Restriction map for pF1KB7317
 (Linear) (Six-base) MAPSORT of: KIBB7317.seq  Check: 1232  from: 1  to: 3015



KIBB7317.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      76     358    2717    3015 
   Size:         76     282    2359     298

  Fragments arranged by size: 

                2359     298     282      76
 
AccI GT'mk_AC

Cuts at:      0     112    3015 
   Size:        112    2903
 
Acc65I G'GTAC_C

Cuts at:      0    2561    2837    3015 
   Size:       2561     276     178

  Fragments arranged by size: 

                2561     276     178
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1551    3015 
   Size:       1551    1464
 
AflIII A'CryG_T

Cuts at:      0     107     203    1523    2459    3015 
   Size:        107      96    1320     936     556

  Fragments arranged by size: 

                1320     936     556     107      96
 
AhdI GACnn_n'nnGTC

Cuts at:      0     960    3015 
   Size:        960    2055
 
AleI CACnn'nnGTG

Cuts at:      0      70     505    3015 
   Size:         70     435    2510

  Fragments arranged by size: 

                2510     435      70
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     346    3015 
   Size:        346    2669
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     991    1023    3015 
   Size:        991      32    1992

  Fragments arranged by size: 

                1992     991      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     853    3015 
   Size:        853    2162
 
ApaI G_GGCC'C

Cuts at:      0    1047    2979    3002    3015 
   Size:       1047    1932      23      13

  Fragments arranged by size: 

                1932    1047      23      13
 
ApoI r'AATT_y

Cuts at:      0      96    3015 
   Size:         96    2919
 
AvaI C'yCGr_G

Cuts at:      0      11     269    1267    1387    1800    1880    2095    3015 
   Size:         11     258     998     120     413      80     215     920

  Fragments arranged by size: 

                 998     920     413     258     215     120      80      11
            
 
BamHI G'GATC_C

Cuts at:      0    1901    3015 
   Size:       1901    1114
 
BanI G'GyrC_C

Cuts at:      0     277     346     466     565     990    1144    1513    1764 
   Size:        277      69     120      99     425     154     369     251

Cuts at:   1764    1965    2233    2561    2729    2837    3008    3015 
   Size:        201     268     328     168     108     171       7

  Fragments arranged by size: 

                 425     369     328     277     268     251     201     171
                 168     154     120     108      99      69       7
 
BanII G_rGCy'C

Cuts at:      0     232    1047    1267    1274    1388    1542    1820    2342 
   Size:        232     815     220       7     114     154     278     522

Cuts at:   2342    2979    3002    3015 
   Size:        637      23      13

  Fragments arranged by size: 

                 815     637     522     278     232     220     154     114
                  23      13       7
 
BbeI G_GCGC'C

Cuts at:      0     281    3015 
   Size:        281    2734
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1770    3015 
   Size:       1770    1245
 
BbvCI CC'TCA_GC

Cuts at:      0     526     931     966    1548    3015 
   Size:        526     405      35     582    1467

  Fragments arranged by size: 

                1467     582     526     405      35
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     424     458    2350    2384    2629    2663    3015 
   Size:        424      34    1892      34     245      34     352

  Fragments arranged by size: 

                1892     424     352     245      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     155    2857    3015 
   Size:        155    2702     158

  Fragments arranged by size: 

                2702     158     155
 
BglI GCCn_nnn'nGGC

Cuts at:      0      55     574     820    1865    1999    2422    3006    3015 
   Size:         55     519     246    1045     134     423     584       9

  Fragments arranged by size: 

                1045     584     519     423     246     134      55       9
            
 
Bme1580I G_kGCm'C

Cuts at:      0     349     613     767     873    1047    1116    1160    1516 
   Size:        349     264     154     106     174      69      44     356

Cuts at:   1516    1988    2734    2979    3002    3015 
   Size:        472     746     245      23      13

  Fragments arranged by size: 

                 746     472     356     349     264     245     174     154
                 106      69      44      23      13
 
