Restriction map for pF1KB7738
 (Linear) (Six-base) MAPSORT of: KIBB7738.seq  Check: 9261  from: 1  to: 1452



KIBB7738.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

Acc65I G'GTAC_C

Cuts at:      0     207     527     752    1452 
   Size:        207     320     225     700

  Fragments arranged by size: 

                 700     320     225     207
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      36     170     218    1452 
   Size:         36     134      48    1234

  Fragments arranged by size: 

                1234     134      48      36
 
AfeI AGC'GCT

Cuts at:      0      92    1452 
   Size:         92    1360
 
AflIII A'CryG_T

Cuts at:      0    1247    1452 
   Size:       1247     205
 
AlwNI CAG_nnn'CTG

Cuts at:      0     564    1139    1452 
   Size:        564     575     313

  Fragments arranged by size: 

                 575     564     313
 
ApaI G_GGCC'C

Cuts at:      0     863    1452 
   Size:        863     589
 
ApoI r'AATT_y

Cuts at:      0     480     946    1452 
   Size:        480     466     506

  Fragments arranged by size: 

                 506     480     466
 
AvaI C'yCGr_G

Cuts at:      0     123     464     532    1270    1452 
   Size:        123     341      68     738     182

  Fragments arranged by size: 

                 738     341     182     123      68
 
BanI G'GyrC_C

Cuts at:      0     207     430     499     527     752    1015    1452 
   Size:        207     223      69      28     225     263     437

  Fragments arranged by size: 

                 437     263     225     223     207      69      28
 
BanII G_rGCy'C

Cuts at:      0     130     296     647     863    1271    1387    1452 
   Size:        130     166     351     216     408     116      65

  Fragments arranged by size: 

                 408     351     216     166     130     116      65
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     543    1452 
   Size:        543     909
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      16    1452 
   Size:         16    1436
 
BclI T'GATC_A

Cuts at:      0     773    1452 
   Size:        773     679
 
BglII A'GATC_T

Cuts at:      0     153    1452 
   Size:        153    1299
 
BlpI GC'TnA_GC

Cuts at:      0    1183    1452 
   Size:       1183     269
 
Bme1580I G_kGCm'C

Cuts at:      0      44     435     749     863    1020    1452 
   Size:         44     391     314     114     157     432

  Fragments arranged by size: 

                 432     391     314     157     114      44
 
BmgBI CAC'GTC

Cuts at:      0     188    1452 
   Size:        188    1264
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     870    1452 
   Size:        870     582
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     363     851    1452 
   Size:        363     488     601

  Fragments arranged by size: 

                 601     488     363
 
Bpu10I CC'TnA_GC

Cuts at:      0     203    1452 
   Size:        203    1249
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1303    1452 
   Size:       1303     149
 
BsaAI yAC'GTr

Cuts at:      0    1248    1452 
   Size:       1248     204
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     112     142    1452 
   Size:        112      30    1310

  Fragments arranged by size: 

                1310     112      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1138    1363    1452 
   Size:       1138     225      89

  Fragments arranged by size: 

                1138     225      89
 
BseYI C'CCAG_C

Cuts at:      0     105     506     615     662    1418    1452 
   Size:        105     401     109      47     756      34

  Fragments arranged by size: 

                 756     401     109     105      47      34
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     178    1452 
   Size:        178    1274
 
Bsp1286I G_dGCh'C

Cuts at:      0      44     130     296     435     647     749     863    1020 
   Size:         44      86     166     139     212     102     114     157

Cuts at:   1020    1271    1387    1452 
   Size:        251     116      65

  Fragments arranged by size: 

                 251     212     166     157     139     116     114     102
                  86      65      44
 
BsrBI CCG'CTC

Cuts at:      0       6    1190    1452 
   Size:          6    1184     262

  Fragments arranged by size: 

                1184     262       6
 
BsrDI GCAATG_nn'

Cuts at:      0    1076    1452 
   Size:       1076     376
 
BsrFI r'CCGG_y

Cuts at:      0    1157    1334    1452 
   Size:       1157     177     118

  Fragments arranged by size: 

                1157     177     118
 
BsrGI T'GTAC_A

Cuts at:      0    1250    1452 
   Size:       1250     202
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1426    1452 
   Size:       1426      26
 
BstYI r'GATC_y

Cuts at:      0     153     165     490    1452 
   Size:        153      12     325     962

  Fragments arranged by size: 

                 962     325     153      12
 
Bsu36I CC'TnA_GG

Cuts at:      0    1121    1452 
   Size:       1121     331
 
BtgI C'CryG_G

Cuts at:      0     357     522     722     881    1452 
   Size:        357     165     200     159     571

  Fragments arranged by size: 

                 571     357     200     165     159
 
BtsI GCAGTG_nn'

Cuts at:      0     373    1452 
   Size:        373    1079
 
DraIII CAC_nnn'GTG

Cuts at:      0     821    1452 
   Size:        821     631
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     811    1339    1452 
   Size:        811     528     113

  Fragments arranged by size: 

