Restriction map for pF1KA0325
 (Linear) (Six-base) MAPSORT of: KIAA0325.seq  Check: 9024  from: 1  to: 13938



KIAA0325.orf

 With 182 enzymes: SgfI * 

                        May 20, 2014 13:33  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     107    3693    3948    4685    5910   11943   12263   13938 
   Size:        107    3586     255     737    1225    6033     320    1675

  Fragments arranged by size: 

                6033    3586    1675    1225     737     320     255     107
            
 
AatII G_ACGT'C

Cuts at:      0     263   13938 
   Size:        263   13675
 
AccI GT'mk_AC

Cuts at:      0    3473    4634    8651    9635   12863   13826   13938 
   Size:       3473    1161    4017     984    3228     963     112

  Fragments arranged by size: 

                4017    3473    3228    1161     984     963     112
 
Acc65I G'GTAC_C

Cuts at:      0    3008   13938 
   Size:       3008   10930
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     524    1273    1357    2065    2810    3148    3155    3814 
   Size:        524     749      84     708     745     338       7     659

Cuts at:   3814    3889    6206    6456    6565    6733    7800    7915    8584 
   Size:         75    2317     250     109     168    1067     115     669

Cuts at:   8584    8710   10456   10839   11338   11557   11695   12307   13051 
   Size:        126    1746     383     499     219     138     612     744

Cuts at:  13051   13255   13428   13938 
   Size:        204     173     510

  Fragments arranged by size: 

                2317    1746    1067     749     745     744     708     669
                 659     612     524     510     499     383     338     250
                 219     204     173     168     138     126     115     109
                  84      75       7
 
AfeI AGC'GCT

Cuts at:      0     241     925    2503    3583    7462    8426   13938 
   Size:        241     684    1578    1080    3879     964    5512

  Fragments arranged by size: 

                5512    3879    1578    1080     964     684     241
 
AflII C'TTAA_G

Cuts at:      0    2197   13938 
   Size:       2197   11741
 
AflIII A'CryG_T

Cuts at:      0      77    6488    6843   10112   10211   12017   12159   12659 
   Size:         77    6411     355    3269      99    1806     142     500

Cuts at:  12659   13523   13938 
   Size:        864     415

  Fragments arranged by size: 

                6411    3269    1806     864     500     415     355     142
                  99      77
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1848   12636   13104   13938 
   Size:       1848   10788     468     834

  Fragments arranged by size: 

               10788    1848     834     468
 
AleI CACnn'nnGTG

Cuts at:      0    1790    1913    3514    6515    6844    7567   12100   12597 
   Size:       1790     123    1601    3001     329     723    4533     497

Cuts at:  12597   13938 
   Size:       1341

  Fragments arranged by size: 

                4533    3001    1790    1601    1341     723     497     329
                 123
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     127   10261   12371   13938 
   Size:        127   10134    2110    1567

  Fragments arranged by size: 

               10134    2110    1567     127
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2386    2418   12799   12831   13210   13242   13938 
   Size:       2386      32   10381      32     379      32     696

  Fragments arranged by size: 

               10381    2386     696     379      32      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     808    2043    2442    2515    3925    5383    5743    7693 
   Size:        808    1235     399      73    1410    1458     360    1950

Cuts at:   7693    8152    8624    9909   10254   11565   11778   12789   13078 
   Size:        459     472    1285     345    1311     213    1011     289

Cuts at:  13078   13182   13938 
   Size:        104     756

  Fragments arranged by size: 

                1950    1458    1410    1311    1285    1235    1011     808
                 756     472     459     399     360     345     289     213
                 104      73
 
ApaI G_GGCC'C

Cuts at:      0    6674    8221   13938 
   Size:       6674    1547    5717

  Fragments arranged by size: 

                6674    5717    1547
 
ApaLI G'TGCA_C

Cuts at:      0   10874   13929   13938 
   Size:      10874    3055       9

  Fragments arranged by size: 

               10874    3055       9
 
ApoI r'AATT_y

Cuts at:      0     289     602     764    1222    1402    3049    3388    3421 
   Size:        289     313     162     458     180    1647     339      33

Cuts at:   3421    4083    4381    5593    9322   10792   11209   11710   12616 
   Size:        662     298    1212    3729    1470     417     501     906

Cuts at:  12616   13938 
   Size:       1322

  Fragments arranged by size: 

                3729    1647    1470    1322    1212     906     662     501
                 458     417     339     313     298     289     180     162
                  33
 
AvaI C'yCGr_G

Cuts at:      0      10    2139    2168    5033    6675    6730    8214   10990 
   Size:         10    2129      29    2865    1642      55    1484    2776

Cuts at:  10990   11042   11081   11615   13938 
   Size:         52      39     534    2323

  Fragments arranged by size: 

                2865    2776    2323    2129    1642    1484     534      55
                  52      39      29      10
 
BamHI G'GATC_C

Cuts at:      0     755    2993    6921   11046   11768   13218   13890   13938 
   Size:        755    2238    3928    4125     722    1450     672      48

  Fragments arranged by size: 

                4125    3928    2238    1450     755     722     672      48
            
 
BanI G'GyrC_C

Cuts at:      0     111     141    3008    5854    6435    7653    8245    9048 
   Size:        111      30    2867    2846     581    1218     592     803

Cuts at:   9048   11680   12399   13115   13404   13498   13648   13938 
   Size:       2632     719     716     289      94     150     290

  Fragments arranged by size: 

                2867    2846    2632    1218     803     719     716     592
                 581     290     289     150     111      94      30
 