BmgBI CAC'GTC

Cuts at:      0     110    2460    2907    3015 
   Size:        110    2350     447     108

  Fragments arranged by size: 

                2350     447     110     108
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     138    1815    3015 
   Size:        138    1677    1200

  Fragments arranged by size: 

                1677    1200     138
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2172    3015 
   Size:       2172     843
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     400     887     993    1057    1432    1949    2374    3015 
   Size:        400     487     106      64     375     517     425     641

  Fragments arranged by size: 

                 641     517     487     425     400     375     106      64
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     526     931     966    1261    1548    2044    3015 
   Size:        526     405      35     295     287     496     971

  Fragments arranged by size: 

                 971     526     496     405     295     287      35
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     475    1453    1489    3015 
   Size:        475     978      36    1526

  Fragments arranged by size: 

                1526     978     475      36
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     352    1582    2270    2837    3015 
   Size:        352    1230     688     567     178

  Fragments arranged by size: 

                1230     688     567     352     178
 
BsaAI yAC'GTr

Cuts at:      0     143     206    1524    3015 
   Size:        143      63    1318    1491

  Fragments arranged by size: 

                1491    1318     143      63
 
BsaBI GATnn'nnATC

Cuts at:      0    1906    2401    3015 
   Size:       1906     495     614

  Fragments arranged by size: 

                1906     614     495
 
BsaHI Gr'CG_yC

Cuts at:      0     278     486    2053    3015 
   Size:        278     208    1567     962

  Fragments arranged by size: 

                1567     962     278     208
 
BsaWI w'CCGG_w

Cuts at:      0    2750    3015 
   Size:       2750     265
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     739     769     877     907     991    1021    2545    2575 
   Size:        739      30     108      30      84      30    1524      30

Cuts at:   2575    3015 
   Size:        440

  Fragments arranged by size: 

                1524     739     440     108      84      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     727     814    1750    1864    2240    2436    2569    2859 
   Size:        727      87     936     114     376     196     133     290

Cuts at:   2859    3015 
   Size:        156

  Fragments arranged by size: 

                 936     727     376     290     196     156     133     114
                  87
 
BseYI C'CCAG_C

Cuts at:      0     260     342     831    1160    2978    3015 
   Size:        260      82     489     329    1818      37

  Fragments arranged by size: 

                1818     489     329     260      82      37
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     804     957     965    1198    2584    3015 
   Size:        804     153       8     233    1386     431

  Fragments arranged by size: 

                1386     804     431     233     153       8
 
BsiEI CG_ry'CG

Cuts at:      0      50     449    3015 
   Size:         50     399    2566

  Fragments arranged by size: 

                2566     399      50
 
BsmI GAATG_Cn'

Cuts at:      0    2960    3015 
   Size:       2960      55
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     266     493    1371    1500    3015 
   Size:        266     227     878     129    1515

  Fragments arranged by size: 

                1515     878     266     227     129
 
Bsp1286I G_dGCh'C

Cuts at:      0     232     349     613     767     873    1047    1116    1160 
   Size:        232     117     264     154     106     174      69      44

Cuts at:   1160    1267    1274    1388    1516    1542    1820    1988    2342 
   Size:        107       7     114     128      26     278     168     354

Cuts at:   2342    2734    2979    3002    3015 
   Size:        392     245      23      13

  Fragments arranged by size: 

                 392     354     278     264     245     232     174     168
                 154     128     117     114     107     106      69      44
                  26      23      13       7
 
BspEI T'CCGG_A

Cuts at:      0    2750    3015 
   Size:       2750     265
 
BspHI T'CATG_A

Cuts at:      0     224    3015 
   Size:        224    2791
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      76     358    1211    2717    3015 
   Size:         76     282     853    1506     298

  Fragments arranged by size: 

                1506     853     298     282      76
 
BsrBI CCG'CTC

Cuts at:      0     717     804    1142    1347    2508    3015 
   Size:        717      87     338     205    1161     507

