Restriction map for pF1KB7317
##################################
# NO CUT ENZYMES (   82 enzymes) #
##################################

AatII       AclI        AflII       AgeI        Alw44I      
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp700I     AspHI       AspI        AvrII       BbrPI       
BclI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsiHKAI     BsiWI       Bsp1407I    BspDI       
BspLU11I    BsrGI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     CelII       ClaI        
DraI        DraIII      DrdI        Eco47III    EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
HindIII     HpaI        MfeI        MluI        MseI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        SacI        SapI        SbfI        SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SspBI       
SspI        SwaI        TfiI        Tru9I       Tth111I     
XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccI          111  GTCGAC               GT^[AC][GT]AC
AccIII       2750  TCCGGA               T^CCGGA
AcsI           96  GAATTT               [GA]^AATT[CT]
AhdI          955  GACCCGCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         848  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         2172  CCTGAGG              CC^T[ACGT]AGG
ApoI           96  GAATTT               [GA]^AATT[CT]
AspEI         955  GACCCGCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI        1901  GGATCC               G^GATCC
BbeI          277  GGCGCC               GGCGC^C
BbsI         1777  GTCTTC               GAAGAC(2/6)
BfaI         1898  CTAG                 C^TAG
BpuAI        1777  GTCTTC               GAAGAC(2/6)
BsaWI        2750  TCCGGA               [AT]^CCGG[AT]
BseAI        2750  TCCGGA               T^CCGGA
BsmI         2960  GCATTC               GAATGC(1/-1)
BspEI        2750  TCCGGA               T^CCGGA
BspHI         224  TCATGA               T^CATGA
Bsu36I       2172  CCTGAGG              CC^T[ACGT]AGG
CpoI          386  CGGACCG              CG^G[AT]CCG
Eam1105I      955  GACCCGCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1692  GAAGAG               CTCTTC(1/4)
Eco81I       2172  CCTGAGG              CC^T[ACGT]AGG
HaeII         277  GGCGCC               [GA]GCGC^[CT]
KasI          277  GGCGCC               G^GCGCC
Ksp632I      1692  GAAGAG               CTCTTC(1/4)
MaeI         1898  CTAG                 C^TAG
MroI         2750  TCCGGA               T^CCGGA
NarI          277  GGCGCC               GG^CGCC
PaeR7I       2095  CTCGAG               C^TCGAG
PstI          738  CTGCAG               CTGCA^G
RcaI          224  TCATGA               T^CATGA
RsrII         386  CGGACCG              CG^G[AT]CCG
SalI          111  GTCGAC               G^TCGAC
ScaI         1427  AGTACT               AGT^ACT
SfoI          277  GGCGCC               GGC^GCC
XhoI         2095  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

Acc65I       2561  GGTACC               G^GTACC
             2837  GGTACC              
Asp718I      2561  GGTACC               G^GTACC
             2837  GGTACC              
BciVI        2863  GGATAC               GTATCC(6/5)
              144  GTATCC              
BsaBI        1902  GATCCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2397  GATCCCCATC          
BsiEI          47  CGGCCG               CG[GA][CT]^CG
              446  CGGCCG              
BsrDI         441  CATTGC               GCAATG(2/0)
             2125  GCAATG              
BssHII         82  GCGCGC               G^CGCGC
             2910  GCGCGC              
BssSI         255  CACGAG               CACGAG(-5/-1)
              760  CTCGTG              
EagI           47  CGGCCG               C^GGCCG
              446  CGGCCG              
EclXI          47  CGGCCG               C^GGCCG
              446  CGGCCG              
Eco52I         47  CGGCCG               C^GGCCG
              446  CGGCCG              
KpnI         2561  GGTACC               GGTAC^C
             2837  GGTACC              
KspI           50  CCGCGG               CCGC^GG
               77  CCGCGG              
MamI         1902  GATCCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2397  GATCCCCATC          
PshAI        1359  GACCAGCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1623  GACCATTGTC          
SacII          50  CCGCGG               CCGC^GG
               77  CCGCGG              
SexAI         209  ACCAGGT              A^CC[AT]GGT
             2312  ACCTGGT             
SfcI          738  CTGCAG               C^T[GA][CT]AG
             1707  CTACAG              
SmaI           11  CCCGGG               CCC^GGG
             1800  CCCGGG              
StuI         1715  AGGCCT               AGG^CCT
             1892  AGGCCT              
Tsp509I        97  AATT                 ^AATT
             2069  AATT                
XmaCI          11  CCCGGG               C^CCGGG
             1800  CCCGGG              
XmaI           11  CCCGGG               C^CCGGG
             1800  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  116 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwI        ApaI        AvaI        AvaII       
AviII       BalI        BanI        BanII       BbvCI       
BbvI        BcgI        BcnI        BglI        BmyI        
BpmI        BsaAI       BsaHI       BsaI        BsaJI       
BseRI       BsgI        BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
FspI        HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NciI        NcoI        
NdeII       NgoMIV      NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfiI        
SmlI        SphI        StyI        TaqI        TseI        
Tsp45I      TspRI       TthHB8I     Van91I      XcmI        
XhoII       
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