BanII G_rGCy'C

Cuts at:      0      11     883    3566    6028    6674    6836    7369    8221 
   Size:         11     872    2683    2462     646     162     533     852

Cuts at:   8221    9149    9614   10232   11696   13616   13938 
   Size:        928     465     618    1464    1920     322

  Fragments arranged by size: 

                2683    2462    1920    1464     928     872     852     646
                 618     533     465     322     162      11
 
BbeI G_GCGC'C

Cuts at:      0     145   13119   13502   13652   13938 
   Size:        145   12974     383     150     286

  Fragments arranged by size: 

               12974     383     286     150     145
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     386     431     716    2511    3793    6259    6897    6994 
   Size:        386      45     285    1795    1282    2466     638      97

Cuts at:   6994    7903    8915   10669   13093   13938 
   Size:        909    1012    1754    2424     845

  Fragments arranged by size: 

                2466    2424    1795    1754    1282    1012     909     845
                 638     386     285      97      45
 
BbvCI CC'TCA_GC

Cuts at:      0    1523    8266    8758   12472   13938 
   Size:       1523    6743     492    3714    1466

  Fragments arranged by size: 

                6743    3714    1523    1466     492
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    4920    4954    8539    8573   12154   12188   13938 
   Size:       4920      34    3585      34    3581      34    1750

  Fragments arranged by size: 

                4920    3585    3581    1750      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    8352   10766   13938 
   Size:       8352    2414    3172

  Fragments arranged by size: 

                8352    3172    2414
 
BclI T'GATC_A

Cuts at:      0     635    2552    5474    7905   10763   10982   13016   13352 
   Size:        635    1917    2922    2431    2858     219    2034     336

Cuts at:  13352   13938 
   Size:        586

  Fragments arranged by size: 

                2922    2858    2431    2034    1917     635     586     336
                 219
 
BfrBI ATG'CAT

Cuts at:      0    1206   13938 
   Size:       1206   12732
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1639    3904    5689    6135    7828   10384   12957   13535 
   Size:       1639    2265    1785     446    1693    2556    2573     578

Cuts at:  13535   13938 
   Size:        403

  Fragments arranged by size: 

                2573    2556    2265    1785    1693    1639     578     446
                 403
 
BglII A'GATC_T

Cuts at:      0    2090    3222    3434    8237    8922    9840   11070   13938 
   Size:       2090    1132     212    4803     685     918    1230    2868

  Fragments arranged by size: 

                4803    2868    2090    1230    1132     918     685     212
            
 
BlpI GC'TnA_GC

Cuts at:      0    2505    5344    5515    7105   11812   13183   13938 
   Size:       2505    2839     171    1590    4707    1371     755

  Fragments arranged by size: 

                4707    2839    2505    1590    1371     755     171
 
Bme1580I G_kGCm'C

Cuts at:      0    1131    1192    1627    6674    8221    8478    9111   10878 
   Size:       1131      61     435    5047    1547     257     633    1767

Cuts at:  10878   11683   11912   12097   12531   13933   13938 
   Size:        805     229     185     434    1402       5

  Fragments arranged by size: 

                5047    1767    1547    1402    1131     805     633     435
                 434     257     229     185      61       5
 
BmgBI CAC'GTC

Cuts at:      0      78    6489    7283    9189   13106   13938 
   Size:         78    6411     794    1906    3917     832

  Fragments arranged by size: 

                6411    3917    1906     832     794      78
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2112    3789    3935    5738    8641   12604   12738   13938 
   Size:       2112    1677     146    1803    2903    3963     134    1200

  Fragments arranged by size: 

                3963    2903    2112    1803    1677    1200     146     134
            
 
BmtI G_CTAG'C

Cuts at:      0    9484   13938 
   Size:       9484    4454
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      51    3241    3483    5025    6255    7012   10445   13938 
   Size:         51    3190     242    1542    1230     757    3433    3493

  Fragments arranged by size: 

                3493    3433    3190    1542    1230     757     242      51
            
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     175     187     205     761    1740    4997    5908 
   Size:        145      30      12      18     556     979    3257     911

Cuts at:   5908    6518    6607    7348    7477    7529    7851    7865    8128 
   Size:        610      89     741     129      52     322      14     263

Cuts at:   8128    8458    9034    9364    9601    9706    9982   11329   11470 
   Size:        330     576     330     237     105     276    1347     141

Cuts at:  11470   11940   12346   12826   13627   13938 
   Size:        470     406     480     801     311

  Fragments arranged by size: 

                3257    1347     979     911     801     741     610     576
                 556     480     470     406     330     330     322     311
                 276     263     237     145     141     129     105      89
                  52      30      18      14      12
 
Bpu10I CC'TnA_GC

Cuts at:      0    1523    5543    8266    8758    9613   12472   13938 
   Size:       1523    4020    2723     492     855    2859    1466

  Fragments arranged by size: 

                4020    2859    2723    1523    1466     855     492
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1182    8771    9208    9684   11437   11745   13177   13938 
   Size:       1182    7589     437     476    1753     308    1432     761

  Fragments arranged by size: 

                7589    1753    1432    1182     761     476     437     308
            
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1675    4802    5281    7640    7861    8424    8434    9061 
   Size:       1675    3127     479    2359     221     563      10     627

Cuts at:   9061   10307   11377   13938 
   Size:       1246    1070    2561

  Fragments arranged by size: 

                3127    2561    2359    1675    1246    1070     627     563
                 479     221      10
 