  Fragments arranged by size: 

                1161     717     507     338     205      87
 
BsrDI GCAATG_nn'

Cuts at:      0     440    2132    3015 
   Size:        440    1692     883

  Fragments arranged by size: 

                1692     883     440
 
BsrFI r'CCGG_y

Cuts at:      0     790    2011    2236    2834    3005    3015 
   Size:        790    1221     225     598     171      10

  Fragments arranged by size: 

                1221     790     598     225     171      10
 
BssHII G'CGCG_C

Cuts at:      0      82    2910    3015 
   Size:         82    2828     105

  Fragments arranged by size: 

                2828     105      82
 
BssSI C'ACGA_G

Cuts at:      0     255     760    3015 
   Size:        255     505    2255

  Fragments arranged by size: 

                2255     505     255
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1191    1206    1584    2829    3015 
   Size:       1191      15     378    1245     186

  Fragments arranged by size: 

                1245    1191     378     186      15
 
BstYI r'GATC_y

Cuts at:      0    1634    1901    2396    2948    3015 
   Size:       1634     267     495     552      67

  Fragments arranged by size: 

                1634     552     495     267      67
 
Bsu36I CC'TnA_GG

Cuts at:      0    2173    3015 
   Size:       2173     842
 
BtgI C'CryG_G

Cuts at:      0      50      77     120     617    1577    1988    2112    2384 
   Size:         50      27      43     497     960     411     124     272

Cuts at:   2384    2579    2801    3015 
   Size:        195     222     214

  Fragments arranged by size: 

                 960     497     411     272     222     214     195     124
                  50      43      27
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2412    3015 
   Size:       2412     603
 
BtsI GCAGTG_nn'

Cuts at:      0     519     656     792    1077    3015 
   Size:        519     137     136     285    1938

  Fragments arranged by size: 

                1938     519     285     137     136
 
EaeI y'GGCC_r

Cuts at:      0      47      62     446     575     979    1065    1196    1560 
   Size:         47      15     384     129     404      86     131     364

Cuts at:   1560    1991    2115    2282    2450    3015 
   Size:        431     124     167     168     565

  Fragments arranged by size: 

                 565     431     404     384     364     168     167     131
                 129     124      86      47      15
 
EagI C'GGCC_G

Cuts at:      0      47     446    3015 
   Size:         47     399    2569

  Fragments arranged by size: 

                2569     399      47
 
EarI CTCTTCn'nnn_

Cuts at:      0    1687    3015 
   Size:       1687    1328
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2080    2876    3015 
   Size:       2080     796     139

  Fragments arranged by size: 

                2080     796     139
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     400     887     993    1057    1432    1551    1949    2374 
   Size:        400     487     106      64     375     119     398     425

Cuts at:   2374    3015 
   Size:        641

  Fragments arranged by size: 

                 641     487     425     400     398     375     119     106
                  64
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1177    2047    2172    3015 
   Size:       1177     870     125     843

  Fragments arranged by size: 

                1177     870     843     125
 
EcoO109I rG'GnC_Cy

Cuts at:      0     264     520    1044    2001    2177    2793    2998    2999 
   Size:        264     256     524     957     176     616     205       1

Cuts at:   2999    3015 
   Size:         16

  Fragments arranged by size: 

                 957     616     524     264     256     205     176      16
                   1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     378     555    3015 
   Size:        378     177    2460

  Fragments arranged by size: 

                2460     378     177
 
FspI TGC'GCA

Cuts at:      0     253     511     625    3015 
   Size:        253     258     114    2390

  Fragments arranged by size: 

                2390     258     253     114
 
HaeII r_GCGC'y

Cuts at:      0     281    3015 
   Size:        281    2734
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     655     687     946     978    1478    1510    1734    1766 
   Size:        655      32     259      32     500      32     224      32

Cuts at:   1766    3015 
   Size:       1249

  Fragments arranged by size: 

