Restriction map for pF1KB6496
 (Linear) (Six-base) MAPSORT of: KIBB6496.seq  Check: 3503  from: 1  to: 1074



KIBB6496.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AccI GT'mk_AC

Cuts at:      0    1052    1074 
   Size:       1052      22
 
AleI CACnn'nnGTG

Cuts at:      0     781    1074 
   Size:        781     293
 
AlwNI CAG_nnn'CTG

Cuts at:      0      15     207    1026    1074 
   Size:         15     192     819      48

  Fragments arranged by size: 

                 819     192      48      15
 
ApaI G_GGCC'C

Cuts at:      0     544    1074 
   Size:        544     530
 
ApoI r'AATT_y

Cuts at:      0     196     444    1074 
   Size:        196     248     630

  Fragments arranged by size: 

                 630     248     196
 
AvaI C'yCGr_G

Cuts at:      0     863    1074 
   Size:        863     211
 
BanI G'GyrC_C

Cuts at:      0      93    1074 
   Size:         93     981
 
BanII G_rGCy'C

Cuts at:      0     301     544     917     949    1074 
   Size:        301     243     373      32     125

  Fragments arranged by size: 

                 373     301     243     125      32
 
BglII A'GATC_T

Cuts at:      0      83     408    1074 
   Size:         83     325     666

  Fragments arranged by size: 

                 666     325      83
 
Bme1580I G_kGCm'C

Cuts at:      0     544    1074 
   Size:        544     530
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     728    1074 
   Size:        728     346
 
Bpu10I CC'TnA_GC

Cuts at:      0      16    1074 
   Size:         16    1058
 
BsaAI yAC'GTr

Cuts at:      0     693    1074 
   Size:        693     381
 
BsaBI GATnn'nnATC

Cuts at:      0     290    1074 
   Size:        290     784
 
BsaWI w'CCGG_w

Cuts at:      0     164    1074 
   Size:        164     910
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     776    1074 
   Size:        776     298
 
BseYI C'CCAG_C

Cuts at:      0     533     537    1074 
   Size:        533       4     537

  Fragments arranged by size: 

                 537     533       4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1063    1074 
   Size:       1063      11
 
BsiEI CG_ry'CG

Cuts at:      0     165    1074 
   Size:        165     909
 
BsiHKAI G_wGCw'C

Cuts at:      0      22     301    1074 
   Size:         22     279     773

  Fragments arranged by size: 

                 773     279      22
 
BsmI GAATG_Cn'

Cuts at:      0     589    1074 
   Size:        589     485
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     644    1074 
   Size:        644     430
 
Bsp1286I G_dGCh'C

Cuts at:      0      22     301     544     917     949    1074 
   Size:         22     279     243     373      32     125

  Fragments arranged by size: 

                 373     279     243     125      32      22
 
BsrGI T'GTAC_A

Cuts at:      0     773    1074 
   Size:        773     301
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      26     522    1074 
   Size:         26     496     552

  Fragments arranged by size: 

                 552     496      26
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     537    1074 
   Size:        537     537
 
BstYI r'GATC_y

Cuts at:      0      83     204     408    1074 
   Size:         83     121     204     666

  Fragments arranged by size: 

                 666     204     121      83
 
BstZ17I GTA'TAC

Cuts at:      0    1053    1074 
   Size:       1053      21
 
BtgI C'CryG_G

Cuts at:      0     303    1074 
   Size:        303     771
 
EcoICRI GAG'CTC

Cuts at:      0     299    1074 
   Size:        299     775
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     728    1074 
   Size:        728     346
 
EcoO109I rG'GnC_Cy

Cuts at:      0     541    1074 
   Size:        541     533
 
HaeII r_GCGC'y

Cuts at:      0     552    1074 
   Size:        552     522
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     214     246     998    1030    1074 
   Size:        214      32     752      32      44

  Fragments arranged by size: 

                 752     214      44      32      32
 
HincII GTy'rAC

Cuts at:      0     992    1074 
   Size:        992      82
 
HpaI GTT'AAC

Cuts at:      0     992    1074 
   Size:        992      82
 
MfeI C'AATT_G

Cuts at:      0     507    1074 
   Size:        507     567
 
MslI CAynn'nnrTG

Cuts at:      0     695     781     839    1074 
   Size:        695      86      58     235

  Fragments arranged by size: 

                 695     235      86      58
 
MspA1I CmG'CkG

Cuts at:      0     537    1074 
   Size:        537     537
 
NcoI C'CATG_G

Cuts at:      0     303    1074 
   Size:        303     771
 
PasI CC'CwG_GG

Cuts at:      0     544    1074 
   Size:        544     530
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     207     570    1074 
   Size:        207     363     504

  Fragments arranged by size: 

                 504     363     207
 
PfoI T'CCnGG_A

Cuts at:      0     200    1074 
   Size:        200     874
 
PmlI CAC'GTG

Cuts at:      0     693    1074 
   Size:        693     381
 
PspOMI G'GGCC_C

Cuts at:      0     540    1074 
   Size:        540     534
 
PstI C_TGCA'G

Cuts at:      0     152    1074 
   Size:        152     922
 
PvuII CAG'CTG

Cuts at:      0     537    1074 
   Size:        537     537
 
SacI G_AGCT'C

Cuts at:      0     301    1074 
   Size:        301     773
 
SfcI C'TryA_G

Cuts at:      0     132     148    1074 
   Size:        132      16     926

  Fragments arranged by size: 

                 926     132      16
 
SmaI CCC'GGG

Cuts at:      0     865    1074 
   Size:        865     209
 
SpeI A'CTAG_T

Cuts at:      0     600    1074 
   Size:        600     474
 
SspI AAT'ATT

Cuts at:      0     381     749    1074 
   Size:        381     368     325

  Fragments arranged by size: 

                 381     368     325
 
StuI AGG'CCT

Cuts at:      0     130    1074 
   Size:        130     944
 
StyI C'CwwG_G

Cuts at:      0     303    1074 
   Size:        303     771
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     199     547     758    1074 
   Size:        199     348     211     316

  Fragments arranged by size: 

                 348     316     211     199
 
TatI w'GTAC_w

Cuts at:      0     773    1074 
   Size:        773     301
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     434     466    1074 
   Size:        434      32     608

  Fragments arranged by size: 

                 608     434      32
 
XbaI T'CTAG_A

Cuts at:      0     411    1074 
   Size:        411     663
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     541    1074 
   Size:        541     533
 
XmaI C'CCGG_G

Cuts at:      0     863    1074 
   Size:        863     211
 

 Enzymes that do cut:

     AccI     AleI    AlwNI     ApaI     ApoI     AvaI     BanI    BanII
    BglII Bme1580I     BpmI   Bpu10I    BsaAI    BsaBI    BsaWI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BsrGI
   BstAPI    BstXI    BstYI  BstZ17I     BtgI  EcoICRI  Eco57MI EcoO109I
    HaeII    Hin4I   HincII     HpaI     MfeI     MslI   MspA1I     NcoI
     PasI    PflMI     PfoI     PmlI   PspOMI     PstI    PvuII     SacI
     SfcI     SmaI     SpeI     SspI     StuI     StyI    TaqII     TatI
     TstI     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI     BplI
    BpuEI     BsaI    BsaHI    BsaXI    BsiWI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI    BstBI   BstEII   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI  HindIII     KasI     KpnI     MluI     MmeI     MscI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI     PmeI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI
    SnaBI     SphI     SrfI     SwaI  Tth111I     XhoI     XmnI     ZraI
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