BsaAI yAC'GTr

Cuts at:      0    2028    6846    8190    8754   12662   13524   13706   13938 
   Size:       2028    4818    1344     564    3908     862     182     232

  Fragments arranged by size: 

                4818    3908    2028    1344     862     564     232     182
            
 
BsaBI GATnn'nnATC

Cuts at:      0     791    6920    8921    9508   13938 
   Size:        791    6129    2001     587    4430

  Fragments arranged by size: 

                6129    4430    2001     791     587
 
BsaHI Gr'CG_yC

Cuts at:      0     142     260    1410    2735   10082   10265   10850   12949 
   Size:        142     118    1150    1325    7347     183     585    2099

Cuts at:  12949   13116   13499   13649   13938 
   Size:        167     383     150     289

  Fragments arranged by size: 

                7347    2099    1325    1150     585     383     289     183
                 167     150     142     118
 
BsaWI w'CCGG_w

Cuts at:      0     606    2249    6107   10418   12702   13938 
   Size:        606    1643    3858    4311    2284    1236

  Fragments arranged by size: 

                4311    3858    2284    1643    1236     606
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2808    2838    2969    2999    5033    5038    5063    5068 
   Size:       2808      30     131      30    2034       5      25       5

Cuts at:   5068    6578    6608    9907    9937   11374   11404   11464   11494 
   Size:       1510      30    3299      30    1437      30      60      30

Cuts at:  11494   11875   11905   12319   12349   12799   12829   13938 
   Size:        381      30     414      30     450      30    1109

  Fragments arranged by size: 

                3299    2808    2034    1510    1437    1109     450     414
                 381     131      60      30      30      30      30      30
                  30      30      30      30      25       5       5
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     184    2401    5059    5905    6553    7231    8536    9157 
   Size:        184    2217    2658     846     648     678    1305     621

Cuts at:   9157    9598   10084   11368   12922   13624   13938 
   Size:        441     486    1284    1554     702     314

  Fragments arranged by size: 

                2658    2217    1554    1305    1284     846     702     678
                 648     621     486     441     314     184
 
BseYI C'CCAG_C

Cuts at:      0    1817    2015    4466    4565    4694    5240    5697    5915 
   Size:       1817     198    2451      99     129     546     457     218

Cuts at:   5915    7539    9981   10510   11393   12960   13772   13938 
   Size:       1624    2442     529     883    1567     812     166

  Fragments arranged by size: 

                2451    2442    1817    1624    1567     883     812     546
                 529     457     218     198     166     129      99
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82    2137    2455    2653    2952    3538    3976    4639 
   Size:         82    2055     318     198     299     586     438     663

Cuts at:   4639    4682    5944    6317    7369   12707   12937   13123   13147 
   Size:         43    1262     373    1052    5338     230     186      24

Cuts at:  13147   13865   13938 
   Size:        718      73

  Fragments arranged by size: 

                5338    2055    1262    1052     718     663     586     438
                 373     318     299     230     198     186      82      73
                  43      24
 
BsiEI CG_ry'CG

Cuts at:      0      41      59     149    6135    7568   11997   13938 
   Size:         41      18      90    5986    1433    4429    1941

  Fragments arranged by size: 

                5986    4429    1941    1433      90      41      18
 
BsiHKAI G_wGCw'C

Cuts at:      0    3566    7111    7316    7334    8066    9149   10426   10878 
   Size:       3566    3545     205      18     732    1083    1277     452

Cuts at:  10878   12133   13616   13933   13938 
   Size:       1255    1483     317       5

  Fragments arranged by size: 

                3566    3545    1483    1277    1255    1083     732     452
                 317     205      18       5
 
BsmI GAATG_Cn'

Cuts at:      0     349     447    1734    3066    9477    9702    9885   12339 
   Size:        349      98    1287    1332    6411     225     183    2454

Cuts at:  12339   12488   13938 
   Size:        149    1450

  Fragments arranged by size: 

                6411    2454    1450    1332    1287     349     225     183
                 149      98
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    4515    6421    7666    8497    8934    9062   11350   12501 
   Size:       4515    1906    1245     831     437     128    2288    1151

Cuts at:  12501   13938 
   Size:       1437

  Fragments arranged by size: 

                4515    2288    1906    1437    1245    1151     831     437
                 128
 
Bsp1286I G_dGCh'C

Cuts at:      0      11     883    1131    1192    1627    3566    6028    6674 
   Size:         11     872     248      61     435    1939    2462     646

Cuts at:   6674    6836    7111    7316    7334    7369    8066    8221    8478 
   Size:        162     275     205      18      35     697     155     257

Cuts at:   8478    9111    9149    9614   10232   10426   10878   11683   11696 
   Size:        633      38     465     618     194     452     805      13

Cuts at:  11696   11912   12097   12133   12531   13616   13933   13938 
   Size:        216     185      36     398    1085     317       5

  Fragments arranged by size: 

                2462    1939    1085     872     805     697     646     633
                 618     465     452     435     398     317     275     257
                 248     216     205     194     185     162     155      61
                  38      36      35      18      13      11       5
 
BspEI T'CCGG_A

Cuts at:      0     606    2249    6107   13938 
   Size:        606    1643    3858    7831

  Fragments arranged by size: 

                7831    3858    1643     606
 
BspHI T'CATG_A

Cuts at:      0    2877    4247   13938 
   Size:       2877    1370    9691

  Fragments arranged by size: 

                9691    2877    1370
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     107     207    2380    2760    3693    3722    3776    3948 
   Size:        107     100    2173     380     933      29      54     172

