Restriction map for pF1KB3134
##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

AatII       AccI        AccII       AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        AspEI       AviII       
AvrII       BbeI        BbrPI       BcgI        BciVI       
BfrI        BglI        BlnI        BsaAI       BsiEI       
BsiWI       BsmBI       BspDI       BspLU11I    BsrBI       
BssHII      Bst1107I    BstUI       BstZ17I     ClaI        
CpoI        DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoT22I     FauI        FseI        
FspI        HaeII       HpaI        KasI        KspI        
MfeI        MluI        MunI        MvnI        NarI        
NdeI        NotI        NruI        NsiI        PacI        
PinAI       PmaCI       PmeI        PmlI        Psp1406I    
PvuI        RsrII       SacII       SalI        SapI        
SbfI        SexAI       SfiI        SfoI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SwaI        
XbaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

Acc65I         24  GGTACC               G^GTACC
AccIII       2408  TCCGGA               T^CCGGA
AcyI          188  GGCGTC               G[GA]^CG[CT]C
AocI         1297  CCTGAGG              CC^T[ACGT]AGG
AseI         2071  ATTAAT               AT^TAAT
AsnI         2071  ATTAAT               AT^TAAT
Asp718I        24  GGTACC               G^GTACC
AspI         1717  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          497  TGGCCA               TGG^CCA
BsaHI         188  GGCGTC               G[GA]^CG[CT]C
BsaI         1411  GAGACC               GGTCTC(1/5)
BseAI        2408  TCCGGA               T^CCGGA
Bsp1407I     1158  TGTACA               T^GTACA
BspEI        2408  TCCGGA               T^CCGGA
BsrFI        1106  GCCGGC               [GA]^CCGG[CT]
BsrGI        1158  TGTACA               T^GTACA
BssSI        1846  CACGAG               CACGAG(-5/-1)
BstBI        2759  TTCGAA               TT^CGAA
BstEII        121  GGTGACC              G^GT[ACGT]ACC
BstPI         121  GGTGACC              G^GT[ACGT]ACC
BstXI         488  CCAAGCAGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1297  CCTGAGG              CC^T[ACGT]AGG
Cfr10I       1106  GCCGGC               [GA]^CCGG[CT]
DraI         2215  TTTAAA               TTT^AAA
DraIII       2515  CACAGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI         2458  CTCTTC               CTCTTC(1/4)
Eco81I       1297  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       121  GGTGACC              G^GT[ACGT]ACC
EcoRI         287  GAATTC               G^AATTC
Hin1I         188  GGCGTC               G[GA]^CG[CT]C
KpnI           24  GGTACC               GGTAC^C
Ksp632I      2458  CTCTTC               CTCTTC(1/4)
MluNI         497  TGGCCA               TGG^CCA
MroI         2408  TCCGGA               T^CCGGA
MscI          497  TGGCCA               TGG^CCA
NaeI         1106  GCCGGC               GCC^GGC
NgoMIV       1106  GCCGGC               G^CCGGC
NspI         2005  GCATGT               [GA]CATG^[CT]
NspV         2759  TTCGAA               TT^CGAA
PaeR7I       1543  CTCGAG               C^TCGAG
PflFI        1717  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshBI        2071  ATTAAT               AT^TAAT
ScaI         1210  AGTACT               AGT^ACT
SfuI         2759  TTCGAA               TT^CGAA
SmaI         3239  CCCGGG               CCC^GGG
SspBI        1158  TGTACA               T^GTACA
StuI         3183  AGGCCT               AGG^CCT
Tth111I      1717  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         1543  CTCGAG               C^TCGAG
XmaCI        3239  CCCGGG               C^CCGGG
XmaI         3239  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AvaI         1543  CTCGAG               C^[CT]CG[GA]G
             3239  CCCGGG              
BamHI        1492  GGATCC               G^GATCC
             2198  GGATCC              
BanI           24  GGTACC               G^G[CT][GA]CC
             1113  GGCACC              
BlpI          394  GCTGAGC              GC^T[ACGT]AGC
             3247  GCTGAGC             
Bpu1102I      394  GCTGAGC              GC^T[ACGT]AGC
             3247  GCTGAGC             
BsaWI         329  ACCGGA               [AT]^CCGG[AT]
             2408  TCCGGA              
BsmI          635  GCATTC               GAATGC(1/-1)
             1637  GCATTC              
BsoBI        1543  CTCGAG               C^[CT]CG[GA]G
             3239  CCCGGG              
BspHI        1205  TCATGA               T^CATGA
             3665  TCATGA              
BsrDI        3255  CATTGC               GCAATG(2/0)
             2930  GCAATG              
BstAPI       2005  GCATGTGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2530  GCAGAAACTGC         
CelII         394  GCTGAGC              GC^T[ACGT]AGC
             3247  GCTGAGC             
EaeI          497  TGGCCA               [CT]^GGCC[GA]
             1706  TGGCCG              
EcoRV         133  GATATC               GAT^ATC
             2056  GATATC              
HincII       2125  GTCAAC               GT[CT]^[GA]AC
             3278  GTCAAC              
HindII       2125  GTCAAC               GT[CT]^[GA]AC
             3278  GTCAAC              
HindIII       424  AAGCTT               A^AGCTT
             1693  AAGCTT              
NheI         2902  GCTAGC               G^CTAGC
             2910  GCTAGC              
PshAI        1750  GACTTTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             3445  GACAGCAGTC          
RcaI         1205  TCATGA               T^CATGA
             3665  TCATGA              
SacI         1888  GAGCTC               GAGCT^C
             3250  GAGCTC              
SspI         1230  AATATT               AAT^ATT
             2693  AATATT              
XcmI         1445  CCACGGATGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1743  CCACAGGGACTTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  103 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        Asp700I     AspHI       AvaII       
BanII       BbsI        BbvCI       BbvI        BclI        
BcnI        BfaI        BglII       BmyI        BpmI        
BpuAI       BsaBI       BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FbaI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeI        MaeII       MaeIII      MamI        MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XhoII       XmnI        
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