Restriction map for pF1KB6496
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# NO CUT ENZYMES (  133 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BalI        BamHI       
BbeI        BbsI        BbvCI       BcgI        BciVI       
BclI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BpuAI       BsaHI       BsaI        BseAI       
BsiWI       BsmFI       BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BssHII      BssSI       BstBI       BstEII      BstPI       
BstUI       Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HgaI        Hin1I       HindIII     KasI        
KpnI        Ksp632I     KspI        MluI        MluNI       
MroI        MscI        MvnI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmlI        SnaBI       SphI        Sse8387I    SwaI        
Tth111I     XhoI        XmnI        


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# ONE CUT ENZYMES (   60 enzymes) #
###################################

AccI         1051  GTATAC               GT^[AC][GT]AC
ApaI          540  GGGCCC               GGGCC^C
AvaI          863  CCCGGG               C^[CT]CG[GA]G
AvaII         527  GGACC                G^G[AT]CC
BanI           93  GGTGCC               G^G[CT][GA]CC
BbrPI         691  CACGTG               CAC^GTG
BpmI          707  CTGGAG               CTGGAG(16/14)
BsaAI         691  CACGTG               [CT]AC^GT[GA]
BsaBI         286  GATGGTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         164  ACCGGA               [AT]^CCGG[AT]
BseRI         761  GAGGAG               GAGGAG(10/8)
BsgI         1042  GTGCAG               GTGCAG(16/14)
BsiEI         162  CGACCG               CG[GA][CT]^CG
BsmBI         650  GAGACG               CGTCTC(1/5)
BsmI          583  GAATGC               GAATGC(1/-1)
BsoBI         863  CCCGGG               C^[CT]CG[GA]G
Bsp1407I      773  TGTACA               T^GTACA
BsrGI         773  TGTACA               T^GTACA
BsrI          807  ACTGG                ACTGG(1/-1)
Bst1107I     1051  GTATAC               GTA^TAC
BstXI         530  CCACCCAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1051  GTATAC               GTA^TAC
Cac8I         483  GCAGGC               GC[ACGT]^[ACGT]GC
CfoI          549  GCGC                 GCG^C
DdeI           16  CTGAG                C^T[ACGT]AG
DraII         540  GGGCCCT              [GA]G^G[ACGT]CC[CT]
DsaI          303  CCATGG               C^C[GA][CT]GG
EcoO109I      540  GGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       303  CCATGG               C^C[AT][AT]GG
HaeII         548  GGCGCT               [GA]GCGC^[CT]
HhaI          549  GCGC                 GCG^C
HinP1I        549  GCGC                 G^CGC
HincII        990  GTTAAC               GT[CT]^[GA]AC
HindII        990  GTTAAC               GT[CT]^[GA]AC
HpaI          990  GTTAAC               GTT^AAC
MaeII         692  ACGT                 A^CGT
MamI          286  GATGGTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          507  CAATTG               C^AATTG
MspA1I        535  CAGCTG               C[AC]G^C[GT]G
MunI          507  CAATTG               C^AATTG
NcoI          303  CCATGG               C^CATGG
PleI          802  GAGTC                GAGTC(4/5)
PmaCI         691  CACGTG               CAC^GTG
PmlI          691  CACGTG               CAC^GTG
PstI          148  CTGCAG               CTGCA^G
PvuII         535  CAGCTG               CAG^CTG
SacI          297  GAGCTC               GAGCT^C
SfaNI         522  GATGC                GCATC(5/9)
SmaI          863  CCCGGG               CCC^GGG
SpeI          600  ACTAGT               A^CTAGT
SspBI         773  TGTACA               T^GTACA
StuI          128  AGGCCT               AGG^CCT
StyI          303  CCATGG               C^C[AT][AT]GG
TaqI           99  TCGA                 T^CGA
TfiI          725  GAATC                G^A[AT]TC
TthHB8I        99  TCGA                 T^CGA
XbaI          411  TCTAGA               T^CTAGA
XcmI          534  CCAGCTGGGCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         863  CCCGGG               C^CCGGG
XmaI          863  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AcsI          196  AAATTC               [GA]^AATT[CT]
              444  AAATTT              
AlwI          178  GATCC                GGATC(4/5)
              204  GGATC               
ApoI          196  AAATTC               [GA]^AATT[CT]
              444  AAATTT              
AspHI          18  GAGCAC               G[AT]GC[AT]^C
              297  GAGCTC              
BcnI          863  CCCGG                CC^[GC]GG
              864  CCGGG               
BglII          83  AGATCT               A^GATCT
              408  AGATCT              
BsiHKAI        18  GAGCAC               G[AT]GC[AT]^C
              297  GAGCTC              
BstAPI         20  GCACCTTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              516  GCAAGAGATGC         
HinfI         725  GAATC                G^A[ACGT]TC
              802  GAGTC               
NciI          863  CCCGG                CC^[GC]GG
              864  CCGGG               
PflMI         201  CCAGGATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              564  CCAGGAGGTGG         
SfcI          132  CTATAG               C^T[GA][CT]AG
              148  CTGCAG              
SspI          379  AATATT               AAT^ATT
              747  AATATT              
TspRI         806  CACTG                CA[GC]TG[ACGT][ACGT]^
             1040  CAGTG               
Van91I        201  CCAGGATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              564  CCAGGAGGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   50 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       BanII       
BbvI        BfaI        BmyI        BsaJI       BsiYI       
BslI        BsmAI       Bsp1286I    BssKI       BstF5I      
BstNI       BstYI       Cfr13I      DpnII       EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HpaII       
HphI        ItaI        MaeI        MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       RsaI        Sau3AI      Sau96I      ScrFI       
Tru9I       TseI        Tsp45I      Tsp509I     XhoII       
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