Cuts at:   3948    4685    4826    5727    5910    9867   11943   12263   12641 
   Size:        737     141     901     183    3957    2076     320     378

Cuts at:  12641   13938 
   Size:       1297

  Fragments arranged by size: 

                3957    2173    2076    1297     933     901     737     380
                 378     320     183     172     141     107     100      54
                  29
 
BsrBI CCG'CTC

Cuts at:      0    1696    2391    4866    5895    8304   10016   11409   12072 
   Size:       1696     695    2475    1029    2409    1712    1393     663

Cuts at:  12072   13911   13938 
   Size:       1839      27

  Fragments arranged by size: 

                2475    2409    1839    1712    1696    1393    1029     695
                 663      27
 
BsrDI GCAATG_nn'

Cuts at:      0     468    2456    3579    5355    5825    6309    8603   10245 
   Size:        468    1988    1123    1776     470     484    2294    1642

Cuts at:  10245   10505   10538   13938 
   Size:        260      33    3400

  Fragments arranged by size: 

                3400    2294    1988    1776    1642    1123     484     470
                 468     260      33
 
BsrFI r'CCGG_y

Cuts at:      0     144    6071    6134    7517   13411   13938 
   Size:        144    5927      63    1383    5894     527

  Fragments arranged by size: 

                5927    5894    1383     527     144      63
 
BsrGI T'GTAC_A

Cuts at:      0    2496    3021    7742    8615   13938 
   Size:       2496     525    4721     873    5323

  Fragments arranged by size: 

                5323    4721    2496     873     525
 
BssSI C'ACGA_G

Cuts at:      0    9139    9562   13938 
   Size:       9139     423    4376

  Fragments arranged by size: 

                9139    4376     423
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    4680    5340    9594   11907   12345   13938 
   Size:       4680     660    4254    2313     438    1593

  Fragments arranged by size: 

                4680    4254    2313    1593     660     438
 
BstBI TT'CG_AA

Cuts at:      0   13871   13938 
   Size:      13871      67
 
BstEII G'GTnAC_C

Cuts at:      0    5358    7296   10888   11337   13812   13938 
   Size:       5358    1938    3592     449    2475     126

  Fragments arranged by size: 

                5358    3592    2475    1938     449     126
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     345    1047    3620    5767    7293    8125   10296   10842 
   Size:        345     702    2573    2147    1526     832    2171     546

Cuts at:  10842   11658   12599   13938 
   Size:        816     941    1339

  Fragments arranged by size: 

                2573    2171    2147    1526    1339     941     832     816
                 702     546     345
 
BstYI r'GATC_y

Cuts at:      0     755     792    1097    2090    2993    3222    3434    4829 
   Size:        755      37     305     993     903     229     212    1395

Cuts at:   4829    5828    6921    7241    8237    8922    9339    9840   10010 
   Size:        999    1093     320     996     685     417     501     170

Cuts at:  10010   11046   11070   11768   12710   13218   13373   13890   13938 
   Size:       1036      24     698     942     508     155     517      48

  Fragments arranged by size: 

                1395    1093    1036     999     996     993     942     903
                 755     698     685     517     508     501     417     320
                 305     229     212     170     155      48      37      24
            
 
Bsu36I CC'TnA_GG

Cuts at:      0    1450   13550   13938 
   Size:       1450   12100     388

  Fragments arranged by size: 

               12100    1450     388
 
BtgI C'CryG_G

Cuts at:      0    1040    1791    3746    4461    7639    8315    9926   11441 
   Size:       1040     751    1955     715    3178     676    1611    1515

Cuts at:  11441   11684   12008   12380   12715   13202   13862   13938 
   Size:        243     324     372     335     487     660      76

  Fragments arranged by size: 

                3178    1955    1611    1515    1040     751     715     676
                 660     487     372     335     324     243      76
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    6242    6929   10314   13938 
   Size:       6242     687    3385    3624

  Fragments arranged by size: 

                6242    3624    3385     687
 
BtsI GCAGTG_nn'

Cuts at:      0     473    1129    2657    3503    3948    4665    5932    5941 
   Size:        473     656    1528     846     445     717    1267       9

Cuts at:   5941    6179    7580    8306    9100   10424   12925   13135   13562 
   Size:        238    1401     726     794    1324    2501     210     427

Cuts at:  13562   13938 
   Size:        376

  Fragments arranged by size: 

                2501    1528    1401    1324    1267     846     794     726
                 717     656     473     445     427     376     238     210
                   9
 
ClaI AT'CG_AT

Cuts at:      0    4028    7604   13938 
   Size:       4028    3576    6334

  Fragments arranged by size: 

                6334    4028    3576
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2829    2864    6428    6463   13938 
   Size:       2829      35    3564      35    7475

  Fragments arranged by size: 

                7475    3564    2829      35      35
 
DraI TTT'AAA

Cuts at:      0    5058    6219   13938 
   Size:       5058    1161    7719

  Fragments arranged by size: 

                7719    5058    1161
 
DraIII CAC_nnn'GTG

Cuts at:      0     228     421    5340    5947    7683    8389    9192    9912 
   Size:        228     193    4919     607    1736     706     803     720

Cuts at:   9912   12090   13006   13938 
   Size:       2178     916     932

  Fragments arranged by size: 

                4919    2178    1736     932     916     803     720     706
                 607     228     193
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     217    2578    5635   13938 
   Size:        217    2361    3057    8303

  Fragments arranged by size: 