                1249     655     500     259     224      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     113    1293    2304    3015 
   Size:        113    1180    1011     711

  Fragments arranged by size: 

                1180    1011     711     113
 
KasI G'GCGC_C

Cuts at:      0     277    3015 
   Size:        277    2738
 
KpnI G_GTAC'C

Cuts at:      0    2565    2841    3015 
   Size:       2565     276     174

  Fragments arranged by size: 

                2565     276     174
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     365    1280    3015 
   Size:        365     915    1735

  Fragments arranged by size: 

                1735     915     365
 
MscI TGG'CCA

Cuts at:      0     577    1198    1993    3015 
   Size:        577     621     795    1022

  Fragments arranged by size: 

                1022     795     621     577
 
MslI CAynn'nnrTG

Cuts at:      0      70     505    1582    2200    2827    2929    3015 
   Size:         70     435    1077     618     627     102      86

  Fragments arranged by size: 

                1077     627     618     435     102      86      70
 
MspA1I CmG'CkG

Cuts at:      0      52      79     864     875    1103    1124    1285    1977 
   Size:         52      27     785      11     228      21     161     692

Cuts at:   1977    2208    2408    2643    2780    2982    3015 
   Size:        231     200     235     137     202      33

  Fragments arranged by size: 

                 785     692     235     231     228     202     200     161
                 137      52      33      27      21      11
 
NaeI GCC'GGC

Cuts at:      0     792    2013    2238    3007    3015 
   Size:        792    1221     225     769       8

  Fragments arranged by size: 

                1221     792     769     225       8
 
NarI GG'CG_CC

Cuts at:      0     278    3015 
   Size:        278    2737
 
NcoI C'CATG_G

Cuts at:      0     617    1577    2579    3015 
   Size:        617     960    1002     436

  Fragments arranged by size: 

                1002     960     617     436
 
NgoMIV G'CCGG_C

Cuts at:      0     790    2011    2236    3005    3015 
   Size:        790    1221     225     769      10

  Fragments arranged by size: 

                1221     790     769     225      10
 
NspI r_CATG'y

Cuts at:      0     333    1136    2220    2244    3015 
   Size:        333     803    1084      24     771

  Fragments arranged by size: 

                1084     803     771     333      24
 
PasI CC'CwG_GG

Cuts at:      0     853    1109    2916    2994    3015 
   Size:        853     256    1807      78      21

  Fragments arranged by size: 

                1807     853     256      78      21
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1329    1578    1631    2408    3015 
   Size:       1329     249      53     777     607

  Fragments arranged by size: 

                1329     777     607     249      53
 
PfoI T'CCnGG_A

Cuts at:      0     554    1409    1721    3015 
   Size:        554     855     312    1294

  Fragments arranged by size: 

                1294     855     554     312
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     991    1023    1807    1839    3015 
   Size:        991      32     784      32    1176

  Fragments arranged by size: 

                1176     991     784      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     264     520    2177    3015 
   Size:        264     256    1657     838

  Fragments arranged by size: 

                1657     838     264     256
 
PshAI GACnn'nnGTC

Cuts at:      0    1363    1627    3015 
   Size:       1363     264    1388

  Fragments arranged by size: 

                1388    1363     264
 
PspOMI G'GGCC_C

Cuts at:      0    1043    2975    2998    3015 
   Size:       1043    1932      23      17

  Fragments arranged by size: 

                1932    1043      23      17
 
PspXI vC'TCGA_Gb

Cuts at:      0    2095    3015 
   Size:       2095     920
 
PstI C_TGCA'G

Cuts at:      0     742    3015 
   Size:        742    2273
 
PvuII CAG'CTG

Cuts at:      0     864    2208    2982    3015 
   Size:        864    1344     774      33

  Fragments arranged by size: 

                1344     864     774      33
 
RsrII CG'GwC_CG

Cuts at:      0     387    3015 
   Size:        387    2628
 
SacII CC_GC'GG

Cuts at:      0      53      80    3015 
   Size:         53      27    2935

  Fragments arranged by size: 

