Restriction map for pF1KB6846
##################################
# NO CUT ENZYMES (  161 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        AlwI        
AocI        Aor51HI     ApaI        ApoI        AscI        
AseI        AsnI        Asp700I     AspEI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BbrPI       BbsI        BciVI       BclI        
BfaI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaI        
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiWI       BsmAI       BsmBI       BsmI        BsoBI       
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DpnII       
DraI        DraIII      EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HgaI        HindIII     HpaI        KspI        
MaeI        MaeII       MamI        MboI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MunI        MvnI        NaeI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        Sau3AI      ScaI        SexAI       
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        SspI        SwaI        TaqI        
TfiI        Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

Acc65I        122  GGTACC               G^GTACC
AccI          511  GTCTAC               GT^[AC][GT]AC
AcyI           79  GGCGCC               G[GA]^CG[CT]C
Alw44I          7  GTGCAC               G^TGCAC
ApaLI           7  GTGCAC               G^TGCAC
Asp718I       122  GGTACC               G^GTACC
AspHI           7  GTGCAC               G[AT]GC[AT]^C
BbeI           79  GGCGCC               GGCGC^C
BbvCI         455  CCTCAGC              CCTCAGC(-5/-2)
BcgI           12  CGAATGTCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          528  CCGGG                CC^[GC]GG
BglI          136  GCCAAGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI          79  GGCGCC               G[GA]^CG[CT]C
BsiHKAI         7  GTGCAC               G[AT]GC[AT]^C
Bsp1407I      545  TGTACA               T^GTACA
BsrGI         545  TGTACA               T^GTACA
BsrI           70  CCAGT                ACTGG(1/-1)
BstF5I        239  GGATG                GGATG(2/0)
CfoI           80  GCGC                 GCG^C
DdeI          456  CTCAG                C^T[ACGT]AG
DrdI          103  GACAGCCGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          340  CCGTGG               C^C[GA][CT]GG
EcoT14I       137  CCAAGG               C^C[AT][AT]GG
FauI          306  GCGGG                CCCGC(4/6)
FokI          239  GGATG                GGATG(9/13)
HaeII          79  GGCGCC               [GA]GCGC^[CT]
HapII         528  CCGG                 C^CGG
HhaI           80  GCGC                 GCG^C
Hin1I          79  GGCGCC               G[GA]^CG[CT]C
HinP1I         80  GCGC                 G^CGC
HincII        325  GTCAAC               GT[CT]^[GA]AC
HindII        325  GTCAAC               GT[CT]^[GA]AC
HpaII         528  CCGG                 C^CGG
HphI          395  TCACC                GGTGA(8/7)
KasI           79  GGCGCC               G^GCGCC
KpnI          122  GGTACC               GGTAC^C
MaeIII        101  GTGAC                ^GT[ACGT]AC
MseI          218  TTAA                 T^TAA
MspI          528  CCGG                 C^CGG
NarI           79  GGCGCC               GG^CGCC
NciI          528  CCGGG                CC^[GC]GG
NlaIII        361  CATG                 CATG^
NspI          360  GCATGT               [GA]CATG^[CT]
SbfI          560  CCTGCAGG             CCTGCA^GG
SfaNI         448  GATGC                GCATC(5/9)
SfiI          135  GGCCAAGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI           79  GGCGCC               GGC^GCC
Sse8387I      560  CCTGCAGG             CCTGCA^GG
SspBI         545  TGTACA               T^GTACA
StuI          557  AGGCCT               AGG^CCT
StyI          137  CCAAGG               C^C[AT][AT]GG
Tru9I         218  TTAA                 T^TAA
Tsp45I        101  GTGAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AfaI          123  GTAC                 GT^AC
              546  GTAC                
AlwNI         274  CAGGGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              313  CAGGCCCTG           
BanI           79  GGCGCC               G^G[CT][GA]CC
              122  GGTACC              
BpmI          443  CTCCAG               CTGGAG(16/14)
              115  CTGGAG              
Cac8I         255  GCAGGC               GC[ACGT]^[ACGT]GC
              559  GCCTGC              
DraII         275  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              314  AGGCCCT             
EcoNI         126  CCTCTTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              230  CCTGGAAGAGG         
EcoO109I      275  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              314  AGGCCCT             
HinfI         110  GAGTC                G^A[ACGT]TC
              509  GAGTC               
MspA1I        355  CAGCTG               C[AC]G^C[GT]G
              458  CAGCTG              
PleI          110  GAGTC                GAGTC(4/5)
              509  GAGTC               
PvuII         355  CAGCTG               CAG^CTG
              458  CAGCTG              
RsaI          123  GTAC                 GT^AC
              546  GTAC                
Tsp509I       220  AATT                 ^AATT
              520  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   30 enzymes) #
#########################################

AciI        AluI        BanII       BbvI        BmyI        
BsaJI       BsiYI       BslI        BsmFI       Bsp1286I    
BssKI       BstNI       Cfr13I      EarI        EcoRII      
Fnu4HI      HaeIII      ItaI        Ksp632I     MboII       
MnlI        MvaI        MwoI        NlaIV       PstI        
Sau96I      ScrFI       SfcI        TseI        TspRI       
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