                8303    3057    2361     217
 
EaeI y'GGCC_r

Cuts at:      0      38      56     146    3479    3576    4293    4514    5138 
   Size:         38      18      90    3333      97     717     221     624

Cuts at:   5138    5757    6035    6116    7058    7136    7754    9035    9506 
   Size:        619     278      81     942      78     618    1281     471

Cuts at:   9506    9929   11949   12005   12677   13938 
   Size:        423    2020      56     672    1261

  Fragments arranged by size: 

                3333    2020    1281    1261     942     717     672     624
                 619     618     471     423     278     221      97      90
                  81      78      56      38      18
 
EagI C'GGCC_G

Cuts at:      0      38      56     146   13938 
   Size:         38      18      90   13792

  Fragments arranged by size: 

               13792      90      38      18
 
EarI CTCTTCn'nnn_

Cuts at:      0     169    1645    1859    2921    3038    3095    3815    3941 
   Size:        169    1476     214    1062     117      57     720     126

Cuts at:   3941    4483    4544    6246    6274    6304    6475    6682    6841 
   Size:        542      61    1702      28      30     171     207     159

Cuts at:   6841    7378    7819    8500    8654    9464   10432   10436   11314 
   Size:        537     441     681     154     810     968       4     878

Cuts at:  11314   11593   12376   13938 
   Size:        279     783    1562

  Fragments arranged by size: 

                1702    1562    1476    1062     968     878     810     783
                 720     681     542     537     441     279     214     207
                 171     169     159     154     126     117      61      57
                  30      28       4
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      88    5324    5430    7714   11809   13938 
   Size:         88    5236     106    2284    4095    2129

  Fragments arranged by size: 

                5236    4095    2284    2129     106      88
 
EcoICRI GAG'CTC

Cuts at:      0    3564    9147   13614   13938 
   Size:       3564    5583    4467     324

  Fragments arranged by size: 

                5583    4467    3564     324
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     175     187     205     524     761    1273    1357 
   Size:        145      30      12      18     319     237     512      84

Cuts at:   1357    1740    2065    2810    3148    3155    3814    3889    4997 
   Size:        383     325     745     338       7     659      75    1108

Cuts at:   4997    5908    6206    6456    6518    6565    6607    6733    7348 
   Size:        911     298     250      62      47      42     126     615

Cuts at:   7348    7477    7529    7800    7851    7865    7915    8128    8458 
   Size:        129      52     271      51      14      50     213     330

Cuts at:   8458    8584    8710    9034    9364    9601    9706    9982   10456 
   Size:        126     126     324     330     237     105     276     474

Cuts at:  10456   10839   11329   11338   11470   11557   11695   11940   12307 
   Size:        383     490       9     132      87     138     245     367

Cuts at:  12307   12346   12826   13051   13255   13428   13627   13938 
   Size:         39     480     225     204     173     199     311

  Fragments arranged by size: 

                1108     911     745     659     615     512     490     480
                 474     383     383     367     338     330     330     325
                 324     319     311     298     276     271     250     245
                 237     237     225     213     204     199     173     145
                 138     132     129     126     126     126     105      87
                  84      75      62      52      51      50      47      42
                  39      30      18      14      12       9       7
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     800    1931    2008    4999    5845    6535    6712    8453 
   Size:        800    1131      77    2991     846     690     177    1741

Cuts at:   8453    9913   11590   11977   13938 
   Size:       1460    1677     387    1961

  Fragments arranged by size: 

                2991    1961    1741    1677    1460    1131     846     800
                 690     387     177      77
 
EcoO109I rG'GnC_Cy

Cuts at:      0     408    1446    1494    2439    3981    5468    6670    6671 
   Size:        408    1038      48     945    1542    1487    1202       1

Cuts at:   6671    8024    8218   11583   11775   12168   13287   13938 
   Size:       1353     194    3365     192     393    1119     651

  Fragments arranged by size: 

                3365    1542    1487    1353    1202    1119    1038     945
                 651     408     393     194     192      48       1
 
EcoRI G'AATT_C

Cuts at:      0     289    3421    9322   10792   11710   13938 
   Size:        289    3132    5901    1470     918    2228

  Fragments arranged by size: 

                5901    3132    2228    1470     918     289
 
EcoRV GAT'ATC

Cuts at:      0     906    4668    7477   13938 
   Size:        906    3762    2809    6461

  Fragments arranged by size: 

                6461    3762    2809     906
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1588    2020    3366    3844    4470    4927    8728    9859 
   Size:       1588     432    1346     478     626     457    3801    1131

Cuts at:   9859   10499   11604   13938 
   Size:        640    1105    2334

  Fragments arranged by size: 

                3801    2334    1588    1346    1131    1105     640     626
                 478     457     432
 
FspI TGC'GCA

Cuts at:      0     103     196    7147   13345   13938 
   Size:        103      93    6951    6198     593

  Fragments arranged by size: 

                6951    6198     593     103      93
 
HaeII r_GCGC'y

Cuts at:      0     145     166     182     243     927    1069    2505    3585 
   Size:        145      21      16      61     684     142    1436    1080

Cuts at:   3585    3910    6894    6996    7309    7464    7823    8428    9961 
   Size:        325    2984     102     313     155     359     605    1533

Cuts at:   9961   11565   12813   13119   13502   13652   13938 
   Size:       1604    1248     306     383     150     286

  Fragments arranged by size: 

                2984    1604    1533    1436    1248    1080     684     605
                 383     359     325     313     306     286     155     150
                 145     142     102      61      21      16
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     784     816    1018    1050    1295    1327    1421    1453 
   Size:        784      32     202      32     245      32      94      32