                2935      53      27
 
SalI G'TCGA_C

Cuts at:      0     111    3015 
   Size:        111    2904
 
SanDI GG'GwC_CC

Cuts at:      0     264     520    3015 
   Size:        264     256    2495

  Fragments arranged by size: 

                2495     264     256
 
ScaI AGT'ACT

Cuts at:      0    1429    3015 
   Size:       1429    1586
 
SexAI A'CCwGG_T

Cuts at:      0     209    2312    3015 
   Size:        209    2103     703

  Fragments arranged by size: 

                2103     703     209
 
SfcI C'TryA_G

Cuts at:      0     738    1707    3015 
   Size:        738     969    1308

  Fragments arranged by size: 

                1308     969     738
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1865    1999    2422    3015 
   Size:       1865     134     423     593

  Fragments arranged by size: 

                1865     593     423     134
 
SfoI GGC'GCC

Cuts at:      0     279    3015 
   Size:        279    2736
 
SmaI CCC'GGG

Cuts at:      0      13    1802    3015 
   Size:         13    1789    1213

  Fragments arranged by size: 

                1789    1213      13
 
SmlI C'TyrA_G

Cuts at:      0     490    1468    1504    2095    3015 
   Size:        490     978      36     591     920

  Fragments arranged by size: 

                 978     920     591     490      36
 
SphI G_CATG'C

Cuts at:      0     333    2220    2244    3015 
   Size:        333    1887      24     771

  Fragments arranged by size: 

                1887     771     333      24
 
StuI AGG'CCT

Cuts at:      0    1717    1894    3015 
   Size:       1717     177    1121

  Fragments arranged by size: 

                1717    1121     177
 
StyI C'CwwG_G

Cuts at:      0     474     617    1577    2579    3015 
   Size:        474     143     960    1002     436

  Fragments arranged by size: 

                1002     960     474     436     143
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     146     741    2221    2592    3007    3015 
   Size:        146     595    1480     371     415       8

  Fragments arranged by size: 

                1480     595     415     371     146       8
 
TatI w'GTAC_w

Cuts at:      0     734    1427    2141    3015 
   Size:        734     693     714     874

  Fragments arranged by size: 

                 874     734     714     693
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     739     771     850     877     882     909    1763    1795 
   Size:        739      32      79      27       5      27     854      32

Cuts at:   1795    3015 
   Size:       1220

  Fragments arranged by size: 

                1220     854     739      79      32      32      27      27
                   5
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     174    1191    2205    3015 
   Size:        174    1017    1014     810

  Fragments arranged by size: 

                1017    1014     810     174
 
XhoI C'TCGA_G

Cuts at:      0    2095    3015 
   Size:       2095     920
 
XmaI C'CCGG_G

Cuts at:      0      11    1800    3015 
   Size:         11    1789    1215

  Fragments arranged by size: 

                1789    1215      11
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI   AflIII     AhdI     AleI     AlfI
     AloI    AlwNI     ApaI     ApoI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BglI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI
    PflMI     PfoI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PstI
    PvuII    RsrII    SacII     SalI    SanDI     ScaI    SexAI     SfcI
     SfiI     SfoI     SmaI     SmlI     SphI     StuI     StyI    TaqII
     TatI     TstI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII     AgeI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI     BclI    BfrBI    BglII     BlpI     BmtI
  BsiHKAI    BsiWI    BsrGI   BstAPI    BstBI   BstEII  BstZ17I     ClaI
    CspCI     DraI   DraIII     DrdI  EcoICRI    EcoRI    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     MluI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PciI     PmeI     PmlI     PsiI     PsrI
     PvuI     SacI     SapI     SbfI     SgfI    SgrAI    SnaBI     SpeI
     SrfI     SspI     SwaI  Tth111I     XbaI     XmnI     ZraI
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