Restriction map for pF1KE0036
 (Linear) (Six-base) MAPSORT of: KIEE0036.seq  Check: 8848  from: 1  to: 2145



KIEE0036.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1108    2145 
   Size:       1108    1037
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2049    2145 
   Size:       2049      96
 
AfeI AGC'GCT

Cuts at:      0     418    1018    2145 
   Size:        418     600    1127

  Fragments arranged by size: 

                1127     600     418
 
AgeI A'CCGG_T

Cuts at:      0     234    2035    2145 
   Size:        234    1801     110

  Fragments arranged by size: 

                1801     234     110
 
AhdI GACnn_n'nnGTC

Cuts at:      0     216    2145 
   Size:        216    1929
 
AleI CACnn'nnGTG

Cuts at:      0     346    2145 
   Size:        346    1799
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     885    2145 
   Size:        885    1260
 
AlwNI CAG_nnn'CTG

Cuts at:      0     392     498    1949    2145 
   Size:        392     106    1451     196

  Fragments arranged by size: 

                1451     392     196     106
 
ApaI G_GGCC'C

Cuts at:      0    1041    2145 
   Size:       1041    1104
 
ApoI r'AATT_y

Cuts at:      0    1752    1809    2003    2145 
   Size:       1752      57     194     142

  Fragments arranged by size: 

                1752     194     142      57
 
AvaI C'yCGr_G

Cuts at:      0    1325    1880    1931    2084    2145 
   Size:       1325     555      51     153      61

  Fragments arranged by size: 

                1325     555     153      61      51
 
BanI G'GyrC_C

Cuts at:      0     861    2145 
   Size:        861    1284
 
BanII G_rGCy'C

Cuts at:      0     663    1041    1619    1739    1881    1932    2145 
   Size:        663     378     578     120     142      51     213

  Fragments arranged by size: 

                 663     578     378     213     142     120      51
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1386    2145 
   Size:       1386     759
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     166     200    1463    1497    2145 
   Size:        166      34    1263      34     648

  Fragments arranged by size: 

                1263     648     166      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2119    2145 
   Size:       2119      26
 
BfrBI ATG'CAT

Cuts at:      0       3     279    2145 
   Size:          3     276    1866

  Fragments arranged by size: 

                1866     276       3
 
BglI GCCn_nnn'nGGC

Cuts at:      0     733    2145 
   Size:        733    1412
 
BlpI GC'TnA_GC

Cuts at:      0    1765    1859    2145 
   Size:       1765      94     286

  Fragments arranged by size: 

                1765     286      94
 
Bme1580I G_kGCm'C

Cuts at:      0      67    1041    1349    2145 
   Size:         67     974     308     796

  Fragments arranged by size: 

                 974     796     308      67
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1414    2145 
   Size:       1414     731
 
Bpu10I CC'TnA_GC

Cuts at:      0    1996    2145 
   Size:       1996     149
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      88     462     960    2145 
   Size:         88     374     498    1185

  Fragments arranged by size: 

                1185     498     374      88
 
BsaHI Gr'CG_yC

Cuts at:      0     929    1209    2145 
   Size:        929     280     936

  Fragments arranged by size: 

                 936     929     280
 
BsaWI w'CCGG_w

Cuts at:      0     234     325     980    2035    2145 
   Size:        234      91     655    1055     110

  Fragments arranged by size: 

                1055     655     234     110      91
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      61      91    1453    1483    2145 
   Size:         61      30    1362      30     662

  Fragments arranged by size: 

                1362     662      61      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     480    1069    1659    2145 
   Size:        480     589     590     486

  Fragments arranged by size: 

                 590     589     486     480
 
BseYI C'CCAG_C

Cuts at:      0      12      50      72     317     559     773    1742    2145 
   Size:         12      38      22     245     242     214     969     403

  Fragments arranged by size: 

                 969     403     245     242     214      38      22      12
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1045    2145 
   Size:       1045    1100
 
BsiEI CG_ry'CG

Cuts at:      0     651    1035    2145 
   Size:        651     384    1110

  Fragments arranged by size: 

                1110     651     384
 
BsiHKAI G_wGCw'C

Cuts at:      0     399    1184    1619    2145 
   Size:        399     785     435     526

  Fragments arranged by size: 

                 785     526     435     399
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1200    1928    2145 
   Size:       1200     728     217

  Fragments arranged by size: 

                1200     728     217
 
Bsp1286I G_dGCh'C

Cuts at:      0      67     399     663    1041    1184    1349    1619    1739 
   Size:         67     332     264     378     143     165     270     120

Cuts at:   1739    1881    1932    2145 
   Size:        142      51     213

  Fragments arranged by size: 