Cuts at:   1453    1680    1712    1834    1866    2985    3017    4578    4610 
   Size:        227      32     122      32    1119      32    1561      32

Cuts at:   4610    4855    4887    5962    5994    6305    6337    7557    7589 
   Size:        245      32    1075      32     311      32    1220      32

Cuts at:   7589    7890    7922    8660    8692    9907    9939   10967   10999 
   Size:        301      32     738      32    1215      32    1028      32

Cuts at:  10999   11167   11199   11374   11406   11875   11907   12359   12391 
   Size:        168      32     175      32     469      32     452      32

Cuts at:  12391   12622   12654   13090   13122   13365   13397   13938 
   Size:        231      32     436      32     243      32     541

  Fragments arranged by size: 

                1561    1220    1215    1119    1075    1028     784     738
                 541     469     452     436     311     301     245     245
                 243     231     227     202     175     168     122      94
                  32      32      32      32      32      32      32      32
                  32      32      32      32      32      32      32      32
                  32      32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0     747    2211    2700    2808    4311    8782    9636   10056 
   Size:        747    1464     489     108    1503    4471     854     420

Cuts at:  10056   12864   13629   13938 
   Size:       2808     765     309

  Fragments arranged by size: 

                4471    2808    1503    1464     854     765     747     489
                 420     309     108
 
HindIII A'AGCT_T

Cuts at:      0    2704    4072    4113   13938 
   Size:       2704    1368      41    9825

  Fragments arranged by size: 

                9825    2704    1368      41
 
HpaI GTT'AAC

Cuts at:      0     747    2211   13938 
   Size:        747    1464   11727

  Fragments arranged by size: 

               11727    1464     747
 
KasI G'GCGC_C

Cuts at:      0     141   13115   13498   13648   13938 
   Size:        141   12974     383     150     290

  Fragments arranged by size: 

               12974     383     290     150     141
 
KpnI G_GTAC'C

Cuts at:      0    3012   13938 
   Size:       3012   10926
 
MfeI C'AATT_G

Cuts at:      0    2921    5195   10187   12203   13938 
   Size:       2921    2274    4992    2016    1735

  Fragments arranged by size: 

                4992    2921    2274    2016    1735
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    3437    5981    6464    7310    8512   11074   12390   13025 
   Size:       3437    2544     483     846    1202    2562    1316     635

Cuts at:  13025   13133   13178   13463   13938 
   Size:        108      45     285     475

  Fragments arranged by size: 

                3437    2562    2544    1316    1202     846     635     483
                 475     285     108      45
 
MscI TGG'CCA

Cuts at:      0    3578    4295    5140    5759    6037    6118    7060    7138 
   Size:       3578     717     845     619     278      81     942      78

Cuts at:   7138    9037    9508    9931   11951   12007   13938 
   Size:       1899     471     423    2020      56    1931

  Fragments arranged by size: 

                3578    2020    1931    1899     942     845     717     619
                 471     423     278      81      78      56
 
MslI CAynn'nnrTG

Cuts at:      0    1790    1913    3514    6049    6515    6844    7114    7567 
   Size:       1790     123    1601    2535     466     329     270     453

Cuts at:   7567    7846    9085   10024   11368   12100   12433   12504   12597 
   Size:        279    1239     939    1344     732     333      71      93

Cuts at:  12597   12883   13522   13938 
   Size:        286     639     416

  Fragments arranged by size: 

                2535    1790    1601    1344    1239     939     732     639
                 466     453     416     333     329     286     279     270
                 123      93      71
 
MspA1I CmG'CkG

Cuts at:      0     117     752    1821    1971    4107    4565    4698    5660 
   Size:        117     635    1069     150    2136     458     133     962

Cuts at:   5660    6465    7158    7185    7326    7455    7625    8724    9437 
   Size:        805     693      27     141     129     170    1099     713

Cuts at:   9437    9789   10399   10531   10761   11232   12259   12324   13464 
   Size:        352     610     132     230     471    1027      65    1140

Cuts at:  13464   13486   13598   13767   13938 
   Size:         22     112     169     171

  Fragments arranged by size: 

                2136    1140    1099    1069    1027     962     805     713
                 693     635     610     471     458     352     230     171
                 170     169     150     141     133     132     129     117
                 112      65      27      22
 
NaeI GCC'GGC

Cuts at:      0     146    7519   13413   13938 
   Size:        146    7373    5894     525

  Fragments arranged by size: 

                7373    5894     525     146
 
NarI GG'CG_CC

Cuts at:      0     142   13116   13499   13649   13938 
   Size:        142   12974     383     150     289

  Fragments arranged by size: 

               12974     383     289     150     142
 
NcoI C'CATG_G

Cuts at:      0    8315    9926   11441   12008   12380   13938 
   Size:       8315    1611    1515     567     372    1558

  Fragments arranged by size: 

                8315    1611    1558    1515     567     372
 
NdeI CA'TA_TG

Cuts at:      0    2493    6576   13938 
   Size:       2493    4083    7362

  Fragments arranged by size: 

                7362    4083    2493
 
NgoMIV G'CCGG_C

Cuts at:      0     144    7517   13411   13938 
   Size:        144    7373    5894     527

  Fragments arranged by size: 

