Restriction map for pF1KE0357
##################################
# NO CUT ENZYMES (  151 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AciI        
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
AspEI       AspHI       AspI        AviII       BalI        
BamHI       BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BlpI        BmyI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        Bsp1286I    BspDI       
BspEI       BspLU11I    BsrBI       BsrFI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     CelII       
Cfr10I      ClaI        CpoI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoNI       EcoO109I    
EcoO65I     EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HaeIII      HapII       
Hin1I       HpaI        HpaII       KasI        KspI        
MamI        MluI        MluNI       MroI        MscI        
MslI        MspI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SapI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
Tth111I     Van91I      XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

Acc65I        275  GGTACC               G^GTACC
AflIII       1278  ACGTGT               A^C[GA][CT]GT
AlwNI        1583  CAGAACCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1668  CCTCAGG              CC^T[ACGT]AGG
AseI          852  ATTAAT               AT^TAAT
AsnI          852  ATTAAT               AT^TAAT
Asp700I      1674  GAATTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       275  GGTACC               G^GTACC
AvaI          575  CTCGGG               C^[CT]CG[GA]G
AvrII        1555  CCTAGG               C^CTAGG
BanI          275  GGTACC               G^G[CT][GA]CC
BlnI         1555  CCTAGG               C^CTAGG
BseRI         572  CTCCTC               GAGGAG(10/8)
BsiYI         682  CCGTCACTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          682  CCGTCACTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         739  GAGAC                GTCTC(1/5)
BsoBI         575  CTCGGG               C^[CT]CG[GA]G
Bsp1407I     1221  TGTACA               T^GTACA
BspHI        1055  TCATGA               T^CATGA
BsrGI        1221  TGTACA               T^GTACA
BstF5I       1299  GGATG                GGATG(2/0)
Bsu36I       1668  CCTCAGG              CC^T[ACGT]AGG
Cac8I         829  GCAAGC               GC[ACGT]^[ACGT]GC
DraI          229  TTTAAA               TTT^AAA
Eco81I       1668  CCTCAGG              CC^T[ACGT]AGG
EcoRI         480  GAATTC               G^AATTC
FokI         1299  GGATG                GGATG(9/13)
HincII       1048  GTCAAC               GT[CT]^[GA]AC
HindII       1048  GTCAAC               GT[CT]^[GA]AC
HindIII      1335  AAGCTT               A^AGCTT
KpnI          275  GGTACC               GGTAC^C
MaeII        1278  ACGT                 A^CGT
MspA1I        551  CAGCTG               C[AC]G^C[GT]G
MwoI         1218  GCATGTACAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV         275  GGTACC               GG[ACGT]^[ACGT]CC
NspI         1218  GCATGT               [GA]CATG^[CT]
PshAI        1132  GACGCTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         852  ATTAAT               AT^TAAT
PstI         1589  CTGCAG               CTGCA^G
PvuII         551  CAGCTG               CAG^CTG
RcaI         1055  TCATGA               T^CATGA
ScaI         1760  AGTACT               AGT^ACT
SfcI         1589  CTGCAG               C^T[GA][CT]AG
SspBI        1221  TGTACA               T^GTACA
SwaI          228  ATTTAAAT             ATTT^AAAT
XbaI          484  TCTAGA               T^CTAGA
XmnI         1674  GAATTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcsI          480  GAATTC               [GA]^AATT[CT]
              489  AAATTC              
AlwI         1330  GATCC                GGATC(4/5)
             1042  GGATC               
ApoI          480  GAATTC               [GA]^AATT[CT]
              489  AAATTC              
AvaII         792  GGTCC                G^G[AT]CC
             1525  GGTCC               
BglII         316  AGATCT               A^GATCT
             1012  AGATCT              
BpmI          613  CTCCAG               CTGGAG(16/14)
              289  CTGGAG              
BsmFI         690  GGGAC                GGGAC(10/14)
              666  GTCCC               
BspMI         137  ACCTGC               ACCTGC(4/8)
             1587  ACCTGC              
BssKI         615  CCAGG                ^CC[ACGT]GG
              669  CCTGG               
BstNI         615  CCAGG                CC^[AT]GG
              669  CCTGG               
Cfr13I        792  GGTCC                G^G[ACGT]CC
             1525  GGTCC               
EcoRII        615  CCAGG                ^CC[AT]GG
              669  CCTGG               
EcoT14I        16  CCAAGG               C^C[AT][AT]GG
             1555  CCTAGG              
HgaI          692  GACGC                GACGC(5/10)
             1132  GACGC               
MfeI          521  CAATTG               C^AATTG
             1520  CAATTG              
MunI          521  CAATTG               C^AATTG
             1520  CAATTG              
MvaI          615  CCAGG                CC^[AT]GG
              669  CCTGG               
Sau96I        792  GGTCC                G^G[ACGT]CC
             1525  GGTCC               
ScrFI         615  CCAGG                CC^[ACGT]GG
              669  CCTGG               
SfaNI         991  GCATC                GCATC(5/9)
             1227  GCATC               
StyI           16  CCAAGG               C^C[AT][AT]GG
             1555  CCTAGG              
TaqI          504  TCGA                 T^CGA
             1130  TCGA                
TthHB8I       504  TCGA                 T^CGA
             1130  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   37 enzymes) #
#########################################

AfaI        AluI        BbvI        BfaI        BsaJI       
BsrDI       BsrI        BstYI       CfoI        DdeI        
DpnII       EarI        Fnu4HI      HhaI        HinP1I      
HinfI       HphI        ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MflI        MnlI        
MseI        NdeII       NlaIII      RsaI        Sau3AI      
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       XhoII       
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