Restriction map for pF1KB8011
##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AgeI        AhdI        AocI        
ApaI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BciVI       BclI        
BfaI        BfrI        BglII       BlnI        BsaAI       
BsaHI       BsaWI       BseAI       BsiEI       BsiWI       
BsoBI       BspEI       BspHI       BspMI       BsrDI       
BsrFI       BssHII      BssSI       Bst1107I    BstEII      
BstPI       BstYI       BstZ17I     Bsu36I      Cfr10I      
CpoI        DraI        DraII       DraIII      DrdI        
EaeI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HgaI        
Hin1I       HindIII     HpaI        KasI        KpnI        
KspI        MaeI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        ScaI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SspI        SwaI        
Tth111I     XbaI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AcsI          344  AAATTC               [GA]^AATT[CT]
AflIII        447  ACATGT               A^C[GA][CT]GT
Alw44I        443  GTGCAC               G^TGCAC
AlwI         1212  GATCC                GGATC(4/5)
AlwNI         749  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI         6  AGCGCT               AGC^GCT
ApaLI         443  GTGCAC               G^TGCAC
ApoI          344  AAATTC               [GA]^AATT[CT]
AspHI         443  GTGCAC               G[AT]GC[AT]^C
BbsI          120  GAAGAC               GAAGAC(2/6)
BcgI          953  CGAACGCAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI         1232  GCCAGTCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         120  GAAGAC               GAAGAC(2/6)
BsaBI         283  GATGATAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI       443  GTGCAC               G[AT]GC[AT]^C
BsmBI        1174  GAGACG               CGTCTC(1/5)
BsmI          586  GCATTC               GAATGC(1/-1)
Bsp1407I     1010  TGTACA               T^GTACA
BspDI         788  ATCGAT               AT^CGAT
BspLU11I      447  ACATGT               A^CATGT
BsrBI         744  CCGCTC               CCGCTC(-3/-3)
BsrGI        1010  TGTACA               T^GTACA
BstAPI        634  GCAACTTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         412  TTCGAA               TT^CGAA
ClaI          788  ATCGAT               AT^CGAT
Eco47III        6  AGCGCT               AGC^GCT
EcoNI        1028  CCTTGGCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII        226  GTTGAC               GT[CT]^[GA]AC
HindII        226  GTTGAC               GT[CT]^[GA]AC
MaeII         949  ACGT                 A^CGT
MamI          283  GATGATAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI          803  TTAA                 T^TAA
NspV          412  TTCGAA               TT^CGAA
PflMI         139  CCAAAAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         826  GACTTCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          462  CTGCAG               CTGCA^G
PvuII         999  CAGCTG               CAG^CTG
SbfI          461  CCTGCAGG             CCTGCA^GG
SfcI          462  CTGCAG               C^T[GA][CT]AG
SfuI          412  TTCGAA               TT^CGAA
SphI         1145  GCATGC               GCATG^C
Sse8387I      461  CCTGCAGG             CCTGCA^GG
SspBI        1010  TGTACA               T^GTACA
StuI         1036  AGGCCT               AGG^CCT
TfiI          693  GAATC                G^A[AT]TC
Tru9I         803  TTAA                 T^TAA
Van91I        139  CCAAAAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccII          18  CGCG                 CG^CG
              940  CGCG                
BlpI          746  GCTCAGC              GC^T[ACGT]AGC
             1197  GCTCAGC             
BmyI          443  GTGCAC               G[AGT]GC[ACT]^C
              703  GGGCAC              
Bpu1102I      746  GCTCAGC              GC^T[ACGT]AGC
             1197  GCTCAGC             
BsaI          676  GAGACC               GGTCTC(1/5)
              771  GGTCTC              
BsgI          919  CTGCAC               GTGCAG(16/14)
              568  GTGCAG              
BsmFI         783  GTCCC                GGGAC(10/14)
             1093  GTCCC               
Bsp1286I      443  GTGCAC               G[AGT]GC[ACT]^C
              703  GGGCAC              
BstUI          18  CGCG                 CG^CG
              940  CGCG                
CelII         746  GCTCAGC              GC^T[ACGT]AGC
             1197  GCTCAGC             
DpnII         453  GATC                 ^GATC
             1212  GATC                
DsaI          338  CCATGG               C^C[GA][CT]GG
              438  CCATGG              
EarI          608  CTCTTC               CTCTTC(1/4)
             1171  GAAGAG              
FauI           19  GCGGG                CCCGC(4/6)
              941  GCGGG               
HaeII           6  AGCGCT               [GA]GCGC^[CT]
             1194  GGCGCT              
Ksp632I       608  CTCTTC               CTCTTC(1/4)
             1171  GAAGAG              
MboI          453  GATC                 ^GATC
             1212  GATC                
MspA1I        999  CAGCTG               C[AC]G^C[GT]G
             1140  CCGCTG              
MvnI           18  CGCG                 CG^CG
              940  CGCG                
NcoI          338  CCATGG               C^CATGG
              438  CCATGG              
NdeII         453  GATC                 ^GATC
             1212  GATC                
NlaIV         258  GGACCC               GG[ACGT]^[ACGT]CC
             1184  GGACCC              
NspI          447  ACATGT               [GA]CATG^[CT]
             1145  GCATGC              
Sau3AI        453  GATC                 ^GATC
             1212  GATC                
SfaNI         867  GATGC                GCATC(5/9)
              963  GCATC               
Tsp509I       345  AATT                 ^AATT
              397  AATT                
XcmI          662  CCAAAGATGGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1215  CCAAGAACGGCTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AciI        AfaI        AluI        AvaII       BbvI        
BcnI        BpmI        BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsrI        BssKI       BstF5I      
BstNI       BstXI       Cac8I       CfoI        Cfr13I      
DdeI        EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeIII      MboII       
MnlI        MslI        MspI        MvaI        MwoI        
NciI        NlaIII      PleI        RsaI        Sau96I      
ScrFI       StyI        TaqI        TseI        Tsp45I      
TspRI       TthHB8I     
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