                 378     332     270     264     213     165     143     142
                 120      67      51
 
BspEI T'CCGG_A

Cuts at:      0     325    2145 
   Size:        325    1820
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     775    1067    1108    2145 
   Size:        775     292      41    1037

  Fragments arranged by size: 

                1037     775     292      41
 
BsrBI CCG'CTC

Cuts at:      0     857    1091    2145 
   Size:        857     234    1054

  Fragments arranged by size: 

                1054     857     234
 
BsrDI GCAATG_nn'

Cuts at:      0     579    2145 
   Size:        579    1566
 
BsrFI r'CCGG_y

Cuts at:      0     234    1187    1625    2035    2145 
   Size:        234     953     438     410     110

  Fragments arranged by size: 

                 953     438     410     234     110
 
BsrGI T'GTAC_A

Cuts at:      0    1241    2145 
   Size:       1241     904
 
BssHII G'CGCG_C

Cuts at:      0    1096    2145 
   Size:       1096    1049
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1106    2145 
   Size:       1106    1039
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     135     207     388     993    1608    2145 
   Size:        135      72     181     605     615     537

  Fragments arranged by size: 

                 615     605     537     181     135      72
 
BstYI r'GATC_y

Cuts at:      0     800    2145 
   Size:        800    1345
 
BtgI C'CryG_G

Cuts at:      0     206     651    1271    1378    1695    2045    2145 
   Size:        206     445     620     107     317     350     100

  Fragments arranged by size: 

                 620     445     350     317     206     107     100
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1839    2145 
   Size:       1839     306
 
BtsI GCAGTG_nn'

Cuts at:      0      95     682     740     954    2145 
   Size:         95     587      58     214    1191

  Fragments arranged by size: 

                1191     587     214      95      58
 
EaeI y'GGCC_r

Cuts at:      0      32     654     725    1004    1032    1193    1385    1416 
   Size:         32     622      71     279      28     161     192      31

Cuts at:   1416    1568    2145 
   Size:        152     577

  Fragments arranged by size: 

                 622     577     279     192     161     152      71      32
                  31      28
 
EagI C'GGCC_G

Cuts at:      0    1032    2145 
   Size:       1032    1113
 
EarI CTCTTCn'nnn_

Cuts at:      0     160     296    1898    1920    2145 
   Size:        160     136    1602      22     225

  Fragments arranged by size: 

                1602     225     160     136      22
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1536    2145 
   Size:       1536     609
 
EcoICRI GAG'CTC

Cuts at:      0    1617    2145 
   Size:       1617     528
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1414    2049    2145 
   Size:       1414     635      96

  Fragments arranged by size: 

                1414     635      96
 
EcoO109I rG'GnC_Cy

Cuts at:      0      27     482     764    1038    1560    1888    1992    2145 
   Size:         27     455     282     274     522     328     104     153

  Fragments arranged by size: 

                 522     455     328     282     274     153     104      27
            
 
HaeII r_GCGC'y

Cuts at:      0     420    1020    1728    2145 
   Size:        420     600     708     417

  Fragments arranged by size: 

                 708     600     420     417
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      61      93     202     234     520     552    1114    1146 
   Size:         61      32     109      32     286      32     562      32

Cuts at:   1146    2060    2092    2145 
   Size:        914      32      53

  Fragments arranged by size: 

                 914     562     286     109      61      53      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0     753    2145 
   Size:        753    1392
 
MfeI C'AATT_G

Cuts at:      0     153    2145 
   Size:        153    1992
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     512     632     671    2145 
   Size:        512     120      39    1474

  Fragments arranged by size: 

                1474     512     120      39
 
MscI TGG'CCA

Cuts at:      0      34    1006    1195    1387    1418    1570    2145 
   Size:         34     972     189     192      31     152     575

  Fragments arranged by size: 

                 972     575     192     189     152      34      31
 
MslI CAynn'nnrTG

Cuts at:      0     346     670     867    1606    2145 
   Size:        346     324     197     739     539

  Fragments arranged by size: 

                 739     539     346     324     197
 
MspA1I CmG'CkG

Cuts at:      0     180     191     321     366     777    1517    2145 
   Size:        180      11     130      45     411     740     628

  Fragments arranged by size: 

                 740     628     411     180     130      45      11
 
NcoI C'CATG_G

Cuts at:      0     206    1271    2145 
   Size:        206    1065     874

  Fragments arranged by size: 

                1065     874     206
 
NsiI A_TGCA'T

Cuts at:      0       5     281    2145 
   Size:          5     276    1864

  Fragments arranged by size: 

                1864     276       5
 
NspI r_CATG'y

Cuts at:      0     283    1489    1680    2145 
   Size:        283    1206     191     465

