Restriction map for pF1KE0036
##################################
# NO CUT ENZYMES (   94 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AflIII      Alw44I      AocI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspI        AviII       
AvrII       BamHI       BbeI        BbrPI       BbvCI       
BclI        BfrI        BglII       BlnI        BsaAI       
BsaBI       BsiWI       BsmI        BspDI       BspHI       
BspLU11I    BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      ClaI        DraI        
DraIII      DrdI        Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       FbaI        FseI        FspI        
HindIII     HpaI        KasI        KpnI        KspI        
MaeII       MamI        MluI        MseI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PflFI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RcaI        SacII       SalI        SbfI        ScaI        
SexAI       SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
SwaI        Tru9I       Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccIII        325  TCCGGA               T^CCGGA
AhdI          211  GACCTCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI         1037  GGGCCC               GGGCC^C
Asp700I      1914  GAACCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         211  GACCTCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI          861  GGCACC               G^G[CT][GA]CC
BbsI         1393  GTCTTC               GAAGAC(2/6)
BciVI        2108  GTATCC               GTATCC(6/5)
BfaI          478  CTAG                 C^TAG
BglI          727  GCCAGAGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI         1429  CTCCAG               CTGGAG(16/14)
BpuAI        1393  GTCTTC               GAAGAC(2/6)
BseAI         325  TCCGGA               T^CCGGA
BsgI         1060  CTGCAC               GTGCAG(16/14)
Bsp1407I     1241  TGTACA               T^GTACA
BspEI         325  TCCGGA               T^CCGGA
BsrDI         572  GCAATG               GCAATG(2/0)
BsrGI        1241  TGTACA               T^GTACA
BssHII       1096  GCGCGC               G^CGCGC
BstAPI       1100  GCATCCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         800  AGATCC               [GA]^GATC[CT]
CpoI          322  CGGTCCG              CG^G[AT]CCG
EagI         1032  CGGCCG               C^GGCCG
Eam1105I      211  GACCTCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1032  CGGCCG               C^GGCCG
Eco52I       1032  CGGCCG               C^GGCCG
HincII        751  GTTGAC               GT[CT]^[GA]AC
HindII        751  GTTGAC               GT[CT]^[GA]AC
MaeI          478  CTAG                 C^TAG
MfeI          153  CAATTG               C^AATTG
MflI          800  AGATCC               [GA]^GATC[CT]
MroI          325  TCCGGA               T^CCGGA
MunI          153  CAATTG               C^AATTG
PaeR7I       1880  CTCGAG               C^TCGAG
RsrII         322  CGGTCCG              CG^G[AT]CCG
SacI         1615  GAGCTC               GAGCT^C
SfiI          726  GGCCAGAGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI         1325  CCCGGG               CCC^GGG
SmlI         1880  CTCGAG               C^T[CT][GA]AG
SspBI        1241  TGTACA               T^GTACA
TfiI         1215  GAATC                G^A[AT]TC
XhoI         1880  CTCGAG               C^TCGAG
XhoII         800  AGATCC               [GA]^GATC[CT]
XmaCI        1325  CCCGGG               C^CCGGG
XmaI         1325  CCCGGG               C^CCGGG
XmnI         1914  GAACCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccII         909  CGCG                 CG^CG
             1097  CGCG                
AcyI          928  GACGCC               G[GA]^CG[CT]C
             1208  GACGCC              
AgeI          234  ACCGGT               A^CCGGT
             2035  ACCGGT              
AlwI          801  GATCC                GGATC(4/5)
             1523  GGATC               
Aor51HI       416  AGCGCT               AGC^GCT
             1016  AGCGCT              
BcgI          177  GCAGCGGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1474  GCACCCAACTCG        
BlpI         1764  GCTTAGC              GC^T[ACGT]AGC
             1858  GCTGAGC             
Bpu1102I     1764  GCTTAGC              GC^T[ACGT]AGC
             1858  GCTGAGC             
BsaHI         928  GACGCC               G[GA]^CG[CT]C
             1208  GACGCC              
BsiEI         648  CGACCG               CG[GA][CT]^CG
             1032  CGGCCG              
BsmBI        1206  GAGACG               CGTCTC(1/5)
             1934  GAGACG              
BsrBI         855  CCGCTC               CCGCTC(-3/-3)
             1089  CCGCTC              
BstUI         909  CGCG                 CG^CG
             1097  CGCG                
CelII        1764  GCTTAGC              GC^T[ACGT]AGC
             1858  GCTGAGC             
Eco47III      416  AGCGCT               AGC^GCT
             1016  AGCGCT              
EcoT14I       206  CCATGG               C^C[AT][AT]GG
             1271  CCATGG              
EcoT22I         1  ATGCAT               ATGCA^T
              277  ATGCAT              
FauI          514  CCCGC                CCCGC(4/6)
              818  CCCGC               
HgaI          928  GACGC                GACGC(5/10)
             1208  GACGC               
Hin1I         928  GACGCC               G[GA]^CG[CT]C
             1208  GACGCC              
MvnI          909  CGCG                 CG^CG
             1097  CGCG                
NcoI          206  CCATGG               C^CATGG
             1271  CCATGG              
NsiI            1  ATGCAT               ATGCA^T
              277  ATGCAT              
PinAI         234  ACCGGT               A^CCGGT
             2035  ACCGGT              
PleI          718  GAGTC                GAGTC(4/5)
              890  GAGTC               
PpuMI          26  GGGTCCT              [GA]G^G[AT]CC[CT]
              763  AGGACCT             
PshAI          96  GACCTCGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1069  GACCCCTGTC          
SapI          165  GAAGAGC              GCTCTTC(1/4)
             1925  GAAGAGC             
StyI          206  CCATGG               C^C[AT][AT]GG
             1271  CCATGG              


#########################################
# 3 OR MORE CUT ENZYMES (   89 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwNI       
ApoI        AspHI       AvaI        AvaII       BalI        
BanII       BbvI        BcnI        BmyI        BsaI        
BsaJI       BsaWI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstXI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoO109I    EcoRII      Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeIII      MboI        MboII       MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        TaqI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        
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