                7373    5894     527     144
 
NheI G'CTAG_C

Cuts at:      0    9480   13938 
   Size:       9480    4458
 
NruI TCG'CGA

Cuts at:      0    1805   13938 
   Size:       1805   12133
 
NsiI A_TGCA'T

Cuts at:      0    1208   13938 
   Size:       1208   12730
 
NspI r_CATG'y

Cuts at:      0    1210    1247    9378   10116   10215   11625   12021   12163 
   Size:       1210      37    8131     738      99    1410     396     142

Cuts at:  12163   12358   12471   12857   13938 
   Size:        195     113     386    1081

  Fragments arranged by size: 

                8131    1410    1210    1081     738     396     386     195
                 142     113      99      37
 
PasI CC'CwG_GG

Cuts at:      0    4049   12307   13938 
   Size:       4049    8258    1631

  Fragments arranged by size: 

                8258    4049    1631
 
PciI A'CATG_T

Cuts at:      0   10112   10211   12017   12159   13938 
   Size:      10112      99    1806     142    1779

  Fragments arranged by size: 

               10112    1806    1779     142      99
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    3456    5247    5820    6821    7496    9528    9684   10001 
   Size:       3456    1791     573    1001     675    2032     156     317

Cuts at:  10001   10911   12090   13598   13938 
   Size:        910    1179    1508     340

  Fragments arranged by size: 

                3456    2032    1791    1508    1179    1001     910     675
                 573     340     317     156
 
PfoI T'CCnGG_A

Cuts at:      0     863    3731    9011    9779   10841   10904   11495   12803 
   Size:        863    2868    5280     768    1062      63     591    1308

Cuts at:  12803   13938 
   Size:       1135

  Fragments arranged by size: 

                5280    2868    1308    1135    1062     863     768     591
                  63
 
PmlI CAC'GTG

Cuts at:      0    2028    6846    8190    8754   12662   13524   13706   13938 
   Size:       2028    4818    1344     564    3908     862     182     232

  Fragments arranged by size: 

                4818    3908    2028    1344     862     564     232     182
            
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    6931    6963    9196    9228   12799   12831   13938 
   Size:       6931      32    2233      32    3571      32    1107

  Fragments arranged by size: 

                6931    3571    2233    1107      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     408    1446    1494    3981    5468    8024   11583   12168 
   Size:        408    1038      48    2487    1487    2556    3559     585

Cuts at:  12168   13287   13938 
   Size:       1119     651

  Fragments arranged by size: 

                3559    2556    2487    1487    1119    1038     651     585
                 408      48
 
PshAI GACnn'nnGTC

Cuts at:      0     959    6263   12785   13938 
   Size:        959    5304    6522    1153

  Fragments arranged by size: 

                6522    5304    1153     959
 
PsiI TTA'TAA

Cuts at:      0    8977   11575   13938 
   Size:       8977    2598    2363

  Fragments arranged by size: 

                8977    2598    2363
 
PspOMI G'GGCC_C

Cuts at:      0    6670    8217   13938 
   Size:       6670    1547    5721

  Fragments arranged by size: 

                6670    5721    1547
 
PspXI vC'TCGA_Gb

Cuts at:      0    6730   11615   13938 
   Size:       6730    4885    2323

  Fragments arranged by size: 

                6730    4885    2323
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    6046    6078   13938 
   Size:       6046      32    7860

  Fragments arranged by size: 

                7860    6046      32
 
PstI C_TGCA'G

Cuts at:      0      92    1379    1545    6887    7241   10068   10250   11334 
   Size:         92    1287     166    5342     354    2827     182    1084

Cuts at:  11334   11425   12222   12350   13469   13938 
   Size:         91     797     128    1119     469

  Fragments arranged by size: 

                5342    2827    1287    1119    1084     797     469     354
                 182     166     128      92      91
 
PvuI CG_AT'CG

Cuts at:      0   11997   13938 
   Size:      11997    1941
 
PvuII CAG'CTG

Cuts at:      0    1821    1971    4107    4565    7326    7455    9437    9789 
   Size:       1821     150    2136     458    2761     129    1982     352

Cuts at:   9789   10399   10531   11232   12324   13464   13486   13598   13767 
   Size:        610     132     701    1092    1140      22     112     169

Cuts at:  13767   13938 
   Size:        171

  Fragments arranged by size: 

                2761    2136    1982    1821    1140    1092     701     610
                 458     352     171     169     150     132     129     112
                  22
 
SacI G_AGCT'C

Cuts at:      0    3566    9149   13616   13938 
   Size:       3566    5583    4467     322

  Fragments arranged by size: 

                5583    4467    3566     322
 
SalI G'TCGA_C

Cuts at:      0    9634   12862   13938 
   Size:       9634    3228    1076

  Fragments arranged by size: 

                9634    3228    1076
 
SanDI GG'GwC_CC

Cuts at:      0    8024   13938 
   Size:       8024    5914
 
SapI GCTCTTCn'nnn_

Cuts at:      0     169    3941    6475    6682    6841    7378   10432   10436 
   Size:        169    3772    2534     207     159     537    3054       4

Cuts at:  10436   13938 
   Size:       3502

  Fragments arranged by size: 

                3772    3502    3054    2534     537     207     169     159
                   4
 
ScaI AGT'ACT

Cuts at:      0    2166   11147   11479   13938 
   Size:       2166    8981     332    2459

  Fragments arranged by size: 

                8981    2459    2166     332
 
SexAI A'CCwGG_T

Cuts at:      0    3011    9638    9908   10591   13938 
   Size:       3011    6627     270     683    3347

  Fragments arranged by size: 

                6627    3347    3011     683     270
 
SfcI C'TryA_G

Cuts at:      0      88    1375    1541    6883    7237   10064   10246   10606 
   Size:         88    1287     166    5342     354    2827     182     360