                 811     528     113
 
EaeI y'GGCC_r

Cuts at:      0     519    1159    1452 
   Size:        519     640     293

  Fragments arranged by size: 

                 640     519     293
 
EarI CTCTTCn'nnn_

Cuts at:      0     163    1285    1452 
   Size:        163    1122     167

  Fragments arranged by size: 

                1122     167     163
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      36     170     218     363     851    1452 
   Size:         36     134      48     145     488     601

  Fragments arranged by size: 

                 601     488     145     134      48      36
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     330    1452 
   Size:        330    1122
 
EcoO109I rG'GnC_Cy

Cuts at:      0     860    1275    1452 
   Size:        860     415     177

  Fragments arranged by size: 

                 860     415     177
 
FspI TGC'GCA

Cuts at:      0     825    1452 
   Size:        825     627
 
FspAI rTGC'GCAy

Cuts at:      0     825    1452 
   Size:        825     627
 
HaeII r_GCGC'y

Cuts at:      0      94    1011    1452 
   Size:         94     917     441

  Fragments arranged by size: 

                 917     441      94
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     112     144    1452 
   Size:        112      32    1308

  Fragments arranged by size: 

                1308     112      32
 
KpnI G_GTAC'C

Cuts at:      0     211     531     756    1452 
   Size:        211     320     225     696

  Fragments arranged by size: 

                 696     320     225     211
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     620    1452 
   Size:        620     832
 
MscI TGG'CCA

Cuts at:      0     521    1452 
   Size:        521     931
 
MslI CAynn'nnrTG

Cuts at:      0    1376    1452 
   Size:       1376      76
 
MspA1I CmG'CkG

Cuts at:      0     105     636    1026    1452 
   Size:        105     531     390     426

  Fragments arranged by size: 

                 531     426     390     105
 
NcoI C'CATG_G

Cuts at:      0     357     522     881    1452 
   Size:        357     165     359     571

  Fragments arranged by size: 

                 571     359     357     165
 
PasI CC'CwG_GG

Cuts at:      0     757    1452 
   Size:        757     695
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1139    1452 
   Size:       1139     313
 
PfoI T'CCnGG_A

Cuts at:      0     161     179    1295    1452 
   Size:        161      18    1116     157

  Fragments arranged by size: 

                1116     161     157      18
 
PspOMI G'GGCC_C

Cuts at:      0     859    1452 
   Size:        859     593
 
PstI C_TGCA'G

Cuts at:      0     331    1025    1452 
   Size:        331     694     427

  Fragments arranged by size: 

                 694     427     331
 
PvuII CAG'CTG

Cuts at:      0     636    1026    1452 
   Size:        636     390     426

  Fragments arranged by size: 

                 636     426     390
 
SfcI C'TryA_G

Cuts at:      0     327     451    1021    1452 
   Size:        327     124     570     431

  Fragments arranged by size: 

                 570     431     327     124
 
SmaI CCC'GGG

Cuts at:      0     125     466    1452 
   Size:        125     341     986

  Fragments arranged by size: 

                 986     341     125
 
SmlI C'TyrA_G

Cuts at:      0    1318    1452 
   Size:       1318     134
 
StyI C'CwwG_G

Cuts at:      0     357     435     522     881    1433    1452 
   Size:        357      78      87     359     552      19

  Fragments arranged by size: 

                 552     359     357      87      78      19
 
TatI w'GTAC_w

Cuts at:      0    1250    1452 
   Size:       1250     202
 
Tth111I GACn'n_nGTC

Cuts at:      0    1357    1452 
   Size:       1357      95
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     514     520    1452 
   Size:        514       6     932

  Fragments arranged by size: 

                 932     514       6
 
XmaI C'CCGG_G

Cuts at:      0     123     464    1452 
   Size:        123     341     988

  Fragments arranged by size: 

                 988     341     123
 

 Enzymes that do cut:

   Acc65I     AcuI     AfeI   AflIII    AlwNI     ApaI     ApoI     AvaI
     BanI    BanII     BbsI    BciVI     BclI    BglII     BlpI Bme1580I
    BmgBI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaXI    BseRI
    BseYI    BsmBI Bsp1286I    BsrBI    BsrDI    BsrFI    BsrGI    BstXI
    BstYI   Bsu36I     BtgI     BtsI   DraIII     DrdI     EaeI     EarI
  Eco57MI    EcoNI EcoO109I     FspI    FspAI    HaeII    Hin4I     KpnI
     MmeI     MscI     MslI   MspA1I     NcoI     PasI    PflMI     PfoI
   PspOMI     PstI    PvuII     SfcI     SmaI     SmlI     StyI     TatI
  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI    BbvCI     BcgI    BfrBI     BglI     BmrI     BmtI
     BsaI    BsaBI    BsaHI    BsaWI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BspEI    BspHI    BspMI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI     EagI     EciI
  EcoICRI    EcoRI    EcoRV     FalI     FseI   HincII  HindIII     HpaI
     KasI     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TstI     XbaI     XhoI     XmnI
     ZraI
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