  Fragments arranged by size: 

                1206     465     283     191
 
PasI CC'CwG_GG

Cuts at:      0      82    1341    2145 
   Size:         82    1259     804

  Fragments arranged by size: 

                1259     804      82
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     498     702    1791    2145 
   Size:        498     204    1089     354

  Fragments arranged by size: 

                1089     498     354     204
 
PfoI T'CCnGG_A

Cuts at:      0     401    1619    2145 
   Size:        401    1218     526

  Fragments arranged by size: 

                1218     526     401
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1903    1935    2145 
   Size:       1903      32     210

  Fragments arranged by size: 

                1903     210      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      27     764    2145 
   Size:         27     737    1381

  Fragments arranged by size: 

                1381     737      27
 
PshAI GACnn'nnGTC

Cuts at:      0     100    1073    2145 
   Size:        100     973    1072

  Fragments arranged by size: 

                1072     973     100
 
PspOMI G'GGCC_C

Cuts at:      0    1037    2145 
   Size:       1037    1108
 
PspXI vC'TCGA_Gb

Cuts at:      0    1880    2145 
   Size:       1880     265
 
PstI C_TGCA'G

Cuts at:      0     424     747    2104    2145 
   Size:        424     323    1357      41

  Fragments arranged by size: 

                1357     424     323      41
 
PvuII CAG'CTG

Cuts at:      0     366     777    1517    2145 
   Size:        366     411     740     628

  Fragments arranged by size: 

                 740     628     411     366
 
RsrII CG'GwC_CG

Cuts at:      0     323    2145 
   Size:        323    1822
 
SacI G_AGCT'C

Cuts at:      0    1619    2145 
   Size:       1619     526
 
SapI GCTCTTCn'nnn_

Cuts at:      0     160    1920    2145 
   Size:        160    1760     225

  Fragments arranged by size: 

                1760     225     160
 
SfcI C'TryA_G

Cuts at:      0     420     743    1350    2100    2145 
   Size:        420     323     607     750      45

  Fragments arranged by size: 

                 750     607     420     323      45
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     733    2145 
   Size:        733    1412
 
SmaI CCC'GGG

Cuts at:      0    1327    2145 
   Size:       1327     818
 
SmlI C'TyrA_G

Cuts at:      0    1880    2145 
   Size:       1880     265
 
SrfI GCCC'GGGC

Cuts at:      0    1327    2145 
   Size:       1327     818
 
StyI C'CwwG_G

Cuts at:      0     206    1271    2145 
   Size:        206    1065     874

  Fragments arranged by size: 

                1065     874     206
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      90     822    1491    2145 
   Size:         90     732     669     654

  Fragments arranged by size: 

                 732     669     654      90
 
TatI w'GTAC_w

Cuts at:      0    1241    2145 
   Size:       1241     904
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     135     495    1338    2145 
   Size:        135     360     843     807

  Fragments arranged by size: 

                 843     807     360     135
 
XhoI C'TCGA_G

Cuts at:      0    1880    2145 
   Size:       1880     265
 
XmaI C'CCGG_G

Cuts at:      0    1325    2145 
   Size:       1325     820
 
XmnI GAAnn'nnTTC

Cuts at:      0    1918    2145 
   Size:       1918     227
 

 Enzymes that do cut:

     AarI     AcuI     AfeI     AgeI     AhdI     AleI     AlfI    AlwNI
     ApaI     ApoI     AvaI     BanI    BanII     BbsI     BcgI    BciVI
    BfrBI     BglI     BlpI Bme1580I     BpmI   Bpu10I     BsaI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
   BstAPI    BstXI    BstYI     BtgI    BtgZI     BtsI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    HaeII    Hin4I   HincII
     MfeI     MmeI     MscI     MslI   MspA1I     NcoI     NsiI     NspI
     PasI    PflMI     PfoI     PpiI    PpuMI    PshAI   PspOMI    PspXI
     PstI    PvuII    RsrII     SacI     SapI     SfcI     SfiI     SmaI
     SmlI     SrfI     StyI    TaqII     TatI     XcmI     XhoI     XmaI
     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AloI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI
     BclI    BglII    BmgBI     BmrI     BmtI     BplI    BpuEI    BsaAI
    BsaBI    BsiWI     BsmI    BspHI    BssSI    BstBI   BstEII  BstZ17I
   Bsu36I     ClaI    CspCI     DraI   DraIII     DrdI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI     KasI
     KpnI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PciI     PmeI     PmlI     PsiI     PsrI     PvuI
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SspI     StuI     SwaI     TstI
  Tth111I     XbaI     ZraI
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