAC                GTCTC(1/5)
              956  GAGAC               
BsrBI        1188  CCGCTC               CCGCTC(-3/-3)
                4  GAGCGG              
BsrFI        1157  ACCGGC               [GA]^CCGG[CT]
             1334  ACCGGC              
Cfr10I       1157  ACCGGC               [GA]^CCGG[CT]
             1334  ACCGGC              
DraII         859  GGGCCCT              [GA]G^G[ACGT]CC[CT]
             1274  AGGCCCT             
DrdI          805  GACTCAGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1333  GACCGGCCTGTC        
EaeI          519  TGGCCA               [CT]^GGCC[GA]
             1159  CGGCCA              
EarI          157  CTCTTC               CTCTTC(1/4)
             1279  CTCTTC              
EcoO109I      859  GGGCCCT              [GA]G^G[ACGT]CC[CT]
             1274  AGGCCCT             
HaeII          90  AGCGCT               [GA]GCGC^[CT]
             1007  AGCGCC              
Ksp632I       157  CTCTTC               CTCTTC(1/4)
             1279  CTCTTC              
MaeII         187  ACGT                 A^CGT
             1247  ACGT                
PstI          327  CTGCAG               CTGCA^G
             1021  CTGCAG              
PvuII         634  CAGCTG               CAG^CTG
             1024  CAGCTG              
SmaI          123  CCCGGG               CCC^GGG
              464  CCCGGG              
TaqI          143  TCGA                 T^CGA
              987  TCGA                
TthHB8I       143  TCGA                 T^CGA
              987  TCGA                
XcmI          507  CCAGCCCCACCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              513  CCACCCTGGCCATGG     
XmaCI         123  CCCGGG               C^CCGGG
              464  CCCGGG              
XmaI          123  CCCGGG               C^CCGGG
              464  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

Acc65I      AciI        AfaI        AluI        AlwI        
Asp718I     AvaI        AvaII       BanI        BanII       
BbvI        BcnI        BmyI        BsaJI       BsiYI       
BslI        BsmFI       BsoBI       Bsp1286I    BssKI       
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DsaI        EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        KpnI        MaeIII      MboI        
MboII       MflI        MnlI        MspA1I      MspI        
MvaI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TseI        Tsp45I      Tsp509I     TspRI       XhoII       
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