Cuts at:  10606   11330   11421   12218   12346   13465   13938 
   Size:        724      91     797     128    1119     473

  Fragments arranged by size: 

                5342    2827    1287    1119     797     724     473     360
                 354     182     166     128      91      88
 
SfoI GGC'GCC

Cuts at:      0     143   13117   13500   13650   13938 
   Size:        143   12974     383     150     288

  Fragments arranged by size: 

               12974     383     288     150     143
 
SmaI CCC'GGG

Cuts at:      0      12    8216   11044   11083   13938 
   Size:         12    8204    2828      39    2855

  Fragments arranged by size: 

                8204    2855    2828      39      12
 
SmlI C'TyrA_G

Cuts at:      0    1161    2197    6730    8786    9223    9663   11452   11615 
   Size:       1161    1036    4533    2056     437     440    1789     163

Cuts at:  11615   11724   13192   13938 
   Size:        109    1468     746

  Fragments arranged by size: 

                4533    2056    1789    1468    1161    1036     746     440
                 437     163     109
 
SphI G_CATG'C

Cuts at:      0    1247   11625   12358   12857   13938 
   Size:       1247   10378     733     499    1081

  Fragments arranged by size: 

               10378    1247    1081     733     499
 
SspI AAT'ATT

Cuts at:      0     597    2729    8774   13938 
   Size:        597    2132    6045    5164

  Fragments arranged by size: 

                6045    5164    2132     597
 
StuI AGG'CCT

Cuts at:      0    1782    7393    8459    9122   13938 
   Size:       1782    5611    1066     663    4816

  Fragments arranged by size: 

                5611    4816    1782    1066     663
 
StyI C'CwwG_G

Cuts at:      0    2004    2588    3899    4290    5684    6767    7055    7301 
   Size:       2004     584    1311     391    1394    1083     288     246

Cuts at:   7301    7649    8315    9926   10268   11441   12008   12036   12380 
   Size:        348     666    1611     342    1173     567      28     344

Cuts at:  12380   12395   12680   13502   13938 
   Size:         15     285     822     436

  Fragments arranged by size: 

                2004    1611    1394    1311    1173    1083     822     666
                 584     567     436     391     348     344     342     288
                 285     246      28      15
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    3007    4885    5608    5842    6253    6655    7023    7531 
   Size:       3007    1878     723     234     411     402     368     508

Cuts at:   7531    8088    8368    8394   11958   12267   12912   12958   13422 
   Size:        557     280      26    3564     309     645      46     464

Cuts at:  13422   13522   13938 
   Size:        100     416

  Fragments arranged by size: 

                3564    3007    1878     723     645     557     508     464
                 416     411     402     368     309     280     234     100
                  46      26
 
TatI w'GTAC_w

Cuts at:      0    2164    2496    2976    3021    4059    4344    4788    6161 
   Size:       2164     332     480      45    1038     285     444    1373

Cuts at:   6161    7742    7919    8615    8897   11145   11477   12536   13938 
   Size:       1581     177     696     282    2248     332    1059    1402

  Fragments arranged by size: 

                2248    2164    1581    1402    1373    1059    1038     696
                 480     444     332     332     285     282     177      45
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      35      67    1098    1130    2662    2694    2808    2840 
   Size:         35      32    1031      32    1532      32     114      32

Cuts at:   2840    6158    6190    6913    6945    8162    8194   11031   11063 
   Size:       3318      32     723      32    1217      32    2837      32

Cuts at:  11063   13938 
   Size:       2875

  Fragments arranged by size: 

                3318    2875    2837    1532    1217    1031     723     114
                  35      32      32      32      32      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1309    5383    7720   11365   11388   13938 
   Size:       1309    4074    2337    3645      23    2550

  Fragments arranged by size: 

                4074    3645    2550    2337    1309      23
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2406    3906    6649   12751   13005   13578   13938 
   Size:       2406    1500    2743    6102     254     573     360

  Fragments arranged by size: 

                6102    2743    2406    1500     573     360     254
 
XhoI C'TCGA_G

Cuts at:      0    6730   11615   13938 
   Size:       6730    4885    2323

  Fragments arranged by size: 

                6730    4885    2323
 
XmaI C'CCGG_G

Cuts at:      0      10    8214   11042   11081   13938 
   Size:         10    8204    2828      39    2857

  Fragments arranged by size: 

                8204    2857    2828      39      10
 
XmnI GAAnn'nnTTC

Cuts at:      0    1012    1256    4114    4957    6103    6617    8569   10582 
   Size:       1012     244    2858     843    1146     514    1952    2013

Cuts at:  10582   10744   13938 
   Size:        162    3194

  Fragments arranged by size: 

                3194    2858    2013    1952    1146    1012     843     514
                 244     162
 
ZraI GAC'GTC

Cuts at:      0     261   13938 
   Size:        261   13677
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI     AfeI    AflII   AflIII
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NruI     NsiI     NspI     PasI     PciI    PflMI     PfoI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII     SacI     SalI    SanDI     SapI     ScaI    SexAI
     SfcI     SfoI     SmaI     SmlI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AclI     AgeI     AscI     AseI    AsiSI    AvrII     BaeI    BsiWI
   BssHII  BstZ17I     FseI    FspAI     MluI     NotI     PacI     PmeI
    RsrII    SacII     SbfI     SfiI     SgfI    SgrAI    SnaBI     SpeI
     SrfI     SwaI     XbaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com